(Linear) (Six-base) MAPSORT of: KIEE5126.seq  Check: 9934  from: 1  to: 423



KIEE5126.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     423 
   Size:        115     308
 
ApoI r'AATT_y

Cuts at:      0      38      66     189     423 
   Size:         38      28     123     234

  Fragments arranged by size: 

                 234     123      38      28
 
AseI AT'TA_AT

Cuts at:      0      17     423 
   Size:         17     406
 
BamHI G'GATC_C

Cuts at:      0     141     423 
   Size:        141     282
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      29     103     423 
   Size:         29      74     320

  Fragments arranged by size: 

                 320      74      29
 
BfrBI ATG'CAT

Cuts at:      0     388     423 
   Size:        388      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     423 
   Size:        356      67
 
BssSI C'ACGA_G

Cuts at:      0      43     423 
   Size:         43     380
 
BstYI r'GATC_y

Cuts at:      0     141     423 
   Size:        141     282
 
Bsu36I CC'TnA_GG

Cuts at:      0     233     423 
   Size:        233     190
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     105     423 
   Size:        105     318
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     423 
   Size:        115     308
 
EcoRI G'AATT_C

Cuts at:      0      66     423 
   Size:         66     357
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      37      69     423 
   Size:         37      32     354

  Fragments arranged by size: 

                 354      37      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     423 
   Size:        119     304
 
NsiI A_TGCA'T

Cuts at:      0     390     423 
   Size:        390      33
 
StyI C'CwwG_G

Cuts at:      0     219     423 
   Size:        219     204
 
XbaI T'CTAG_A

Cuts at:      0     115     423 
   Size:        115     308
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      78     423 
   Size:         78     345
 

 Enzymes that do cut:

     AcuI     ApoI     AseI    BamHI     BbsI    BfrBI     BsgI    BssSI
    BstYI   Bsu36I     DrdI  Eco57MI    EcoRI    Hin4I     MmeI     NsiI
     StyI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XhoI     XmaI     XmnI
     ZraI