Restriction map for
pFN21AE5119
(Linear) (Six-base) MAPSORT of: KIEE5119.seq Check: 354 from: 1 to: 462
KIEE5119.orf
With 182 enzymes: SgfI *
January 29, 2014 11:46 ..
AccI GT'mk_AC
Cuts at: 0 130 462
Size: 130 332
Acc65I G'GTAC_C
Cuts at: 0 290 462
Size: 290 172
BanI G'GyrC_C
Cuts at: 0 290 462
Size: 290 172
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 462
Size: 145 317
BbvCI CC'TCA_GC
Cuts at: 0 42 462
Size: 42 420
BclI T'GATC_A
Cuts at: 0 401 462
Size: 401 61
BlpI GC'TnA_GC
Cuts at: 0 66 462
Size: 66 396
BmrI ACTGGGnnnn_n'
Cuts at: 0 203 462
Size: 203 259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 462
Size: 289 173
Bpu10I CC'TnA_GC
Cuts at: 0 42 462
Size: 42 420
BsaI GGTCTCn'nnnn_
Cuts at: 0 344 462
Size: 344 118
BsaAI yAC'GTr
Cuts at: 0 76 462
Size: 76 386
BsaBI GATnn'nnATC
Cuts at: 0 277 400 462
Size: 277 123 62
Fragments arranged by size:
277 123 62
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 462
Size: 442 20
BsiHKAI G_wGCw'C
Cuts at: 0 67 98 462
Size: 67 31 364
Fragments arranged by size:
364 67 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 343 462
Size: 343 119
Bsp1286I G_dGCh'C
Cuts at: 0 67 98 462
Size: 67 31 364
Fragments arranged by size:
364 67 31
BsrFI r'CCGG_y
Cuts at: 0 104 462
Size: 104 358
BstAPI GCAn_nnn'nTGC
Cuts at: 0 439 462
Size: 439 23
BstBI TT'CG_AA
Cuts at: 0 136 462
Size: 136 326
BstXI CCAn_nnnn'nTGG
Cuts at: 0 175 274 462
Size: 175 99 188
Fragments arranged by size:
188 175 99
BstYI r'GATC_y
Cuts at: 0 395 462
Size: 395 67
Bsu36I CC'TnA_GG
Cuts at: 0 344 462
Size: 344 118
BtgI C'CryG_G
Cuts at: 0 452 462
Size: 452 10
BtsI GCAGTG_nn'
Cuts at: 0 67 298 462
Size: 67 231 164
Fragments arranged by size:
231 164 67
EaeI y'GGCC_r
Cuts at: 0 106 153 462
Size: 106 47 309
Fragments arranged by size:
309 106 47
EarI CTCTTCn'nnn_
Cuts at: 0 386 462
Size: 386 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 462
Size: 289 173
EcoNI CCTnn'n_nnAGG
Cuts at: 0 358 462
Size: 358 104
EcoO109I rG'GnC_Cy
Cuts at: 0 38 330 462
Size: 38 292 132
Fragments arranged by size:
292 132 38
EcoRV GAT'ATC
Cuts at: 0 213 462
Size: 213 249
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 102 462
Size: 102 360
FseI GG_CCGG'CC
Cuts at: 0 108 462
Size: 108 354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 380 412 462
Size: 380 32 50
Fragments arranged by size:
380 50 32
HincII GTy'rAC
Cuts at: 0 224 462
Size: 224 238
KpnI G_GTAC'C
Cuts at: 0 294 462
Size: 294 168
MspA1I CmG'CkG
Cuts at: 0 270 314 436 462
Size: 270 44 122 26
Fragments arranged by size:
270 122 44 26
NaeI GCC'GGC
Cuts at: 0 106 462
Size: 106 356
NcoI C'CATG_G
Cuts at: 0 452 462
Size: 452 10
NgoMIV G'CCGG_C
Cuts at: 0 104 462
Size: 104 358
PfoI T'CCnGG_A
Cuts at: 0 281 305 462
Size: 281 24 157
Fragments arranged by size:
281 157 24
PpuMI rG'GwC_Cy
Cuts at: 0 330 462
Size: 330 132
PshAI GACnn'nnGTC
Cuts at: 0 335 462
Size: 335 127
PvuII CAG'CTG
Cuts at: 0 270 436 462
Size: 270 166 26
Fragments arranged by size:
270 166 26
SnaBI TAC'GTA
Cuts at: 0 76 462
Size: 76 386
StyI C'CwwG_G
Cuts at: 0 161 354 452 462
Size: 161 193 98 10
Fragments arranged by size:
193 161 98 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 168 462
Size: 168 294
XmnI GAAnn'nnTTC
Cuts at: 0 262 462
Size: 262 200
Enzymes that do cut:
AccI Acc65I BanI BbsI BbvCI BclI BlpI BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BseRI BsiHKAI BsmBI
Bsp1286I BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtsI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FalI
FseI Hin4I HincII KpnI MspA1I NaeI NcoI NgoMIV
PfoI PpuMI PshAI PvuII SnaBI StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII
BbeI BcgI BciVI BfrBI BglI BglII Bme1580I BmgBI
BmtI BplI BpuEI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoRI FspI
FspAI HaeII HindIII HpaI KasI MfeI MluI MmeI
MscI MslI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI ZraI