Restriction map for
pFN21AE5118
(Linear) (Six-base) MAPSORT of: KIEE5118.seq Check: 817 from: 1 to: 435
KIEE5118.orf
With 182 enzymes: SgfI *
January 29, 2014 11:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 113 435
Size: 113 322
AatII G_ACGT'C
Cuts at: 0 317 435
Size: 317 118
AccI GT'mk_AC
Cuts at: 0 414 435
Size: 414 21
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 435
Size: 247 188
AleI CACnn'nnGTG
Cuts at: 0 107 435
Size: 107 328
AlwNI CAG_nnn'CTG
Cuts at: 0 183 203 435
Size: 183 20 232
Fragments arranged by size:
232 183 20
ApaI G_GGCC'C
Cuts at: 0 15 151 435
Size: 15 136 284
Fragments arranged by size:
284 136 15
ApoI r'AATT_y
Cuts at: 0 324 435
Size: 324 111
AvaI C'yCGr_G
Cuts at: 0 278 435
Size: 278 157
BanII G_rGCy'C
Cuts at: 0 15 76 102 151 435
Size: 15 61 26 49 284
Fragments arranged by size:
284 61 49 26 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 51 435
Size: 51 384
BglII A'GATC_T
Cuts at: 0 389 435
Size: 389 46
Bme1580I G_kGCm'C
Cuts at: 0 15 151 435
Size: 15 136 284
Fragments arranged by size:
284 136 15
BsaHI Gr'CG_yC
Cuts at: 0 314 435
Size: 314 121
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 348 435
Size: 130 218 87
Fragments arranged by size:
218 130 87
BseYI C'CCAG_C
Cuts at: 0 183 383 435
Size: 183 200 52
Fragments arranged by size:
200 183 52
Bsp1286I G_dGCh'C
Cuts at: 0 15 76 102 151 435
Size: 15 61 26 49 284
Fragments arranged by size:
284 61 49 26 15
BspHI T'CATG_A
Cuts at: 0 329 435
Size: 329 106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 113 435
Size: 113 322
BssSI C'ACGA_G
Cuts at: 0 364 435
Size: 364 71
BstEII G'GTnAC_C
Cuts at: 0 157 435
Size: 157 278
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 435
Size: 39 396
BstYI r'GATC_y
Cuts at: 0 389 435
Size: 389 46
BstZ17I GTA'TAC
Cuts at: 0 415 435
Size: 415 20
Bsu36I CC'TnA_GG
Cuts at: 0 403 435
Size: 403 32
DrdI GACnn_nn'nnGTC
Cuts at: 0 297 435
Size: 297 138
EaeI y'GGCC_r
Cuts at: 0 368 435
Size: 368 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 435
Size: 247 188
EcoNI CCTnn'n_nnAGG
Cuts at: 0 139 422 435
Size: 139 283 13
Fragments arranged by size:
283 139 13
EcoO109I rG'GnC_Cy
Cuts at: 0 12 147 148 200 360 399 427 435
Size: 12 135 1 52 160 39 28 8
Fragments arranged by size:
160 135 52 39 28 12 8 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 324 356 435
Size: 106 32 186 32 79
Fragments arranged by size:
186 106 79 32 32
HincII GTy'rAC
Cuts at: 0 167 435
Size: 167 268
MslI CAynn'nnrTG
Cuts at: 0 107 435
Size: 107 328
PasI CC'CwG_GG
Cuts at: 0 153 357 435
Size: 153 204 78
Fragments arranged by size:
204 153 78
PpuMI rG'GwC_Cy
Cuts at: 0 200 399 427 435
Size: 200 199 28 8
Fragments arranged by size:
200 199 28 8
PshAI GACnn'nnGTC
Cuts at: 0 214 435
Size: 214 221
PspOMI G'GGCC_C
Cuts at: 0 11 147 435
Size: 11 136 288
Fragments arranged by size:
288 136 11
PstI C_TGCA'G
Cuts at: 0 322 435
Size: 322 113
ScaI AGT'ACT
Cuts at: 0 82 435
Size: 82 353
SfcI C'TryA_G
Cuts at: 0 195 318 422 435
Size: 195 123 104 13
Fragments arranged by size:
195 123 104 13
SmaI CCC'GGG
Cuts at: 0 280 435
Size: 280 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 435
Size: 265 170
TatI w'GTAC_w
Cuts at: 0 80 435
Size: 80 355
XbaI T'CTAG_A
Cuts at: 0 346 435
Size: 346 89
XmaI C'CCGG_G
Cuts at: 0 278 435
Size: 278 157
XmnI GAAnn'nnTTC
Cuts at: 0 261 435
Size: 261 174
ZraI GAC'GTC
Cuts at: 0 315 435
Size: 315 120
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlwNI ApaI ApoI
AvaI BanII BbsI BglII Bme1580I BsaHI BseRI BseYI
Bsp1286I BspHI BspMI BssSI BstEII BstXI BstYI BstZ17I
Bsu36I DrdI EaeI Eco57MI EcoNI EcoO109I Hin4I HincII
MslI PasI PpuMI PshAI PspOMI PstI ScaI SfcI
SmaI TaqII TatI XbaI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BstAPI BstBI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI Tth111I XcmI XhoI