(Linear) (Six-base) MAPSORT of: KIEE5114.seq  Check: 5934  from: 1  to: 354



KIEE5114.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     187     354 
   Size:        187     167
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      98     354 
   Size:         98     256
 
BseYI C'CCAG_C

Cuts at:      0     302     354 
   Size:        302      52
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     310     354 
   Size:        310      44
 
BtsI GCAGTG_nn'

Cuts at:      0       8     354 
   Size:          8     346
 
ClaI AT'CG_AT

Cuts at:      0     231     354 
   Size:        231     123
 
EaeI y'GGCC_r

Cuts at:      0     217     354 
   Size:        217     137
 
EarI CTCTTCn'nnn_

Cuts at:      0      29     354 
   Size:         29     325
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     217     249     354 
   Size:        217      32     105

  Fragments arranged by size: 

                 217     105      32
 
MfeI C'AATT_G

Cuts at:      0     113     354 
   Size:        113     241
 
MscI TGG'CCA

Cuts at:      0     219     354 
   Size:        219     135
 
MspA1I CmG'CkG

Cuts at:      0     333     354 
   Size:        333      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     242     354 
   Size:        242     112
 
XmnI GAAnn'nnTTC

Cuts at:      0      20     354 
   Size:         20     334
 

 Enzymes that do cut:

     AlfI    BseRI    BseYI    BstXI     BtsI     ClaI     EaeI     EarI
    Hin4I     MfeI     MscI   MspA1I    PflMI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI