(Linear) (Six-base) MAPSORT of: KIEE5113.seq  Check: 7646  from: 1  to: 474



KIEE5113.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AatII G_ACGT'C

Cuts at:      0     359     474 
   Size:        359     115
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333     474 
   Size:        333     141
 
AflIII A'CryG_T

Cuts at:      0     323     474 
   Size:        323     151
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     427     474 
   Size:        427      47
 
BamHI G'GATC_C

Cuts at:      0     212     474 
   Size:        212     262
 
BanI G'GyrC_C

Cuts at:      0     157     448     474 
   Size:        157     291      26

  Fragments arranged by size: 

                 291     157      26
 
Bme1580I G_kGCm'C

Cuts at:      0     160     474 
   Size:        160     314
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     474 
   Size:        412      62
 
BsaHI Gr'CG_yC

Cuts at:      0     356     474 
   Size:        356     118
 
BseYI C'CCAG_C

Cuts at:      0      72     474 
   Size:         72     402
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     474 
   Size:        262     212
 
BsiHKAI G_wGCw'C

Cuts at:      0     428     474 
   Size:        428      46
 
Bsp1286I G_dGCh'C

Cuts at:      0     160     428     474 
   Size:        160     268      46

  Fragments arranged by size: 

                 268     160      46
 
BsrFI r'CCGG_y

Cuts at:      0     392     445     474 
   Size:        392      53      29

  Fragments arranged by size: 

                 392      53      29
 
BsrGI T'GTAC_A

Cuts at:      0     330     474 
   Size:        330     144
 
BstEII G'GTnAC_C

Cuts at:      0     228     432     474 
   Size:        228     204      42

  Fragments arranged by size: 

                 228     204      42
 
BstYI r'GATC_y

Cuts at:      0     212     474 
   Size:        212     262
 
BtgI C'CryG_G

Cuts at:      0     100     474 
   Size:        100     374
 
EarI CTCTTCn'nnn_

Cuts at:      0      55     474 
   Size:         55     419
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     225     253     474 
   Size:         19     206      28     221

  Fragments arranged by size: 

                 221     206      28      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     333     474 
   Size:        333     141
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     348     474 
   Size:        348     126
 
EcoO109I rG'GnC_Cy

Cuts at:      0      66     474 
   Size:         66     408
 
HaeII r_GCGC'y

Cuts at:      0     315     474 
   Size:        315     159
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     197     229     474 
   Size:        197      32     245

  Fragments arranged by size: 

                 245     197      32
 
MspA1I CmG'CkG

Cuts at:      0     102     474 
   Size:        102     372
 
NaeI GCC'GGC

Cuts at:      0     394     447     474 
   Size:        394      53      27

  Fragments arranged by size: 

                 394      53      27
 
NgoMIV G'CCGG_C

Cuts at:      0     392     445     474 
   Size:        392      53      29

  Fragments arranged by size: 

                 392      53      29
 
NspI r_CATG'y

Cuts at:      0     327     474 
   Size:        327     147
 
PciI A'CATG_T

Cuts at:      0     323     474 
   Size:        323     151
 
PpuMI rG'GwC_Cy

Cuts at:      0      66     474 
   Size:         66     408
 
PshAI GACnn'nnGTC

Cuts at:      0     256     474 
   Size:        256     218
 
SacII CC_GC'GG

Cuts at:      0     103     474 
   Size:        103     371
 
SmlI C'TyrA_G

Cuts at:      0     427     474 
   Size:        427      47
 
StyI C'CwwG_G

Cuts at:      0     140     437     474 
   Size:        140     297      37

  Fragments arranged by size: 

                 297     140      37
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     109     153     474 
   Size:        109      44     321

  Fragments arranged by size: 

                 321     109      44
 
TatI w'GTAC_w

Cuts at:      0     293     330     474 
   Size:        293      37     144

  Fragments arranged by size: 

                 293     144      37
 
Tth111I GACn'n_nGTC

Cuts at:      0     373     474 
   Size:        373     101
 
XmnI GAAnn'nnTTC

Cuts at:      0     460     474 
   Size:        460      14
 
ZraI GAC'GTC

Cuts at:      0     357     474 
   Size:        357     117
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AlfI    BamHI     BanI Bme1580I    BpuEI
    BsaHI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrFI    BsrGI   BstEII
    BstYI     BtgI     EarI     EciI  Eco57MI    EcoNI EcoO109I    HaeII
    Hin4I   MspA1I     NaeI   NgoMIV     NspI     PciI    PpuMI    PshAI
    SacII     SmlI     StyI    TaqII     TatI  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI    BstBI
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
     XbaI     XcmI     XhoI     XmaI