(Linear) (Six-base) MAPSORT of: KIEE5103.seq  Check: 3896  from: 1  to: 309



KIEE5103.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     149     309 
   Size:         73      76     160

  Fragments arranged by size: 

                 160      76      73
 
AflIII A'CryG_T

Cuts at:      0     173     309 
   Size:        173     136
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     226     309 
   Size:        226      83
 
BglI GCCn_nnn'nGGC

Cuts at:      0     152     309 
   Size:        152     157
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      16     309 
   Size:         16     293
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      13      43     226     256     309 
   Size:         13      30     183      30      53

  Fragments arranged by size: 

                 183      53      30      30      13
 
BseYI C'CCAG_C

Cuts at:      0     108     309 
   Size:        108     201
 
BsrBI CCG'CTC

Cuts at:      0     242     309 
   Size:        242      67
 
BsrFI r'CCGG_y

Cuts at:      0      92     309 
   Size:         92     217
 
BsrGI T'GTAC_A

Cuts at:      0     176     309 
   Size:        176     133
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      48     309 
   Size:         48     261
 
EaeI y'GGCC_r

Cuts at:      0      94     153     309 
   Size:         94      59     156

  Fragments arranged by size: 

                 156      94      59
 
EarI CTCTTCn'nnn_

Cuts at:      0      77     125     309 
   Size:         77      48     184

  Fragments arranged by size: 

                 184      77      48
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     149     309 
   Size:         73      76     160

  Fragments arranged by size: 

                 160      76      73
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     151     183     309 
   Size:        151      32     126

  Fragments arranged by size: 

                 151     126      32
 
NaeI GCC'GGC

Cuts at:      0      94     309 
   Size:         94     215
 
NgoMIV G'CCGG_C

Cuts at:      0      92     309 
   Size:         92     217
 
NspI r_CATG'y

Cuts at:      0      46     177     309 
   Size:         46     131     132

  Fragments arranged by size: 

                 132     131      46
 
PciI A'CATG_T

Cuts at:      0     173     309 
   Size:        173     136
 
SexAI A'CCwGG_T

Cuts at:      0      25     309 
   Size:         25     284
 
SphI G_CATG'C

Cuts at:      0      46     309 
   Size:         46     263
 
TatI w'GTAC_w

Cuts at:      0     176     309 
   Size:        176     133
 

 Enzymes that do cut:

     AcuI   AflIII    BciVI     BglI     BsaI    BsaXI    BseYI    BsrBI
    BsrFI    BsrGI   BstAPI     EaeI     EarI  Eco57MI    Hin4I     NaeI
   NgoMIV     NspI     PciI    SexAI     SphI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrDI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI