(Linear) (Six-base) MAPSORT of: KIEE5102.seq  Check: 9487  from: 1  to: 441



KIEE5102.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:46  ..

AccI GT'mk_AC

Cuts at:      0     104     441 
   Size:        104     337
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     441 
   Size:        294     147
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     217     441 
   Size:        217     224
 
ApaLI G'TGCA_C

Cuts at:      0       4     441 
   Size:          4     437
 
ApoI r'AATT_y

Cuts at:      0     364     441 
   Size:        364      77
 
BamHI G'GATC_C

Cuts at:      0     297     441 
   Size:        297     144
 
BanI G'GyrC_C

Cuts at:      0     208     250     441 
   Size:        208      42     191

  Fragments arranged by size: 

                 208     191      42
 
Bme1580I G_kGCm'C

Cuts at:      0       8     253     441 
   Size:          8     245     188

  Fragments arranged by size: 

                 245     188       8
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     229     441 
   Size:        229     212
 
BsaAI yAC'GTr

Cuts at:      0     294     441 
   Size:        294     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34     441 
   Size:         34     407
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     274     400     441 
   Size:        259      15     126      41

  Fragments arranged by size: 

                 259     126      41      15
 
BsiHKAI G_wGCw'C

Cuts at:      0       8     206     441 
   Size:          8     198     235

  Fragments arranged by size: 

                 235     198       8
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     206     253     441 
   Size:          8     198      47     188

  Fragments arranged by size: 

                 198     188      47       8
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     355     441 
   Size:        355      86
 
BstYI r'GATC_y

Cuts at:      0     141     297     441 
   Size:        141     156     144

  Fragments arranged by size: 

                 156     144     141
 
Bsu36I CC'TnA_GG

Cuts at:      0      17     176     441 
   Size:         17     159     265

  Fragments arranged by size: 

                 265     159      17
 
DraIII CAC_nnn'GTG

Cuts at:      0     268     441 
   Size:        268     173
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     441 
   Size:        294     147
 
EcoO109I rG'GnC_Cy

Cuts at:      0      81     441 
   Size:         81     360
 
EcoRI G'AATT_C

Cuts at:      0     364     441 
   Size:        364      77
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     270     441 
   Size:        270     171
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      42     441 
   Size:         10      32     399

  Fragments arranged by size: 

                 399      32      10
 
PasI CC'CwG_GG

Cuts at:      0      84     441 
   Size:         84     357
 
PmlI CAC'GTG

Cuts at:      0     294     441 
   Size:        294     147
 
SmlI C'TyrA_G

Cuts at:      0     244     441 
   Size:        244     197
 
StyI C'CwwG_G

Cuts at:      0     109     441 
   Size:        109     332
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    ApaLI     ApoI    BamHI     BanI Bme1580I
    BpuEI    BsaAI    BseRI     BsgI  BsiHKAI Bsp1286I    BstXI    BstYI
   Bsu36I   DraIII  Eco57MI EcoO109I    EcoRI     FalI    Hin4I     PasI
     PmlI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI