Restriction map for
pFN21AE5082
(Linear) (Six-base) MAPSORT of: KIEE5082.seq Check: 2916 from: 1 to: 2049
KIEE5082.orf
With 182 enzymes: SgfI *
December 5, 2014 12:59 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 2049
Size: 676 1373
AlwNI CAG_nnn'CTG
Cuts at: 0 1465 2049
Size: 1465 584
ApaI G_GGCC'C
Cuts at: 0 1762 2049
Size: 1762 287
ApoI r'AATT_y
Cuts at: 0 982 1000 1430 1647 1777 2049
Size: 982 18 430 217 130 272
Fragments arranged by size:
982 430 272 217 130 18
AvaI C'yCGr_G
Cuts at: 0 938 2049
Size: 938 1111
BanII G_rGCy'C
Cuts at: 0 400 1506 1762 2049
Size: 400 1106 256 287
Fragments arranged by size:
1106 400 287 256
BbsI GAAGACnn'nnnn_
Cuts at: 0 55 914 1610 2049
Size: 55 859 696 439
Fragments arranged by size:
859 696 439 55
BbvCI CC'TCA_GC
Cuts at: 0 503 2049
Size: 503 1546
BglI GCCn_nnn'nGGC
Cuts at: 0 649 2049
Size: 649 1400
BglII A'GATC_T
Cuts at: 0 299 2049
Size: 299 1750
BlpI GC'TnA_GC
Cuts at: 0 478 1016 2049
Size: 478 538 1033
Fragments arranged by size:
1033 538 478
Bme1580I G_kGCm'C
Cuts at: 0 1762 2049
Size: 1762 287
BmgBI CAC'GTC
Cuts at: 0 1497 2049
Size: 1497 552
BmrI ACTGGGnnnn_n'
Cuts at: 0 2016 2049
Size: 2016 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1063 1262 1397 2049
Size: 403 660 199 135 652
Fragments arranged by size:
660 652 403 199 135
Bpu10I CC'TnA_GC
Cuts at: 0 503 2049
Size: 503 1546
BsaWI w'CCGG_w
Cuts at: 0 1919 2049
Size: 1919 130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 475 1400 1864 2049
Size: 364 111 925 464 185
Fragments arranged by size:
925 464 364 185 111
BseYI C'CCAG_C
Cuts at: 0 644 959 1265 1395 1659 1690 2049
Size: 644 315 306 130 264 31 359
Fragments arranged by size:
644 359 315 306 264 130 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1708 2049
Size: 1708 341
BsiHKAI G_wGCw'C
Cuts at: 0 1506 2049
Size: 1506 543
BsmI GAATG_Cn'
Cuts at: 0 231 2049
Size: 231 1818
BsmBI CGTCTCn'nnnn_
Cuts at: 0 963 2049
Size: 963 1086
Bsp1286I G_dGCh'C
Cuts at: 0 400 1506 1762 2049
Size: 400 1106 256 287
Fragments arranged by size:
1106 400 287 256
BspHI T'CATG_A
Cuts at: 0 584 2049
Size: 584 1465
BsrDI GCAATG_nn'
Cuts at: 0 681 716 947 1115 2049
Size: 681 35 231 168 934
Fragments arranged by size:
934 681 231 168 35
BssSI C'ACGA_G
Cuts at: 0 1970 2049
Size: 1970 79
BstBI TT'CG_AA
Cuts at: 0 1644 2049
Size: 1644 405
BstXI CCAn_nnnn'nTGG
Cuts at: 0 687 2049
Size: 687 1362
BstYI r'GATC_y
Cuts at: 0 299 2049
Size: 299 1750
BtgI C'CryG_G
Cuts at: 0 37 710 2049
Size: 37 673 1339
Fragments arranged by size:
1339 673 37
BtsI GCAGTG_nn'
Cuts at: 0 252 2049
Size: 252 1797
DraI TTT'AAA
Cuts at: 0 981 2049
Size: 981 1068
DrdI GACnn_nn'nnGTC
Cuts at: 0 940 2049
Size: 940 1109
EarI CTCTTCn'nnn_
Cuts at: 0 52 356 859 1529 2049
Size: 52 304 503 670 520
Fragments arranged by size:
670 520 503 304 52
EcoICRI GAG'CTC
Cuts at: 0 1504 2049
Size: 1504 545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 676 1063 1262 1397 2049
Size: 403 273 387 199 135 652
Fragments arranged by size:
652 403 387 273 199 135
EcoO109I rG'GnC_Cy
Cuts at: 0 63 1758 1759 2010 2049
Size: 63 1695 1 251 39
Fragments arranged by size:
1695 251 63 39 1
HaeII r_GCGC'y
Cuts at: 0 966 2049
Size: 966 1083
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 901 933 1020 1052 1395 1427 1837 1869
Size: 901 32 87 32 343 32 410 32
Cuts at: 1869 2049
Size: 180
Fragments arranged by size:
901 410 343 180 87 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1284 1737 2049
Size: 1284 453 312
Fragments arranged by size:
1284 453 312
HindIII A'AGCT_T
Cuts at: 0 1594 2049
Size: 1594 455
MslI CAynn'nnrTG
Cuts at: 0 583 1730 2049
Size: 583 1147 319
Fragments arranged by size:
1147 583 319
MspA1I CmG'CkG
Cuts at: 0 477 619 648 2049
Size: 477 142 29 1401
Fragments arranged by size:
1401 477 142 29
NcoI C'CATG_G
Cuts at: 0 37 710 2049
Size: 37 673 1339
Fragments arranged by size:
1339 673 37
NspI r_CATG'y
Cuts at: 0 408 834 2049
Size: 408 426 1215
Fragments arranged by size:
1215 426 408
PasI CC'CwG_GG
Cuts at: 0 379 2049
Size: 379 1670
PflMI CCAn_nnn'nTGG
Cuts at: 0 711 2049
Size: 711 1338
PfoI T'CCnGG_A
Cuts at: 0 770 2049
Size: 770 1279
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1924 1956 2049
Size: 1924 32 93
Fragments arranged by size:
1924 93 32
PpuMI rG'GwC_Cy
Cuts at: 0 63 2010 2049
Size: 63 1947 39
Fragments arranged by size:
1947 63 39
PspOMI G'GGCC_C
Cuts at: 0 1758 2049
Size: 1758 291
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1625 1657 2049
Size: 1625 32 392
Fragments arranged by size:
1625 392 32
PvuII CAG'CTG
Cuts at: 0 477 619 648 2049
Size: 477 142 29 1401
Fragments arranged by size:
1401 477 142 29
SacI G_AGCT'C
Cuts at: 0 1506 2049
Size: 1506 543
SapI GCTCTTCn'nnn_
Cuts at: 0 356 2049
Size: 356 1693
SfcI C'TryA_G
Cuts at: 0 791 1051 1955 2049
Size: 791 260 904 94
Fragments arranged by size:
904 791 260 94
SpeI A'CTAG_T
Cuts at: 0 1893 2049
Size: 1893 156
SphI G_CATG'C
Cuts at: 0 408 834 2049
Size: 408 426 1215
Fragments arranged by size:
1215 426 408
SspI AAT'ATT
Cuts at: 0 852 2049
Size: 852 1197
StyI C'CwwG_G
Cuts at: 0 37 710 1763 2049
Size: 37 673 1053 286
Fragments arranged by size:
1053 673 286 37
TatI w'GTAC_w
Cuts at: 0 1890 2049
Size: 1890 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 752 784 2049
Size: 752 32 1265
Fragments arranged by size:
1265 752 32
XmnI GAAnn'nnTTC
Cuts at: 0 539 1466 2049
Size: 539 927 583
Fragments arranged by size:
927 583 539
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AvaI BanII BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BsrDI BssSI BstBI BstXI BstYI BtgI BtsI
DraI DrdI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII HindIII MslI MspA1I NcoI NspI PasI PflMI
PfoI PpiI PpuMI PspOMI PsrI PvuII SacI SapI
SfcI SpeI SphI SspI StyI TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BsiEI BsiWI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII EaeI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsiI PspXI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SrfI StuI SwaI TaqII Tth111I XbaI
XcmI XhoI XmaI ZraI