Restriction map for
pFN21AE5080
(Linear) (Six-base) MAPSORT of: KIEE5080.seq Check: 8692 from: 1 to: 963
KIEE5080.orf
With 182 enzymes: SgfI *
December 5, 2014 12:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 963
Size: 721 242
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 274 963
Size: 46 228 689
Fragments arranged by size:
689 228 46
AlwNI CAG_nnn'CTG
Cuts at: 0 546 963
Size: 546 417
ApoI r'AATT_y
Cuts at: 0 243 568 963
Size: 243 325 395
Fragments arranged by size:
395 325 243
AseI AT'TA_AT
Cuts at: 0 575 963
Size: 575 388
BamHI G'GATC_C
Cuts at: 0 402 963
Size: 402 561
BanII G_rGCy'C
Cuts at: 0 464 963
Size: 464 499
BbsI GAAGACnn'nnnn_
Cuts at: 0 696 963
Size: 696 267
BbvCI CC'TCA_GC
Cuts at: 0 686 963
Size: 686 277
BfrBI ATG'CAT
Cuts at: 0 76 468 963
Size: 76 392 495
Fragments arranged by size:
495 392 76
BmrI ACTGGGnnnn_n'
Cuts at: 0 225 963
Size: 225 738
Bpu10I CC'TnA_GC
Cuts at: 0 686 963
Size: 686 277
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 963
Size: 112 851
BseYI C'CCAG_C
Cuts at: 0 445 963
Size: 445 518
BsiHKAI G_wGCw'C
Cuts at: 0 641 963
Size: 641 322
BsmI GAATG_Cn'
Cuts at: 0 284 595 963
Size: 284 311 368
Fragments arranged by size:
368 311 284
BsmBI CGTCTCn'nnnn_
Cuts at: 0 160 963
Size: 160 803
Bsp1286I G_dGCh'C
Cuts at: 0 464 641 963
Size: 464 177 322
Fragments arranged by size:
464 322 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 721 963
Size: 107 614 242
Fragments arranged by size:
614 242 107
BsrDI GCAATG_nn'
Cuts at: 0 236 963
Size: 236 727
BstAPI GCAn_nnn'nTGC
Cuts at: 0 592 963
Size: 592 371
BstYI r'GATC_y
Cuts at: 0 402 963
Size: 402 561
BtgI C'CryG_G
Cuts at: 0 143 963
Size: 143 820
BtsI GCAGTG_nn'
Cuts at: 0 431 963
Size: 431 532
DraI TTT'AAA
Cuts at: 0 182 963
Size: 182 781
EaeI y'GGCC_r
Cuts at: 0 329 963
Size: 329 634
EarI CTCTTCn'nnn_
Cuts at: 0 200 499 911 963
Size: 200 299 412 52
Fragments arranged by size:
412 299 200 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 274 963
Size: 46 228 689
Fragments arranged by size:
689 228 46
EcoO109I rG'GnC_Cy
Cuts at: 0 450 963
Size: 450 513
EcoRI G'AATT_C
Cuts at: 0 568 963
Size: 568 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 778 810 963
Size: 778 32 153
Fragments arranged by size:
778 153 32
HincII GTy'rAC
Cuts at: 0 376 963
Size: 376 587
HindIII A'AGCT_T
Cuts at: 0 58 963
Size: 58 905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 963
Size: 865 98
MscI TGG'CCA
Cuts at: 0 331 963
Size: 331 632
MslI CAynn'nnrTG
Cuts at: 0 142 343 963
Size: 142 201 620
Fragments arranged by size:
620 201 142
MspA1I CmG'CkG
Cuts at: 0 685 754 963
Size: 685 69 209
Fragments arranged by size:
685 209 69
NsiI A_TGCA'T
Cuts at: 0 78 470 963
Size: 78 392 493
Fragments arranged by size:
493 392 78
NspI r_CATG'y
Cuts at: 0 76 424 472 963
Size: 76 348 48 491
Fragments arranged by size:
491 348 76 48
PshAI GACnn'nnGTC
Cuts at: 0 152 700 963
Size: 152 548 263
Fragments arranged by size:
548 263 152
PstI C_TGCA'G
Cuts at: 0 612 759 963
Size: 612 147 204
Fragments arranged by size:
612 204 147
PvuII CAG'CTG
Cuts at: 0 685 754 963
Size: 685 69 209
Fragments arranged by size:
685 209 69
SapI GCTCTTCn'nnn_
Cuts at: 0 911 963
Size: 911 52
SfcI C'TryA_G
Cuts at: 0 414 608 662 755 963
Size: 414 194 54 93 208
Fragments arranged by size:
414 208 194 93 54
SphI G_CATG'C
Cuts at: 0 424 472 963
Size: 424 48 491
Fragments arranged by size:
491 424 48
StyI C'CwwG_G
Cuts at: 0 698 963
Size: 698 265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 963
Size: 141 822
XmnI GAAnn'nnTTC
Cuts at: 0 920 963
Size: 920 43
Enzymes that do cut:
AarI AcuI AlwNI ApoI AseI BamHI BanII BbsI
BbvCI BfrBI BmrI Bpu10I BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrDI BstAPI BstYI BtgI BtsI
DraI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I HincII
HindIII MmeI MscI MslI MspA1I NsiI NspI PshAI
PstI PvuII SapI SfcI SphI StyI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BanI BbeI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI
BsiEI BsiWI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI ZraI