Restriction map for
pFN21AE5077
(Linear) (Six-base) MAPSORT of: KIEE5077.seq Check: 1904 from: 1 to: 597
KIEE5077.orf
With 182 enzymes: SgfI *
September 8, 2014 17:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 597
Size: 183 414
AflII C'TTAA_G
Cuts at: 0 385 597
Size: 385 212
AlwNI CAG_nnn'CTG
Cuts at: 0 206 597
Size: 206 391
ApaLI G'TGCA_C
Cuts at: 0 106 597
Size: 106 491
AvaI C'yCGr_G
Cuts at: 0 66 562 597
Size: 66 496 35
Fragments arranged by size:
496 66 35
AvrII C'CTAG_G
Cuts at: 0 516 597
Size: 516 81
BamHI G'GATC_C
Cuts at: 0 432 597
Size: 432 165
BanI G'GyrC_C
Cuts at: 0 33 93 597
Size: 33 60 504
Fragments arranged by size:
504 60 33
BanII G_rGCy'C
Cuts at: 0 73 597
Size: 73 524
BbeI G_GCGC'C
Cuts at: 0 37 97 597
Size: 37 60 500
Fragments arranged by size:
500 60 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 189 597
Size: 189 408
BglI GCCn_nnn'nGGC
Cuts at: 0 169 266 597
Size: 169 97 331
Fragments arranged by size:
331 169 97
Bme1580I G_kGCm'C
Cuts at: 0 110 398 597
Size: 110 288 199
Fragments arranged by size:
288 199 110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 554 597
Size: 554 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 597
Size: 235 362
BsaAI yAC'GTr
Cuts at: 0 236 597
Size: 236 361
BsaHI Gr'CG_yC
Cuts at: 0 34 94 352 597
Size: 34 60 258 245
Fragments arranged by size:
258 245 60 34
BsaWI w'CCGG_w
Cuts at: 0 137 296 597
Size: 137 159 301
Fragments arranged by size:
301 159 137
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 525 597
Size: 525 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 597
Size: 256 341
BsiEI CG_ry'CG
Cuts at: 0 213 368 597
Size: 213 155 229
Fragments arranged by size:
229 213 155
BsiHKAI G_wGCw'C
Cuts at: 0 110 597
Size: 110 487
BsiWI C'GTAC_G
Cuts at: 0 303 597
Size: 303 294
Bsp1286I G_dGCh'C
Cuts at: 0 73 110 398 597
Size: 73 37 288 199
Fragments arranged by size:
288 199 73 37
BspEI T'CCGG_A
Cuts at: 0 137 597
Size: 137 460
BsrFI r'CCGG_y
Cuts at: 0 363 597
Size: 363 234
BssHII G'CGCG_C
Cuts at: 0 186 597
Size: 186 411
BstYI r'GATC_y
Cuts at: 0 134 245 432 597
Size: 134 111 187 165
Fragments arranged by size:
187 165 134 111
EaeI y'GGCC_r
Cuts at: 0 210 365 597
Size: 210 155 232
Fragments arranged by size:
232 210 155
EagI C'GGCC_G
Cuts at: 0 210 365 597
Size: 210 155 232
Fragments arranged by size:
232 210 155
EarI CTCTTCn'nnn_
Cuts at: 0 23 597
Size: 23 574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 554 597
Size: 183 371 43
Fragments arranged by size:
371 183 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 274 597
Size: 274 323
HaeII r_GCGC'y
Cuts at: 0 37 97 258 597
Size: 37 60 161 339
Fragments arranged by size:
339 161 60 37
KasI G'GCGC_C
Cuts at: 0 33 93 597
Size: 33 60 504
Fragments arranged by size:
504 60 33
MspA1I CmG'CkG
Cuts at: 0 40 203 532 597
Size: 40 163 329 65
Fragments arranged by size:
329 163 65 40
NaeI GCC'GGC
Cuts at: 0 365 597
Size: 365 232
NarI GG'CG_CC
Cuts at: 0 34 94 597
Size: 34 60 503
Fragments arranged by size:
503 60 34
NgoMIV G'CCGG_C
Cuts at: 0 363 597
Size: 363 234
NspI r_CATG'y
Cuts at: 0 114 597
Size: 114 483
PmlI CAC'GTG
Cuts at: 0 236 597
Size: 236 361
PvuII CAG'CTG
Cuts at: 0 532 597
Size: 532 65
SexAI A'CCwGG_T
Cuts at: 0 275 597
Size: 275 322
SfoI GGC'GCC
Cuts at: 0 35 95 597
Size: 35 60 502
Fragments arranged by size:
502 60 35
SmaI CCC'GGG
Cuts at: 0 68 597
Size: 68 529
SmlI C'TyrA_G
Cuts at: 0 250 385 597
Size: 250 135 212
Fragments arranged by size:
250 212 135
SrfI GCCC'GGGC
Cuts at: 0 68 597
Size: 68 529
StyI C'CwwG_G
Cuts at: 0 419 516 597
Size: 419 97 81
Fragments arranged by size:
419 97 81
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 165 426 597
Size: 165 261 171
Fragments arranged by size:
261 171 165
XmaI C'CCGG_G
Cuts at: 0 66 597
Size: 66 531
Enzymes that do cut:
AcuI AflII AlwNI ApaLI AvaI AvrII BamHI BanI
BanII BbeI BbsI BglI Bme1580I BpmI BpuEI BsaAI
BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspEI BsrFI BssHII BstYI EaeI EagI EarI Eco57MI
EcoNI HaeII KasI MspA1I NaeI NarI NgoMIV NspI
PmlI PvuII SexAI SfoI SmaI SmlI SrfI StyI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApoI AscI AseI
AsiSI BaeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI
BsaXI BseYI BsmI BsmBI BspHI BspMI BsrBI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmnI ZraI