Restriction map for
pFN21AE5074
(Linear) (Six-base) MAPSORT of: KIEE5074.seq Check: 6386 from: 1 to: 528
KIEE5074.orf
With 182 enzymes: SgfI *
December 5, 2014 12:59 ..
AccI GT'mk_AC
Cuts at: 0 302 528
Size: 302 226
AfeI AGC'GCT
Cuts at: 0 405 528
Size: 405 123
AlwNI CAG_nnn'CTG
Cuts at: 0 16 528
Size: 16 512
ApaI G_GGCC'C
Cuts at: 0 520 528
Size: 520 8
AvaI C'yCGr_G
Cuts at: 0 82 429 528
Size: 82 347 99
Fragments arranged by size:
347 99 82
BanI G'GyrC_C
Cuts at: 0 20 289 359 528
Size: 20 269 70 169
Fragments arranged by size:
269 169 70 20
BanII G_rGCy'C
Cuts at: 0 251 500 520 528
Size: 251 249 20 8
Fragments arranged by size:
251 249 20 8
BbeI G_GCGC'C
Cuts at: 0 24 293 528
Size: 24 269 235
Fragments arranged by size:
269 235 24
BbvCI CC'TCA_GC
Cuts at: 0 224 370 528
Size: 224 146 158
Fragments arranged by size:
224 158 146
BglI GCCn_nnn'nGGC
Cuts at: 0 268 472 528
Size: 268 204 56
Fragments arranged by size:
268 204 56
Bme1580I G_kGCm'C
Cuts at: 0 206 520 528
Size: 206 314 8
Fragments arranged by size:
314 206 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 471 528
Size: 471 57
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 433 454 528
Size: 265 168 21 74
Fragments arranged by size:
265 168 74 21
Bpu10I CC'TnA_GC
Cuts at: 0 224 370 528
Size: 224 146 158
Fragments arranged by size:
224 158 146
BsaI GGTCTCn'nnnn_
Cuts at: 0 446 528
Size: 446 82
BsaAI yAC'GTr
Cuts at: 0 254 528
Size: 254 274
BsaHI Gr'CG_yC
Cuts at: 0 21 290 528
Size: 21 269 238
Fragments arranged by size:
269 238 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 238 268 528
Size: 238 30 260
Fragments arranged by size:
260 238 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 475 528
Size: 124 351 53
Fragments arranged by size:
351 124 53
BseYI C'CCAG_C
Cuts at: 0 16 501 528
Size: 16 485 27
Fragments arranged by size:
485 27 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 528
Size: 324 204
BsiEI CG_ry'CG
Cuts at: 0 318 332 528
Size: 318 14 196
Fragments arranged by size:
318 196 14
BsmBI CGTCTCn'nnnn_
Cuts at: 0 156 528
Size: 156 372
Bsp1286I G_dGCh'C
Cuts at: 0 206 251 500 520 528
Size: 206 45 249 20 8
Fragments arranged by size:
249 206 45 20 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 528
Size: 239 289
BsrBI CCG'CTC
Cuts at: 0 326 528
Size: 326 202
BsrFI r'CCGG_y
Cuts at: 0 6 283 528
Size: 6 277 245
Fragments arranged by size:
277 245 6
BssHII G'CGCG_C
Cuts at: 0 89 121 123 174 176 279 528
Size: 89 32 2 51 2 103 249
Fragments arranged by size:
249 103 89 51 32 2 2
Bsu36I CC'TnA_GG
Cuts at: 0 507 521 528
Size: 507 14 7
Fragments arranged by size:
507 14 7
BtgI C'CryG_G
Cuts at: 0 24 98 263 272 363 528
Size: 24 74 165 9 91 165
Fragments arranged by size:
165 165 91 74 24 9
DraIII CAC_nnn'GTG
Cuts at: 0 140 528
Size: 140 388
EaeI y'GGCC_r
Cuts at: 0 315 329 528
Size: 315 14 199
Fragments arranged by size:
315 199 14
EagI C'GGCC_G
Cuts at: 0 315 329 528
Size: 315 14 199
Fragments arranged by size:
315 199 14
EarI CTCTTCn'nnn_
Cuts at: 0 82 394 528
Size: 82 312 134
Fragments arranged by size:
312 134 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 433 454 528
Size: 265 168 21 74
Fragments arranged by size:
265 168 74 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 379 528
Size: 379 149
EcoO109I rG'GnC_Cy
Cuts at: 0 228 465 517 528
Size: 228 237 52 11
Fragments arranged by size:
237 228 52 11
HaeII r_GCGC'y
Cuts at: 0 24 293 407 528
Size: 24 269 114 121
Fragments arranged by size:
269 121 114 24
HincII GTy'rAC
Cuts at: 0 147 528
Size: 147 381
KasI G'GCGC_C
Cuts at: 0 20 289 528
Size: 20 269 239
Fragments arranged by size:
269 239 20
MspA1I CmG'CkG
Cuts at: 0 26 100 117 274 365 528
Size: 26 74 17 157 91 163
Fragments arranged by size:
163 157 91 74 26 17
NaeI GCC'GGC
Cuts at: 0 285 528
Size: 285 243
NarI GG'CG_CC
Cuts at: 0 21 290 528
Size: 21 269 238
Fragments arranged by size:
269 238 21
NcoI C'CATG_G
Cuts at: 0 263 528
Size: 263 265
NgoMIV G'CCGG_C
Cuts at: 0 283 528
Size: 283 245
PmlI CAC'GTG
Cuts at: 0 254 528
Size: 254 274
PpuMI rG'GwC_Cy
Cuts at: 0 228 528
Size: 228 300
PspOMI G'GGCC_C
Cuts at: 0 516 528
Size: 516 12
PstI C_TGCA'G
Cuts at: 0 491 528
Size: 491 37
PvuII CAG'CTG
Cuts at: 0 117 528
Size: 117 411
SacII CC_GC'GG
Cuts at: 0 27 101 275 366 528
Size: 27 74 174 91 162
Fragments arranged by size:
174 162 91 74 27
SapI GCTCTTCn'nnn_
Cuts at: 0 394 528
Size: 394 134
SbfI CC_TGCA'GG
Cuts at: 0 491 528
Size: 491 37
SfcI C'TryA_G
Cuts at: 0 487 528
Size: 487 41
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 472 528
Size: 472 56
SfoI GGC'GCC
Cuts at: 0 22 291 528
Size: 22 269 237
Fragments arranged by size:
269 237 22
SgrAI Cr'CCGG_yG
Cuts at: 0 283 528
Size: 283 245
SmaI CCC'GGG
Cuts at: 0 431 528
Size: 431 97
StuI AGG'CCT
Cuts at: 0 385 528
Size: 385 143
StyI C'CwwG_G
Cuts at: 0 263 528
Size: 263 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 238 270 528
Size: 238 32 258
Fragments arranged by size:
258 238 32
XmaI C'CCGG_G
Cuts at: 0 429 528
Size: 429 99
XmnI GAAnn'nnTTC
Cuts at: 0 385 528
Size: 385 143
Enzymes that do cut:
AccI AfeI AlwNI ApaI AvaI BanI BanII BbeI
BbvCI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssHII Bsu36I BtgI DraIII EaeI
EagI EarI Eco57MI EcoNI EcoO109I HaeII HincII KasI
MspA1I NaeI NarI NcoI NgoMIV PmlI PpuMI PspOMI
PstI PvuII SacII SapI SbfI SfcI SfiI SfoI
SgrAI SmaI StuI StyI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaBI
BsaWI BsiHKAI BsiWI BsmI BspEI BspHI BsrDI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI
BtsI ClaI CspCI DraI DrdI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SalI SanDI ScaI SexAI SgfI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI ZraI