(Linear) (Six-base) MAPSORT of: KIEE5072.seq  Check: 783  from: 1  to: 450



KIEE5072.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      90     450 
   Size:         90     360
 
ApaI G_GGCC'C

Cuts at:      0     140     450 
   Size:        140     310
 
ApaLI G'TGCA_C

Cuts at:      0     277     450 
   Size:        277     173
 
BanI G'GyrC_C

Cuts at:      0     110     307     450 
   Size:        110     197     143

  Fragments arranged by size: 

                 197     143     110
 
BanII G_rGCy'C

Cuts at:      0     140     450 
   Size:        140     310
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     417     450 
   Size:        417      33
 
BglI GCCn_nnn'nGGC

Cuts at:      0     305     450 
   Size:        305     145
 
Bme1580I G_kGCm'C

Cuts at:      0     140     281     450 
   Size:        140     141     169

  Fragments arranged by size: 

                 169     141     140
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     394     450 
   Size:        394      56
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     254     284     450 
   Size:        254      30     166

  Fragments arranged by size: 

                 254     166      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     157     253     450 
   Size:         28     129      96     197

  Fragments arranged by size: 

                 197     129      96      28
 
BsiEI CG_ry'CG

Cuts at:      0     225     450 
   Size:        225     225
 
BsiHKAI G_wGCw'C

Cuts at:      0     281     288     450 
   Size:        281       7     162

  Fragments arranged by size: 

                 281     162       7
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     281     288     450 
   Size:        140     141       7     162

  Fragments arranged by size: 

                 162     141     140       7
 
BsrBI CCG'CTC

Cuts at:      0     339     450 
   Size:        339     111
 
BsrDI GCAATG_nn'

Cuts at:      0     362     450 
   Size:        362      88
 
BsrFI r'CCGG_y

Cuts at:      0      41     204     450 
   Size:         41     163     246

  Fragments arranged by size: 

                 246     163      41
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     180     358     450 
   Size:        180     178      92

  Fragments arranged by size: 

                 180     178      92
 
BtgI C'CryG_G

Cuts at:      0      64     450 
   Size:         64     386
 
DraIII CAC_nnn'GTG

Cuts at:      0     117     450 
   Size:        117     333
 
EaeI y'GGCC_r

Cuts at:      0     297     450 
   Size:        297     153
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     331     450 
   Size:        331     119
 
EcoO109I rG'GnC_Cy

Cuts at:      0     137     450 
   Size:        137     313
 
FspI TGC'GCA

Cuts at:      0     184     450 
   Size:        184     266
 
HaeII r_GCGC'y

Cuts at:      0      61     450 
   Size:         61     389
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      74     399     450 
   Size:         74     325      51

  Fragments arranged by size: 

                 325      74      51
 
MspA1I CmG'CkG

Cuts at:      0      66     155     450 
   Size:         66      89     295

  Fragments arranged by size: 

                 295      89      66
 
NaeI GCC'GGC

Cuts at:      0      43     206     450 
   Size:         43     163     244

  Fragments arranged by size: 

                 244     163      43
 
NgoMIV G'CCGG_C

Cuts at:      0      41     204     450 
   Size:         41     163     246

  Fragments arranged by size: 

                 246     163      41
 
PspOMI G'GGCC_C

Cuts at:      0     136     450 
   Size:        136     314
 
PstI C_TGCA'G

Cuts at:      0     354     450 
   Size:        354      96
 
PvuII CAG'CTG

Cuts at:      0     155     450 
   Size:        155     295
 
SacII CC_GC'GG

Cuts at:      0      67     450 
   Size:         67     383
 
SexAI A'CCwGG_T

Cuts at:      0     113     326     450 
   Size:        113     213     124

  Fragments arranged by size: 

                 213     124     113
 
SfcI C'TryA_G

Cuts at:      0     350     450 
   Size:        350     100
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      18     450 
   Size:         18     432
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     103     135     254     286     450 
   Size:        103      32     119      32     164

  Fragments arranged by size: 

                 164     119     103      32      32
 

 Enzymes that do cut:

    AlwNI     ApaI    ApaLI     BanI    BanII     BbsI     BglI Bme1580I
     BsaI    BsaXI     BsgI    BsiEI  BsiHKAI Bsp1286I    BsrBI    BsrDI
    BsrFI   BstAPI     BtgI   DraIII     EaeI    EcoNI EcoO109I     FspI
    HaeII     MmeI   MspA1I     NaeI   NgoMIV   PspOMI     PstI    PvuII
    SacII    SexAI     SfcI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI
     FseI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI