Restriction map for
pFN21AE5066
(Linear) (Six-base) MAPSORT of: KIEE5066.seq Check: 8479 from: 1 to: 2904
KIEE5066.orf
With 182 enzymes: SgfI *
December 5, 2014 12:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2189 2754 2904
Size: 2189 565 150
Fragments arranged by size:
2189 565 150
AccI GT'mk_AC
Cuts at: 0 1307 2904
Size: 1307 1597
AclI AA'CG_TT
Cuts at: 0 2831 2904
Size: 2831 73
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1307 1976 2904
Size: 1111 196 669 928
Fragments arranged by size:
1111 928 669 196
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1525 1557 2904
Size: 1525 32 1347
Fragments arranged by size:
1525 1347 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1068 1197 2904
Size: 1068 129 1707
Fragments arranged by size:
1707 1068 129
ApaI G_GGCC'C
Cuts at: 0 547 2904
Size: 547 2357
ApoI r'AATT_y
Cuts at: 0 471 1187 2904
Size: 471 716 1717
Fragments arranged by size:
1717 716 471
AseI AT'TA_AT
Cuts at: 0 1124 2904
Size: 1124 1780
AvaI C'yCGr_G
Cuts at: 0 2190 2887 2904
Size: 2190 697 17
Fragments arranged by size:
2190 697 17
BanI G'GyrC_C
Cuts at: 0 823 2269 2593 2904
Size: 823 1446 324 311
Fragments arranged by size:
1446 823 324 311
BanII G_rGCy'C
Cuts at: 0 307 547 1418 1787 2904
Size: 307 240 871 369 1117
Fragments arranged by size:
1117 871 369 307 240
BbeI G_GCGC'C
Cuts at: 0 2597 2904
Size: 2597 307
BbsI GAAGACnn'nnnn_
Cuts at: 0 1385 1800 2536 2904
Size: 1385 415 736 368
Fragments arranged by size:
1385 736 415 368
BbvCI CC'TCA_GC
Cuts at: 0 854 2598 2904
Size: 854 1744 306
Fragments arranged by size:
1744 854 306
BciVI GTATCCnnnnn_n'
Cuts at: 0 2254 2904
Size: 2254 650
BfrBI ATG'CAT
Cuts at: 0 1078 2904
Size: 1078 1826
BglI GCCn_nnn'nGGC
Cuts at: 0 2284 2904
Size: 2284 620
BglII A'GATC_T
Cuts at: 0 444 461 638 1622 2904
Size: 444 17 177 984 1282
Fragments arranged by size:
1282 984 444 177 17
BlpI GC'TnA_GC
Cuts at: 0 196 1607 2904
Size: 196 1411 1297
Fragments arranged by size:
1411 1297 196
Bme1580I G_kGCm'C
Cuts at: 0 547 820 828 1425 2904
Size: 547 273 8 597 1479
Fragments arranged by size:
1479 597 547 273 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 848 2418 2904
Size: 848 1570 486
Fragments arranged by size:
1570 848 486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1453 1461 2599 2904
Size: 655 798 8 1138 305
Fragments arranged by size:
1138 798 655 305 8
Bpu10I CC'TnA_GC
Cuts at: 0 854 1085 1697 2598 2904
Size: 854 231 612 901 306
Fragments arranged by size:
901 854 612 306 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 2904
Size: 1132 1772
BsaI GGTCTCn'nnnn_
Cuts at: 0 2056 2904
Size: 2056 848
BsaAI yAC'GTr
Cuts at: 0 71 2699 2904
Size: 71 2628 205
Fragments arranged by size:
2628 205 71
BsaBI GATnn'nnATC
Cuts at: 0 275 2904
Size: 275 2629
BsaHI Gr'CG_yC
Cuts at: 0 596 1167 2594 2904
Size: 596 571 1427 310
Fragments arranged by size:
1427 596 571 310
BsaWI w'CCGG_w
Cuts at: 0 578 2246 2904
Size: 578 1668 658
Fragments arranged by size:
1668 658 578
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 965 995 1525 1555 1700 1730 2195 2225
Size: 965 30 530 30 145 30 465 30
Cuts at: 2225 2904
Size: 679
Fragments arranged by size:
965 679 530 465 145 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 352 1228 1342 1721 2904
Size: 352 876 114 379 1183
Fragments arranged by size:
1183 876 379 352 114
BseYI C'CCAG_C
Cuts at: 0 232 300 1343 2876 2904
Size: 232 68 1043 1533 28
Fragments arranged by size:
1533 1043 232 68 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 2392 2779 2795 2904
Size: 1159 1233 387 16 109
Fragments arranged by size:
1233 1159 387 109 16
BsiEI CG_ry'CG
Cuts at: 0 579 2904
Size: 579 2325
BsiHKAI G_wGCw'C
Cuts at: 0 1418 2904
Size: 1418 1486
BsmI GAATG_Cn'
Cuts at: 0 380 457 683 1698 2727 2904
Size: 380 77 226 1015 1029 177
Fragments arranged by size:
1029 1015 380 226 177 77
BsmBI CGTCTCn'nnnn_
Cuts at: 0 966 2094 2904
Size: 966 1128 810
Fragments arranged by size:
1128 966 810
Bsp1286I G_dGCh'C
Cuts at: 0 307 547 820 828 1418 1425 1787 2904
Size: 307 240 273 8 590 7 362 1117
Fragments arranged by size:
1117 590 362 307 273 240 8 7
BspEI T'CCGG_A
Cuts at: 0 2246 2904
Size: 2246 658
BspHI T'CATG_A
Cuts at: 0 1802 2904
Size: 1802 1102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2189 2754 2904
Size: 2189 565 150
Fragments arranged by size:
2189 565 150
BsrBI CCG'CTC
Cuts at: 0 2591 2904
Size: 2591 313
BsrDI GCAATG_nn'
Cuts at: 0 2231 2904
Size: 2231 673
BsrFI r'CCGG_y
Cuts at: 0 938 2904
Size: 938 1966
BstEII G'GTnAC_C
Cuts at: 0 1741 2904
Size: 1741 1163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 99 376 918 1573 2904
Size: 99 277 542 655 1331
Fragments arranged by size:
1331 655 542 277 99
BstYI r'GATC_y
Cuts at: 0 444 461 566 638 1266 1622 2904
Size: 444 17 105 72 628 356 1282
Fragments arranged by size:
1282 628 444 356 105 72 17
Bsu36I CC'TnA_GG
Cuts at: 0 2290 2904
Size: 2290 614
BtgI C'CryG_G
Cuts at: 0 185 2904
Size: 185 2719
BtsI GCAGTG_nn'
Cuts at: 0 567 1226 1668 2408 2904
Size: 567 659 442 740 496
Fragments arranged by size:
740 659 567 496 442
DrdI GACnn_nn'nnGTC
Cuts at: 0 787 2904
Size: 787 2117
EaeI y'GGCC_r
Cuts at: 0 936 2405 2861 2904
Size: 936 1469 456 43
Fragments arranged by size:
1469 936 456 43
EarI CTCTTCn'nnn_
Cuts at: 0 701 1127 1283 1319 1406 1544 1952 2635
Size: 701 426 156 36 87 138 408 683
Cuts at: 2635 2671 2904
Size: 36 233
Fragments arranged by size:
701 683 426 408 233 156 138 87
36 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 973 2904
Size: 973 1931
EcoICRI GAG'CTC
Cuts at: 0 1416 2904
Size: 1416 1488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1111 1307 1453 1461 1976 2599 2904
Size: 655 456 196 146 8 515 623 305
Fragments arranged by size:
655 623 515 456 305 196 146 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1645 2395 2467 2845 2904
Size: 1645 750 72 378 59
Fragments arranged by size:
1645 750 378 72 59
EcoO109I rG'GnC_Cy
Cuts at: 0 544 1098 1296 2040 2100 2286 2400 2607
Size: 544 554 198 744 60 186 114 207
Cuts at: 2607 2904
Size: 297
Fragments arranged by size:
744 554 544 297 207 198 186 114
60
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 537 2904
Size: 537 2367
FseI GG_CCGG'CC
Cuts at: 0 942 2904
Size: 942 1962
FspI TGC'GCA
Cuts at: 0 1528 2904
Size: 1528 1376
HaeII r_GCGC'y
Cuts at: 0 2597 2854 2904
Size: 2597 257 50
Fragments arranged by size:
2597 257 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 573 605 965 997 1607 1639 2113 2145
Size: 573 32 360 32 610 32 474 32
Cuts at: 2145 2904
Size: 759
Fragments arranged by size:
759 610 573 474 360 32 32 32
32
HincII GTy'rAC
Cuts at: 0 612 2904
Size: 612 2292
KasI G'GCGC_C
Cuts at: 0 2593 2904
Size: 2593 311
MfeI C'AATT_G
Cuts at: 0 167 2904
Size: 167 2737
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 791 2904
Size: 791 2113
MscI TGG'CCA
Cuts at: 0 2407 2863 2904
Size: 2407 456 41
Fragments arranged by size:
2407 456 41
MslI CAynn'nnrTG
Cuts at: 0 71 721 916 2904
Size: 71 650 195 1988
Fragments arranged by size:
1988 650 195 71
MspA1I CmG'CkG
Cuts at: 0 430 1347 2859 2880 2904
Size: 430 917 1512 21 24
Fragments arranged by size:
1512 917 430 24 21
NaeI GCC'GGC
Cuts at: 0 940 2904
Size: 940 1964
NarI GG'CG_CC
Cuts at: 0 2594 2904
Size: 2594 310
NcoI C'CATG_G
Cuts at: 0 185 2904
Size: 185 2719
NgoMIV G'CCGG_C
Cuts at: 0 938 2904
Size: 938 1966
NsiI A_TGCA'T
Cuts at: 0 1080 2904
Size: 1080 1824
NspI r_CATG'y
Cuts at: 0 344 2904
Size: 344 2560
PasI CC'CwG_GG
Cuts at: 0 1068 2904
Size: 1068 1836
PflMI CCAn_nnn'nTGG
Cuts at: 0 838 1068 2753 2904
Size: 838 230 1685 151
Fragments arranged by size:
1685 838 230 151
PfoI T'CCnGG_A
Cuts at: 0 1175 1218 2576 2904
Size: 1175 43 1358 328
Fragments arranged by size:
1358 1175 328 43
PmlI CAC'GTG
Cuts at: 0 2699 2904
Size: 2699 205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1525 1557 2904
Size: 1525 32 1347
Fragments arranged by size:
1525 1347 32
PpuMI rG'GwC_Cy
Cuts at: 0 1098 2100 2904
Size: 1098 1002 804
Fragments arranged by size:
1098 1002 804
PspOMI G'GGCC_C
Cuts at: 0 543 2904
Size: 543 2361
PspXI vC'TCGA_Gb
Cuts at: 0 2190 2904
Size: 2190 714
PstI C_TGCA'G
Cuts at: 0 755 2415 2846 2858 2904
Size: 755 1660 431 12 46
Fragments arranged by size:
1660 755 431 46 12
PvuII CAG'CTG
Cuts at: 0 430 1347 2859 2880 2904
Size: 430 917 1512 21 24
Fragments arranged by size:
1512 917 430 24 21
SacI G_AGCT'C
Cuts at: 0 1418 2904
Size: 1418 1486
SanDI GG'GwC_CC
Cuts at: 0 2100 2904
Size: 2100 804
SapI GCTCTTCn'nnn_
Cuts at: 0 1406 2635 2671 2904
Size: 1406 1229 36 233
Fragments arranged by size:
1406 1229 233 36
SbfI CC_TGCA'GG
Cuts at: 0 2846 2904
Size: 2846 58
SexAI A'CCwGG_T
Cuts at: 0 1640 2904
Size: 1640 1264
SfcI C'TryA_G
Cuts at: 0 751 2411 2842 2854 2904
Size: 751 1660 431 12 50
Fragments arranged by size:
1660 751 431 50 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2284 2904
Size: 2284 620
SfoI GGC'GCC
Cuts at: 0 2595 2904
Size: 2595 309
SmlI C'TyrA_G
Cuts at: 0 1147 2190 2904
Size: 1147 1043 714
Fragments arranged by size:
1147 1043 714
SnaBI TAC'GTA
Cuts at: 0 71 2904
Size: 71 2833
StyI C'CwwG_G
Cuts at: 0 185 1919 1988 2237 2391 2603 2904
Size: 185 1734 69 249 154 212 301
Fragments arranged by size:
1734 301 249 212 185 154 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 382 523 1951 2504 2745 2904
Size: 382 141 1428 553 241 159
Fragments arranged by size:
1428 553 382 241 159 141
TatI w'GTAC_w
Cuts at: 0 1457 2904
Size: 1457 1447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 834 866 1700 1732 2195 2227 2904
Size: 834 32 834 32 463 32 677
Fragments arranged by size:
834 834 677 463 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 96 2904
Size: 96 2808
XhoI C'TCGA_G
Cuts at: 0 2190 2904
Size: 2190 714
XmnI GAAnn'nnTTC
Cuts at: 0 142 806 1540 2539 2629 2904
Size: 142 664 734 999 90 275
Fragments arranged by size:
999 734 664 275 142 90
Enzymes that do cut:
AarI AccI AclI AcuI AloI AlwNI ApaI ApoI
AseI AvaI BanI BanII BbeI BbsI BbvCI BciVI
BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI
Bsu36I BtgI BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I
HincII KasI MfeI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SmlI
SnaBI StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI
AlfI ApaLI AscI AsiSI AvrII BaeI BamHI BcgI
BclI BmgBI BmtI BplI BsiWI BsrGI BssHII BssSI
BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII
EagI EcoRI EcoRV FspAI HindIII HpaI KpnI MluI
NdeI NheI NotI NruI PacI PciI PmeI PshAI
PsiI PsrI PvuI RsrII SacII SalI ScaI SgfI
SgrAI SmaI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XmaI ZraI