Restriction map for
pFN21AE5064
(Linear) (Six-base) MAPSORT of: KIEE5064.seq Check: 4502 from: 1 to: 1662
KIEE5064.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
Acc65I G'GTAC_C
Cuts at: 0 18 1662
Size: 18 1644
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 384 1139 1388 1662
Size: 279 105 755 249 274
Fragments arranged by size:
755 279 274 249 105
AflIII A'CryG_T
Cuts at: 0 1430 1662
Size: 1430 232
AhdI GACnn_n'nnGTC
Cuts at: 0 617 1662
Size: 617 1045
ApaLI G'TGCA_C
Cuts at: 0 129 914 1662
Size: 129 785 748
Fragments arranged by size:
785 748 129
ApoI r'AATT_y
Cuts at: 0 138 344 565 1662
Size: 138 206 221 1097
Fragments arranged by size:
1097 221 206 138
BanI G'GyrC_C
Cuts at: 0 18 1662
Size: 18 1644
BbsI GAAGACnn'nnnn_
Cuts at: 0 644 1066 1662
Size: 644 422 596
Fragments arranged by size:
644 596 422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 994 1028 1662
Size: 994 34 634
Fragments arranged by size:
994 634 34
BfrBI ATG'CAT
Cuts at: 0 553 1662
Size: 553 1109
Bme1580I G_kGCm'C
Cuts at: 0 133 918 1662
Size: 133 785 744
Fragments arranged by size:
785 744 133
BmrI ACTGGGnnnn_n'
Cuts at: 0 1496 1662
Size: 1496 166
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 1662
Size: 1079 583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 1662
Size: 806 856
BsaAI yAC'GTr
Cuts at: 0 1431 1662
Size: 1431 231
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 1494 1524 1662
Size: 112 30 1352 30 138
Fragments arranged by size:
1352 138 112 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 1496 1499 1662
Size: 133 1363 3 163
Fragments arranged by size:
1363 163 133 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1120 1662
Size: 1120 542
BsiHKAI G_wGCw'C
Cuts at: 0 133 918 1350 1662
Size: 133 785 432 312
Fragments arranged by size:
785 432 312 133
Bsp1286I G_dGCh'C
Cuts at: 0 133 918 1350 1662
Size: 133 785 432 312
Fragments arranged by size:
785 432 312 133
BstXI CCAn_nnnn'nTGG
Cuts at: 0 525 1662
Size: 525 1137
BstYI r'GATC_y
Cuts at: 0 1411 1534 1662
Size: 1411 123 128
Fragments arranged by size:
1411 128 123
Bsu36I CC'TnA_GG
Cuts at: 0 1076 1662
Size: 1076 586
BtgI C'CryG_G
Cuts at: 0 539 953 1662
Size: 539 414 709
Fragments arranged by size:
709 539 414
DrdI GACnn_nn'nnGTC
Cuts at: 0 235 1662
Size: 235 1427
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 27 1662
Size: 27 1635
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 384 1079 1139 1388 1662
Size: 279 105 695 60 249 274
Fragments arranged by size:
695 279 274 249 105 60
EcoO109I rG'GnC_Cy
Cuts at: 0 205 619 1662
Size: 205 414 1043
Fragments arranged by size:
1043 414 205
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1253 1662
Size: 1253 409
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 603 635 804 836 1662
Size: 603 32 169 32 826
Fragments arranged by size:
826 603 169 32 32
HincII GTy'rAC
Cuts at: 0 1020 1662
Size: 1020 642
HindIII A'AGCT_T
Cuts at: 0 448 1662
Size: 448 1214
KpnI G_GTAC'C
Cuts at: 0 22 1662
Size: 22 1640
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1389 1662
Size: 1389 273
MspA1I CmG'CkG
Cuts at: 0 1090 1324 1662
Size: 1090 234 338
Fragments arranged by size:
1090 338 234
NcoI C'CATG_G
Cuts at: 0 539 953 1662
Size: 539 414 709
Fragments arranged by size:
709 539 414
NsiI A_TGCA'T
Cuts at: 0 555 1662
Size: 555 1107
NspI r_CATG'y
Cuts at: 0 557 1662
Size: 557 1105
PflMI CCAn_nnn'nTGG
Cuts at: 0 959 1531 1662
Size: 959 572 131
Fragments arranged by size:
959 572 131
PfoI T'CCnGG_A
Cuts at: 0 152 192 1662
Size: 152 40 1470
Fragments arranged by size:
1470 152 40
PmlI CAC'GTG
Cuts at: 0 1431 1662
Size: 1431 231
PpuMI rG'GwC_Cy
Cuts at: 0 205 619 1662
Size: 205 414 1043
Fragments arranged by size:
1043 414 205
PstI C_TGCA'G
Cuts at: 0 1133 1341 1451 1662
Size: 1133 208 110 211
Fragments arranged by size:
1133 211 208 110
PvuII CAG'CTG
Cuts at: 0 1324 1662
Size: 1324 338
SfcI C'TryA_G
Cuts at: 0 1129 1337 1447 1662
Size: 1129 208 110 215
Fragments arranged by size:
1129 215 208 110
SmlI C'TyrA_G
Cuts at: 0 821 1662
Size: 821 841
SphI G_CATG'C
Cuts at: 0 557 1662
Size: 557 1105
SspI AAT'ATT
Cuts at: 0 1035 1662
Size: 1035 627
StyI C'CwwG_G
Cuts at: 0 539 953 1662
Size: 539 414 709
Fragments arranged by size:
709 539 414
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1265 1662
Size: 1265 397
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 112 144 1662
Size: 112 32 1518
Fragments arranged by size:
1518 112 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 1038 1662
Size: 525 513 624
Fragments arranged by size:
624 525 513
XmnI GAAnn'nnTTC
Cuts at: 0 851 1224 1401 1662
Size: 851 373 177 261
Fragments arranged by size:
851 373 261 177
Enzymes that do cut:
Acc65I AcuI AflIII AhdI ApaLI ApoI BanI BbsI
BcgI BfrBI Bme1580I BmrI BpmI BpuEI BsaAI BsaXI
BseRI BsgI BsiHKAI Bsp1286I BstXI BstYI Bsu36I BtgI
DrdI EciI Eco57MI EcoO109I FalI Hin4I HincII HindIII
KpnI MmeI MspA1I NcoI NsiI NspI PflMI PfoI
PmlI PpuMI PstI PvuII SfcI SmlI SphI SspI
StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BciVI BclI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI
BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII EaeI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI TatI Tth111I XbaI XhoI XmaI ZraI