Restriction map for
pFN21AE5056
(Linear) (Six-base) MAPSORT of: KIEE5056.seq Check: 9393 from: 1 to: 672
KIEE5056.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AccI GT'mk_AC
Cuts at: 0 456 672
Size: 456 216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 526 672
Size: 517 9 146
Fragments arranged by size:
517 146 9
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 302 672
Size: 302 370
AlwNI CAG_nnn'CTG
Cuts at: 0 380 672
Size: 380 292
ApoI r'AATT_y
Cuts at: 0 157 567 672
Size: 157 410 105
Fragments arranged by size:
410 157 105
AvaI C'yCGr_G
Cuts at: 0 257 672
Size: 257 415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 238 272 672
Size: 238 34 400
Fragments arranged by size:
400 238 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 256 672
Size: 256 416
BglI GCCn_nnn'nGGC
Cuts at: 0 34 672
Size: 34 638
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 57 672
Size: 57 615
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 357 672
Size: 82 275 315
Fragments arranged by size:
315 275 82
Bpu10I CC'TnA_GC
Cuts at: 0 415 672
Size: 415 257
BsaWI w'CCGG_w
Cuts at: 0 167 672
Size: 167 505
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 487 661 672
Size: 487 174 11
Fragments arranged by size:
487 174 11
BseYI C'CCAG_C
Cuts at: 0 336 672
Size: 336 336
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 404 672
Size: 268 136 268
Fragments arranged by size:
268 268 136
BspEI T'CCGG_A
Cuts at: 0 167 672
Size: 167 505
BspHI T'CATG_A
Cuts at: 0 533 672
Size: 533 139
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 440 672
Size: 440 232
BstAPI GCAn_nnn'nTGC
Cuts at: 0 391 672
Size: 391 281
BstBI TT'CG_AA
Cuts at: 0 551 672
Size: 551 121
BstYI r'GATC_y
Cuts at: 0 57 482 672
Size: 57 425 190
Fragments arranged by size:
425 190 57
DraI TTT'AAA
Cuts at: 0 156 672
Size: 156 516
EaeI y'GGCC_r
Cuts at: 0 26 672
Size: 26 646
EarI CTCTTCn'nnn_
Cuts at: 0 8 466 578 672
Size: 8 458 112 94
Fragments arranged by size:
458 112 94 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 357 517 526 672
Size: 82 275 160 9 146
Fragments arranged by size:
275 160 146 82 9
FspI TGC'GCA
Cuts at: 0 529 672
Size: 529 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 672
Size: 577 32 63
Fragments arranged by size:
577 63 32
HincII GTy'rAC
Cuts at: 0 457 672
Size: 457 215
HindIII A'AGCT_T
Cuts at: 0 302 672
Size: 302 370
MscI TGG'CCA
Cuts at: 0 28 672
Size: 28 644
MslI CAynn'nnrTG
Cuts at: 0 448 672
Size: 448 224
NspI r_CATG'y
Cuts at: 0 283 672
Size: 283 389
PasI CC'CwG_GG
Cuts at: 0 611 672
Size: 611 61
PfoI T'CCnGG_A
Cuts at: 0 187 672
Size: 187 485
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 622 654 672
Size: 622 32 18
Fragments arranged by size:
622 32 18
SalI G'TCGA_C
Cuts at: 0 455 672
Size: 455 217
SexAI A'CCwGG_T
Cuts at: 0 358 672
Size: 358 314
SphI G_CATG'C
Cuts at: 0 283 672
Size: 283 389
StuI AGG'CCT
Cuts at: 0 601 672
Size: 601 71
StyI C'CwwG_G
Cuts at: 0 596 672
Size: 596 76
TatI w'GTAC_w
Cuts at: 0 179 672
Size: 179 493
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApoI AvaI BcgI BciVI
BglI BplI BpmI Bpu10I BsaWI BseRI BseYI BsgI
BspEI BspHI BspMI BstAPI BstBI BstYI DraI EaeI
EarI Eco57MI FspI Hin4I HincII HindIII MscI MslI
NspI PasI PfoI PsrI SalI SexAI SphI StuI
StyI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HaeII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI