Restriction map for
pFN21AE5055
(Linear) (Six-base) MAPSORT of: KIEE5055.seq Check: 6027 from: 1 to: 591
KIEE5055.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AccI GT'mk_AC
Cuts at: 0 419 591
Size: 419 172
AfeI AGC'GCT
Cuts at: 0 7 591
Size: 7 584
AflIII A'CryG_T
Cuts at: 0 356 591
Size: 356 235
AhdI GACnn_n'nnGTC
Cuts at: 0 519 591
Size: 519 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 414 591
Size: 64 350 177
Fragments arranged by size:
350 177 64
ApaI G_GGCC'C
Cuts at: 0 192 266 591
Size: 192 74 325
Fragments arranged by size:
325 192 74
ApaLI G'TGCA_C
Cuts at: 0 173 556 591
Size: 173 383 35
Fragments arranged by size:
383 173 35
AvaI C'yCGr_G
Cuts at: 0 427 591
Size: 427 164
BanI G'GyrC_C
Cuts at: 0 394 455 591
Size: 394 61 136
Fragments arranged by size:
394 136 61
BanII G_rGCy'C
Cuts at: 0 192 266 591
Size: 192 74 325
Fragments arranged by size:
325 192 74
BbeI G_GCGC'C
Cuts at: 0 398 459 591
Size: 398 61 132
Fragments arranged by size:
398 132 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 189 223 591
Size: 189 34 368
Fragments arranged by size:
368 189 34
BglI GCCn_nnn'nGGC
Cuts at: 0 160 270 309 403 591
Size: 160 110 39 94 188
Fragments arranged by size:
188 160 110 94 39
Bme1580I G_kGCm'C
Cuts at: 0 177 192 266 560 591
Size: 177 15 74 294 31
Fragments arranged by size:
294 177 74 31 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 159 591
Size: 159 432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 591
Size: 343 248
BsaHI Gr'CG_yC
Cuts at: 0 143 220 395 456 591
Size: 143 77 175 61 135
Fragments arranged by size:
175 143 135 77 61
BseYI C'CCAG_C
Cuts at: 0 229 342 591
Size: 229 113 249
Fragments arranged by size:
249 229 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 591
Size: 103 488
BsiHKAI G_wGCw'C
Cuts at: 0 38 177 560 591
Size: 38 139 383 31
Fragments arranged by size:
383 139 38 31
BsmBI CGTCTCn'nnnn_
Cuts at: 0 361 591
Size: 361 230
Bsp1286I G_dGCh'C
Cuts at: 0 38 177 192 266 560 591
Size: 38 139 15 74 294 31
Fragments arranged by size:
294 139 74 38 31 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 111 591
Size: 111 480
BsrBI CCG'CTC
Cuts at: 0 471 591
Size: 471 120
BssHII G'CGCG_C
Cuts at: 0 276 278 412 477 479 571 591
Size: 276 2 134 65 2 92 20
Fragments arranged by size:
276 134 92 65 20 2 2
BssSI C'ACGA_G
Cuts at: 0 559 591
Size: 559 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 126 591
Size: 126 465
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 214 591
Size: 214 377
DraIII CAC_nnn'GTG
Cuts at: 0 555 591
Size: 555 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 591
Size: 343 248
EcoO109I rG'GnC_Cy
Cuts at: 0 75 188 189 262 263 591
Size: 75 113 1 73 1 328
Fragments arranged by size:
328 113 75 73 1 1
FspI TGC'GCA
Cuts at: 0 390 591
Size: 390 201
HaeII r_GCGC'y
Cuts at: 0 9 398 459 591
Size: 9 389 61 132
Fragments arranged by size:
389 132 61 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 591
Size: 505 32 54
Fragments arranged by size:
505 54 32
KasI G'GCGC_C
Cuts at: 0 394 455 591
Size: 394 61 136
Fragments arranged by size:
394 136 61
MluI A'CGCG_T
Cuts at: 0 356 591
Size: 356 235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 591
Size: 386 205
MslI CAynn'nnrTG
Cuts at: 0 61 591
Size: 61 530
MspA1I CmG'CkG
Cuts at: 0 450 591
Size: 450 141
NarI GG'CG_CC
Cuts at: 0 395 456 591
Size: 395 61 135
Fragments arranged by size:
395 135 61
PasI CC'CwG_GG
Cuts at: 0 224 591
Size: 224 367
PfoI T'CCnGG_A
Cuts at: 0 527 591
Size: 527 64
PspOMI G'GGCC_C
Cuts at: 0 188 262 591
Size: 188 74 329
Fragments arranged by size:
329 188 74
PstI C_TGCA'G
Cuts at: 0 43 185 591
Size: 43 142 406
Fragments arranged by size:
406 142 43
SbfI CC_TGCA'GG
Cuts at: 0 185 591
Size: 185 406
SfcI C'TryA_G
Cuts at: 0 39 181 591
Size: 39 142 410
Fragments arranged by size:
410 142 39
SfoI GGC'GCC
Cuts at: 0 396 457 591
Size: 396 61 134
Fragments arranged by size:
396 134 61
StyI C'CwwG_G
Cuts at: 0 23 591
Size: 23 568
Enzymes that do cut:
AccI AfeI AflIII AhdI AlfI ApaI ApaLI AvaI
BanI BanII BbeI BcgI BglI Bme1580I BmrI BpmI
BsaHI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BssHII BssSI BstAPI BtgZI DraIII Eco57MI EcoO109I FspI
HaeII Hin4I KasI MluI MmeI MslI MspA1I NarI
PasI PfoI PspOMI PstI SbfI SfcI SfoI StyI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AgeI AleI
AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsiEI BsiWI BsmI BspEI BspHI
BsrDI BsrFI BsrGI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KpnI MfeI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI