Restriction map for
pFN21AE5053
(Linear) (Six-base) MAPSORT of: KIEE5053.seq Check: 9493 from: 1 to: 507
KIEE5053.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 150 507
Size: 150 357
ApaI G_GGCC'C
Cuts at: 0 386 507
Size: 386 121
ApaLI G'TGCA_C
Cuts at: 0 55 109 259 507
Size: 55 54 150 248
Fragments arranged by size:
248 150 55 54
AvaI C'yCGr_G
Cuts at: 0 296 507
Size: 296 211
BanI G'GyrC_C
Cuts at: 0 6 507
Size: 6 501
BanII G_rGCy'C
Cuts at: 0 278 386 507
Size: 278 108 121
Fragments arranged by size:
278 121 108
BbeI G_GCGC'C
Cuts at: 0 10 507
Size: 10 497
BbvCI CC'TCA_GC
Cuts at: 0 365 507
Size: 365 142
BlpI GC'TnA_GC
Cuts at: 0 302 371 507
Size: 302 69 136
Fragments arranged by size:
302 136 69
Bme1580I G_kGCm'C
Cuts at: 0 59 113 263 386 507
Size: 59 54 150 123 121
Fragments arranged by size:
150 123 121 59 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 181 461 507
Size: 181 280 46
Fragments arranged by size:
280 181 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 190 463 507
Size: 97 93 273 44
Fragments arranged by size:
273 97 93 44
Bpu10I CC'TnA_GC
Cuts at: 0 365 507
Size: 365 142
BsaHI Gr'CG_yC
Cuts at: 0 7 507
Size: 7 500
BseYI C'CCAG_C
Cuts at: 0 343 507
Size: 343 164
BsiHKAI G_wGCw'C
Cuts at: 0 59 113 263 278 507
Size: 59 54 150 15 229
Fragments arranged by size:
229 150 59 54 15
Bsp1286I G_dGCh'C
Cuts at: 0 59 113 263 278 386 507
Size: 59 54 150 15 108 121
Fragments arranged by size:
150 121 108 59 54 15
BsrFI r'CCGG_y
Cuts at: 0 12 59 329 390 507
Size: 12 47 270 61 117
Fragments arranged by size:
270 117 61 47 12
BssHII G'CGCG_C
Cuts at: 0 63 153 507
Size: 63 90 354
Fragments arranged by size:
354 90 63
BstYI r'GATC_y
Cuts at: 0 23 507
Size: 23 484
BtsI GCAGTG_nn'
Cuts at: 0 494 507
Size: 494 13
EaeI y'GGCC_r
Cuts at: 0 331 392 405 507
Size: 331 61 13 102
Fragments arranged by size:
331 102 61 13
EarI CTCTTCn'nnn_
Cuts at: 0 452 507
Size: 452 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 112 478 507
Size: 112 366 29
Fragments arranged by size:
366 112 29
EcoICRI GAG'CTC
Cuts at: 0 276 507
Size: 276 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 190 463 507
Size: 97 93 273 44
Fragments arranged by size:
273 97 93 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 364 507
Size: 364 143
EcoO109I rG'GnC_Cy
Cuts at: 0 382 383 507
Size: 382 1 124
Fragments arranged by size:
382 124 1
HaeII r_GCGC'y
Cuts at: 0 10 129 378 507
Size: 10 119 249 129
Fragments arranged by size:
249 129 119 10
KasI G'GCGC_C
Cuts at: 0 6 507
Size: 6 501
MspA1I CmG'CkG
Cuts at: 0 70 150 216 507
Size: 70 80 66 291
Fragments arranged by size:
291 80 70 66
NaeI GCC'GGC
Cuts at: 0 14 331 392 507
Size: 14 317 61 115
Fragments arranged by size:
317 115 61 14
NarI GG'CG_CC
Cuts at: 0 7 507
Size: 7 500
NgoMIV G'CCGG_C
Cuts at: 0 12 329 390 507
Size: 12 317 61 117
Fragments arranged by size:
317 117 61 12
PfoI T'CCnGG_A
Cuts at: 0 26 206 507
Size: 26 180 301
Fragments arranged by size:
301 180 26
PspOMI G'GGCC_C
Cuts at: 0 382 507
Size: 382 125
PstI C_TGCA'G
Cuts at: 0 23 251 314 489 507
Size: 23 228 63 175 18
Fragments arranged by size:
228 175 63 23 18
PvuII CAG'CTG
Cuts at: 0 150 216 507
Size: 150 66 291
Fragments arranged by size:
291 150 66
SacI G_AGCT'C
Cuts at: 0 278 507
Size: 278 229
SbfI CC_TGCA'GG
Cuts at: 0 251 507
Size: 251 256
SfcI C'TryA_G
Cuts at: 0 19 247 310 485 507
Size: 19 228 63 175 22
Fragments arranged by size:
228 175 63 22 19
SfoI GGC'GCC
Cuts at: 0 8 507
Size: 8 499
SgrAI Cr'CCGG_yG
Cuts at: 0 59 507
Size: 59 448
SmaI CCC'GGG
Cuts at: 0 298 507
Size: 298 209
StyI C'CwwG_G
Cuts at: 0 182 507
Size: 182 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 425 507
Size: 425 82
XmaI C'CCGG_G
Cuts at: 0 296 507
Size: 296 211
Enzymes that do cut:
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbvCI
BlpI Bme1580I BmrI BpmI Bpu10I BsaHI BseYI BsiHKAI
Bsp1286I BsrFI BssHII BstYI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI MspA1I NaeI
NarI NgoMIV PfoI PspOMI PstI PvuII SacI SbfI
SfcI SfoI SgrAI SmaI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmnI ZraI