Restriction map for
pFN21AE5024
(Linear) (Six-base) MAPSORT of: KIEE5024.seq Check: 9441 from: 1 to: 3681
KIEE5024.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AatII G_ACGT'C
Cuts at: 0 1774 3681
Size: 1774 1907
AccI GT'mk_AC
Cuts at: 0 2473 3681
Size: 2473 1208
Acc65I G'GTAC_C
Cuts at: 0 211 3681
Size: 211 3470
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1352 1765 2383 3681
Size: 1352 413 618 1298
Fragments arranged by size:
1352 1298 618 413
AfeI AGC'GCT
Cuts at: 0 3428 3681
Size: 3428 253
AflII C'TTAA_G
Cuts at: 0 3238 3681
Size: 3238 443
AflIII A'CryG_T
Cuts at: 0 2849 3121 3260 3681
Size: 2849 272 139 421
Fragments arranged by size:
2849 421 272 139
AhdI GACnn_n'nnGTC
Cuts at: 0 2630 3681
Size: 2630 1051
AleI CACnn'nnGTG
Cuts at: 0 319 1967 2599 3681
Size: 319 1648 632 1082
Fragments arranged by size:
1648 1082 632 319
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1150 1182 3681
Size: 1150 32 2499
Fragments arranged by size:
2499 1150 32
AlwNI CAG_nnn'CTG
Cuts at: 0 585 598 1067 1788 2493 3407 3681
Size: 585 13 469 721 705 914 274
Fragments arranged by size:
914 721 705 585 469 274 13
ApaI G_GGCC'C
Cuts at: 0 1501 3681
Size: 1501 2180
ApoI r'AATT_y
Cuts at: 0 2157 2698 3681
Size: 2157 541 983
Fragments arranged by size:
2157 983 541
AvaI C'yCGr_G
Cuts at: 0 244 2281 3681
Size: 244 2037 1400
Fragments arranged by size:
2037 1400 244
AvrII C'CTAG_G
Cuts at: 0 1521 3681
Size: 1521 2160
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 129 162 3681
Size: 129 33 3519
Fragments arranged by size:
3519 129 33
BamHI G'GATC_C
Cuts at: 0 2669 3393 3681
Size: 2669 724 288
Fragments arranged by size:
2669 724 288
BanI G'GyrC_C
Cuts at: 0 211 3681
Size: 211 3470
BanII G_rGCy'C
Cuts at: 0 1501 1532 1639 2897 3578 3681
Size: 1501 31 107 1258 681 103
Fragments arranged by size:
1501 1258 681 107 103 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 2204 3041 3681
Size: 2204 837 640
Fragments arranged by size:
2204 837 640
BbvCI CC'TCA_GC
Cuts at: 0 216 887 3681
Size: 216 671 2794
Fragments arranged by size:
2794 671 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3135 3169 3651 3681
Size: 3135 34 482 30
Fragments arranged by size:
3135 482 34 30
BclI T'GATC_A
Cuts at: 0 2324 3681
Size: 2324 1357
BfrBI ATG'CAT
Cuts at: 0 2070 3681
Size: 2070 1611
BglI GCCn_nnn'nGGC
Cuts at: 0 1472 1664 3681
Size: 1472 192 2017
Fragments arranged by size:
2017 1472 192
BglII A'GATC_T
Cuts at: 0 1919 2871 3461 3681
Size: 1919 952 590 220
Fragments arranged by size:
1919 952 590 220
Bme1580I G_kGCm'C
Cuts at: 0 1501 3153 3256 3681
Size: 1501 1652 103 425
Fragments arranged by size:
1652 1501 425 103
BmgBI CAC'GTC
Cuts at: 0 3030 3681
Size: 3030 651
BmrI ACTGGGnnnn_n'
Cuts at: 0 271 327 1904 2092 2593 2891 3061 3681
Size: 271 56 1577 188 501 298 170 620
Fragments arranged by size:
1577 620 501 298 271 188 170 56
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2493 3681
Size: 2493 1188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1292 3200 3547 3574 3619 3681
Size: 292 1000 1908 347 27 45 62
Fragments arranged by size:
1908 1000 347 292 62 45 27
Bpu10I CC'TnA_GC
Cuts at: 0 216 887 1250 1633 1757 3681
Size: 216 671 363 383 124 1924
Fragments arranged by size:
1924 671 383 363 216 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 3681
Size: 506 3175
BsaI GGTCTCn'nnnn_
Cuts at: 0 822 2681 2781 3681
Size: 822 1859 100 900
Fragments arranged by size:
1859 900 822 100
BsaHI Gr'CG_yC
Cuts at: 0 1771 2243 3681
Size: 1771 472 1438
Fragments arranged by size:
1771 1438 472
BsaWI w'CCGG_w
Cuts at: 0 1123 3681
Size: 1123 2558
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1077 1107 1150 1180 2365 2395 3448 3478
Size: 1077 30 43 30 1185 30 1053 30
Cuts at: 3478 3681
Size: 203
Fragments arranged by size:
1185 1077 1053 203 43 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 192 2557 2987 3129 3681
Size: 192 2365 430 142 552
Fragments arranged by size:
2365 552 430 192 142
BseYI C'CCAG_C
Cuts at: 0 387 787 810 916 977 1641 2457 2533
Size: 387 400 23 106 61 664 816 76
Cuts at: 2533 2882 3681
Size: 349 799
Fragments arranged by size:
816 799 664 400 387 349 106 76
61 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1080 3263 3681
Size: 1080 2183 418
Fragments arranged by size:
2183 1080 418
BsiHKAI G_wGCw'C
Cuts at: 0 316 1639 3681
Size: 316 1323 2042
Fragments arranged by size:
2042 1323 316
BsmI GAATG_Cn'
Cuts at: 0 495 1042 2004 2072 3681
Size: 495 547 962 68 1609
Fragments arranged by size:
1609 962 547 495 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1762 3681
Size: 1762 1919
Bsp1286I G_dGCh'C
Cuts at: 0 316 1501 1532 1639 2897 3153 3256 3578
Size: 316 1185 31 107 1258 256 103 322
Cuts at: 3578 3681
Size: 103
Fragments arranged by size:
1258 1185 322 316 256 107 103 103
31
BspHI T'CATG_A
Cuts at: 0 3162 3681
Size: 3162 519
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1052 3681
Size: 1052 2629
BsrBI CCG'CTC
Cuts at: 0 1701 1992 2560 3681
Size: 1701 291 568 1121
Fragments arranged by size:
1701 1121 568 291
BsrFI r'CCGG_y
Cuts at: 0 3592 3681
Size: 3592 89
BssSI C'ACGA_G
Cuts at: 0 1272 3675 3681
Size: 1272 2403 6
Fragments arranged by size:
2403 1272 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1067 1151 1838 2807 3681
Size: 1067 84 687 969 874
Fragments arranged by size:
1067 969 874 687 84
BstEII G'GTnAC_C
Cuts at: 0 1596 3681
Size: 1596 2085
BstXI CCAn_nnnn'nTGG
Cuts at: 0 87 2449 3441 3681
Size: 87 2362 992 240
Fragments arranged by size:
2362 992 240 87
BstYI r'GATC_y
Cuts at: 0 428 1103 1919 2084 2669 2871 3393 3461
Size: 428 675 816 165 585 202 522 68
Cuts at: 3461 3681
Size: 220
Fragments arranged by size:
816 675 585 522 428 220 202 165
68
Bsu36I CC'TnA_GG
Cuts at: 0 2207 2999 3681
Size: 2207 792 682
Fragments arranged by size:
2207 792 682
BtgI C'CryG_G
Cuts at: 0 80 1661 2123 3681
Size: 80 1581 462 1558
Fragments arranged by size:
1581 1558 462 80
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2280 3460 3681
Size: 2280 1180 221
Fragments arranged by size:
2280 1180 221
BtsI GCAGTG_nn'
Cuts at: 0 1007 1674 2708 3519 3621 3681
Size: 1007 667 1034 811 102 60
Fragments arranged by size:
1034 1007 811 667 102 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3105 3140 3681
Size: 3105 35 541
Fragments arranged by size:
3105 541 35
DraI TTT'AAA
Cuts at: 0 956 2703 3681
Size: 956 1747 978
Fragments arranged by size:
1747 978 956
EaeI y'GGCC_r
Cuts at: 0 380 1926 3590 3681
Size: 380 1546 1664 91
Fragments arranged by size:
1664 1546 380 91
EarI CTCTTCn'nnn_
Cuts at: 0 68 723 1421 1577 2271 2484 2680 3207
Size: 68 655 698 156 694 213 196 527
Cuts at: 3207 3681
Size: 474
Fragments arranged by size:
698 694 655 527 474 213 196 156
68
EcoICRI GAG'CTC
Cuts at: 0 1637 3681
Size: 1637 2044
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1292 1352 1765 2383 3200 3547 3574
Size: 292 1000 60 413 618 817 347 27
Cuts at: 3574 3619 3681
Size: 45 62
Fragments arranged by size:
1000 817 618 413 347 292 62 60
45 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 521 3681
Size: 521 3160
EcoO109I rG'GnC_Cy
Cuts at: 0 93 249 362 791 1497 1498 1904 3681
Size: 93 156 113 429 706 1 406 1777
Fragments arranged by size:
1777 706 429 406 156 113 93 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1969 3253 3681
Size: 1969 1284 428
Fragments arranged by size:
1969 1284 428
FseI GG_CCGG'CC
Cuts at: 0 3596 3681
Size: 3596 85
HaeII r_GCGC'y
Cuts at: 0 1203 1868 3430 3681
Size: 1203 665 1562 251
Fragments arranged by size:
1562 1203 665 251
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 943 975 2069 2098 2101 2130 2164 2196
Size: 943 32 1094 29 3 29 34 32
Cuts at: 2196 2245 2277 2365 2397 2401 2433 2611 2616
Size: 49 32 88 32 4 32 178 5
Cuts at: 2616 2643 2648 2856 2863 2888 2895 3681
Size: 27 5 208 7 25 7 786
Fragments arranged by size:
1094 943 786 208 178 88 49 34
32 32 32 32 32 29 29 27
25 7 7 5 5 4 3
HincII GTy'rAC
Cuts at: 0 15 2855 2976 3681
Size: 15 2840 121 705
Fragments arranged by size:
2840 705 121 15
HindIII A'AGCT_T
Cuts at: 0 1777 3681
Size: 1777 1904
HpaI GTT'AAC
Cuts at: 0 15 3681
Size: 15 3666
KpnI G_GTAC'C
Cuts at: 0 215 3681
Size: 215 3466
MfeI C'AATT_G
Cuts at: 0 442 3681
Size: 442 3239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 2528 2927 3458 3681
Size: 731 1797 399 531 223
Fragments arranged by size:
1797 731 531 399 223
MslI CAynn'nnrTG
Cuts at: 0 85 319 1140 1967 2065 2599 3259 3681
Size: 85 234 821 827 98 534 660 422
Fragments arranged by size:
827 821 660 534 422 234 98 85
MspA1I CmG'CkG
Cuts at: 0 119 131 185 438 585 598 713 745
Size: 119 12 54 253 147 13 115 32
Cuts at: 745 814 1328 1645 1663 1856 2929 3681
Size: 69 514 317 18 193 1073 752
Fragments arranged by size:
1073 752 514 317 253 193 147 119
115 69 54 32 18 13 12
NaeI GCC'GGC
Cuts at: 0 3594 3681
Size: 3594 87
NcoI C'CATG_G
Cuts at: 0 80 2123 3681
Size: 80 2043 1558
Fragments arranged by size:
2043 1558 80
NdeI CA'TA_TG
Cuts at: 0 1290 2072 3681
Size: 1290 782 1609
Fragments arranged by size:
1609 1290 782
NgoMIV G'CCGG_C
Cuts at: 0 3592 3681
Size: 3592 89
NsiI A_TGCA'T
Cuts at: 0 2072 3681
Size: 2072 1609
NspI r_CATG'y
Cuts at: 0 1299 2853 2992 3125 3264 3681
Size: 1299 1554 139 133 139 417
Fragments arranged by size:
1554 1299 417 139 139 133
PciI A'CATG_T
Cuts at: 0 2849 3121 3260 3681
Size: 2849 272 139 421
Fragments arranged by size:
2849 421 272 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 1567 2605 3407 3499 3681
Size: 1567 1038 802 92 182
Fragments arranged by size:
1567 1038 802 182 92
PfoI T'CCnGG_A
Cuts at: 0 1745 3498 3681
Size: 1745 1753 183
Fragments arranged by size:
1753 1745 183
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1150 1182 3681
Size: 1150 32 2499
Fragments arranged by size:
2499 1150 32
PpuMI rG'GwC_Cy
Cuts at: 0 249 3681
Size: 249 3432
PshAI GACnn'nnGTC
Cuts at: 0 2971 3681
Size: 2971 710
PspOMI G'GGCC_C
Cuts at: 0 1497 3681
Size: 1497 2184
PstI C_TGCA'G
Cuts at: 0 40 419 948 2054 2241 3158 3681
Size: 40 379 529 1106 187 917 523
Fragments arranged by size:
1106 917 529 523 379 187 40
PvuII CAG'CTG
Cuts at: 0 438 585 598 1328 1856 3681
Size: 438 147 13 730 528 1825
Fragments arranged by size:
1825 730 528 438 147 13
SacI G_AGCT'C
Cuts at: 0 1639 3681
Size: 1639 2042
SacII CC_GC'GG
Cuts at: 0 1664 3681
Size: 1664 2017
SapI GCTCTTCn'nnn_
Cuts at: 0 723 1421 3207 3681
Size: 723 698 1786 474
Fragments arranged by size:
1786 723 698 474
ScaI AGT'ACT
Cuts at: 0 1029 1278 3681
Size: 1029 249 2403
Fragments arranged by size:
2403 1029 249
SfcI C'TryA_G
Cuts at: 0 36 415 944 1881 1965 2050 2237 2350
Size: 36 379 529 937 84 85 187 113
Cuts at: 2350 2766 3154 3681
Size: 416 388 527
Fragments arranged by size:
937 529 527 416 388 379 187 113
85 84 36
SmlI C'TyrA_G
Cuts at: 0 521 2281 3238 3681
Size: 521 1760 957 443
Fragments arranged by size:
1760 957 521 443
SspI AAT'ATT
Cuts at: 0 470 3681
Size: 470 3211
StyI C'CwwG_G
Cuts at: 0 80 317 971 1172 1521 2123 2753 2907
Size: 80 237 654 201 349 602 630 154
Cuts at: 2907 3681
Size: 774
Fragments arranged by size:
774 654 630 602 349 237 201 154
80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2413 2547 3681
Size: 2413 134 1134
Fragments arranged by size:
2413 1134 134
TatI w'GTAC_w
Cuts at: 0 1027 1276 3681
Size: 1027 249 2405
Fragments arranged by size:
2405 1027 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 369 684 716 1077 1099 1109 1131
Size: 337 32 315 32 361 22 10 22
Cuts at: 1131 3681
Size: 2550
Fragments arranged by size:
2550 361 337 315 32 32 22 22
10
Tth111I GACn'n_nGTC
Cuts at: 0 3287 3681
Size: 3287 394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 1325 3585 3681
Size: 324 1001 2260 96
Fragments arranged by size:
2260 1001 324 96
XhoI C'TCGA_G
Cuts at: 0 2281 3681
Size: 2281 1400
XmnI GAAnn'nnTTC
Cuts at: 0 455 1162 2029 3681
Size: 455 707 867 1652
Fragments arranged by size:
1652 867 707 455
ZraI GAC'GTC
Cuts at: 0 1772 3681
Size: 1772 1909
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflII AflIII AhdI
AleI AloI AlwNI ApaI ApoI AvaI AvrII BaeI
BamHI BanI BanII BbsI BbvCI BcgI BclI BfrBI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MmeI MslI MspA1I NaeI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SapI ScaI
SfcI SmlI SspI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AclI AgeI AlfI ApaLI AscI AseI AsiSI
BbeI BciVI BlpI BmtI BsaAI BsaBI BsiEI BsiWI
BspEI BsrDI BsrGI BssHII BstBI BstZ17I ClaI DraIII
DrdI EagI EciI EcoRI EcoRV FspI FspAI KasI
MluI MscI NarI NheI NotI NruI PacI PasI
PmeI PmlI PsiI PspXI PsrI PvuI RsrII SalI
SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI XbaI XmaI