Restriction map for
pFN21AE5023
(Linear) (Six-base) MAPSORT of: KIEE5023.seq Check: 5525 from: 1 to: 1401
KIEE5023.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1140 1401
Size: 1140 261
AfeI AGC'GCT
Cuts at: 0 352 1401
Size: 352 1049
AflIII A'CryG_T
Cuts at: 0 791 1401
Size: 791 610
AleI CACnn'nnGTG
Cuts at: 0 574 1401
Size: 574 827
ApaI G_GGCC'C
Cuts at: 0 289 1342 1401
Size: 289 1053 59
Fragments arranged by size:
1053 289 59
ApaLI G'TGCA_C
Cuts at: 0 1021 1401
Size: 1021 380
AscI GG'CGCG_CC
Cuts at: 0 340 907 1401
Size: 340 567 494
Fragments arranged by size:
567 494 340
AvaI C'yCGr_G
Cuts at: 0 21 458 516 822 1144 1276 1401
Size: 21 437 58 306 322 132 125
Fragments arranged by size:
437 322 306 132 125 58 21
BanI G'GyrC_C
Cuts at: 0 485 1401
Size: 485 916
BanII G_rGCy'C
Cuts at: 0 289 439 1247 1342 1401
Size: 289 150 808 95 59
Fragments arranged by size:
808 289 150 95 59
BbeI G_GCGC'C
Cuts at: 0 489 1401
Size: 489 912
BbvCI CC'TCA_GC
Cuts at: 0 1174 1401
Size: 1174 227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 519 553 639 673 1401
Size: 519 34 86 34 728
Fragments arranged by size:
728 519 86 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 106 247 571 1401
Size: 106 141 324 830
Fragments arranged by size:
830 324 141 106
BlpI GC'TnA_GC
Cuts at: 0 1162 1401
Size: 1162 239
Bme1580I G_kGCm'C
Cuts at: 0 289 1025 1073 1342 1401
Size: 289 736 48 269 59
Fragments arranged by size:
736 289 269 59 48
BmgBI CAC'GTC
Cuts at: 0 57 192 498 1401
Size: 57 135 306 903
Fragments arranged by size:
903 306 135 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 333 1401
Size: 333 1068
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1347 1401
Size: 1347 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 367 685 862 1047 1401
Size: 151 216 318 177 185 354
Fragments arranged by size:
354 318 216 185 177 151
Bpu10I CC'TnA_GC
Cuts at: 0 589 1174 1401
Size: 589 585 227
Fragments arranged by size:
589 585 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1231 1401
Size: 1231 170
BsaI GGTCTCn'nnnn_
Cuts at: 0 661 1401
Size: 661 740
BsaAI yAC'GTr
Cuts at: 0 792 894 1401
Size: 792 102 507
Fragments arranged by size:
792 507 102
BsaHI Gr'CG_yC
Cuts at: 0 486 1401
Size: 486 915
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 658 688 1401
Size: 658 30 713
Fragments arranged by size:
713 658 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 397 400 631 634 1401
Size: 397 3 231 3 767
Fragments arranged by size:
767 397 231 3 3
BseYI C'CCAG_C
Cuts at: 0 804 918 933 1041 1401
Size: 804 114 15 108 360
Fragments arranged by size:
804 360 114 108 15
BsiEI CG_ry'CG
Cuts at: 0 219 395 480 575 1401
Size: 219 176 85 95 826
Fragments arranged by size:
826 219 176 95 85
BsiHKAI G_wGCw'C
Cuts at: 0 595 637 890 1025 1247 1401
Size: 595 42 253 135 222 154
Fragments arranged by size:
595 253 222 154 135 42
Bsp1286I G_dGCh'C
Cuts at: 0 289 439 595 637 890 1025 1073 1247
Size: 289 150 156 42 253 135 48 174
Cuts at: 1247 1342 1401
Size: 95 59
Fragments arranged by size:
289 253 174 156 150 135 95 59
48 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 1140 1401
Size: 173 967 261
Fragments arranged by size:
967 261 173
BsrBI CCG'CTC
Cuts at: 0 624 1401
Size: 624 777
BsrFI r'CCGG_y
Cuts at: 0 479 1281 1401
Size: 479 802 120
Fragments arranged by size:
802 479 120
BssHII G'CGCG_C
Cuts at: 0 124 141 258 293 340 442 444 744
Size: 124 17 117 35 47 102 2 300
Cuts at: 744 746 846 907 956 958 1046 1048 1263
Size: 2 100 61 49 2 88 2 215
Cuts at: 1263 1401
Size: 138
Fragments arranged by size:
300 215 138 124 117 102 100 88
61 49 47 35 17 2 2 2
2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 227 1401
Size: 227 1174
BstEII G'GTnAC_C
Cuts at: 0 1203 1401
Size: 1203 198
BstXI CCAn_nnnn'nTGG
Cuts at: 0 840 1401
Size: 840 561
BtgI C'CryG_G
Cuts at: 0 7 150 575 998 1109 1370 1401
Size: 7 143 425 423 111 261 31
Fragments arranged by size:
425 423 261 143 111 31 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 126 1401
Size: 126 1275
EaeI y'GGCC_r
Cuts at: 0 153 392 477 563 572 995 1028 1268
Size: 153 239 85 86 9 423 33 240
Cuts at: 1268 1310 1358 1401
Size: 42 48 43
Fragments arranged by size:
423 240 239 153 86 85 48 43
42 33 9
EagI C'GGCC_G
Cuts at: 0 392 477 572 1401
Size: 392 85 95 829
Fragments arranged by size:
829 392 95 85
EarI CTCTTCn'nnn_
Cuts at: 0 541 1012 1401
Size: 541 471 389
Fragments arranged by size:
541 471 389
EcoICRI GAG'CTC
Cuts at: 0 1245 1401
Size: 1245 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 367 685 862 1047 1401
Size: 151 216 318 177 185 354
Fragments arranged by size:
354 318 216 185 177 151
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1291 1401
Size: 1291 110
EcoO109I rG'GnC_Cy
Cuts at: 0 41 285 454 1338 1339 1401
Size: 41 244 169 884 1 62
Fragments arranged by size:
884 244 169 62 41 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 753 1401
Size: 753 648
HaeII r_GCGC'y
Cuts at: 0 138 253 354 489 778 1006 1162 1401
Size: 138 115 101 135 289 228 156 239
Fragments arranged by size:
289 239 228 156 138 135 115 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 104 136 1401
Size: 104 32 1265
Fragments arranged by size:
1265 104 32
KasI G'GCGC_C
Cuts at: 0 485 1401
Size: 485 916
MscI TGG'CCA
Cuts at: 0 997 1401
Size: 997 404
MslI CAynn'nnrTG
Cuts at: 0 67 574 1099 1401
Size: 67 507 525 302
Fragments arranged by size:
525 507 302 67
MspA1I CmG'CkG
Cuts at: 0 152 953 1178 1372 1401
Size: 152 801 225 194 29
Fragments arranged by size:
801 225 194 152 29
NaeI GCC'GGC
Cuts at: 0 481 1283 1401
Size: 481 802 118
Fragments arranged by size:
802 481 118
NarI GG'CG_CC
Cuts at: 0 486 1401
Size: 486 915
NcoI C'CATG_G
Cuts at: 0 7 998 1401
Size: 7 991 403
Fragments arranged by size:
991 403 7
NgoMIV G'CCGG_C
Cuts at: 0 479 1281 1401
Size: 479 802 120
Fragments arranged by size:
802 479 120
PasI CC'CwG_GG
Cuts at: 0 450 1401
Size: 450 951
PflMI CCAn_nnn'nTGG
Cuts at: 0 918 1082 1401
Size: 918 164 319
Fragments arranged by size:
918 319 164
PfoI T'CCnGG_A
Cuts at: 0 839 1401
Size: 839 562
PpuMI rG'GwC_Cy
Cuts at: 0 41 454 1401
Size: 41 413 947
Fragments arranged by size:
947 413 41
PspOMI G'GGCC_C
Cuts at: 0 285 1338 1401
Size: 285 1053 63
Fragments arranged by size:
1053 285 63
PstI C_TGCA'G
Cuts at: 0 473 719 1292 1401
Size: 473 246 573 109
Fragments arranged by size:
573 473 246 109
SacI G_AGCT'C
Cuts at: 0 1247 1401
Size: 1247 154
SacII CC_GC'GG
Cuts at: 0 153 1373 1401
Size: 153 1220 28
Fragments arranged by size:
1220 153 28
SanDI GG'GwC_CC
Cuts at: 0 41 1401
Size: 41 1360
SapI GCTCTTCn'nnn_
Cuts at: 0 541 1012 1401
Size: 541 471 389
Fragments arranged by size:
541 471 389
SbfI CC_TGCA'GG
Cuts at: 0 719 1292 1401
Size: 719 573 109
Fragments arranged by size:
719 573 109
SfcI C'TryA_G
Cuts at: 0 469 715 1288 1401
Size: 469 246 573 113
Fragments arranged by size:
573 469 246 113
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 571 1401
Size: 571 830
SfoI GGC'GCC
Cuts at: 0 487 1401
Size: 487 914
SmaI CCC'GGG
Cuts at: 0 23 824 1146 1401
Size: 23 801 322 255
Fragments arranged by size:
801 322 255 23
SmlI C'TyrA_G
Cuts at: 0 1246 1401
Size: 1246 155
SrfI GCCC'GGGC
Cuts at: 0 824 1146 1401
Size: 824 322 255
Fragments arranged by size:
824 322 255
StyI C'CwwG_G
Cuts at: 0 7 998 1401
Size: 7 991 403
Fragments arranged by size:
991 403 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1374 1401
Size: 1374 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 658 690 1366 1398 1401
Size: 658 32 676 32 3
Fragments arranged by size:
676 658 32 32 3
Tth111I GACn'n_nGTC
Cuts at: 0 1126 1401
Size: 1126 275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1107 1401
Size: 1107 294
XmaI C'CCGG_G
Cuts at: 0 21 822 1144 1401
Size: 21 801 322 257
Fragments arranged by size:
801 322 257 21
Enzymes that do cut:
AarI AfeI AflIII AleI ApaI ApaLI AscI AvaI
BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BstAPI BstEII BstXI BtgI BtgZI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI
SacI SacII SanDI SapI SbfI SfcI SfiI SfoI
SmaI SmlI SrfI StyI TaqII TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AlfI AloI AlwNI ApoI AseI AsiSI AvrII BaeI
BamHI BbsI BciVI BclI BfrBI BglII BmtI BsaBI
BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BssSI BstBI BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EciI EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SalI ScaI SexAI SgfI SgrAI
SnaBI SpeI SphI SspI StuI SwaI TatI XbaI
XhoI XmnI ZraI