(Linear) (Six-base) MAPSORT of: KIEE5023.seq Check: 5525 from: 1 to: 1401 KIEE5023.orf With 182 enzymes: SgfI * December 5, 2014 12:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1140 1401 Size: 1140 261 AfeI AGC'GCT Cuts at: 0 352 1401 Size: 352 1049 AflIII A'CryG_T Cuts at: 0 791 1401 Size: 791 610 AleI CACnn'nnGTG Cuts at: 0 574 1401 Size: 574 827 ApaI G_GGCC'C Cuts at: 0 289 1342 1401 Size: 289 1053 59 Fragments arranged by size: 1053 289 59 ApaLI G'TGCA_C Cuts at: 0 1021 1401 Size: 1021 380 AscI GG'CGCG_CC Cuts at: 0 340 907 1401 Size: 340 567 494 Fragments arranged by size: 567 494 340 AvaI C'yCGr_G Cuts at: 0 21 458 516 822 1144 1276 1401 Size: 21 437 58 306 322 132 125 Fragments arranged by size: 437 322 306 132 125 58 21 BanI G'GyrC_C Cuts at: 0 485 1401 Size: 485 916 BanII G_rGCy'C Cuts at: 0 289 439 1247 1342 1401 Size: 289 150 808 95 59 Fragments arranged by size: 808 289 150 95 59 BbeI G_GCGC'C Cuts at: 0 489 1401 Size: 489 912 BbvCI CC'TCA_GC Cuts at: 0 1174 1401 Size: 1174 227 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 519 553 639 673 1401 Size: 519 34 86 34 728 Fragments arranged by size: 728 519 86 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 106 247 571 1401 Size: 106 141 324 830 Fragments arranged by size: 830 324 141 106 BlpI GC'TnA_GC Cuts at: 0 1162 1401 Size: 1162 239 Bme1580I G_kGCm'C Cuts at: 0 289 1025 1073 1342 1401 Size: 289 736 48 269 59 Fragments arranged by size: 736 289 269 59 48 BmgBI CAC'GTC Cuts at: 0 57 192 498 1401 Size: 57 135 306 903 Fragments arranged by size: 903 306 135 57 BmrI ACTGGGnnnn_n' Cuts at: 0 333 1401 Size: 333 1068 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1347 1401 Size: 1347 54 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 367 685 862 1047 1401 Size: 151 216 318 177 185 354 Fragments arranged by size: 354 318 216 185 177 151 Bpu10I CC'TnA_GC Cuts at: 0 589 1174 1401 Size: 589 585 227 Fragments arranged by size: 589 585 227 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1231 1401 Size: 1231 170 BsaI GGTCTCn'nnnn_ Cuts at: 0 661 1401 Size: 661 740 BsaAI yAC'GTr Cuts at: 0 792 894 1401 Size: 792 102 507 Fragments arranged by size: 792 507 102 BsaHI Gr'CG_yC Cuts at: 0 486 1401 Size: 486 915 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 658 688 1401 Size: 658 30 713 Fragments arranged by size: 713 658 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 397 400 631 634 1401 Size: 397 3 231 3 767 Fragments arranged by size: 767 397 231 3 3 BseYI C'CCAG_C Cuts at: 0 804 918 933 1041 1401 Size: 804 114 15 108 360 Fragments arranged by size: 804 360 114 108 15 BsiEI CG_ry'CG Cuts at: 0 219 395 480 575 1401 Size: 219 176 85 95 826 Fragments arranged by size: 826 219 176 95 85 BsiHKAI G_wGCw'C Cuts at: 0 595 637 890 1025 1247 1401 Size: 595 42 253 135 222 154 Fragments arranged by size: 595 253 222 154 135 42 Bsp1286I G_dGCh'C Cuts at: 0 289 439 595 637 890 1025 1073 1247 Size: 289 150 156 42 253 135 48 174 Cuts at: 1247 1342 1401 Size: 95 59 Fragments arranged by size: 289 253 174 156 150 135 95 59 48 42 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 173 1140 1401 Size: 173 967 261 Fragments arranged by size: 967 261 173 BsrBI CCG'CTC Cuts at: 0 624 1401 Size: 624 777 BsrFI r'CCGG_y Cuts at: 0 479 1281 1401 Size: 479 802 120 Fragments arranged by size: 802 479 120 BssHII G'CGCG_C Cuts at: 0 124 141 258 293 340 442 444 744 Size: 124 17 117 35 47 102 2 300 Cuts at: 744 746 846 907 956 958 1046 1048 1263 Size: 2 100 61 49 2 88 2 215 Cuts at: 1263 1401 Size: 138 Fragments arranged by size: 300 215 138 124 117 102 100 88 61 49 47 35 17 2 2 2 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 227 1401 Size: 227 1174 BstEII G'GTnAC_C Cuts at: 0 1203 1401 Size: 1203 198 BstXI CCAn_nnnn'nTGG Cuts at: 0 840 1401 Size: 840 561 BtgI C'CryG_G Cuts at: 0 7 150 575 998 1109 1370 1401 Size: 7 143 425 423 111 261 31 Fragments arranged by size: 425 423 261 143 111 31 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 126 1401 Size: 126 1275 EaeI y'GGCC_r Cuts at: 0 153 392 477 563 572 995 1028 1268 Size: 153 239 85 86 9 423 33 240 Cuts at: 1268 1310 1358 1401 Size: 42 48 43 Fragments arranged by size: 423 240 239 153 86 85 48 43 42 33 9 EagI C'GGCC_G Cuts at: 0 392 477 572 1401 Size: 392 85 95 829 Fragments arranged by size: 829 392 95 85 EarI CTCTTCn'nnn_ Cuts at: 0 541 1012 1401 Size: 541 471 389 Fragments arranged by size: 541 471 389 EcoICRI GAG'CTC Cuts at: 0 1245 1401 Size: 1245 156 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 367 685 862 1047 1401 Size: 151 216 318 177 185 354 Fragments arranged by size: 354 318 216 185 177 151 EcoNI CCTnn'n_nnAGG Cuts at: 0 1291 1401 Size: 1291 110 EcoO109I rG'GnC_Cy Cuts at: 0 41 285 454 1338 1339 1401 Size: 41 244 169 884 1 62 Fragments arranged by size: 884 244 169 62 41 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 753 1401 Size: 753 648 HaeII r_GCGC'y Cuts at: 0 138 253 354 489 778 1006 1162 1401 Size: 138 115 101 135 289 228 156 239 Fragments arranged by size: 289 239 228 156 138 135 115 101 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 104 136 1401 Size: 104 32 1265 Fragments arranged by size: 1265 104 32 KasI G'GCGC_C Cuts at: 0 485 1401 Size: 485 916 MscI TGG'CCA Cuts at: 0 997 1401 Size: 997 404 MslI CAynn'nnrTG Cuts at: 0 67 574 1099 1401 Size: 67 507 525 302 Fragments arranged by size: 525 507 302 67 MspA1I CmG'CkG Cuts at: 0 152 953 1178 1372 1401 Size: 152 801 225 194 29 Fragments arranged by size: 801 225 194 152 29 NaeI GCC'GGC Cuts at: 0 481 1283 1401 Size: 481 802 118 Fragments arranged by size: 802 481 118 NarI GG'CG_CC Cuts at: 0 486 1401 Size: 486 915 NcoI C'CATG_G Cuts at: 0 7 998 1401 Size: 7 991 403 Fragments arranged by size: 991 403 7 NgoMIV G'CCGG_C Cuts at: 0 479 1281 1401 Size: 479 802 120 Fragments arranged by size: 802 479 120 PasI CC'CwG_GG Cuts at: 0 450 1401 Size: 450 951 PflMI CCAn_nnn'nTGG Cuts at: 0 918 1082 1401 Size: 918 164 319 Fragments arranged by size: 918 319 164 PfoI T'CCnGG_A Cuts at: 0 839 1401 Size: 839 562 PpuMI rG'GwC_Cy Cuts at: 0 41 454 1401 Size: 41 413 947 Fragments arranged by size: 947 413 41 PspOMI G'GGCC_C Cuts at: 0 285 1338 1401 Size: 285 1053 63 Fragments arranged by size: 1053 285 63 PstI C_TGCA'G Cuts at: 0 473 719 1292 1401 Size: 473 246 573 109 Fragments arranged by size: 573 473 246 109 SacI G_AGCT'C Cuts at: 0 1247 1401 Size: 1247 154 SacII CC_GC'GG Cuts at: 0 153 1373 1401 Size: 153 1220 28 Fragments arranged by size: 1220 153 28 SanDI GG'GwC_CC Cuts at: 0 41 1401 Size: 41 1360 SapI GCTCTTCn'nnn_ Cuts at: 0 541 1012 1401 Size: 541 471 389 Fragments arranged by size: 541 471 389 SbfI CC_TGCA'GG Cuts at: 0 719 1292 1401 Size: 719 573 109 Fragments arranged by size: 719 573 109 SfcI C'TryA_G Cuts at: 0 469 715 1288 1401 Size: 469 246 573 113 Fragments arranged by size: 573 469 246 113 SfiI GGCCn_nnn'nGGCC Cuts at: 0 571 1401 Size: 571 830 SfoI GGC'GCC Cuts at: 0 487 1401 Size: 487 914 SmaI CCC'GGG Cuts at: 0 23 824 1146 1401 Size: 23 801 322 255 Fragments arranged by size: 801 322 255 23 SmlI C'TyrA_G Cuts at: 0 1246 1401 Size: 1246 155 SrfI GCCC'GGGC Cuts at: 0 824 1146 1401 Size: 824 322 255 Fragments arranged by size: 824 322 255 StyI C'CwwG_G Cuts at: 0 7 998 1401 Size: 7 991 403 Fragments arranged by size: 991 403 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1374 1401 Size: 1374 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 658 690 1366 1398 1401 Size: 658 32 676 32 3 Fragments arranged by size: 676 658 32 32 3 Tth111I GACn'n_nGTC Cuts at: 0 1126 1401 Size: 1126 275 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1107 1401 Size: 1107 294 XmaI C'CCGG_G Cuts at: 0 21 822 1144 1401 Size: 21 801 322 257 Fragments arranged by size: 801 322 257 21 Enzymes that do cut: AarI AfeI AflIII AleI ApaI ApaLI AscI AvaI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI BtgI BtgZI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI SacI SacII SanDI SapI SbfI SfcI SfiI SfoI SmaI SmlI SrfI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AgeI AhdI AlfI AloI AlwNI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII BmtI BsaBI BsaWI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TatI XbaI XhoI XmnI ZraI