Restriction map for
pFN21AE5021
(Linear) (Six-base) MAPSORT of: KIEE5021.seq Check: 5587 from: 1 to: 1290
KIEE5021.orf
With 182 enzymes: SgfI *
December 5, 2014 12:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 900 1290
Size: 900 390
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 744 1290
Size: 219 525 546
Fragments arranged by size:
546 525 219
AfeI AGC'GCT
Cuts at: 0 240 1290
Size: 240 1050
AleI CACnn'nnGTG
Cuts at: 0 376 709 1290
Size: 376 333 581
Fragments arranged by size:
581 376 333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 580 1290
Size: 580 710
AscI GG'CGCG_CC
Cuts at: 0 977 1290
Size: 977 313
AvaI C'yCGr_G
Cuts at: 0 178 1092 1227 1290
Size: 178 914 135 63
Fragments arranged by size:
914 178 135 63
BanI G'GyrC_C
Cuts at: 0 157 217 352 370 1045 1290
Size: 157 60 135 18 675 245
Fragments arranged by size:
675 245 157 135 60 18
BanII G_rGCy'C
Cuts at: 0 187 824 1103 1290
Size: 187 637 279 187
Fragments arranged by size:
637 279 187 187
BbeI G_GCGC'C
Cuts at: 0 221 356 1290
Size: 221 135 934
Fragments arranged by size:
934 221 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 159 1290
Size: 159 1131
BglI GCCn_nnn'nGGC
Cuts at: 0 301 560 620 1094 1220 1290
Size: 301 259 60 474 126 70
Fragments arranged by size:
474 301 259 126 70 60
BlpI GC'TnA_GC
Cuts at: 0 937 1290
Size: 937 353
Bme1580I G_kGCm'C
Cuts at: 0 373 1290
Size: 373 917
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1122 1290
Size: 1122 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1269 1290
Size: 514 755 21
Fragments arranged by size:
755 514 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 1267 1290
Size: 1267 23
BsaAI yAC'GTr
Cuts at: 0 645 729 1290
Size: 645 84 561
Fragments arranged by size:
645 561 84
BsaHI Gr'CG_yC
Cuts at: 0 218 311 353 1073 1290
Size: 218 93 42 720 217
Fragments arranged by size:
720 218 217 93 42
BsaWI w'CCGG_w
Cuts at: 0 809 1290
Size: 809 481
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 877 907 1290
Size: 877 30 383
Fragments arranged by size:
877 383 30
BseYI C'CCAG_C
Cuts at: 0 212 338 463 1245 1290
Size: 212 126 125 782 45
Fragments arranged by size:
782 212 126 125 45
BsiEI CG_ry'CG
Cuts at: 0 111 1290
Size: 111 1179
BsiHKAI G_wGCw'C
Cuts at: 0 917 1103 1290
Size: 917 186 187
Fragments arranged by size:
917 187 186
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1165 1290
Size: 1165 125
Bsp1286I G_dGCh'C
Cuts at: 0 187 373 824 917 1103 1290
Size: 187 186 451 93 186 187
Fragments arranged by size:
451 187 187 186 186 93
BspHI T'CATG_A
Cuts at: 0 1253 1290
Size: 1253 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 900 1290
Size: 900 390
BsrDI GCAATG_nn'
Cuts at: 0 1002 1290
Size: 1002 288
BsrFI r'CCGG_y
Cuts at: 0 106 307 1262 1290
Size: 106 201 955 28
Fragments arranged by size:
955 201 106 28
BsrGI T'GTAC_A
Cuts at: 0 701 1290
Size: 701 589
BssHII G'CGCG_C
Cuts at: 0 208 270 528 953 955 977 1241 1290
Size: 208 62 258 425 2 22 264 49
Fragments arranged by size:
425 264 258 208 62 49 22 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1199 1290
Size: 1199 91
BtgI C'CryG_G
Cuts at: 0 374 585 1055 1290
Size: 374 211 470 235
Fragments arranged by size:
470 374 235 211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 423 1290
Size: 423 867
DraIII CAC_nnn'GTG
Cuts at: 0 252 1290
Size: 252 1038
EaeI y'GGCC_r
Cuts at: 0 108 254 302 695 1034 1175 1268 1290
Size: 108 146 48 393 339 141 93 22
Fragments arranged by size:
393 339 146 141 108 93 48 22
EagI C'GGCC_G
Cuts at: 0 108 1290
Size: 108 1182
EarI CTCTTCn'nnn_
Cuts at: 0 493 850 886 1290
Size: 493 357 36 404
Fragments arranged by size:
493 404 357 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 211 571 1290
Size: 211 360 719
Fragments arranged by size:
719 360 211
EcoICRI GAG'CTC
Cuts at: 0 1101 1290
Size: 1101 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 514 744 1269 1290
Size: 219 295 230 525 21
Fragments arranged by size:
525 295 230 219 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 97 1290
Size: 97 1193
EcoO109I rG'GnC_Cy
Cuts at: 0 780 1290
Size: 780 510
FspI TGC'GCA
Cuts at: 0 229 245 931 1290
Size: 229 16 686 359
Fragments arranged by size:
686 359 229 16
HaeII r_GCGC'y
Cuts at: 0 133 178 221 242 356 546 1150 1283
Size: 133 45 43 21 114 190 604 133
Cuts at: 1283 1290
Size: 7
Fragments arranged by size:
604 190 133 133 114 45 43 21
7
HincII GTy'rAC
Cuts at: 0 383 1206 1290
Size: 383 823 84
Fragments arranged by size:
823 383 84
KasI G'GCGC_C
Cuts at: 0 217 352 1290
Size: 217 135 938
Fragments arranged by size:
938 217 135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1154 1290
Size: 1148 6 136
Fragments arranged by size:
1148 136 6
MslI CAynn'nnrTG
Cuts at: 0 376 709 1108 1290
Size: 376 333 399 182
Fragments arranged by size:
399 376 333 182
MspA1I CmG'CkG
Cuts at: 0 549 560 587 947 1057 1164 1290
Size: 549 11 27 360 110 107 126
Fragments arranged by size:
549 360 126 110 107 27 11
NaeI GCC'GGC
Cuts at: 0 309 1290
Size: 309 981
NarI GG'CG_CC
Cuts at: 0 218 353 1290
Size: 218 135 937
Fragments arranged by size:
937 218 135
NgoMIV G'CCGG_C
Cuts at: 0 307 1290
Size: 307 983
PfoI T'CCnGG_A
Cuts at: 0 491 1290
Size: 491 799
PmlI CAC'GTG
Cuts at: 0 645 1290
Size: 645 645
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1210 1242 1290
Size: 1210 32 48
Fragments arranged by size:
1210 48 32
PpuMI rG'GwC_Cy
Cuts at: 0 780 1290
Size: 780 510
PstI C_TGCA'G
Cuts at: 0 1157 1290
Size: 1157 133
PvuII CAG'CTG
Cuts at: 0 549 1164 1290
Size: 549 615 126
Fragments arranged by size:
615 549 126
SacI G_AGCT'C
Cuts at: 0 1103 1290
Size: 1103 187
SacII CC_GC'GG
Cuts at: 0 588 1058 1290
Size: 588 470 232
Fragments arranged by size:
588 470 232
SapI GCTCTTCn'nnn_
Cuts at: 0 886 1290
Size: 886 404
SexAI A'CCwGG_T
Cuts at: 0 146 782 1290
Size: 146 636 508
Fragments arranged by size:
636 508 146
SfcI C'TryA_G
Cuts at: 0 1153 1290
Size: 1153 137
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 301 1290
Size: 301 989
SfoI GGC'GCC
Cuts at: 0 219 354 1290
Size: 219 135 936
Fragments arranged by size:
936 219 135
SgrAI Cr'CCGG_yG
Cuts at: 0 307 1290
Size: 307 983
SmlI C'TyrA_G
Cuts at: 0 178 1290
Size: 178 1112
StuI AGG'CCT
Cuts at: 0 430 1290
Size: 430 860
TatI w'GTAC_w
Cuts at: 0 701 1290
Size: 701 589
XhoI C'TCGA_G
Cuts at: 0 178 1290
Size: 178 1112
XmnI GAAnn'nnTTC
Cuts at: 0 430 1222 1290
Size: 430 792 68
Fragments arranged by size:
792 430 68
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AscI AvaI BanI
BanII BbeI BbsI BglI BlpI Bme1580I BplI BpmI
BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssHII
BstAPI BtgI BtgZI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII HincII KasI
MmeI MslI MspA1I NaeI NarI NgoMIV PfoI PmlI
PpiI PpuMI PstI PvuII SacI SacII SapI SexAI
SfcI SfiI SfoI SgrAI SmlI StuI TatI XhoI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmrI BmtI Bpu10I BpuEI BsaBI BseRI BsgI
BsiWI BsmI BspEI BsrBI BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI
KpnI MfeI MluI MscI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SanDI SbfI ScaI SgfI SmaI SnaBI SpeI SphI
SrfI SspI StyI SwaI TaqII TstI Tth111I XbaI
XcmI XmaI ZraI