Restriction map for
pFN21AE5016
(Linear) (Six-base) MAPSORT of: KIEE5016.seq Check: 4415 from: 1 to: 1002
KIEE5016.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 599 1002
Size: 599 403
AleI CACnn'nnGTG
Cuts at: 0 145 953 1002
Size: 145 808 49
Fragments arranged by size:
808 145 49
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 1002
Size: 163 839
AlwNI CAG_nnn'CTG
Cuts at: 0 297 369 1002
Size: 297 72 633
Fragments arranged by size:
633 297 72
ApaI G_GGCC'C
Cuts at: 0 10 78 1002
Size: 10 68 924
Fragments arranged by size:
924 68 10
ApaLI G'TGCA_C
Cuts at: 0 138 1002
Size: 138 864
AscI GG'CGCG_CC
Cuts at: 0 466 1002
Size: 466 536
AvaI C'yCGr_G
Cuts at: 0 102 130 198 802 972 1002
Size: 102 28 68 604 170 30
Fragments arranged by size:
604 170 102 68 30 28
BanI G'GyrC_C
Cuts at: 0 115 265 402 653 1002
Size: 115 150 137 251 349
Fragments arranged by size:
349 251 150 137 115
BanII G_rGCy'C
Cuts at: 0 10 78 479 853 860 982 1002
Size: 10 68 401 374 7 122 20
Fragments arranged by size:
401 374 122 68 20 10 7
BbeI G_GCGC'C
Cuts at: 0 119 1002
Size: 119 883
BlpI GC'TnA_GC
Cuts at: 0 715 1002
Size: 715 287
Bme1580I G_kGCm'C
Cuts at: 0 10 78 142 197 1002
Size: 10 68 64 55 805
Fragments arranged by size:
805 68 64 55 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1002
Size: 493 509
Bpu10I CC'TnA_GC
Cuts at: 0 205 376 1002
Size: 205 171 626
Fragments arranged by size:
626 205 171
BsaI GGTCTCn'nnnn_
Cuts at: 0 909 1002
Size: 909 93
BsaAI yAC'GTr
Cuts at: 0 143 1002
Size: 143 859
BsaHI Gr'CG_yC
Cuts at: 0 116 705 740 1002
Size: 116 589 35 262
Fragments arranged by size:
589 262 116 35
BseYI C'CCAG_C
Cuts at: 0 558 846 1002
Size: 558 288 156
Fragments arranged by size:
558 288 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 571 1002
Size: 304 267 431
Fragments arranged by size:
431 304 267
BsiEI CG_ry'CG
Cuts at: 0 31 1002
Size: 31 971
BsiHKAI G_wGCw'C
Cuts at: 0 142 689 1002
Size: 142 547 313
Fragments arranged by size:
547 313 142
BsmBI CGTCTCn'nnnn_
Cuts at: 0 673 1002
Size: 673 329
Bsp1286I G_dGCh'C
Cuts at: 0 10 78 142 197 479 689 853 860
Size: 10 68 64 55 282 210 164 7
Cuts at: 860 982 1002
Size: 122 20
Fragments arranged by size:
282 210 164 122 68 64 55 20
10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 311 599 1002
Size: 311 288 403
Fragments arranged by size:
403 311 288
BsrBI CCG'CTC
Cuts at: 0 280 574 633 768 1002
Size: 280 294 59 135 234
Fragments arranged by size:
294 280 234 135 59
BsrDI GCAATG_nn'
Cuts at: 0 753 1002
Size: 753 249
BsrFI r'CCGG_y
Cuts at: 0 451 458 650 807 1002
Size: 451 7 192 157 195
Fragments arranged by size:
451 195 192 157 7
BssHII G'CGCG_C
Cuts at: 0 219 221 284 356 426 428 466 491
Size: 219 2 63 72 70 2 38 25
Cuts at: 491 620 758 874 1002
Size: 129 138 116 128
Fragments arranged by size:
219 138 129 128 116 72 70 63
38 25 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 327 660 1002
Size: 327 333 342
Fragments arranged by size:
342 333 327
BstXI CCAn_nnnn'nTGG
Cuts at: 0 390 1002
Size: 390 612
BtgI C'CryG_G
Cuts at: 0 89 479 788 948 965 1002
Size: 89 390 309 160 17 37
Fragments arranged by size:
390 309 160 89 37 17
DraIII CAC_nnn'GTG
Cuts at: 0 327 1002
Size: 327 675
EaeI y'GGCC_r
Cuts at: 0 28 86 161 449 697 785 1002
Size: 28 58 75 288 248 88 217
Fragments arranged by size:
288 248 217 88 75 58 28
EagI C'GGCC_G
Cuts at: 0 28 1002
Size: 28 974
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 895 1002
Size: 895 107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 1002
Size: 493 509
EcoO109I rG'GnC_Cy
Cuts at: 0 6 7 107 348 780 1002
Size: 6 1 100 241 432 222
Fragments arranged by size:
432 241 222 100 6 1
FseI GG_CCGG'CC
Cuts at: 0 811 1002
Size: 811 191
FspI TGC'GCA
Cuts at: 0 436 1002
Size: 436 566
HaeII r_GCGC'y
Cuts at: 0 119 297 383 487 847 1002
Size: 119 178 86 104 360 155
Fragments arranged by size:
360 178 155 119 104 86
KasI G'GCGC_C
Cuts at: 0 115 1002
Size: 115 887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 1002
Size: 761 241
MscI TGG'CCA
Cuts at: 0 163 1002
Size: 163 839
MslI CAynn'nnrTG
Cuts at: 0 145 953 1002
Size: 145 808 49
Fragments arranged by size:
808 145 49
MspA1I CmG'CkG
Cuts at: 0 33 167 366 390 423 481 501 660
Size: 33 134 199 24 33 58 20 159
Cuts at: 660 967 988 1002
Size: 307 21 14
Fragments arranged by size:
307 199 159 134 58 33 33 24
21 20 14
NaeI GCC'GGC
Cuts at: 0 453 652 809 1002
Size: 453 199 157 193
Fragments arranged by size:
453 199 193 157
NarI GG'CG_CC
Cuts at: 0 116 1002
Size: 116 886
NgoMIV G'CCGG_C
Cuts at: 0 451 650 807 1002
Size: 451 199 157 195
Fragments arranged by size:
451 199 195 157
NspI r_CATG'y
Cuts at: 0 334 1002
Size: 334 668
PasI CC'CwG_GG
Cuts at: 0 111 1002
Size: 111 891
PmlI CAC'GTG
Cuts at: 0 143 1002
Size: 143 859
PpuMI rG'GwC_Cy
Cuts at: 0 107 780 1002
Size: 107 673 222
Fragments arranged by size:
673 222 107
PspOMI G'GGCC_C
Cuts at: 0 6 74 1002
Size: 6 68 928
Fragments arranged by size:
928 68 6
PstI C_TGCA'G
Cuts at: 0 365 524 1002
Size: 365 159 478
Fragments arranged by size:
478 365 159
PvuII CAG'CTG
Cuts at: 0 167 366 390 1002
Size: 167 199 24 612
Fragments arranged by size:
612 199 167 24
SacII CC_GC'GG
Cuts at: 0 482 968 1002
Size: 482 486 34
Fragments arranged by size:
486 482 34
SanDI GG'GwC_CC
Cuts at: 0 107 1002
Size: 107 895
SbfI CC_TGCA'GG
Cuts at: 0 524 1002
Size: 524 478
SfcI C'TryA_G
Cuts at: 0 289 361 520 1002
Size: 289 72 159 482
Fragments arranged by size:
482 289 159 72
SfoI GGC'GCC
Cuts at: 0 117 1002
Size: 117 885
SmaI CCC'GGG
Cuts at: 0 200 974 1002
Size: 200 774 28
Fragments arranged by size:
774 200 28
StuI AGG'CCT
Cuts at: 0 158 1002
Size: 158 844
StyI C'CwwG_G
Cuts at: 0 61 604 1002
Size: 61 543 398
Fragments arranged by size:
543 398 61
XbaI T'CTAG_A
Cuts at: 0 576 1002
Size: 576 426
XmaI C'CCGG_G
Cuts at: 0 198 972 1002
Size: 198 774 30
Fragments arranged by size:
774 198 30
Enzymes that do cut:
AarI AleI AlfI AlwNI ApaI ApaLI AscI AvaI
BanI BanII BbeI BlpI Bme1580I BpmI Bpu10I BsaI
BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BtgI
DraIII EaeI EagI EciI Eco57MI EcoO109I FseI FspI
HaeII KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PmlI PpuMI PspOMI PstI PvuII
SacII SanDI SbfI SfcI SfoI SmaI StuI StyI
XbaI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AloI ApoI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BmtI BplI BpuEI BsaBI BsaWI
BsaXI BseRI BsiWI BsmI BspEI BspHI BsrGI BssSI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EarI EcoICRI EcoNI EcoRI EcoRV
FalI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SapI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI Tth111I XcmI XhoI
XmnI ZraI