Restriction map for
pFN21AE5013
(Linear) (Six-base) MAPSORT of: KIEE5013.seq Check: 6607 from: 1 to: 810
KIEE5013.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 656 810
Size: 656 154
AlwNI CAG_nnn'CTG
Cuts at: 0 388 798 810
Size: 388 410 12
Fragments arranged by size:
410 388 12
ApaI G_GGCC'C
Cuts at: 0 505 606 810
Size: 505 101 204
Fragments arranged by size:
505 204 101
ApoI r'AATT_y
Cuts at: 0 658 673 810
Size: 658 15 137
Fragments arranged by size:
658 137 15
AvaI C'yCGr_G
Cuts at: 0 361 594 810
Size: 361 233 216
Fragments arranged by size:
361 233 216
BanI G'GyrC_C
Cuts at: 0 193 810
Size: 193 617
BanII G_rGCy'C
Cuts at: 0 40 445 468 505 606 810
Size: 40 405 23 37 101 204
Fragments arranged by size:
405 204 101 40 37 23
BbeI G_GCGC'C
Cuts at: 0 197 810
Size: 197 613
BbvCI CC'TCA_GC
Cuts at: 0 721 810
Size: 721 89
Bme1580I G_kGCm'C
Cuts at: 0 505 606 810
Size: 505 101 204
Fragments arranged by size:
505 204 101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 810
Size: 442 368
Bpu10I CC'TnA_GC
Cuts at: 0 721 810
Size: 721 89
BsaHI Gr'CG_yC
Cuts at: 0 194 810
Size: 194 616
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 810
Size: 343 467
BseYI C'CCAG_C
Cuts at: 0 319 810
Size: 319 491
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 810
Size: 547 263
BsiEI CG_ry'CG
Cuts at: 0 317 339 349 810
Size: 317 22 10 461
Fragments arranged by size:
461 317 22 10
Bsp1286I G_dGCh'C
Cuts at: 0 40 445 468 505 606 810
Size: 40 405 23 37 101 204
Fragments arranged by size:
405 204 101 40 37 23
BsrBI CCG'CTC
Cuts at: 0 25 507 810
Size: 25 482 303
Fragments arranged by size:
482 303 25
BssHII G'CGCG_C
Cuts at: 0 140 810
Size: 140 670
BstAPI GCAn_nnn'nTGC
Cuts at: 0 388 810
Size: 388 422
Bsu36I CC'TnA_GG
Cuts at: 0 85 810
Size: 85 725
BtgI C'CryG_G
Cuts at: 0 580 810
Size: 580 230
BtsI GCAGTG_nn'
Cuts at: 0 197 389 562 810
Size: 197 192 173 248
Fragments arranged by size:
248 197 192 173
DrdI GACnn_nn'nnGTC
Cuts at: 0 733 810
Size: 733 77
EaeI y'GGCC_r
Cuts at: 0 99 107 314 346 401 810
Size: 99 8 207 32 55 409
Fragments arranged by size:
409 207 99 55 32 8
EagI C'GGCC_G
Cuts at: 0 314 346 810
Size: 314 32 464
Fragments arranged by size:
464 314 32
EarI CTCTTCn'nnn_
Cuts at: 0 278 810
Size: 278 532
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 810
Size: 442 368
EcoNI CCTnn'n_nnAGG
Cuts at: 0 457 810
Size: 457 353
EcoO109I rG'GnC_Cy
Cuts at: 0 501 602 810
Size: 501 101 208
Fragments arranged by size:
501 208 101
EcoRI G'AATT_C
Cuts at: 0 658 673 810
Size: 658 15 137
Fragments arranged by size:
658 137 15
HaeII r_GCGC'y
Cuts at: 0 197 799 805 810
Size: 197 602 6 5
Fragments arranged by size:
602 197 6 5
HincII GTy'rAC
Cuts at: 0 669 810
Size: 669 141
KasI G'GCGC_C
Cuts at: 0 193 810
Size: 193 617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 629 810
Size: 104 525 181
Fragments arranged by size:
525 181 104
MslI CAynn'nnrTG
Cuts at: 0 523 810
Size: 523 287
MspA1I CmG'CkG
Cuts at: 0 319 810
Size: 319 491
NarI GG'CG_CC
Cuts at: 0 194 810
Size: 194 616
NotI GC'GGCC_GC
Cuts at: 0 314 810
Size: 314 496
PasI CC'CwG_GG
Cuts at: 0 149 810
Size: 149 661
PspOMI G'GGCC_C
Cuts at: 0 501 602 810
Size: 501 101 208
Fragments arranged by size:
501 208 101
PstI C_TGCA'G
Cuts at: 0 56 257 384 810
Size: 56 201 127 426
Fragments arranged by size:
426 201 127 56
SapI GCTCTTCn'nnn_
Cuts at: 0 278 810
Size: 278 532
SfcI C'TryA_G
Cuts at: 0 18 52 253 380 810
Size: 18 34 201 127 430
Fragments arranged by size:
430 201 127 34 18
SfoI GGC'GCC
Cuts at: 0 195 810
Size: 195 615
SmaI CCC'GGG
Cuts at: 0 596 810
Size: 596 214
StyI C'CwwG_G
Cuts at: 0 497 810
Size: 497 313
XmaI C'CCGG_G
Cuts at: 0 594 810
Size: 594 216
XmnI GAAnn'nnTTC
Cuts at: 0 658 810
Size: 658 152
Enzymes that do cut:
AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BbvCI Bme1580I BpmI Bpu10I BsaHI BseRI BseYI BsgI
BsiEI Bsp1286I BsrBI BssHII BstAPI Bsu36I BtgI BtsI
DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI
HaeII HincII KasI MmeI MslI MspA1I NarI NotI
PasI PspOMI PstI SapI SfcI SfoI SmaI StyI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII EciI EcoICRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
MscI NaeI NcoI NdeI NgoMIV NheI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI ZraI