Restriction map for
pFN21AE5012
(Linear) (Six-base) MAPSORT of: KIEE5012.seq Check: 7205 from: 1 to: 3003
KIEE5012.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AclI AA'CG_TT
Cuts at: 0 1374 3003
Size: 1374 1629
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 770 1111 1698 1719 1743 1767 1815
Size: 605 165 341 587 21 24 24 48
Cuts at: 1815 1911 1935 2044 2079 2127 2271 2415 2463
Size: 96 24 109 35 48 144 144 48
Cuts at: 2463 2511 3003
Size: 48 492
Fragments arranged by size:
605 587 492 341 165 144 144 109
96 48 48 48 48 35 24 24
24 21
AflII C'TTAA_G
Cuts at: 0 1974 3003
Size: 1974 1029
AflIII A'CryG_T
Cuts at: 0 513 826 1152 3003
Size: 513 313 326 1851
Fragments arranged by size:
1851 513 326 313
AhdI GACnn_n'nnGTC
Cuts at: 0 315 3003
Size: 315 2688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 871 1681 1713 3003
Size: 839 32 810 32 1290
Fragments arranged by size:
1290 839 810 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 292 1197 3003
Size: 292 905 1806
Fragments arranged by size:
1806 905 292
ApaI G_GGCC'C
Cuts at: 0 2440 2978 3003
Size: 2440 538 25
Fragments arranged by size:
2440 538 25
ApoI r'AATT_y
Cuts at: 0 598 903 1215 1339 1455 2658 2819 3003
Size: 598 305 312 124 116 1203 161 184
Fragments arranged by size:
1203 598 312 305 184 161 124 116
AseI AT'TA_AT
Cuts at: 0 377 776 3003
Size: 377 399 2227
Fragments arranged by size:
2227 399 377
AvaI C'yCGr_G
Cuts at: 0 1939 2083 2393 3003
Size: 1939 144 310 610
Fragments arranged by size:
1939 610 310 144
AvrII C'CTAG_G
Cuts at: 0 1395 1744 1840 3003
Size: 1395 349 96 1163
Fragments arranged by size:
1395 1163 349 96
BanI G'GyrC_C
Cuts at: 0 1655 1787 1811 1835 2315 2411 2459 3003
Size: 1655 132 24 24 480 96 48 544
Fragments arranged by size:
1655 544 480 132 96 48 24 24
BanII G_rGCy'C
Cuts at: 0 212 2440 2488 2623 2978 3003
Size: 212 2228 48 135 355 25
Fragments arranged by size:
2228 355 212 135 48 25
BbeI G_GCGC'C
Cuts at: 0 1791 1815 2319 3003
Size: 1791 24 504 684
Fragments arranged by size:
1791 684 504 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 247 1673 1760 2432 2456 2552 3003
Size: 247 1426 87 672 24 96 451
Fragments arranged by size:
1426 672 451 247 96 87 24
BbvCI CC'TCA_GC
Cuts at: 0 1277 3003
Size: 1277 1726
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2176 2210 3003
Size: 2176 34 793
Fragments arranged by size:
2176 793 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1653 2973 3003
Size: 1653 1320 30
Fragments arranged by size:
1653 1320 30
BglII A'GATC_T
Cuts at: 0 745 2952 3003
Size: 745 2207 51
Fragments arranged by size:
2207 745 51
Bme1580I G_kGCm'C
Cuts at: 0 2440 2978 3003
Size: 2440 538 25
Fragments arranged by size:
2440 538 25
BmgBI CAC'GTC
Cuts at: 0 163 3003
Size: 163 2840
BmrI ACTGGGnnnn_n'
Cuts at: 0 31 3003
Size: 31 2972
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2195 2243 3003
Size: 2195 48 760
Fragments arranged by size:
2195 760 48
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1646 1875 1899 1912 2043 2213 3003
Size: 1646 229 24 13 131 170 790
Fragments arranged by size:
1646 790 229 170 131 24 13
Bpu10I CC'TnA_GC
Cuts at: 0 1277 3003
Size: 1277 1726
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1157 1620 1888 3003
Size: 1157 463 268 1115
Fragments arranged by size:
1157 1115 463 268
BsaI GGTCTCn'nnnn_
Cuts at: 0 806 1916 2214 2334 3003
Size: 806 1110 298 120 669
Fragments arranged by size:
1110 806 669 298 120
BsaAI yAC'GTr
Cuts at: 0 514 1504 3003
Size: 514 990 1499
Fragments arranged by size:
1499 990 514
BsaHI Gr'CG_yC
Cuts at: 0 282 1788 1812 2316 3003
Size: 282 1506 24 504 687
Fragments arranged by size:
1506 687 504 282 24
BsaWI w'CCGG_w
Cuts at: 0 1818 2168 2216 2336 2562 3003
Size: 1818 350 48 120 226 441
Fragments arranged by size:
1818 441 350 226 120 48
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 107 1671 1789 1861 1872 1885 1909 1957
Size: 107 1564 118 72 11 13 24 48
Cuts at: 1957 1981 2029 2053 2125 2149 2581 3003
Size: 24 48 24 72 24 432 422
Fragments arranged by size:
1564 432 422 118 107 72 72 48
48 24 24 24 24 13 11
BseYI C'CCAG_C
Cuts at: 0 388 3003
Size: 388 2615
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 3003
Size: 1267 1736
BsiEI CG_ry'CG
Cuts at: 0 2368 3003
Size: 2368 635
BsiHKAI G_wGCw'C
Cuts at: 0 212 2261 2285 2333 3003
Size: 212 2049 24 48 670
Fragments arranged by size:
2049 670 212 48 24
BsmI GAATG_Cn'
Cuts at: 0 2124 3003
Size: 2124 879
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2019 3003
Size: 2019 984
Bsp1286I G_dGCh'C
Cuts at: 0 212 2261 2285 2333 2440 2488 2623 2978
Size: 212 2049 24 48 107 48 135 355
Cuts at: 2978 3003
Size: 25
Fragments arranged by size:
2049 355 212 135 107 48 48 25
24
BspEI T'CCGG_A
Cuts at: 0 1818 2168 2216 2336 2562 3003
Size: 1818 350 48 120 226 441
Fragments arranged by size:
1818 441 350 226 120 48
BspHI T'CATG_A
Cuts at: 0 564 3003
Size: 564 2439
BsrBI CCG'CTC
Cuts at: 0 1018 3003
Size: 1018 1985
BsrDI GCAATG_nn'
Cuts at: 0 1097 3003
Size: 1097 1906
BstAPI GCAn_nnn'nTGC
Cuts at: 0 163 3003
Size: 163 2840
BstBI TT'CG_AA
Cuts at: 0 792 3003
Size: 792 2211
BstEII G'GTnAC_C
Cuts at: 0 1513 3003
Size: 1513 1490
BstYI r'GATC_y
Cuts at: 0 289 745 2952 3003
Size: 289 456 2207 51
Fragments arranged by size:
2207 456 289 51
Bsu36I CC'TnA_GG
Cuts at: 0 1964 2012 2132 2180 2372 2551 2720 3003
Size: 1964 48 120 48 192 179 169 283
Fragments arranged by size:
1964 283 192 179 169 120 48 48
BtgI C'CryG_G
Cuts at: 0 1916 2835 3003
Size: 1916 919 168
Fragments arranged by size:
1916 919 168
DraI TTT'AAA
Cuts at: 0 1344 2802 3003
Size: 1344 1458 201
Fragments arranged by size:
1458 1344 201
EaeI y'GGCC_r
Cuts at: 0 570 1188 1885 2005 2197 2269 2293 2365
Size: 570 618 697 120 192 72 24 72
Cuts at: 2365 3003
Size: 638
Fragments arranged by size:
697 638 618 570 192 120 72 72
24
EagI C'GGCC_G
Cuts at: 0 2365 3003
Size: 2365 638
EarI CTCTTCn'nnn_
Cuts at: 0 270 375 1111 2063 3003
Size: 270 105 736 952 940
Fragments arranged by size:
952 940 736 270 105
EcoICRI GAG'CTC
Cuts at: 0 210 3003
Size: 210 2793
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 770 1111 1646 1698 1719 1743 1767
Size: 605 165 341 535 52 21 24 24
Cuts at: 1767 1815 1875 1899 1911 1912 1935 2043 2044
Size: 48 60 24 12 1 23 108 1
Cuts at: 2044 2079 2127 2213 2271 2415 2463 2511 3003
Size: 35 48 86 58 144 48 48 492
Fragments arranged by size:
605 535 492 341 165 144 108 86
60 58 52 48 48 48 48 35
24 24 24 23 21 12 1 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 89 1870 3003
Size: 89 1781 1133
Fragments arranged by size:
1781 1133 89
EcoO109I rG'GnC_Cy
Cuts at: 0 43 1862 2436 2437 2571 2975 3003
Size: 43 1819 574 1 134 404 28
Fragments arranged by size:
1819 574 404 134 43 28 1
HaeII r_GCGC'y
Cuts at: 0 1791 1815 2319 2391 3003
Size: 1791 24 504 72 612
Fragments arranged by size:
1791 612 504 72 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 173 205 301 333 2775 2807 3003
Size: 173 32 96 32 2442 32 196
Fragments arranged by size:
2442 196 173 96 32 32 32
HincII GTy'rAC
Cuts at: 0 309 2993 3003
Size: 309 2684 10
Fragments arranged by size:
2684 309 10
HpaI GTT'AAC
Cuts at: 0 2993 3003
Size: 2993 10
KasI G'GCGC_C
Cuts at: 0 1787 1811 2315 3003
Size: 1787 24 504 688
Fragments arranged by size:
1787 688 504 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1698 3003
Size: 1698 1305
MscI TGG'CCA
Cuts at: 0 1190 3003
Size: 1190 1813
MslI CAynn'nnrTG
Cuts at: 0 401 566 3003
Size: 401 165 2437
Fragments arranged by size:
2437 401 165
NarI GG'CG_CC
Cuts at: 0 1788 1812 2316 3003
Size: 1788 24 504 687
Fragments arranged by size:
1788 687 504 24
NcoI C'CATG_G
Cuts at: 0 2835 3003
Size: 2835 168
NotI GC'GGCC_GC
Cuts at: 0 2365 3003
Size: 2365 638
NspI r_CATG'y
Cuts at: 0 1156 3003
Size: 1156 1847
PciI A'CATG_T
Cuts at: 0 1152 3003
Size: 1152 1851
PflMI CCAn_nnn'nTGG
Cuts at: 0 292 2677 3003
Size: 292 2385 326
Fragments arranged by size:
2385 326 292
PfoI T'CCnGG_A
Cuts at: 0 285 3003
Size: 285 2718
PmlI CAC'GTG
Cuts at: 0 1504 3003
Size: 1504 1499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 816 848 3003
Size: 816 32 2155
Fragments arranged by size:
2155 816 32
PpuMI rG'GwC_Cy
Cuts at: 0 2571 3003
Size: 2571 432
PspOMI G'GGCC_C
Cuts at: 0 2436 2974 3003
Size: 2436 538 29
Fragments arranged by size:
2436 538 29
SacI G_AGCT'C
Cuts at: 0 212 3003
Size: 212 2791
SapI GCTCTTCn'nnn_
Cuts at: 0 375 3003
Size: 375 2628
SfcI C'TryA_G
Cuts at: 0 5 203 245 2894 3003
Size: 5 198 42 2649 109
Fragments arranged by size:
2649 198 109 42 5
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2973 3003
Size: 2973 30
SfoI GGC'GCC
Cuts at: 0 1789 1813 2317 3003
Size: 1789 24 504 686
Fragments arranged by size:
1789 686 504 24
SmlI C'TyrA_G
Cuts at: 0 1172 1599 1867 1974 3003
Size: 1172 427 268 107 1029
Fragments arranged by size:
1172 1029 427 268 107
StyI C'CwwG_G
Cuts at: 0 1395 1744 1792 1840 2835 3003
Size: 1395 349 48 48 995 168
Fragments arranged by size:
1395 995 349 168 48 48
SwaI ATTT'AAAT
Cuts at: 0 1344 3003
Size: 1344 1659
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2429 3003
Size: 2429 574
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1963 1995 3003
Size: 1963 32 1008
Fragments arranged by size:
1963 1008 32
XbaI T'CTAG_A
Cuts at: 0 1075 3003
Size: 1075 1928
XmnI GAAnn'nnTTC
Cuts at: 0 828 3003
Size: 828 2175
Enzymes that do cut:
AclI AcuI AflII AflIII AhdI AloI AlwNI ApaI
ApoI AseI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrDI BstAPI BstBI BstEII BstYI Bsu36I
BtgI DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII HpaI KasI MmeI MscI
MslI NarI NcoI NotI NspI PciI PflMI PfoI
PmlI PpiI PpuMI PspOMI SacI SapI SfcI SfiI
SfoI SmlI StyI SwaI TaqII TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AgeI AleI AlfI
ApaLI AscI AsiSI BaeI BamHI BciVI BclI BfrBI
BlpI BmtI BsaBI BsaXI BsiWI BspMI BsrFI BsrGI
BssHII BssSI BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI
FspAI HindIII KpnI MfeI MluI MspA1I NaeI NdeI
NgoMIV NheI NruI NsiI PacI PasI PmeI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI TatI Tth111I
XcmI XhoI XmaI ZraI