(Linear) (Six-base) MAPSORT of: KIEE5012.seq Check: 7205 from: 1 to: 3003 KIEE5012.orf With 182 enzymes: SgfI * December 5, 2014 12:57 .. AclI AA'CG_TT Cuts at: 0 1374 3003 Size: 1374 1629 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 605 770 1111 1698 1719 1743 1767 1815 Size: 605 165 341 587 21 24 24 48 Cuts at: 1815 1911 1935 2044 2079 2127 2271 2415 2463 Size: 96 24 109 35 48 144 144 48 Cuts at: 2463 2511 3003 Size: 48 492 Fragments arranged by size: 605 587 492 341 165 144 144 109 96 48 48 48 48 35 24 24 24 21 AflII C'TTAA_G Cuts at: 0 1974 3003 Size: 1974 1029 AflIII A'CryG_T Cuts at: 0 513 826 1152 3003 Size: 513 313 326 1851 Fragments arranged by size: 1851 513 326 313 AhdI GACnn_n'nnGTC Cuts at: 0 315 3003 Size: 315 2688 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 839 871 1681 1713 3003 Size: 839 32 810 32 1290 Fragments arranged by size: 1290 839 810 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 292 1197 3003 Size: 292 905 1806 Fragments arranged by size: 1806 905 292 ApaI G_GGCC'C Cuts at: 0 2440 2978 3003 Size: 2440 538 25 Fragments arranged by size: 2440 538 25 ApoI r'AATT_y Cuts at: 0 598 903 1215 1339 1455 2658 2819 3003 Size: 598 305 312 124 116 1203 161 184 Fragments arranged by size: 1203 598 312 305 184 161 124 116 AseI AT'TA_AT Cuts at: 0 377 776 3003 Size: 377 399 2227 Fragments arranged by size: 2227 399 377 AvaI C'yCGr_G Cuts at: 0 1939 2083 2393 3003 Size: 1939 144 310 610 Fragments arranged by size: 1939 610 310 144 AvrII C'CTAG_G Cuts at: 0 1395 1744 1840 3003 Size: 1395 349 96 1163 Fragments arranged by size: 1395 1163 349 96 BanI G'GyrC_C Cuts at: 0 1655 1787 1811 1835 2315 2411 2459 3003 Size: 1655 132 24 24 480 96 48 544 Fragments arranged by size: 1655 544 480 132 96 48 24 24 BanII G_rGCy'C Cuts at: 0 212 2440 2488 2623 2978 3003 Size: 212 2228 48 135 355 25 Fragments arranged by size: 2228 355 212 135 48 25 BbeI G_GCGC'C Cuts at: 0 1791 1815 2319 3003 Size: 1791 24 504 684 Fragments arranged by size: 1791 684 504 24 BbsI GAAGACnn'nnnn_ Cuts at: 0 247 1673 1760 2432 2456 2552 3003 Size: 247 1426 87 672 24 96 451 Fragments arranged by size: 1426 672 451 247 96 87 24 BbvCI CC'TCA_GC Cuts at: 0 1277 3003 Size: 1277 1726 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2176 2210 3003 Size: 2176 34 793 Fragments arranged by size: 2176 793 34 BglI GCCn_nnn'nGGC Cuts at: 0 1653 2973 3003 Size: 1653 1320 30 Fragments arranged by size: 1653 1320 30 BglII A'GATC_T Cuts at: 0 745 2952 3003 Size: 745 2207 51 Fragments arranged by size: 2207 745 51 Bme1580I G_kGCm'C Cuts at: 0 2440 2978 3003 Size: 2440 538 25 Fragments arranged by size: 2440 538 25 BmgBI CAC'GTC Cuts at: 0 163 3003 Size: 163 2840 BmrI ACTGGGnnnn_n' Cuts at: 0 31 3003 Size: 31 2972 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2195 2243 3003 Size: 2195 48 760 Fragments arranged by size: 2195 760 48 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1646 1875 1899 1912 2043 2213 3003 Size: 1646 229 24 13 131 170 790 Fragments arranged by size: 1646 790 229 170 131 24 13 Bpu10I CC'TnA_GC Cuts at: 0 1277 3003 Size: 1277 1726 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1157 1620 1888 3003 Size: 1157 463 268 1115 Fragments arranged by size: 1157 1115 463 268 BsaI GGTCTCn'nnnn_ Cuts at: 0 806 1916 2214 2334 3003 Size: 806 1110 298 120 669 Fragments arranged by size: 1110 806 669 298 120 BsaAI yAC'GTr Cuts at: 0 514 1504 3003 Size: 514 990 1499 Fragments arranged by size: 1499 990 514 BsaHI Gr'CG_yC Cuts at: 0 282 1788 1812 2316 3003 Size: 282 1506 24 504 687 Fragments arranged by size: 1506 687 504 282 24 BsaWI w'CCGG_w Cuts at: 0 1818 2168 2216 2336 2562 3003 Size: 1818 350 48 120 226 441 Fragments arranged by size: 1818 441 350 226 120 48 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 107 1671 1789 1861 1872 1885 1909 1957 Size: 107 1564 118 72 11 13 24 48 Cuts at: 1957 1981 2029 2053 2125 2149 2581 3003 Size: 24 48 24 72 24 432 422 Fragments arranged by size: 1564 432 422 118 107 72 72 48 48 24 24 24 24 13 11 BseYI C'CCAG_C Cuts at: 0 388 3003 Size: 388 2615 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1267 3003 Size: 1267 1736 BsiEI CG_ry'CG Cuts at: 0 2368 3003 Size: 2368 635 BsiHKAI G_wGCw'C Cuts at: 0 212 2261 2285 2333 3003 Size: 212 2049 24 48 670 Fragments arranged by size: 2049 670 212 48 24 BsmI GAATG_Cn' Cuts at: 0 2124 3003 Size: 2124 879 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2019 3003 Size: 2019 984 Bsp1286I G_dGCh'C Cuts at: 0 212 2261 2285 2333 2440 2488 2623 2978 Size: 212 2049 24 48 107 48 135 355 Cuts at: 2978 3003 Size: 25 Fragments arranged by size: 2049 355 212 135 107 48 48 25 24 BspEI T'CCGG_A Cuts at: 0 1818 2168 2216 2336 2562 3003 Size: 1818 350 48 120 226 441 Fragments arranged by size: 1818 441 350 226 120 48 BspHI T'CATG_A Cuts at: 0 564 3003 Size: 564 2439 BsrBI CCG'CTC Cuts at: 0 1018 3003 Size: 1018 1985 BsrDI GCAATG_nn' Cuts at: 0 1097 3003 Size: 1097 1906 BstAPI GCAn_nnn'nTGC Cuts at: 0 163 3003 Size: 163 2840 BstBI TT'CG_AA Cuts at: 0 792 3003 Size: 792 2211 BstEII G'GTnAC_C Cuts at: 0 1513 3003 Size: 1513 1490 BstYI r'GATC_y Cuts at: 0 289 745 2952 3003 Size: 289 456 2207 51 Fragments arranged by size: 2207 456 289 51 Bsu36I CC'TnA_GG Cuts at: 0 1964 2012 2132 2180 2372 2551 2720 3003 Size: 1964 48 120 48 192 179 169 283 Fragments arranged by size: 1964 283 192 179 169 120 48 48 BtgI C'CryG_G Cuts at: 0 1916 2835 3003 Size: 1916 919 168 Fragments arranged by size: 1916 919 168 DraI TTT'AAA Cuts at: 0 1344 2802 3003 Size: 1344 1458 201 Fragments arranged by size: 1458 1344 201 EaeI y'GGCC_r Cuts at: 0 570 1188 1885 2005 2197 2269 2293 2365 Size: 570 618 697 120 192 72 24 72 Cuts at: 2365 3003 Size: 638 Fragments arranged by size: 697 638 618 570 192 120 72 72 24 EagI C'GGCC_G Cuts at: 0 2365 3003 Size: 2365 638 EarI CTCTTCn'nnn_ Cuts at: 0 270 375 1111 2063 3003 Size: 270 105 736 952 940 Fragments arranged by size: 952 940 736 270 105 EcoICRI GAG'CTC Cuts at: 0 210 3003 Size: 210 2793 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 605 770 1111 1646 1698 1719 1743 1767 Size: 605 165 341 535 52 21 24 24 Cuts at: 1767 1815 1875 1899 1911 1912 1935 2043 2044 Size: 48 60 24 12 1 23 108 1 Cuts at: 2044 2079 2127 2213 2271 2415 2463 2511 3003 Size: 35 48 86 58 144 48 48 492 Fragments arranged by size: 605 535 492 341 165 144 108 86 60 58 52 48 48 48 48 35 24 24 24 23 21 12 1 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 89 1870 3003 Size: 89 1781 1133 Fragments arranged by size: 1781 1133 89 EcoO109I rG'GnC_Cy Cuts at: 0 43 1862 2436 2437 2571 2975 3003 Size: 43 1819 574 1 134 404 28 Fragments arranged by size: 1819 574 404 134 43 28 1 HaeII r_GCGC'y Cuts at: 0 1791 1815 2319 2391 3003 Size: 1791 24 504 72 612 Fragments arranged by size: 1791 612 504 72 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 173 205 301 333 2775 2807 3003 Size: 173 32 96 32 2442 32 196 Fragments arranged by size: 2442 196 173 96 32 32 32 HincII GTy'rAC Cuts at: 0 309 2993 3003 Size: 309 2684 10 Fragments arranged by size: 2684 309 10 HpaI GTT'AAC Cuts at: 0 2993 3003 Size: 2993 10 KasI G'GCGC_C Cuts at: 0 1787 1811 2315 3003 Size: 1787 24 504 688 Fragments arranged by size: 1787 688 504 24 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1698 3003 Size: 1698 1305 MscI TGG'CCA Cuts at: 0 1190 3003 Size: 1190 1813 MslI CAynn'nnrTG Cuts at: 0 401 566 3003 Size: 401 165 2437 Fragments arranged by size: 2437 401 165 NarI GG'CG_CC Cuts at: 0 1788 1812 2316 3003 Size: 1788 24 504 687 Fragments arranged by size: 1788 687 504 24 NcoI C'CATG_G Cuts at: 0 2835 3003 Size: 2835 168 NotI GC'GGCC_GC Cuts at: 0 2365 3003 Size: 2365 638 NspI r_CATG'y Cuts at: 0 1156 3003 Size: 1156 1847 PciI A'CATG_T Cuts at: 0 1152 3003 Size: 1152 1851 PflMI CCAn_nnn'nTGG Cuts at: 0 292 2677 3003 Size: 292 2385 326 Fragments arranged by size: 2385 326 292 PfoI T'CCnGG_A Cuts at: 0 285 3003 Size: 285 2718 PmlI CAC'GTG Cuts at: 0 1504 3003 Size: 1504 1499 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 816 848 3003 Size: 816 32 2155 Fragments arranged by size: 2155 816 32 PpuMI rG'GwC_Cy Cuts at: 0 2571 3003 Size: 2571 432 PspOMI G'GGCC_C Cuts at: 0 2436 2974 3003 Size: 2436 538 29 Fragments arranged by size: 2436 538 29 SacI G_AGCT'C Cuts at: 0 212 3003 Size: 212 2791 SapI GCTCTTCn'nnn_ Cuts at: 0 375 3003 Size: 375 2628 SfcI C'TryA_G Cuts at: 0 5 203 245 2894 3003 Size: 5 198 42 2649 109 Fragments arranged by size: 2649 198 109 42 5 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2973 3003 Size: 2973 30 SfoI GGC'GCC Cuts at: 0 1789 1813 2317 3003 Size: 1789 24 504 686 Fragments arranged by size: 1789 686 504 24 SmlI C'TyrA_G Cuts at: 0 1172 1599 1867 1974 3003 Size: 1172 427 268 107 1029 Fragments arranged by size: 1172 1029 427 268 107 StyI C'CwwG_G Cuts at: 0 1395 1744 1792 1840 2835 3003 Size: 1395 349 48 48 995 168 Fragments arranged by size: 1395 995 349 168 48 48 SwaI ATTT'AAAT Cuts at: 0 1344 3003 Size: 1344 1659 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2429 3003 Size: 2429 574 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1963 1995 3003 Size: 1963 32 1008 Fragments arranged by size: 1963 1008 32 XbaI T'CTAG_A Cuts at: 0 1075 3003 Size: 1075 1928 XmnI GAAnn'nnTTC Cuts at: 0 828 3003 Size: 828 2175 Enzymes that do cut: AclI AcuI AflII AflIII AhdI AloI AlwNI ApaI ApoI AseI AvaI AvrII BanI BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BstAPI BstBI BstEII BstYI Bsu36I BtgI DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HpaI KasI MmeI MscI MslI NarI NcoI NotI NspI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI SacI SapI SfcI SfiI SfoI SmlI StyI SwaI TaqII TstI XbaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AgeI AleI AlfI ApaLI AscI AsiSI BaeI BamHI BciVI BclI BfrBI BlpI BmtI BsaBI BsaXI BsiWI BspMI BsrFI BsrGI BssHII BssSI BstXI BstZ17I BtgZI BtsI ClaI CspCI DraIII DrdI EciI EcoRI EcoRV FalI FseI FspI FspAI HindIII KpnI MfeI MluI MspA1I NaeI NdeI NgoMIV NheI NruI NsiI PacI PasI PmeI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI TatI Tth111I XcmI XhoI XmaI ZraI