Restriction map for
pFN21AE5010
(Linear) (Six-base) MAPSORT of: KIEE5010.seq Check: 5261 from: 1 to: 942
KIEE5010.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 689 942
Size: 689 253
AccI GT'mk_AC
Cuts at: 0 486 942
Size: 486 456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 942
Size: 52 890
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 403 942
Size: 403 539
AlwNI CAG_nnn'CTG
Cuts at: 0 28 112 942
Size: 28 84 830
Fragments arranged by size:
830 84 28
ApoI r'AATT_y
Cuts at: 0 98 942
Size: 98 844
AvrII C'CTAG_G
Cuts at: 0 412 942
Size: 412 530
BbsI GAAGACnn'nnnn_
Cuts at: 0 435 942
Size: 435 507
BbvCI CC'TCA_GC
Cuts at: 0 127 942
Size: 127 815
BciVI GTATCCnnnnn_n'
Cuts at: 0 828 942
Size: 828 114
BfrBI ATG'CAT
Cuts at: 0 290 942
Size: 290 652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 920 942
Size: 552 368 22
Fragments arranged by size:
552 368 22
Bpu10I CC'TnA_GC
Cuts at: 0 127 942
Size: 127 815
BsaI GGTCTCn'nnnn_
Cuts at: 0 764 942
Size: 764 178
BsaAI yAC'GTr
Cuts at: 0 369 942
Size: 369 573
BsiEI CG_ry'CG
Cuts at: 0 363 942
Size: 363 579
BsmI GAATG_Cn'
Cuts at: 0 337 942
Size: 337 605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 689 792 942
Size: 689 103 150
Fragments arranged by size:
689 150 103
BstYI r'GATC_y
Cuts at: 0 268 942
Size: 268 674
BstZ17I GTA'TAC
Cuts at: 0 487 942
Size: 487 455
BtgI C'CryG_G
Cuts at: 0 350 474 942
Size: 350 124 468
Fragments arranged by size:
468 350 124
BtsI GCAGTG_nn'
Cuts at: 0 385 942
Size: 385 557
EaeI y'GGCC_r
Cuts at: 0 371 623 942
Size: 371 252 319
Fragments arranged by size:
371 319 252
EarI CTCTTCn'nnn_
Cuts at: 0 85 94 942
Size: 85 9 848
Fragments arranged by size:
848 85 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 552 920 942
Size: 52 500 368 22
Fragments arranged by size:
500 368 52 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 82 942
Size: 82 860
FspI TGC'GCA
Cuts at: 0 679 942
Size: 679 263
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 500 532 802 834 855 887 942
Size: 500 32 270 32 21 32 55
Fragments arranged by size:
500 270 55 32 32 32 21
HincII GTy'rAC
Cuts at: 0 160 942
Size: 160 782
MscI TGG'CCA
Cuts at: 0 373 625 942
Size: 373 252 317
Fragments arranged by size:
373 317 252
MspA1I CmG'CkG
Cuts at: 0 72 300 739 942
Size: 72 228 439 203
Fragments arranged by size:
439 228 203 72
NcoI C'CATG_G
Cuts at: 0 350 474 942
Size: 350 124 468
Fragments arranged by size:
468 350 124
NdeI CA'TA_TG
Cuts at: 0 651 942
Size: 651 291
NsiI A_TGCA'T
Cuts at: 0 292 942
Size: 292 650
NspI r_CATG'y
Cuts at: 0 342 942
Size: 342 600
PfoI T'CCnGG_A
Cuts at: 0 936 942
Size: 936 6
PstI C_TGCA'G
Cuts at: 0 382 942
Size: 382 560
PvuI CG_AT'CG
Cuts at: 0 363 942
Size: 363 579
PvuII CAG'CTG
Cuts at: 0 72 300 739 942
Size: 72 228 439 203
Fragments arranged by size:
439 228 203 72
SfcI C'TryA_G
Cuts at: 0 378 720 942
Size: 378 342 222
Fragments arranged by size:
378 342 222
StyI C'CwwG_G
Cuts at: 0 350 412 474 942
Size: 350 62 62 468
Fragments arranged by size:
468 350 62 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 820 942
Size: 820 122
TatI w'GTAC_w
Cuts at: 0 176 942
Size: 176 766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 828 860 942
Size: 828 32 82
Fragments arranged by size:
828 82 32
Tth111I GACn'n_nGTC
Cuts at: 0 541 942
Size: 541 401
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApoI AvrII BbsI
BbvCI BciVI BfrBI BpmI Bpu10I BsaI BsaAI BsiEI
BsmI BspMI BstYI BstZ17I BtgI BtsI EaeI EarI
Eco57MI FalI FspI Hin4I HincII MscI MspA1I NcoI
NdeI NsiI NspI PfoI PstI PvuI PvuII SfcI
StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XcmI XhoI XmaI XmnI ZraI