Restriction map for
pFN21AE5008
(Linear) (Six-base) MAPSORT of: KIEE5008.seq Check: 7483 from: 1 to: 1005
KIEE5008.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 747 1005
Size: 747 258
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 526 678 1005
Size: 281 245 152 327
Fragments arranged by size:
327 281 245 152
AfeI AGC'GCT
Cuts at: 0 421 1005
Size: 421 584
AhdI GACnn_n'nnGTC
Cuts at: 0 192 1005
Size: 192 813
AleI CACnn'nnGTG
Cuts at: 0 613 925 1005
Size: 613 312 80
Fragments arranged by size:
613 312 80
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 943 1005
Size: 943 62
AlwNI CAG_nnn'CTG
Cuts at: 0 504 537 871 1001 1005
Size: 504 33 334 130 4
Fragments arranged by size:
504 334 130 33 4
ApoI r'AATT_y
Cuts at: 0 112 1005
Size: 112 893
BanII G_rGCy'C
Cuts at: 0 631 1005
Size: 631 374
BbsI GAAGACnn'nnnn_
Cuts at: 0 413 1005
Size: 413 592
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 427 461 1005
Size: 427 34 544
Fragments arranged by size:
544 427 34
BglII A'GATC_T
Cuts at: 0 57 275 1005
Size: 57 218 730
Fragments arranged by size:
730 218 57
Bme1580I G_kGCm'C
Cuts at: 0 798 1005
Size: 798 207
BmgBI CAC'GTC
Cuts at: 0 700 1005
Size: 700 305
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 820 1005
Size: 370 450 185
Fragments arranged by size:
450 370 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 654 1005
Size: 654 351
BsaI GGTCTCn'nnnn_
Cuts at: 0 179 767 1005
Size: 179 588 238
Fragments arranged by size:
588 238 179
BsaWI w'CCGG_w
Cuts at: 0 10 1005
Size: 10 995
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 1005
Size: 46 959
BseYI C'CCAG_C
Cuts at: 0 580 664 864 1005
Size: 580 84 200 141
Fragments arranged by size:
580 200 141 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 565 637 1005
Size: 271 294 72 368
Fragments arranged by size:
368 294 271 72
BsmBI CGTCTCn'nnnn_
Cuts at: 0 145 900 1005
Size: 145 755 105
Fragments arranged by size:
755 145 105
Bsp1286I G_dGCh'C
Cuts at: 0 631 798 1005
Size: 631 167 207
Fragments arranged by size:
631 207 167
BspEI T'CCGG_A
Cuts at: 0 10 1005
Size: 10 995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 747 1005
Size: 747 258
BsrDI GCAATG_nn'
Cuts at: 0 647 1005
Size: 647 358
BsrFI r'CCGG_y
Cuts at: 0 269 1005
Size: 269 736
BssHII G'CGCG_C
Cuts at: 0 384 1005
Size: 384 621
BssSI C'ACGA_G
Cuts at: 0 829 954 1005
Size: 829 125 51
Fragments arranged by size:
829 125 51
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 1005
Size: 588 417
BstYI r'GATC_y
Cuts at: 0 57 275 1005
Size: 57 218 730
Fragments arranged by size:
730 218 57
BtgI C'CryG_G
Cuts at: 0 623 1005
Size: 623 382
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 79 1005
Size: 79 926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 630 665 1005
Size: 630 35 340
Fragments arranged by size:
630 340 35
EaeI y'GGCC_r
Cuts at: 0 539 910 1005
Size: 539 371 95
Fragments arranged by size:
539 371 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 891 1005
Size: 891 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 370 526 678 820 1005
Size: 281 89 156 152 142 185
Fragments arranged by size:
281 185 156 152 142 89
EcoO109I rG'GnC_Cy
Cuts at: 0 569 661 725 1005
Size: 569 92 64 280
Fragments arranged by size:
569 280 92 64
EcoRI G'AATT_C
Cuts at: 0 112 1005
Size: 112 893
EcoRV GAT'ATC
Cuts at: 0 752 1005
Size: 752 253
HaeII r_GCGC'y
Cuts at: 0 423 1005
Size: 423 582
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 178 210 384 416 1005
Size: 178 32 174 32 589
Fragments arranged by size:
589 178 174 32 32
MfeI C'AATT_G
Cuts at: 0 325 1005
Size: 325 680
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1005
Size: 929 76
MslI CAynn'nnrTG
Cuts at: 0 394 613 925 1005
Size: 394 219 312 80
Fragments arranged by size:
394 312 219 80
MspA1I CmG'CkG
Cuts at: 0 159 613 965 1005
Size: 159 454 352 40
Fragments arranged by size:
454 352 159 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 871 1005
Size: 871 134
PfoI T'CCnGG_A
Cuts at: 0 116 452 485 1005
Size: 116 336 33 520
Fragments arranged by size:
520 336 116 33
PpuMI rG'GwC_Cy
Cuts at: 0 569 661 725 1005
Size: 569 92 64 280
Fragments arranged by size:
569 280 92 64
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 817 849 1005
Size: 817 32 156
Fragments arranged by size:
817 156 32
PvuII CAG'CTG
Cuts at: 0 159 613 1005
Size: 159 454 392
Fragments arranged by size:
454 392 159
SanDI GG'GwC_CC
Cuts at: 0 725 1005
Size: 725 280
SexAI A'CCwGG_T
Cuts at: 0 985 1005
Size: 985 20
SmlI C'TyrA_G
Cuts at: 0 669 1005
Size: 669 336
StyI C'CwwG_G
Cuts at: 0 284 1005
Size: 284 721
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 925 1005
Size: 925 80
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 930 1005
Size: 930 75
XmnI GAAnn'nnTTC
Cuts at: 0 494 1005
Size: 494 511
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI AlfI AlwNI ApoI
BanII BbsI BcgI BglII Bme1580I BmgBI BpmI BpuEI
BsaI BsaWI BseRI BseYI BsgI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrFI BssHII BssSI BstXI BstYI BtgI
BtgZI CspCI EaeI EciI Eco57MI EcoO109I EcoRI EcoRV
HaeII Hin4I MfeI MmeI MslI MspA1I PflMI PfoI
PpuMI PsrI PvuII SanDI SexAI SmlI StyI TaqII
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglI
BlpI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaXI BsiEI BsiHKAI BsiWI BsmI BspHI BsrBI BsrGI
BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI DraI
DraIII DrdI EagI EarI EcoICRI EcoNI FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmaI ZraI