Restriction map for
pFN21AE5005
(Linear) (Six-base) MAPSORT of: KIEE5005.seq Check: 1919 from: 1 to: 1401
KIEE5005.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AatII G_ACGT'C
Cuts at: 0 1001 1401
Size: 1001 400
Acc65I G'GTAC_C
Cuts at: 0 179 1401
Size: 179 1222
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 691 1243 1401
Size: 505 186 552 158
Fragments arranged by size:
552 505 186 158
AflIII A'CryG_T
Cuts at: 0 623 705 1401
Size: 623 82 696
Fragments arranged by size:
696 623 82
AgeI A'CCGG_T
Cuts at: 0 209 1401
Size: 209 1192
AleI CACnn'nnGTG
Cuts at: 0 931 1401
Size: 931 470
ApaI G_GGCC'C
Cuts at: 0 1106 1401
Size: 1106 295
ApoI r'AATT_y
Cuts at: 0 807 1291 1401
Size: 807 484 110
Fragments arranged by size:
807 484 110
AvaI C'yCGr_G
Cuts at: 0 366 1401
Size: 366 1035
AvrII C'CTAG_G
Cuts at: 0 1098 1401
Size: 1098 303
BanI G'GyrC_C
Cuts at: 0 179 1401
Size: 179 1222
BanII G_rGCy'C
Cuts at: 0 229 644 1106 1401
Size: 229 415 462 295
Fragments arranged by size:
462 415 295 229
BfrBI ATG'CAT
Cuts at: 0 3 1401
Size: 3 1398
BglI GCCn_nnn'nGGC
Cuts at: 0 443 1401
Size: 443 958
BglII A'GATC_T
Cuts at: 0 136 1401
Size: 136 1265
Bme1580I G_kGCm'C
Cuts at: 0 987 1106 1401
Size: 987 119 295
Fragments arranged by size:
987 295 119
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1200 1401
Size: 1200 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1023 1270 1401
Size: 1023 247 131
Fragments arranged by size:
1023 247 131
Bpu10I CC'TnA_GC
Cuts at: 0 46 1401
Size: 46 1355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1401
Size: 991 410
BsaAI yAC'GTr
Cuts at: 0 1257 1401
Size: 1257 144
BsaHI Gr'CG_yC
Cuts at: 0 998 1401
Size: 998 403
BsaWI w'CCGG_w
Cuts at: 0 209 302 1401
Size: 209 93 1099
Fragments arranged by size:
1099 209 93
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 81 189 354 865 1293 1378 1401
Size: 78 3 108 165 511 428 85 23
Fragments arranged by size:
511 428 165 108 85 78 23 3
BseYI C'CCAG_C
Cuts at: 0 975 980 1304 1401
Size: 975 5 324 97
Fragments arranged by size:
975 324 97 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1279 1318 1401
Size: 1279 39 83
Fragments arranged by size:
1279 83 39
BsiEI CG_ry'CG
Cuts at: 0 1194 1401
Size: 1194 207
BsiHKAI G_wGCw'C
Cuts at: 0 545 644 839 920 1076 1256 1401
Size: 545 99 195 81 156 180 145
Fragments arranged by size:
545 195 180 156 145 99 81
BsmI GAATG_Cn'
Cuts at: 0 1016 1401
Size: 1016 385
Bsp1286I G_dGCh'C
Cuts at: 0 229 545 644 839 920 987 1076 1106
Size: 229 316 99 195 81 67 89 30
Cuts at: 1106 1256 1401
Size: 150 145
Fragments arranged by size:
316 229 195 150 145 99 89 81
67 30
BsrBI CCG'CTC
Cuts at: 0 23 222 1368 1401
Size: 23 199 1146 33
Fragments arranged by size:
1146 199 33 23
BsrFI r'CCGG_y
Cuts at: 0 209 1193 1401
Size: 209 984 208
Fragments arranged by size:
984 209 208
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1013 1401
Size: 1013 388
BstEII G'GTnAC_C
Cuts at: 0 424 1401
Size: 424 977
BstXI CCAn_nnnn'nTGG
Cuts at: 0 933 1401
Size: 933 468
BstYI r'GATC_y
Cuts at: 0 136 1094 1401
Size: 136 958 307
Fragments arranged by size:
958 307 136
Bsu36I CC'TnA_GG
Cuts at: 0 959 1361 1401
Size: 959 402 40
Fragments arranged by size:
959 402 40
BtgI C'CryG_G
Cuts at: 0 615 1401
Size: 615 786
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 615 1401
Size: 615 786
BtsI GCAGTG_nn'
Cuts at: 0 324 903 1401
Size: 324 579 498
Fragments arranged by size:
579 498 324
DraIII CAC_nnn'GTG
Cuts at: 0 432 1080 1401
Size: 432 648 321
Fragments arranged by size:
648 432 321
EaeI y'GGCC_r
Cuts at: 0 935 1191 1319 1401
Size: 935 256 128 82
Fragments arranged by size:
935 256 128 82
EagI C'GGCC_G
Cuts at: 0 1191 1401
Size: 1191 210
EarI CTCTTCn'nnn_
Cuts at: 0 55 212 1054 1219 1401
Size: 55 157 842 165 182
Fragments arranged by size:
842 182 165 157 55
EcoICRI GAG'CTC
Cuts at: 0 642 1401
Size: 642 759
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 691 1023 1243 1270 1401
Size: 505 186 332 220 27 131
Fragments arranged by size:
505 332 220 186 131 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 171 1401
Size: 171 1230
EcoO109I rG'GnC_Cy
Cuts at: 0 71 1102 1161 1326 1401
Size: 71 1031 59 165 75
Fragments arranged by size:
1031 165 75 71 59
EcoRI G'AATT_C
Cuts at: 0 807 1401
Size: 807 594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 675 1401
Size: 675 726
HaeII r_GCGC'y
Cuts at: 0 192 362 1401
Size: 192 170 1039
Fragments arranged by size:
1039 192 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 841 873 1354 1386 1401
Size: 841 32 481 32 15
Fragments arranged by size:
841 481 32 32 15
KpnI G_GTAC'C
Cuts at: 0 183 1401
Size: 183 1218
MscI TGG'CCA
Cuts at: 0 937 1401
Size: 937 464
MslI CAynn'nnrTG
Cuts at: 0 210 880 931 1401
Size: 210 670 51 470
Fragments arranged by size:
670 470 210 51
MspA1I CmG'CkG
Cuts at: 0 765 1263 1401
Size: 765 498 138
Fragments arranged by size:
765 498 138
NsiI A_TGCA'T
Cuts at: 0 5 1401
Size: 5 1396
NspI r_CATG'y
Cuts at: 0 319 627 709 1236 1401
Size: 319 308 82 527 165
Fragments arranged by size:
527 319 308 165 82
PciI A'CATG_T
Cuts at: 0 623 705 1401
Size: 623 82 696
Fragments arranged by size:
696 623 82
PflMI CCAn_nnn'nTGG
Cuts at: 0 235 1401
Size: 235 1166
PmlI CAC'GTG
Cuts at: 0 1257 1401
Size: 1257 144
PpuMI rG'GwC_Cy
Cuts at: 0 71 1401
Size: 71 1330
PspOMI G'GGCC_C
Cuts at: 0 1102 1401
Size: 1102 299
PvuII CAG'CTG
Cuts at: 0 765 1263 1401
Size: 765 498 138
Fragments arranged by size:
765 498 138
SacI G_AGCT'C
Cuts at: 0 644 1401
Size: 644 757
SapI GCTCTTCn'nnn_
Cuts at: 0 212 1401
Size: 212 1189
ScaI AGT'ACT
Cuts at: 0 1030 1401
Size: 1030 371
SexAI A'CCwGG_T
Cuts at: 0 343 428 1076 1401
Size: 343 85 648 325
Fragments arranged by size:
648 343 325 85
SfcI C'TryA_G
Cuts at: 0 75 1401
Size: 75 1326
SmlI C'TyrA_G
Cuts at: 0 366 970 1401
Size: 366 604 431
Fragments arranged by size:
604 431 366
SphI G_CATG'C
Cuts at: 0 319 1401
Size: 319 1082
SspI AAT'ATT
Cuts at: 0 1147 1401
Size: 1147 254
StyI C'CwwG_G
Cuts at: 0 1098 1401
Size: 1098 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 131 1401
Size: 131 1270
TatI w'GTAC_w
Cuts at: 0 1028 1401
Size: 1028 373
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1106 1138 1401
Size: 1106 32 263
Fragments arranged by size:
1106 263 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 879 1401
Size: 879 522
XhoI C'TCGA_G
Cuts at: 0 366 1401
Size: 366 1035
XmnI GAAnn'nnTTC
Cuts at: 0 682 1147 1401
Size: 682 465 254
Fragments arranged by size:
682 465 254
ZraI GAC'GTC
Cuts at: 0 999 1401
Size: 999 402
Enzymes that do cut:
AatII Acc65I AcuI AflIII AgeI AleI ApaI ApoI
AvaI AvrII BanI BanII BfrBI BglI BglII Bme1580I
BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrFI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I KpnI MscI MslI MspA1I
NsiI NspI PciI PflMI PmlI PpuMI PspOMI PvuII
SacI SapI ScaI SexAI SfcI SmlI SphI SspI
StyI TaqII TatI TstI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AhdI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI BaeI BamHI BbeI
BbsI BbvCI BcgI BciVI BclI BlpI BmgBI BmrI
BmtI BsaI BsaBI BsaXI BsiWI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI BstBI BstZ17I ClaI
CspCI DraI DrdI EciI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PfoI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI Tth111I XbaI XmaI