Restriction map for
pFN21AE5004
(Linear) (Six-base) MAPSORT of: KIEE5004.seq Check: 9864 from: 1 to: 2742
KIEE5004.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1930 2742
Size: 1930 812
AatII G_ACGT'C
Cuts at: 0 1150 2742
Size: 1150 1592
AccI GT'mk_AC
Cuts at: 0 325 1970 2742
Size: 325 1645 772
Fragments arranged by size:
1645 772 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1121 1361 2296 2742
Size: 1121 240 935 446
Fragments arranged by size:
1121 935 446 240
AfeI AGC'GCT
Cuts at: 0 1453 2742
Size: 1453 1289
AflIII A'CryG_T
Cuts at: 0 723 2742
Size: 723 2019
AhdI GACnn_n'nnGTC
Cuts at: 0 2624 2742
Size: 2624 118
AlwNI CAG_nnn'CTG
Cuts at: 0 1945 2326 2742
Size: 1945 381 416
Fragments arranged by size:
1945 416 381
ApaI G_GGCC'C
Cuts at: 0 57 1316 1422 2742
Size: 57 1259 106 1320
Fragments arranged by size:
1320 1259 106 57
ApoI r'AATT_y
Cuts at: 0 520 742 1807 2285 2742
Size: 520 222 1065 478 457
Fragments arranged by size:
1065 520 478 457 222
AseI AT'TA_AT
Cuts at: 0 2147 2306 2742
Size: 2147 159 436
Fragments arranged by size:
2147 436 159
AvaI C'yCGr_G
Cuts at: 0 2196 2742
Size: 2196 546
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 569 602 2742
Size: 569 33 2140
Fragments arranged by size:
2140 569 33
BamHI G'GATC_C
Cuts at: 0 392 1596 2742
Size: 392 1204 1146
Fragments arranged by size:
1204 1146 392
BanI G'GyrC_C
Cuts at: 0 1285 2742
Size: 1285 1457
BanII G_rGCy'C
Cuts at: 0 57 477 1316 1422 2031 2742
Size: 57 420 839 106 609 711
Fragments arranged by size:
839 711 609 420 106 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 262 1247 2742
Size: 262 985 1495
Fragments arranged by size:
1495 985 262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1500 1534 2742
Size: 1500 34 1208
Fragments arranged by size:
1500 1208 34
BglII A'GATC_T
Cuts at: 0 2514 2742
Size: 2514 228
Bme1580I G_kGCm'C
Cuts at: 0 57 1316 1422 2742
Size: 57 1259 106 1320
Fragments arranged by size:
1320 1259 106 57
BmgBI CAC'GTC
Cuts at: 0 1739 2742
Size: 1739 1003
BmrI ACTGGGnnnn_n'
Cuts at: 0 1595 2742
Size: 1595 1147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 1471 2742
Size: 317 1154 1271
Fragments arranged by size:
1271 1154 317
Bpu10I CC'TnA_GC
Cuts at: 0 1676 2742
Size: 1676 1066
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1310 1486 1959 2742
Size: 307 1003 176 473 783
Fragments arranged by size:
1003 783 473 307 176
BsaI GGTCTCn'nnnn_
Cuts at: 0 270 283 2742
Size: 270 13 2459
Fragments arranged by size:
2459 270 13
BsaHI Gr'CG_yC
Cuts at: 0 1147 2742
Size: 1147 1595
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1749 1779 2628 2658 2742
Size: 1749 30 849 30 84
Fragments arranged by size:
1749 849 84 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 320 1351 2051 2742
Size: 320 1031 700 691
Fragments arranged by size:
1031 700 691 320
BseYI C'CCAG_C
Cuts at: 0 1017 1813 2069 2742
Size: 1017 796 256 673
Fragments arranged by size:
1017 796 673 256
BsiEI CG_ry'CG
Cuts at: 0 1171 2742
Size: 1171 1571
BsiHKAI G_wGCw'C
Cuts at: 0 2085 2742
Size: 2085 657
BsmI GAATG_Cn'
Cuts at: 0 2684 2742
Size: 2684 58
Bsp1286I G_dGCh'C
Cuts at: 0 57 477 1316 1422 2031 2085 2742
Size: 57 420 839 106 609 54 657
Fragments arranged by size:
839 657 609 420 106 57 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 556 1134 1930 2742
Size: 556 578 796 812
Fragments arranged by size:
812 796 578 556
BsrDI GCAATG_nn'
Cuts at: 0 413 892 1670 2522 2573 2742
Size: 413 479 778 852 51 169
Fragments arranged by size:
852 778 479 413 169 51
BsrGI T'GTAC_A
Cuts at: 0 748 1062 2087 2742
Size: 748 314 1025 655
Fragments arranged by size:
1025 748 655 314
BssSI C'ACGA_G
Cuts at: 0 2579 2742
Size: 2579 163
BstBI TT'CG_AA
Cuts at: 0 2370 2742
Size: 2370 372
BstEII G'GTnAC_C
Cuts at: 0 168 958 2742
Size: 168 790 1784
Fragments arranged by size:
1784 790 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1821 2077 2742
Size: 1821 256 665
Fragments arranged by size:
1821 665 256
BstYI r'GATC_y
Cuts at: 0 392 1210 1596 2514 2742
Size: 392 818 386 918 228
Fragments arranged by size:
918 818 392 386 228
BtgI C'CryG_G
Cuts at: 0 1532 2343 2742
Size: 1532 811 399
Fragments arranged by size:
1532 811 399
BtsI GCAGTG_nn'
Cuts at: 0 1101 2742
Size: 1101 1641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1263 1298 2742
Size: 1263 35 1444
Fragments arranged by size:
1444 1263 35
DraI TTT'AAA
Cuts at: 0 2696 2742
Size: 2696 46
DrdI GACnn_nn'nnGTC
Cuts at: 0 1737 2742
Size: 1737 1005
EaeI y'GGCC_r
Cuts at: 0 2259 2742
Size: 2259 483
EarI CTCTTCn'nnn_
Cuts at: 0 2254 2423 2742
Size: 2254 169 319
Fragments arranged by size:
2254 319 169
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2296 2600 2742
Size: 2296 304 142
Fragments arranged by size:
2296 304 142
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 1121 1361 1471 2296 2742
Size: 317 804 240 110 825 446
Fragments arranged by size:
825 804 446 317 240 110
EcoO109I rG'GnC_Cy
Cuts at: 0 53 54 1022 1312 1313 1418 1419 2742
Size: 53 1 968 290 1 105 1 1323
Fragments arranged by size:
1323 968 290 105 53 1 1 1
EcoRI G'AATT_C
Cuts at: 0 742 2742
Size: 742 2000
HaeII r_GCGC'y
Cuts at: 0 1455 2742
Size: 1455 1287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 1327 1359 1469 1501 2542 2574
Size: 1006 32 289 32 110 32 1041 32
Cuts at: 2574 2610 2623 2642 2655 2742
Size: 36 13 19 13 87
Fragments arranged by size:
1041 1006 289 110 87 36 32 32
32 32 19 13 13
HincII GTy'rAC
Cuts at: 0 2043 2742
Size: 2043 699
HpaI GTT'AAC
Cuts at: 0 2043 2742
Size: 2043 699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1225 2594 2742
Size: 1225 1369 148
Fragments arranged by size:
1369 1225 148
MscI TGG'CCA
Cuts at: 0 2261 2742
Size: 2261 481
MslI CAynn'nnrTG
Cuts at: 0 859 1645 2742
Size: 859 786 1097
Fragments arranged by size:
1097 859 786
MspA1I CmG'CkG
Cuts at: 0 78 611 1160 2730 2742
Size: 78 533 549 1570 12
Fragments arranged by size:
1570 549 533 78 12
NcoI C'CATG_G
Cuts at: 0 2343 2742
Size: 2343 399
NdeI CA'TA_TG
Cuts at: 0 1371 2742
Size: 1371 1371
NspI r_CATG'y
Cuts at: 0 727 2742
Size: 727 2015
PciI A'CATG_T
Cuts at: 0 723 2742
Size: 723 2019
PflMI CCAn_nnn'nTGG
Cuts at: 0 1207 2742
Size: 1207 1535
PpuMI rG'GwC_Cy
Cuts at: 0 1022 2742
Size: 1022 1720
PspOMI G'GGCC_C
Cuts at: 0 53 1312 1418 2742
Size: 53 1259 106 1324
Fragments arranged by size:
1324 1259 106 53
PstI C_TGCA'G
Cuts at: 0 902 1007 1131 1159 1323 2729 2742
Size: 902 105 124 28 164 1406 13
Fragments arranged by size:
1406 902 164 124 105 28 13
PvuI CG_AT'CG
Cuts at: 0 1171 2742
Size: 1171 1571
PvuII CAG'CTG
Cuts at: 0 78 611 2730 2742
Size: 78 533 2119 12
Fragments arranged by size:
2119 533 78 12
SfcI C'TryA_G
Cuts at: 0 200 734 898 1003 1127 1155 1319 2725
Size: 200 534 164 105 124 28 164 1406
Cuts at: 2725 2742
Size: 17
Fragments arranged by size:
1406 534 200 164 164 124 105 28
17
SmlI C'TyrA_G
Cuts at: 0 286 1325 1465 1974 2742
Size: 286 1039 140 509 768
Fragments arranged by size:
1039 768 509 286 140
SspI AAT'ATT
Cuts at: 0 1839 2658 2742
Size: 1839 819 84
Fragments arranged by size:
1839 819 84
StyI C'CwwG_G
Cuts at: 0 1845 1916 2343 2742
Size: 1845 71 427 399
Fragments arranged by size:
1845 427 399 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1247 2742
Size: 1247 1495
TatI w'GTAC_w
Cuts at: 0 503 748 1062 2087 2377 2742
Size: 503 245 314 1025 290 365
Fragments arranged by size:
1025 503 365 314 290 245
Tth111I GACn'n_nGTC
Cuts at: 0 1966 2742
Size: 1966 776
XbaI T'CTAG_A
Cuts at: 0 45 2742
Size: 45 2697
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1504 2742
Size: 1504 1238
XmnI GAAnn'nnTTC
Cuts at: 0 2417 2742
Size: 2417 325
ZraI GAC'GTC
Cuts at: 0 1148 2742
Size: 1148 1594
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AlwNI
ApaI ApoI AseI AvaI BaeI BamHI BanI BanII
BbsI BcgI BglII Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstYI BtgI BtsI CspCI DraI DrdI EaeI
EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII
HpaI MmeI MscI MslI MspA1I NcoI NdeI NspI
PciI PflMI PpuMI PspOMI PstI PvuI PvuII SfcI
SmlI SspI StyI TaqII TatI Tth111I XbaI XcmI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AleI AlfI AloI ApaLI
AscI AsiSI AvrII BbeI BbvCI BciVI BclI BfrBI
BglI BlpI BmtI BplI BsaAI BsaBI BsaWI BsgI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BstAPI
BstZ17I Bsu36I BtgZI ClaI DraIII EagI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HindIII KasI KpnI
MfeI MluI NaeI NarI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI
XhoI XmaI