Restriction map for
pFN21AE5002
(Linear) (Six-base) MAPSORT of: KIEE5002.seq Check: 565 from: 1 to: 1908
KIEE5002.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AccI GT'mk_AC
Cuts at: 0 564 1908
Size: 564 1344
Acc65I G'GTAC_C
Cuts at: 0 926 1908
Size: 926 982
AclI AA'CG_TT
Cuts at: 0 275 1908
Size: 275 1633
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 880 890 1489 1858 1908
Size: 642 238 10 599 369 50
Fragments arranged by size:
642 599 369 238 50 10
AflII C'TTAA_G
Cuts at: 0 549 1908
Size: 549 1359
AflIII A'CryG_T
Cuts at: 0 983 1242 1849 1908
Size: 983 259 607 59
Fragments arranged by size:
983 607 259 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1618 1908
Size: 1618 290
AlwNI CAG_nnn'CTG
Cuts at: 0 1396 1488 1693 1908
Size: 1396 92 205 215
Fragments arranged by size:
1396 215 205 92
ApoI r'AATT_y
Cuts at: 0 573 626 811 1225 1294 1349 1717 1908
Size: 573 53 185 414 69 55 368 191
Fragments arranged by size:
573 414 368 191 185 69 55 53
BanI G'GyrC_C
Cuts at: 0 39 926 1908
Size: 39 887 982
Fragments arranged by size:
982 887 39
BbeI G_GCGC'C
Cuts at: 0 43 1908
Size: 43 1865
BbsI GAAGACnn'nnnn_
Cuts at: 0 144 244 1633 1908
Size: 144 100 1389 275
Fragments arranged by size:
1389 275 144 100
BciVI GTATCCnnnnn_n'
Cuts at: 0 218 1908
Size: 218 1690
BfrBI ATG'CAT
Cuts at: 0 590 1908
Size: 590 1318
BmrI ACTGGGnnnn_n'
Cuts at: 0 252 844 911 1908
Size: 252 592 67 997
Fragments arranged by size:
997 592 252 67
BsaBI GATnn'nnATC
Cuts at: 0 1195 1908
Size: 1195 713
BsaHI Gr'CG_yC
Cuts at: 0 40 62 344 1908
Size: 40 22 282 1564
Fragments arranged by size:
1564 282 40 22
BsaWI w'CCGG_w
Cuts at: 0 9 1908
Size: 9 1899
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 49 79 1908
Size: 49 30 1829
Fragments arranged by size:
1829 49 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 100 1030 1908
Size: 57 43 930 878
Fragments arranged by size:
930 878 57 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 69 91 1908
Size: 69 22 1817
Fragments arranged by size:
1817 69 22
BspHI T'CATG_A
Cuts at: 0 1007 1908
Size: 1007 901
BsrFI r'CCGG_y
Cuts at: 0 36 1908
Size: 36 1872
BssSI C'ACGA_G
Cuts at: 0 1045 1908
Size: 1045 863
BstBI TT'CG_AA
Cuts at: 0 155 1908
Size: 155 1753
BstEII G'GTnAC_C
Cuts at: 0 615 1908
Size: 615 1293
BstYI r'GATC_y
Cuts at: 0 1190 1908
Size: 1190 718
Bsu36I CC'TnA_GG
Cuts at: 0 136 1908
Size: 136 1772
BtgI C'CryG_G
Cuts at: 0 1823 1908
Size: 1823 85
BtsI GCAGTG_nn'
Cuts at: 0 1418 1460 1908
Size: 1418 42 448
Fragments arranged by size:
1418 448 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 350 385 1908
Size: 350 35 1523
Fragments arranged by size:
1523 350 35
EaeI y'GGCC_r
Cuts at: 0 841 1908
Size: 841 1067
EarI CTCTTCn'nnn_
Cuts at: 0 152 158 1908
Size: 152 6 1750
Fragments arranged by size:
1750 152 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 880 890 1489 1858 1908
Size: 642 238 10 599 369 50
Fragments arranged by size:
642 599 369 238 50 10
EcoO109I rG'GnC_Cy
Cuts at: 0 140 900 1908
Size: 140 760 1008
Fragments arranged by size:
1008 760 140
EcoRV GAT'ATC
Cuts at: 0 1377 1908
Size: 1377 531
HaeII r_GCGC'y
Cuts at: 0 43 1908
Size: 43 1865
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1366 1398 1908
Size: 1366 32 510
Fragments arranged by size:
1366 510 32
HincII GTy'rAC
Cuts at: 0 797 1908
Size: 797 1111
HindIII A'AGCT_T
Cuts at: 0 1095 1908
Size: 1095 813
KasI G'GCGC_C
Cuts at: 0 39 1908
Size: 39 1869
KpnI G_GTAC'C
Cuts at: 0 930 1908
Size: 930 978
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 1908
Size: 258 1650
MscI TGG'CCA
Cuts at: 0 843 1908
Size: 843 1065
MslI CAynn'nnrTG
Cuts at: 0 1600 1908
Size: 1600 308
MspA1I CmG'CkG
Cuts at: 0 1485 1908
Size: 1485 423
NaeI GCC'GGC
Cuts at: 0 38 1908
Size: 38 1870
NarI GG'CG_CC
Cuts at: 0 40 1908
Size: 40 1868
NcoI C'CATG_G
Cuts at: 0 1823 1908
Size: 1823 85
NgoMIV G'CCGG_C
Cuts at: 0 36 1908
Size: 36 1872
NsiI A_TGCA'T
Cuts at: 0 592 1908
Size: 592 1316
NspI r_CATG'y
Cuts at: 0 1246 1599 1853 1908
Size: 1246 353 254 55
Fragments arranged by size:
1246 353 254 55
PasI CC'CwG_GG
Cuts at: 0 21 1908
Size: 21 1887
PciI A'CATG_T
Cuts at: 0 1242 1849 1908
Size: 1242 607 59
Fragments arranged by size:
1242 607 59
PfoI T'CCnGG_A
Cuts at: 0 446 1908
Size: 446 1462
PpuMI rG'GwC_Cy
Cuts at: 0 900 1908
Size: 900 1008
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 961 993 1908
Size: 961 32 915
Fragments arranged by size:
961 915 32
PstI C_TGCA'G
Cuts at: 0 176 1455 1581 1908
Size: 176 1279 126 327
Fragments arranged by size:
1279 327 176 126
PvuII CAG'CTG
Cuts at: 0 1485 1908
Size: 1485 423
SanDI GG'GwC_CC
Cuts at: 0 900 1908
Size: 900 1008
SfcI C'TryA_G
Cuts at: 0 172 415 523 1451 1577 1908
Size: 172 243 108 928 126 331
Fragments arranged by size:
928 331 243 172 126 108
SfoI GGC'GCC
Cuts at: 0 41 1908
Size: 41 1867
SmlI C'TyrA_G
Cuts at: 0 549 1908
Size: 549 1359
StyI C'CwwG_G
Cuts at: 0 92 1673 1823 1908
Size: 92 1581 150 85
Fragments arranged by size:
1581 150 92 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 130 1158 1284 1299 1908
Size: 130 1028 126 15 609
Fragments arranged by size:
1028 609 130 126 15
TatI w'GTAC_w
Cuts at: 0 318 1109 1125 1908
Size: 318 791 16 783
Fragments arranged by size:
791 783 318 16
XmnI GAAnn'nnTTC
Cuts at: 0 814 1295 1775 1908
Size: 814 481 480 133
Fragments arranged by size:
814 481 480 133
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AlwNI
ApoI BanI BbeI BbsI BciVI BfrBI BmrI BsaBI
BsaHI BsaWI BsaXI BseRI BsmBI BspHI BsrFI BssSI
BstBI BstEII BstYI Bsu36I BtgI BtsI CspCI EaeI
EarI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PfoI PpuMI
PsrI PstI PvuII SanDI SfcI SfoI SmlI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AloI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanII BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstXI BstZ17I
BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI FalI FseI FspI FspAI HpaI MfeI
MluI NdeI NheI NotI NruI PacI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI