Restriction map for
pFN21AE5001
(Linear) (Six-base) MAPSORT of: KIEE5001.seq Check: 6286 from: 1 to: 3147
KIEE5001.orf
With 182 enzymes: SgfI *
December 5, 2014 12:57 ..
AccI GT'mk_AC
Cuts at: 0 888 3147
Size: 888 2259
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 889 1042 1289 2344 3147
Size: 43 846 153 247 1055 803
Fragments arranged by size:
1055 846 803 247 153 43
AflII C'TTAA_G
Cuts at: 0 2188 3147
Size: 2188 959
AflIII A'CryG_T
Cuts at: 0 329 3147
Size: 329 2818
AhdI GACnn_n'nnGTC
Cuts at: 0 1270 2576 3147
Size: 1270 1306 571
Fragments arranged by size:
1306 1270 571
AleI CACnn'nnGTG
Cuts at: 0 2431 3147
Size: 2431 716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1437 3147
Size: 1437 1710
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1358 1390 3147
Size: 1358 32 1757
Fragments arranged by size:
1757 1358 32
AlwNI CAG_nnn'CTG
Cuts at: 0 954 1377 1800 3147
Size: 954 423 423 1347
Fragments arranged by size:
1347 954 423 423
ApaLI G'TGCA_C
Cuts at: 0 2358 3147
Size: 2358 789
ApoI r'AATT_y
Cuts at: 0 32 173 755 1000 1118 1609 1873 1977
Size: 32 141 582 245 118 491 264 104
Cuts at: 1977 2895 3147
Size: 918 252
Fragments arranged by size:
918 582 491 264 252 245 141 118
104 32
AvaI C'yCGr_G
Cuts at: 0 2782 3147
Size: 2782 365
BanII G_rGCy'C
Cuts at: 0 2060 3147
Size: 2060 1087
BbsI GAAGACnn'nnnn_
Cuts at: 0 2756 3147
Size: 2756 391
BbvCI CC'TCA_GC
Cuts at: 0 1240 2116 3147
Size: 1240 876 1031
Fragments arranged by size:
1240 1031 876
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2329 2363 3147
Size: 2329 34 784
Fragments arranged by size:
2329 784 34
BglII A'GATC_T
Cuts at: 0 1278 2505 3147
Size: 1278 1227 642
Fragments arranged by size:
1278 1227 642
BlpI GC'TnA_GC
Cuts at: 0 2729 2845 3147
Size: 2729 116 302
Fragments arranged by size:
2729 302 116
Bme1580I G_kGCm'C
Cuts at: 0 917 2362 3147
Size: 917 1445 785
Fragments arranged by size:
1445 917 785
BmrI ACTGGGnnnn_n'
Cuts at: 0 322 3147
Size: 322 2825
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1874 3147
Size: 1874 1273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 931 2021 2068 2986 3147
Size: 200 731 1090 47 918 161
Fragments arranged by size:
1090 918 731 200 161 47
Bpu10I CC'TnA_GC
Cuts at: 0 1240 2116 3147
Size: 1240 876 1031
Fragments arranged by size:
1240 1031 876
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 2158 2992 3058 3147
Size: 484 1674 834 66 89
Fragments arranged by size:
1674 834 484 89 66
BsaWI w'CCGG_w
Cuts at: 0 3008 3147
Size: 3008 139
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1840 1870 1896 1926 3147
Size: 1840 30 26 30 1221
Fragments arranged by size:
1840 1221 30 30 26
BseYI C'CCAG_C
Cuts at: 0 243 1018 2703 3147
Size: 243 775 1685 444
Fragments arranged by size:
1685 775 444 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 3147
Size: 135 3012
BsiHKAI G_wGCw'C
Cuts at: 0 626 2362 2457 3147
Size: 626 1736 95 690
Fragments arranged by size:
1736 690 626 95
Bsp1286I G_dGCh'C
Cuts at: 0 626 917 2060 2362 2457 3147
Size: 626 291 1143 302 95 690
Fragments arranged by size:
1143 690 626 302 291 95
BspEI T'CCGG_A
Cuts at: 0 3008 3147
Size: 3008 139
BsrFI r'CCGG_y
Cuts at: 0 1655 3147
Size: 1655 1492
BsrGI T'GTAC_A
Cuts at: 0 2489 3147
Size: 2489 658
BstEII G'GTnAC_C
Cuts at: 0 2798 3147
Size: 2798 349
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3074 3147
Size: 3074 73
BstYI r'GATC_y
Cuts at: 0 839 957 1035 1278 1494 2256 2484 2505
Size: 839 118 78 243 216 762 228 21
Cuts at: 2505 3147
Size: 642
Fragments arranged by size:
839 762 642 243 228 216 118 78
21
BtgI C'CryG_G
Cuts at: 0 1844 3147
Size: 1844 1303
BtsI GCAGTG_nn'
Cuts at: 0 1611 3085 3147
Size: 1611 1474 62
Fragments arranged by size:
1611 1474 62
ClaI AT'CG_AT
Cuts at: 0 281 3147
Size: 281 2866
DraI TTT'AAA
Cuts at: 0 386 1146 1260 3147
Size: 386 760 114 1887
Fragments arranged by size:
1887 760 386 114
EaeI y'GGCC_r
Cuts at: 0 512 977 2045 2423 2738 3043 3098 3147
Size: 512 465 1068 378 315 305 55 49
Fragments arranged by size:
1068 512 465 378 315 305 55 49
EarI CTCTTCn'nnn_
Cuts at: 0 19 908 1682 1830 3147
Size: 19 889 774 148 1317
Fragments arranged by size:
1317 889 774 148 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 200 889 931 1042 1289 2021 2068
Size: 43 157 689 42 111 247 732 47
Cuts at: 2068 2344 2986 3147
Size: 276 642 161
Fragments arranged by size:
732 689 642 276 247 161 157 111
47 43 42
EcoO109I rG'GnC_Cy
Cuts at: 0 432 1374 3147
Size: 432 942 1773
Fragments arranged by size:
1773 942 432
EcoRI G'AATT_C
Cuts at: 0 1609 1873 3147
Size: 1609 264 1274
Fragments arranged by size:
1609 1274 264
EcoRV GAT'ATC
Cuts at: 0 2251 3147
Size: 2251 896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2313 3147
Size: 2313 834
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1124 1156 1256 1288 1479 1511 1840 1872
Size: 1124 32 100 32 191 32 329 32
Cuts at: 1872 2137 2169 2248 2280 2562 2594 3147
Size: 265 32 79 32 282 32 553
Fragments arranged by size:
1124 553 329 282 265 191 100 79
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2394 3147
Size: 2394 753
HindIII A'AGCT_T
Cuts at: 0 363 1158 3147
Size: 363 795 1989
Fragments arranged by size:
1989 795 363
HpaI GTT'AAC
Cuts at: 0 2394 3147
Size: 2394 753
MfeI C'AATT_G
Cuts at: 0 1030 3147
Size: 1030 2117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1638 1673 2053 2180 3147
Size: 1638 35 380 127 967
Fragments arranged by size:
1638 967 380 127 35
MscI TGG'CCA
Cuts at: 0 514 979 2047 2425 2740 3045 3100 3147
Size: 514 465 1068 378 315 305 55 47
Fragments arranged by size:
1068 514 465 378 315 305 55 47
MslI CAynn'nnrTG
Cuts at: 0 1892 2431 2563 3147
Size: 1892 539 132 584
Fragments arranged by size:
1892 584 539 132
MspA1I CmG'CkG
Cuts at: 0 2707 3147
Size: 2707 440
NcoI C'CATG_G
Cuts at: 0 1844 3147
Size: 1844 1303
NdeI CA'TA_TG
Cuts at: 0 1760 3147
Size: 1760 1387
NspI r_CATG'y
Cuts at: 0 333 913 1248 3147
Size: 333 580 335 1899
Fragments arranged by size:
1899 580 335 333
PciI A'CATG_T
Cuts at: 0 329 3147
Size: 329 2818
PflMI CCAn_nnn'nTGG
Cuts at: 0 954 1377 3147
Size: 954 423 1770
Fragments arranged by size:
1770 954 423
PfoI T'CCnGG_A
Cuts at: 0 177 947 1376 3147
Size: 177 770 429 1771
Fragments arranged by size:
1771 770 429 177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 677 709 3147
Size: 677 32 2438
Fragments arranged by size:
2438 677 32
PpuMI rG'GwC_Cy
Cuts at: 0 1374 3147
Size: 1374 1773
PshAI GACnn'nnGTC
Cuts at: 0 2137 3147
Size: 2137 1010
PsiI TTA'TAA
Cuts at: 0 791 1225 3147
Size: 791 434 1922
Fragments arranged by size:
1922 791 434
PstI C_TGCA'G
Cuts at: 0 350 1796 3147
Size: 350 1446 1351
Fragments arranged by size:
1446 1351 350
PvuII CAG'CTG
Cuts at: 0 2707 3147
Size: 2707 440
SapI GCTCTTCn'nnn_
Cuts at: 0 1682 3147
Size: 1682 1465
SfcI C'TryA_G
Cuts at: 0 346 835 1352 1792 3120 3147
Size: 346 489 517 440 1328 27
Fragments arranged by size:
1328 517 489 440 346 27
SmlI C'TyrA_G
Cuts at: 0 463 2173 2188 2782 2971 3037 3147
Size: 463 1710 15 594 189 66 110
Fragments arranged by size:
1710 594 463 189 110 66 15
StyI C'CwwG_G
Cuts at: 0 72 1490 1844 3147
Size: 72 1418 354 1303
Fragments arranged by size:
1418 1303 354 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2728 3080 3147
Size: 2728 352 67
Fragments arranged by size:
2728 352 67
TatI w'GTAC_w
Cuts at: 0 2489 3147
Size: 2489 658
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1896 1928 3147
Size: 1896 32 1219
Fragments arranged by size:
1896 1219 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1597 1842 3147
Size: 1597 245 1305
Fragments arranged by size:
1597 1305 245
XhoI C'TCGA_G
Cuts at: 0 2782 3147
Size: 2782 365
XmnI GAAnn'nnTTC
Cuts at: 0 1691 3147
Size: 1691 1456
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AleI AlfI AloI
AlwNI ApaLI ApoI AvaI BanII BbsI BbvCI BcgI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrFI
BsrGI BstEII BstXI BstYI BtgI BtsI ClaI DraI
EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I
NcoI NdeI NspI PciI PflMI PfoI PpiI PpuMI
PshAI PsiI PstI PvuII SapI SfcI SmlI StyI
TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BciVI
BclI BfrBI BglI BmgBI BmtI BsaI BsaAI BsaBI
BsaHI BseRI BsiEI BsiWI BsmI BsmBI BspHI BspMI
BsrBI BsrDI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
FseI FspI FspAI HaeII KasI KpnI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XmaI ZraI