Restriction map for
pFN21AE4596
(Linear) (Six-base) MAPSORT of: KIEE4596.seq Check: 5269 from: 1 to: 3738
KIEE4596.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AccI GT'mk_AC
Cuts at: 0 2417 3738
Size: 2417 1321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 1935 2527 2747 2814 3094 3738
Size: 1429 506 592 220 67 280 644
Fragments arranged by size:
1429 644 592 506 280 220 67
AfeI AGC'GCT
Cuts at: 0 1816 3738
Size: 1816 1922
AflIII A'CryG_T
Cuts at: 0 1687 1868 3408 3738
Size: 1687 181 1540 330
Fragments arranged by size:
1687 1540 330 181
AleI CACnn'nnGTG
Cuts at: 0 2791 3738
Size: 2791 947
AlwNI CAG_nnn'CTG
Cuts at: 0 339 616 1863 2355 2674 2901 3072 3606
Size: 339 277 1247 492 319 227 171 534
Cuts at: 3606 3738
Size: 132
Fragments arranged by size:
1247 534 492 339 319 277 227 171
132
ApaI G_GGCC'C
Cuts at: 0 1639 3006 3531 3738
Size: 1639 1367 525 207
Fragments arranged by size:
1639 1367 525 207
ApaLI G'TGCA_C
Cuts at: 0 1900 3738
Size: 1900 1838
ApoI r'AATT_y
Cuts at: 0 2851 3478 3495 3738
Size: 2851 627 17 243
Fragments arranged by size:
2851 627 243 17
AvaI C'yCGr_G
Cuts at: 0 755 1526 1724 2170 3530 3738
Size: 755 771 198 446 1360 208
Fragments arranged by size:
1360 771 755 446 208 198
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 788 821 3738
Size: 788 33 2917
Fragments arranged by size:
2917 788 33
BamHI G'GATC_C
Cuts at: 0 255 2862 3738
Size: 255 2607 876
Fragments arranged by size:
2607 876 255
BanI G'GyrC_C
Cuts at: 0 2137 2682 3667 3738
Size: 2137 545 985 71
Fragments arranged by size:
2137 985 545 71
BanII G_rGCy'C
Cuts at: 0 71 114 288 1508 1639 1880 2999 3006
Size: 71 43 174 1220 131 241 1119 7
Cuts at: 3006 3036 3281 3358 3531 3563 3738
Size: 30 245 77 173 32 175
Fragments arranged by size:
1220 1119 245 241 175 174 173 131
77 71 43 32 30 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 256 779 3738
Size: 256 523 2959
Fragments arranged by size:
2959 523 256
BbvCI CC'TCA_GC
Cuts at: 0 518 3738
Size: 518 3220
BciVI GTATCCnnnnn_n'
Cuts at: 0 464 816 3738
Size: 464 352 2922
Fragments arranged by size:
2922 464 352
BglI GCCn_nnn'nGGC
Cuts at: 0 414 1835 2730 3738
Size: 414 1421 895 1008
Fragments arranged by size:
1421 1008 895 414
BglII A'GATC_T
Cuts at: 0 156 495 543 1421 2816 3738
Size: 156 339 48 878 1395 922
Fragments arranged by size:
1395 922 878 339 156 48
Bme1580I G_kGCm'C
Cuts at: 0 86 153 1457 1639 1695 1904 2171 2687
Size: 86 67 1304 182 56 209 267 516
Cuts at: 2687 3006 3217 3531 3738
Size: 319 211 314 207
Fragments arranged by size:
1304 516 319 314 267 211 209 207
182 86 67 56
BmrI ACTGGGnnnn_n'
Cuts at: 0 96 283 349 420 1689 2220 3060 3738
Size: 96 187 66 71 1269 531 840 678
Fragments arranged by size:
1269 840 678 531 187 96 71 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1764 2337 3738
Size: 1764 573 1401
Fragments arranged by size:
1764 1401 573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 98 128 382 395 593 1270 1480
Size: 17 81 30 254 13 198 677 210
Cuts at: 1480 2743 3034 3738
Size: 1263 291 704
Fragments arranged by size:
1263 704 677 291 254 210 198 81
30 17 13
Bpu10I CC'TnA_GC
Cuts at: 0 518 631 1093 2915 3738
Size: 518 113 462 1822 823
Fragments arranged by size:
1822 823 518 462 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1402 1912 3376 3738
Size: 1402 510 1464 362
Fragments arranged by size:
1464 1402 510 362
BsaI GGTCTCn'nnnn_
Cuts at: 0 269 1981 2452 2589 3738
Size: 269 1712 471 137 1149
Fragments arranged by size:
1712 1149 471 269 137
BsaAI yAC'GTr
Cuts at: 0 1690 2240 3201 3738
Size: 1690 550 961 537
Fragments arranged by size:
1690 961 550 537
BsaBI GATnn'nnATC
Cuts at: 0 887 3738
Size: 887 2851
BsaHI Gr'CG_yC
Cuts at: 0 863 3738
Size: 863 2875
BsaWI w'CCGG_w
Cuts at: 0 1170 3738
Size: 1170 2568
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 275 305 597 627 853 883 2041 2071
Size: 275 30 292 30 226 30 1158 30
Cuts at: 2071 3644 3674 3738
Size: 1573 30 64
Fragments arranged by size:
1573 1158 292 275 226 64 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 526 535 538 1327 1544 2316 2404 2577
Size: 526 9 3 789 217 772 88 173
Cuts at: 2577 3031 3259 3331 3738
Size: 454 228 72 407
Fragments arranged by size:
789 772 526 454 407 228 217 173
88 72 9 3
BseYI C'CCAG_C
Cuts at: 0 195 279 902 2213 2376 2438 2462 2925
Size: 195 84 623 1311 163 62 24 463
Cuts at: 2925 3367 3663 3738
Size: 442 296 75
Fragments arranged by size:
1311 623 463 442 296 195 163 84
75 62 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1625 1849 2113 2170 3738
Size: 943 682 224 264 57 1568
Fragments arranged by size:
1568 943 682 264 224 57
BsiHKAI G_wGCw'C
Cuts at: 0 71 114 227 747 1466 1508 1880 1904
Size: 71 43 113 520 719 42 372 24
Cuts at: 1904 2798 3185 3281 3304 3738
Size: 894 387 96 23 434
Fragments arranged by size:
894 719 520 434 387 372 113 96
71 43 42 24 23
BsmI GAATG_Cn'
Cuts at: 0 3095 3596 3738
Size: 3095 501 142
Fragments arranged by size:
3095 501 142
Bsp1286I G_dGCh'C
Cuts at: 0 71 86 114 153 227 288 747 1457
Size: 71 15 28 39 74 61 459 710
Cuts at: 1457 1466 1508 1639 1695 1880 1904 2171 2687
Size: 9 42 131 56 185 24 267 516
Cuts at: 2687 2798 2999 3006 3036 3185 3217 3281 3304
Size: 111 201 7 30 149 32 64 23
Cuts at: 3304 3358 3531 3563 3738
Size: 54 173 32 175
Fragments arranged by size:
710 516 459 267 201 185 175 173
149 131 111 74 71 64 61 56
54 42 39 32 32 30 28 24
23 15 9 7
BspEI T'CCGG_A
Cuts at: 0 1170 3738
Size: 1170 2568
BspHI T'CATG_A
Cuts at: 0 1190 3738
Size: 1190 2548
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2081 2192 2429 2969 3738
Size: 2081 111 237 540 769
Fragments arranged by size:
2081 769 540 237 111
BsrBI CCG'CTC
Cuts at: 0 3114 3738
Size: 3114 624
BsrDI GCAATG_nn'
Cuts at: 0 1034 3142 3738
Size: 1034 2108 596
Fragments arranged by size:
2108 1034 596
BsrFI r'CCGG_y
Cuts at: 0 404 3738
Size: 404 3334
BsrGI T'GTAC_A
Cuts at: 0 1158 1220 3042 3738
Size: 1158 62 1822 696
Fragments arranged by size:
1822 1158 696 62
BssSI C'ACGA_G
Cuts at: 0 2701 3738
Size: 2701 1037
BstXI CCAn_nnnn'nTGG
Cuts at: 0 858 1041 1606 2376 3358 3738
Size: 858 183 565 770 982 380
Fragments arranged by size:
982 858 770 565 380 183
BstYI r'GATC_y
Cuts at: 0 36 156 255 495 543 1202 1421 1796
Size: 36 120 99 240 48 659 219 375
Cuts at: 1796 2816 2862 3738
Size: 1020 46 876
Fragments arranged by size:
1020 876 659 375 240 219 120 99
48 46 36
Bsu36I CC'TnA_GG
Cuts at: 0 1749 2278 3314 3575 3738
Size: 1749 529 1036 261 163
Fragments arranged by size:
1749 1036 529 261 163
BtgI C'CryG_G
Cuts at: 0 62 132 1650 1703 1833 1956 1997 2747
Size: 62 70 1518 53 130 123 41 750
Cuts at: 2747 3738
Size: 991
Fragments arranged by size:
1518 991 750 130 123 70 62 53
41
BtsI GCAGTG_nn'
Cuts at: 0 695 747 850 2672 2885 3625 3738
Size: 695 52 103 1822 213 740 113
Fragments arranged by size:
1822 740 695 213 113 103 52
DraIII CAC_nnn'GTG
Cuts at: 0 2748 3738
Size: 2748 990
EaeI y'GGCC_r
Cuts at: 0 463 2406 3422 3719 3738
Size: 463 1943 1016 297 19
Fragments arranged by size:
1943 1016 463 297 19
EarI CTCTTCn'nnn_
Cuts at: 0 404 1783 1810 1954 2293 3738
Size: 404 1379 27 144 339 1445
Fragments arranged by size:
1445 1379 404 339 144 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2130 2221 3738
Size: 2130 91 1517
Fragments arranged by size:
2130 1517 91
EcoICRI GAG'CTC
Cuts at: 0 69 112 1506 1878 3279 3738
Size: 69 43 1394 372 1401 459
Fragments arranged by size:
1401 1394 459 372 69 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 98 128 382 395 593 1270 1429
Size: 17 81 30 254 13 198 677 159
Cuts at: 1429 1480 1935 2527 2743 2747 2814 3034 3094
Size: 51 455 592 216 4 67 220 60
Cuts at: 3094 3738
Size: 644
Fragments arranged by size:
677 644 592 455 254 220 216 198
159 81 67 60 51 30 17 13
4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1966 3394 3738
Size: 1966 1428 344
Fragments arranged by size:
1966 1428 344
EcoO109I rG'GnC_Cy
Cuts at: 0 706 819 1089 1245 1635 1636 1860 2070
Size: 706 113 270 156 390 1 224 210
Cuts at: 2070 2121 2352 2649 2731 3569 3579 3738
Size: 51 231 297 82 838 10 159
Fragments arranged by size:
838 706 390 297 270 231 224 210
159 156 113 82 51 10 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 565 2322 3738
Size: 565 1757 1416
Fragments arranged by size:
1757 1416 565
FspI TGC'GCA
Cuts at: 0 2044 3738
Size: 2044 1694
HaeII r_GCGC'y
Cuts at: 0 1818 3606 3738
Size: 1818 1788 132
Fragments arranged by size:
1818 1788 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 247 275 279 307 817 849 853 885
Size: 247 28 4 28 510 32 4 32
Cuts at: 885 3738
Size: 2853
Fragments arranged by size:
2853 510 247 32 32 28 28 4
4
HincII GTy'rAC
Cuts at: 0 2418 3738
Size: 2418 1320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 469 623 628 3113 3738
Size: 323 146 154 5 2485 625
Fragments arranged by size:
2485 625 323 154 146 5
MscI TGG'CCA
Cuts at: 0 465 2408 3424 3738
Size: 465 1943 1016 314
Fragments arranged by size:
1943 1016 465 314
MslI CAynn'nnrTG
Cuts at: 0 856 2197 2791 3738
Size: 856 1341 594 947
Fragments arranged by size:
1341 947 856 594
MspA1I CmG'CkG
Cuts at: 0 802 1164 1561 1652 1733 1851 2217 2438
Size: 802 362 397 91 81 118 366 221
Cuts at: 2438 3593 3685 3738
Size: 1155 92 53
Fragments arranged by size:
1155 802 397 366 362 221 118 92
91 81 53
NaeI GCC'GGC
Cuts at: 0 406 3738
Size: 406 3332
NcoI C'CATG_G
Cuts at: 0 132 1997 3738
Size: 132 1865 1741
Fragments arranged by size:
1865 1741 132
NgoMIV G'CCGG_C
Cuts at: 0 404 3738
Size: 404 3334
NspI r_CATG'y
Cuts at: 0 1872 2013 3540 3738
Size: 1872 141 1527 198
Fragments arranged by size:
1872 1527 198 141
PasI CC'CwG_GG
Cuts at: 0 1497 2080 3192 3358 3738
Size: 1497 583 1112 166 380
Fragments arranged by size:
1497 1112 583 380 166
PciI A'CATG_T
Cuts at: 0 1868 3738
Size: 1868 1870
PflMI CCAn_nnn'nTGG
Cuts at: 0 339 616 2748 3471 3738
Size: 339 277 2132 723 267
Fragments arranged by size:
2132 723 339 277 267
PfoI T'CCnGG_A
Cuts at: 0 615 1109 2066 2894 3738
Size: 615 494 957 828 844
Fragments arranged by size:
957 844 828 615 494
PmlI CAC'GTG
Cuts at: 0 1690 3738
Size: 1690 2048
PpuMI rG'GwC_Cy
Cuts at: 0 706 1245 1860 2070 2649 3569 3738
Size: 706 539 615 210 579 920 169
Fragments arranged by size:
920 706 615 579 539 210 169
PspOMI G'GGCC_C
Cuts at: 0 1635 3002 3527 3738
Size: 1635 1367 525 211
Fragments arranged by size:
1635 1367 525 211
PstI C_TGCA'G
Cuts at: 0 164 742 1008 1814 2222 2679 2966 3738
Size: 164 578 266 806 408 457 287 772
Fragments arranged by size:
806 772 578 457 408 287 266 164
PvuII CAG'CTG
Cuts at: 0 802 1164 1561 1851 2217 2438 3738
Size: 802 362 397 290 366 221 1300
Fragments arranged by size:
1300 802 397 366 362 290 221
SacI G_AGCT'C
Cuts at: 0 71 114 1508 1880 3281 3738
Size: 71 43 1394 372 1401 457
Fragments arranged by size:
1401 1394 457 372 71 43
SacII CC_GC'GG
Cuts at: 0 1653 3738
Size: 1653 2085
SalI G'TCGA_C
Cuts at: 0 2416 3738
Size: 2416 1322
SanDI GG'GwC_CC
Cuts at: 0 3569 3738
Size: 3569 169
SapI GCTCTTCn'nnn_
Cuts at: 0 1783 1954 3738
Size: 1783 171 1784
Fragments arranged by size:
1784 1783 171
SexAI A'CCwGG_T
Cuts at: 0 561 3738
Size: 561 3177
SfcI C'TryA_G
Cuts at: 0 160 304 738 1004 1810 2218 2578 2675
Size: 160 144 434 266 806 408 360 97
Cuts at: 2675 2962 3738
Size: 287 776
Fragments arranged by size:
806 776 434 408 360 287 266 160
144 97
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 414 3738
Size: 414 3324
SmaI CCC'GGG
Cuts at: 0 1726 3532 3738
Size: 1726 1806 206
Fragments arranged by size:
1806 1726 206
SmlI C'TyrA_G
Cuts at: 0 1381 1927 3391 3738
Size: 1381 546 1464 347
Fragments arranged by size:
1464 1381 546 347
SphI G_CATG'C
Cuts at: 0 3540 3738
Size: 3540 198
SspI AAT'ATT
Cuts at: 0 2431 3738
Size: 2431 1307
StuI AGG'CCT
Cuts at: 0 1177 1747 1754 3738
Size: 1177 570 7 1984
Fragments arranged by size:
1984 1177 570 7
StyI C'CwwG_G
Cuts at: 0 132 1997 2789 3738
Size: 132 1865 792 949
Fragments arranged by size:
1865 949 792 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 335 649 1885 2939 3655 3738
Size: 335 314 1236 1054 716 83
Fragments arranged by size:
1236 1054 716 335 314 83
TatI w'GTAC_w
Cuts at: 0 1158 1220 3042 3738
Size: 1158 62 1822 696
Fragments arranged by size:
1822 1158 696 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1237 1269 2041 2073 2516 2548 3644 3676
Size: 1237 32 772 32 443 32 1096 32
Cuts at: 3676 3738
Size: 62
Fragments arranged by size:
1237 1096 772 443 62 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 979 2950 3738
Size: 979 1971 788
Fragments arranged by size:
1971 979 788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 914 960 3451 3738
Size: 914 46 2491 287
Fragments arranged by size:
2491 914 287 46
XmaI C'CCGG_G
Cuts at: 0 1724 3530 3738
Size: 1724 1806 208
Fragments arranged by size:
1806 1724 208
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BciVI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SacII SalI SanDI SapI SexAI SfcI SfiI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BbeI BcgI BclI
BfrBI BlpI BmgBI BmtI BsiEI BsiWI BsmBI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EcoRI EcoRV FseI FspAI HindIII HpaI
KasI KpnI MfeI MluI NarI NdeI NheI NotI
NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SbfI ScaI SfoI SgfI SgrAI
SnaBI SpeI SrfI SwaI XbaI XhoI XmnI ZraI