Restriction map for
pFN21AE4594
(Linear) (Six-base) MAPSORT of: KIEE4594.seq Check: 8688 from: 1 to: 3705
KIEE4594.orf
With 182 enzymes: SgfI *
March 22, 2012 18:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1663 3705
Size: 1663 2042
AccI GT'mk_AC
Cuts at: 0 748 1169 3705
Size: 748 421 2536
Fragments arranged by size:
2536 748 421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1005 1280 2052 3705
Size: 1005 275 772 1653
Fragments arranged by size:
1653 1005 772 275
AfeI AGC'GCT
Cuts at: 0 3609 3705
Size: 3609 96
AhdI GACnn_n'nnGTC
Cuts at: 0 1907 3537 3705
Size: 1907 1630 168
Fragments arranged by size:
1907 1630 168
AleI CACnn'nnGTG
Cuts at: 0 370 3705
Size: 370 3335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 137 2545 3705
Size: 137 2408 1160
Fragments arranged by size:
2408 1160 137
AlwNI CAG_nnn'CTG
Cuts at: 0 3201 3417 3705
Size: 3201 216 288
Fragments arranged by size:
3201 288 216
ApaLI G'TGCA_C
Cuts at: 0 1650 3705
Size: 1650 2055
ApoI r'AATT_y
Cuts at: 0 1476 1816 3705
Size: 1476 340 1889
Fragments arranged by size:
1889 1476 340
AvaI C'yCGr_G
Cuts at: 0 2569 2576 3156 3329 3400 3705
Size: 2569 7 580 173 71 305
Fragments arranged by size:
2569 580 305 173 71 7
BamHI G'GATC_C
Cuts at: 0 1094 1857 3705
Size: 1094 763 1848
Fragments arranged by size:
1848 1094 763
BanI G'GyrC_C
Cuts at: 0 1104 1191 3547 3705
Size: 1104 87 2356 158
Fragments arranged by size:
2356 1104 158 87
BanII G_rGCy'C
Cuts at: 0 660 2429 2576 2618 3705
Size: 660 1769 147 42 1087
Fragments arranged by size:
1769 1087 660 147 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 1011 1952 2058 2060 3705
Size: 253 758 941 106 2 1645
Fragments arranged by size:
1645 941 758 253 106 2
BbvCI CC'TCA_GC
Cuts at: 0 1225 2207 2513 2836 3705
Size: 1225 982 306 323 869
Fragments arranged by size:
1225 982 869 323 306
BciVI GTATCCnnnnn_n'
Cuts at: 0 160 3705
Size: 160 3545
BclI T'GATC_A
Cuts at: 0 2756 3705
Size: 2756 949
Bme1580I G_kGCm'C
Cuts at: 0 1654 2175 3705
Size: 1654 521 1530
Fragments arranged by size:
1654 1530 521
BmgBI CAC'GTC
Cuts at: 0 2271 3143 3705
Size: 2271 872 562
Fragments arranged by size:
2271 872 562
BmrI ACTGGGnnnn_n'
Cuts at: 0 1560 3705
Size: 1560 2145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 280 3705
Size: 280 3425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 382 844 1084 1680 3259 3583 3705
Size: 250 132 462 240 596 1579 324 122
Fragments arranged by size:
1579 596 462 324 250 240 132 122
Bpu10I CC'TnA_GC
Cuts at: 0 1225 1627 2207 2513 2802 2836 3705
Size: 1225 402 580 306 289 34 869
Fragments arranged by size:
1225 869 580 402 306 289 34
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 937 3705
Size: 646 291 2768
Fragments arranged by size:
2768 646 291
BsaI GGTCTCn'nnnn_
Cuts at: 0 3295 3705
Size: 3295 410
BsaAI yAC'GTr
Cuts at: 0 460 3489 3705
Size: 460 3029 216
Fragments arranged by size:
3029 460 216
BsaWI w'CCGG_w
Cuts at: 0 2744 2968 3705
Size: 2744 224 737
Fragments arranged by size:
2744 737 224
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1778 1808 3277 3307 3705
Size: 1778 30 1469 30 398
Fragments arranged by size:
1778 1469 398 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1799 2024 2195 2626 2913 3003 3024 3705
Size: 1799 225 171 431 287 90 21 681
Fragments arranged by size:
1799 681 431 287 225 171 90 21
BseYI C'CCAG_C
Cuts at: 0 2365 2369 2716 3176 3705
Size: 2365 4 347 460 529
Fragments arranged by size:
2365 529 460 347 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 319 2505 2848 3523 3705
Size: 73 246 2186 343 675 182
Fragments arranged by size:
2186 675 343 246 182 73
BsiEI CG_ry'CG
Cuts at: 0 1492 3705
Size: 1492 2213
BsiHKAI G_wGCw'C
Cuts at: 0 322 710 1654 1748 2429 2618 3705
Size: 322 388 944 94 681 189 1087
Fragments arranged by size:
1087 944 681 388 322 189 94
BsmI GAATG_Cn'
Cuts at: 0 195 597 3093 3705
Size: 195 402 2496 612
Fragments arranged by size:
2496 612 402 195
Bsp1286I G_dGCh'C
Cuts at: 0 322 660 710 1654 1748 2175 2429 2576
Size: 322 338 50 944 94 427 254 147
Cuts at: 2576 2618 3705
Size: 42 1087
Fragments arranged by size:
1087 944 427 338 322 254 147 94
50 42
BspEI T'CCGG_A
Cuts at: 0 2744 3705
Size: 2744 961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1663 2537 3705
Size: 1663 874 1168
Fragments arranged by size:
1663 1168 874
BsrBI CCG'CTC
Cuts at: 0 3186 3705
Size: 3186 519
BsrDI GCAATG_nn'
Cuts at: 0 2657 3705
Size: 2657 1048
BsrFI r'CCGG_y
Cuts at: 0 2440 3407 3705
Size: 2440 967 298
Fragments arranged by size:
2440 967 298
BsrGI T'GTAC_A
Cuts at: 0 1217 2339 3705
Size: 1217 1122 1366
Fragments arranged by size:
1366 1217 1122
BssSI C'ACGA_G
Cuts at: 0 2953 3705
Size: 2953 752
BstBI TT'CG_AA
Cuts at: 0 962 2311 3705
Size: 962 1349 1394
Fragments arranged by size:
1394 1349 962
BstEII G'GTnAC_C
Cuts at: 0 2400 3705
Size: 2400 1305
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2781 3705
Size: 2781 924
BstYI r'GATC_y
Cuts at: 0 1094 1857 3269 3705
Size: 1094 763 1412 436
Fragments arranged by size:
1412 1094 763 436
Bsu36I CC'TnA_GG
Cuts at: 0 450 3705
Size: 450 3255
BtgI C'CryG_G
Cuts at: 0 660 2555 2774 3242 3285 3551 3705
Size: 660 1895 219 468 43 266 154
Fragments arranged by size:
1895 660 468 266 219 154 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 215 3705
Size: 215 3490
BtsI GCAGTG_nn'
Cuts at: 0 708 812 3093 3659 3705
Size: 708 104 2281 566 46
Fragments arranged by size:
2281 708 566 104 46
ClaI AT'CG_AT
Cuts at: 0 1268 3299 3705
Size: 1268 2031 406
Fragments arranged by size:
2031 1268 406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3635 3670 3705
Size: 3635 35 35
Fragments arranged by size:
3635 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1377 2958 3705
Size: 1377 1581 747
Fragments arranged by size:
1581 1377 747
DrdI GACnn_nn'nnGTC
Cuts at: 0 2062 3705
Size: 2062 1643
EaeI y'GGCC_r
Cuts at: 0 155 287 611 2693 2771 3509 3705
Size: 155 132 324 2082 78 738 196
Fragments arranged by size:
2082 738 324 196 155 132 78
EarI CTCTTCn'nnn_
Cuts at: 0 424 964 1139 2804 3705
Size: 424 540 175 1665 901
Fragments arranged by size:
1665 901 540 424 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1087 3705
Size: 1087 2618
EcoICRI GAG'CTC
Cuts at: 0 2427 2616 3705
Size: 2427 189 1089
Fragments arranged by size:
2427 1089 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 382 844 1005 1084 1280 1680 2052
Size: 250 132 462 161 79 196 400 372
Cuts at: 2052 3259 3583 3705
Size: 1207 324 122
Fragments arranged by size:
1207 462 400 372 324 250 196 161
132 122 79
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1659 2221 2512 2671 3705
Size: 1659 562 291 159 1034
Fragments arranged by size:
1659 1034 562 291 159
EcoO109I rG'GnC_Cy
Cuts at: 0 165 918 1059 2021 2362 2505 3237 3705
Size: 165 753 141 962 341 143 732 468
Fragments arranged by size:
962 753 732 468 341 165 143 141
EcoRV GAT'ATC
Cuts at: 0 1939 3705
Size: 1939 1766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1993 3705
Size: 1993 1712
FspI TGC'GCA
Cuts at: 0 3092 3205 3705
Size: 3092 113 500
Fragments arranged by size:
3092 500 113
FspAI rTGC'GCAy
Cuts at: 0 3092 3705
Size: 3092 613
HaeII r_GCGC'y
Cuts at: 0 3611 3705
Size: 3611 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 397 429 1422 1454 1667 1699
Size: 243 32 122 32 993 32 213 32
Cuts at: 1699 1893 1925 2010 2042 3155 3187 3498 3523
Size: 194 32 85 32 1113 32 311 25
Cuts at: 3523 3530 3555 3705
Size: 7 25 150
Fragments arranged by size:
1113 993 311 243 213 194 150 122
85 32 32 32 32 32 32 32
25 25 7
HincII GTy'rAC
Cuts at: 0 749 3705
Size: 749 2956
HindIII A'AGCT_T
Cuts at: 0 1453 3705
Size: 1453 2252
MfeI C'AATT_G
Cuts at: 0 632 3542 3705
Size: 632 2910 163
Fragments arranged by size:
2910 632 163
MscI TGG'CCA
Cuts at: 0 289 613 2695 2773 3511 3705
Size: 289 324 2082 78 738 194
Fragments arranged by size:
2082 738 324 289 194 78
MslI CAynn'nnrTG
Cuts at: 0 370 1445 1505 2764 3433 3460 3487 3705
Size: 370 1075 60 1259 669 27 27 218
Fragments arranged by size:
1259 1075 669 370 218 60 27 27
MspA1I CmG'CkG
Cuts at: 0 1258 2369 3414 3519 3705
Size: 1258 1111 1045 105 186
Fragments arranged by size:
1258 1111 1045 186 105
NaeI GCC'GGC
Cuts at: 0 2442 3705
Size: 2442 1263
NcoI C'CATG_G
Cuts at: 0 660 3551 3705
Size: 660 2891 154
Fragments arranged by size:
2891 660 154
NgoMIV G'CCGG_C
Cuts at: 0 2440 3705
Size: 2440 1265
PflMI CCAn_nnn'nTGG
Cuts at: 0 2958 3705
Size: 2958 747
PfoI T'CCnGG_A
Cuts at: 0 581 3705
Size: 581 3124
PmlI CAC'GTG
Cuts at: 0 3489 3705
Size: 3489 216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 941 973 3705
Size: 941 32 2732
Fragments arranged by size:
2732 941 32
PpuMI rG'GwC_Cy
Cuts at: 0 918 2021 2362 2505 3237 3705
Size: 918 1103 341 143 732 468
Fragments arranged by size:
1103 918 732 468 341 143
PshAI GACnn'nnGTC
Cuts at: 0 672 3705
Size: 672 3033
PsiI TTA'TAA
Cuts at: 0 902 1006 3705
Size: 902 104 2699
Fragments arranged by size:
2699 902 104
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1160 1192 3705
Size: 1160 32 2513
Fragments arranged by size:
2513 1160 32
PvuII CAG'CTG
Cuts at: 0 2369 3414 3519 3705
Size: 2369 1045 105 186
Fragments arranged by size:
2369 1045 186 105
SacI G_AGCT'C
Cuts at: 0 2429 2618 3705
Size: 2429 189 1087
Fragments arranged by size:
2429 1087 189
SalI G'TCGA_C
Cuts at: 0 747 3705
Size: 747 2958
SanDI GG'GwC_CC
Cuts at: 0 2362 3237 3705
Size: 2362 875 468
Fragments arranged by size:
2362 875 468
SapI GCTCTTCn'nnn_
Cuts at: 0 964 3705
Size: 964 2741
SexAI A'CCwGG_T
Cuts at: 0 3533 3705
Size: 3533 172
SfcI C'TryA_G
Cuts at: 0 144 2317 3705
Size: 144 2173 1388
Fragments arranged by size:
2173 1388 144
SmaI CCC'GGG
Cuts at: 0 2578 3705
Size: 2578 1127
SmlI C'TyrA_G
Cuts at: 0 625 952 3705
Size: 625 327 2753
Fragments arranged by size:
2753 625 327
SnaBI TAC'GTA
Cuts at: 0 460 3705
Size: 460 3245
StuI AGG'CCT
Cuts at: 0 220 2483 2518 3705
Size: 220 2263 35 1187
Fragments arranged by size:
2263 1187 220 35
StyI C'CwwG_G
Cuts at: 0 215 323 614 660 1512 3551 3705
Size: 215 108 291 46 852 2039 154
Fragments arranged by size:
2039 852 291 215 154 108 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 117 1187 3705
Size: 117 1070 2518
Fragments arranged by size:
2518 1070 117
TatI w'GTAC_w
Cuts at: 0 344 1148 1217 1317 2339 3705
Size: 344 804 69 100 1022 1366
Fragments arranged by size:
1366 1022 804 344 100 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1673 1705 3277 3309 3705
Size: 1673 32 1572 32 396
Fragments arranged by size:
1673 1572 396 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 868 2724 2875 2955 3541 3558 3705
Size: 868 1856 151 80 586 17 147
Fragments arranged by size:
1856 868 586 151 147 80 17
XmaI C'CCGG_G
Cuts at: 0 2576 3705
Size: 2576 1129
XmnI GAAnn'nnTTC
Cuts at: 0 1287 2225 3705
Size: 1287 938 1480
Fragments arranged by size:
1480 1287 938
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AleI AlfI AlwNI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BciVI BclI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII MfeI MscI
MslI MspA1I NaeI NcoI NgoMIV PflMI PfoI PmlI
PpiI PpuMI PshAI PsiI PsrI PvuII SacI SalI
SanDI SapI SexAI SfcI SmaI SmlI SnaBI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AloI ApaI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BfrBI
BglI BglII BlpI BmtI BsaBI BsaHI BsiWI BsmBI
BspHI BssHII BstAPI BstZ17I DraI EagI EcoRI FseI
HpaI KasI KpnI MluI MmeI NarI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PspOMI PspXI PstI PvuI RsrII SacII SbfI ScaI
SfiI SfoI SgfI SgrAI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI ZraI