Restriction map for
pFN21AE4592
(Linear) (Six-base) MAPSORT of: KIEE4592.seq Check: 176 from: 1 to: 3573
KIEE4592.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 921 1655 2046 2106 2145 3191 3197 3573
Size: 921 734 391 60 39 1046 6 376
Fragments arranged by size:
1046 921 734 391 376 60 39 6
AccI GT'mk_AC
Cuts at: 0 1279 3573
Size: 1279 2294
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3568 3573
Size: 3568 5
AfeI AGC'GCT
Cuts at: 0 3014 3457 3573
Size: 3014 443 116
Fragments arranged by size:
3014 443 116
AflIII A'CryG_T
Cuts at: 0 3044 3573
Size: 3044 529
AgeI A'CCGG_T
Cuts at: 0 1809 3573
Size: 1809 1764
AhdI GACnn_n'nnGTC
Cuts at: 0 27 3573
Size: 27 3546
AleI CACnn'nnGTG
Cuts at: 0 835 3573
Size: 835 2738
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2810 2842 3573
Size: 2810 32 731
Fragments arranged by size:
2810 731 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3220 3483 3573
Size: 3220 263 90
Fragments arranged by size:
3220 263 90
ApaI G_GGCC'C
Cuts at: 0 68 463 636 739 966 1048 1404 1529
Size: 68 395 173 103 227 82 356 125
Cuts at: 1529 1783 1910 2090 2108 2569 2993 3573
Size: 254 127 180 18 461 424 580
Fragments arranged by size:
580 461 424 395 356 254 227 180
173 127 125 103 82 68 18
ApaLI G'TGCA_C
Cuts at: 0 3229 3573
Size: 3229 344
AvaI C'yCGr_G
Cuts at: 0 68 170 1938 1978 2212 2739 2792 3573
Size: 68 102 1768 40 234 527 53 781
Fragments arranged by size:
1768 781 527 234 102 68 53 40
AvrII C'CTAG_G
Cuts at: 0 361 3573
Size: 361 3212
BamHI G'GATC_C
Cuts at: 0 2907 3275 3573
Size: 2907 368 298
Fragments arranged by size:
2907 368 298
BanI G'GyrC_C
Cuts at: 0 1791 1812 2223 3573
Size: 1791 21 411 1350
Fragments arranged by size:
1791 1350 411 21
BanII G_rGCy'C
Cuts at: 0 68 463 599 636 739 966 1048 1404
Size: 68 395 136 37 103 227 82 356
Cuts at: 1404 1529 1783 1910 1932 2068 2090 2108 2221
Size: 125 254 127 22 136 22 18 113
Cuts at: 2221 2371 2569 2747 2993 3573
Size: 150 198 178 246 580
Fragments arranged by size:
580 395 356 254 246 227 198 178
150 136 136 127 125 113 103 82
68 37 22 22 18
BbeI G_GCGC'C
Cuts at: 0 1795 3573
Size: 1795 1778
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 3573
Size: 10 3563
BbvCI CC'TCA_GC
Cuts at: 0 2195 3573
Size: 2195 1378
BciVI GTATCCnnnnn_n'
Cuts at: 0 1963 3573
Size: 1963 1610
BfrBI ATG'CAT
Cuts at: 0 937 3573
Size: 937 2636
BglI GCCn_nnn'nGGC
Cuts at: 0 166 1685 1694 1864 2954 3573
Size: 166 1519 9 170 1090 619
Fragments arranged by size:
1519 1090 619 170 166 9
BglII A'GATC_T
Cuts at: 0 224 3573
Size: 224 3349
BlpI GC'TnA_GC
Cuts at: 0 3056 3446 3573
Size: 3056 390 127
Fragments arranged by size:
3056 390 127
Bme1580I G_kGCm'C
Cuts at: 0 68 463 636 670 739 966 1048 1404
Size: 68 395 173 34 69 227 82 356
Cuts at: 1404 1529 1783 1910 2090 2108 2228 2569 2576
Size: 125 254 127 180 18 120 341 7
Cuts at: 2576 2621 2723 2993 3233 3573
Size: 45 102 270 240 340
Fragments arranged by size:
395 356 341 340 270 254 240 227
180 173 127 125 120 102 82 69
68 45 34 18 7
BmgBI CAC'GTC
Cuts at: 0 119 392 3573
Size: 119 273 3181
Fragments arranged by size:
3181 273 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 392 1398 1406 2563 3573
Size: 392 1006 8 1157 1010
Fragments arranged by size:
1157 1010 1006 392 8
BmtI G_CTAG'C
Cuts at: 0 545 3573
Size: 545 3028
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 201 243 447 3573
Size: 201 42 204 3126
Fragments arranged by size:
3126 204 201 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 818 1082 1559 1579 1685 2383 2627
Size: 125 693 264 477 20 106 698 244
Cuts at: 2627 3016 3573
Size: 389 557
Fragments arranged by size:
698 693 557 477 389 264 244 125
106 20
Bpu10I CC'TnA_GC
Cuts at: 0 2164 2195 2528 3573
Size: 2164 31 333 1045
Fragments arranged by size:
2164 1045 333 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1724 1769 2824 3573
Size: 1724 45 1055 749
Fragments arranged by size:
1724 1055 749 45
BsaI GGTCTCn'nnnn_
Cuts at: 0 241 915 1619 2200 2816 3266 3573
Size: 241 674 704 581 616 450 307
Fragments arranged by size:
704 674 616 581 450 307 241
BsaAI yAC'GTr
Cuts at: 0 2718 3573
Size: 2718 855
BsaHI Gr'CG_yC
Cuts at: 0 1792 3573
Size: 1792 1781
BsaWI w'CCGG_w
Cuts at: 0 1809 2544 3573
Size: 1809 735 1029
Fragments arranged by size:
1809 1029 735
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1041 1071 1305 1335 2810 2840 3148 3178
Size: 1041 30 234 30 1475 30 308 30
Cuts at: 3178 3573
Size: 395
Fragments arranged by size:
1475 1041 395 308 234 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 51 235 259 614 1146 1164 1325 1523
Size: 51 184 24 355 532 18 161 198
Cuts at: 1523 2212 3573
Size: 689 1361
Fragments arranged by size:
1361 689 532 355 198 184 161 51
24 18
BseYI C'CCAG_C
Cuts at: 0 462 1197 1216 2229 2272 3022 3285 3573
Size: 462 735 19 1013 43 750 263 288
Fragments arranged by size:
1013 750 735 462 288 263 43 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2944 3169 3175 3310 3487 3573
Size: 2944 225 6 135 177 86
Fragments arranged by size:
2944 225 177 135 86 6
BsiHKAI G_wGCw'C
Cuts at: 0 810 2544 3233 3573
Size: 810 1734 689 340
Fragments arranged by size:
1734 810 689 340
BsiWI C'GTAC_G
Cuts at: 0 1916 3573
Size: 1916 1657
BsmI GAATG_Cn'
Cuts at: 0 2557 3573
Size: 2557 1016
Bsp1286I G_dGCh'C
Cuts at: 0 68 463 599 636 670 739 810 966
Size: 68 395 136 37 34 69 71 156
Cuts at: 966 1048 1404 1529 1783 1910 1932 2068 2090
Size: 82 356 125 254 127 22 136 22
Cuts at: 2090 2108 2221 2228 2371 2544 2569 2576 2621
Size: 18 113 7 143 173 25 7 45
Cuts at: 2621 2723 2747 2993 3233 3573
Size: 102 24 246 240 340
Fragments arranged by size:
395 356 340 254 246 240 173 156
143 136 136 127 125 113 102 82
71 69 68 45 37 34 25 24
22 22 18 7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 908 921 1655 2046 2106 2145 3191 3197
Size: 908 13 734 391 60 39 1046 6
Cuts at: 3197 3386 3573
Size: 189 187
Fragments arranged by size:
1046 908 734 391 189 187 60 39
13 6
BsrBI CCG'CTC
Cuts at: 0 2463 3060 3573
Size: 2463 597 513
Fragments arranged by size:
2463 597 513
BsrDI GCAATG_nn'
Cuts at: 0 1210 1371 1853 3114 3573
Size: 1210 161 482 1261 459
Fragments arranged by size:
1261 1210 482 459 161
BsrFI r'CCGG_y
Cuts at: 0 1809 2190 3381 3573
Size: 1809 381 1191 192
Fragments arranged by size:
1809 1191 381 192
BsrGI T'GTAC_A
Cuts at: 0 2771 3573
Size: 2771 802
BssHII G'CGCG_C
Cuts at: 0 2333 2335 2434 3573
Size: 2333 2 99 1139
Fragments arranged by size:
2333 1139 99 2
BssSI C'ACGA_G
Cuts at: 0 2328 3328 3573
Size: 2328 1000 245
Fragments arranged by size:
2328 1000 245
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3220 3429 3483 3510 3573
Size: 3220 209 54 27 63
Fragments arranged by size:
3220 209 63 54 27
BstEII G'GTnAC_C
Cuts at: 0 1821 3573
Size: 1821 1752
BstXI CCAn_nnnn'nTGG
Cuts at: 0 837 3573
Size: 837 2736
BstYI r'GATC_y
Cuts at: 0 224 2907 3275 3573
Size: 224 2683 368 298
Fragments arranged by size:
2683 368 298 224
BstZ17I GTA'TAC
Cuts at: 0 1280 3573
Size: 1280 2293
Bsu36I CC'TnA_GG
Cuts at: 0 2109 2151 3255 3573
Size: 2109 42 1104 318
Fragments arranged by size:
2109 1104 318 42
BtgI C'CryG_G
Cuts at: 0 590 1001 1847 1862 2075 3573
Size: 590 411 846 15 213 1498
Fragments arranged by size:
1498 846 590 411 213 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2105 2563 3573
Size: 2105 458 1010
Fragments arranged by size:
2105 1010 458
BtsI GCAGTG_nn'
Cuts at: 0 132 2607 2738 2784 3484 3573
Size: 132 2475 131 46 700 89
Fragments arranged by size:
2475 700 132 131 89 46
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1836 1871 3573
Size: 1836 35 1702
Fragments arranged by size:
1836 1702 35
DraIII CAC_nnn'GTG
Cuts at: 0 3510 3573
Size: 3510 63
EaeI y'GGCC_r
Cuts at: 0 292 1344 2378 3573
Size: 292 1052 1034 1195
Fragments arranged by size:
1195 1052 1034 292
EarI CTCTTCn'nnn_
Cuts at: 0 1095 1164 1182 1713 1719 1728 1734 1836
Size: 1095 69 18 531 6 9 6 102
Cuts at: 1836 3573
Size: 1737
Fragments arranged by size:
1737 1095 531 102 69 18 9 6
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 345 3573
Size: 345 3228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 818 1082 1559 1579 1685 2383 2627
Size: 125 693 264 477 20 106 698 244
Cuts at: 2627 3016 3568 3573
Size: 389 552 5
Fragments arranged by size:
698 693 552 477 389 264 244 125
106 20 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 454 2150 3550 3573
Size: 454 1696 1400 23
Fragments arranged by size:
1696 1400 454 23
EcoO109I rG'GnC_Cy
Cuts at: 0 57 65 459 632 633 700 735 736
Size: 57 8 394 173 1 67 35 1
Cuts at: 736 962 1044 1321 1378 1400 1526 1622 1687
Size: 226 82 277 57 22 126 96 65
Cuts at: 1687 1745 1779 1780 1907 1943 2006 2051 2080
Size: 58 34 1 127 36 63 45 29
Cuts at: 2080 2087 2104 2105 2632 2889 2928 2989 2990
Size: 7 17 1 527 257 39 61 1
Cuts at: 2990 3375 3573
Size: 385 198
Fragments arranged by size:
527 394 385 277 257 226 198 173
127 126 96 82 67 65 63 61
58 57 57 45 39 36 35 34
29 22 17 8 7 1 1 1
1 1
EcoRV GAT'ATC
Cuts at: 0 369 580 3573
Size: 369 211 2993
Fragments arranged by size:
2993 369 211
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2865 3573
Size: 2865 708
FspI TGC'GCA
Cuts at: 0 2653 3573
Size: 2653 920
HaeII r_GCGC'y
Cuts at: 0 1105 1795 2171 3016 3459 3466 3573
Size: 1105 690 376 845 443 7 107
Fragments arranged by size:
1105 845 690 443 376 107 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 421 453 1305 1337 3135 3167
Size: 13 32 376 32 852 32 1798 32
Cuts at: 3167 3573
Size: 406
Fragments arranged by size:
1798 852 406 376 32 32 32 32
13
HincII GTy'rAC
Cuts at: 0 201 3573
Size: 201 3372
KasI G'GCGC_C
Cuts at: 0 1791 3573
Size: 1791 1782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1742 3573
Size: 193 1549 1831
Fragments arranged by size:
1831 1549 193
MscI TGG'CCA
Cuts at: 0 2380 3573
Size: 2380 1193
MslI CAynn'nnrTG
Cuts at: 0 835 3573
Size: 835 2738
MspA1I CmG'CkG
Cuts at: 0 2788 3022 3070 3289 3420 3445 3501 3516
Size: 2788 234 48 219 131 25 56 15
Cuts at: 3516 3573
Size: 57
Fragments arranged by size:
2788 234 219 131 57 56 48 25
15
NaeI GCC'GGC
Cuts at: 0 2192 3383 3573
Size: 2192 1191 190
Fragments arranged by size:
2192 1191 190
NarI GG'CG_CC
Cuts at: 0 1792 3573
Size: 1792 1781
NcoI C'CATG_G
Cuts at: 0 590 1001 3573
Size: 590 411 2572
Fragments arranged by size:
2572 590 411
NgoMIV G'CCGG_C
Cuts at: 0 2190 3381 3573
Size: 2190 1191 192
Fragments arranged by size:
2190 1191 192
NheI G'CTAG_C
Cuts at: 0 541 3573
Size: 541 3032
NsiI A_TGCA'T
Cuts at: 0 939 3573
Size: 939 2634
NspI r_CATG'y
Cuts at: 0 3048 3490 3573
Size: 3048 442 83
Fragments arranged by size:
3048 442 83
PasI CC'CwG_GG
Cuts at: 0 61 456 1608 1776 2047 2724 2893 3102
Size: 61 395 1152 168 271 677 169 209
Cuts at: 3102 3573
Size: 471
Fragments arranged by size:
1152 677 471 395 271 209 169 168
61
PciI A'CATG_T
Cuts at: 0 3044 3573
Size: 3044 529
PflMI CCAn_nnn'nTGG
Cuts at: 0 841 976 1002 1557 3573
Size: 841 135 26 555 2016
Fragments arranged by size:
2016 841 555 135 26
PfoI T'CCnGG_A
Cuts at: 0 1536 3573
Size: 1536 2037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2810 2842 3573
Size: 2810 32 731
Fragments arranged by size:
2810 731 32
PpuMI rG'GwC_Cy
Cuts at: 0 700 1321 1745 2006 2051 2080 2632 2889
Size: 700 621 424 261 45 29 552 257
Cuts at: 2889 3375 3573
Size: 486 198
Fragments arranged by size:
700 621 552 486 424 261 257 198
45 29
PspOMI G'GGCC_C
Cuts at: 0 64 459 632 735 962 1044 1400 1525
Size: 64 395 173 103 227 82 356 125
Cuts at: 1525 1779 1906 2086 2104 2565 2989 3573
Size: 254 127 180 18 461 424 584
Fragments arranged by size:
584 461 424 395 356 254 227 180
173 127 125 103 82 64 18
PstI C_TGCA'G
Cuts at: 0 1149 2043 2942 3131 3425 3455 3573
Size: 1149 894 899 189 294 30 118
Fragments arranged by size:
1149 899 894 294 189 118 30
PvuII CAG'CTG
Cuts at: 0 2788 3022 3070 3289 3420 3445 3573
Size: 2788 234 48 219 131 25 128
Fragments arranged by size:
2788 234 219 131 128 48 25
SanDI GG'GwC_CC
Cuts at: 0 700 1321 2006 2889 3573
Size: 700 621 685 883 684
Fragments arranged by size:
883 700 685 684 621
SbfI CC_TGCA'GG
Cuts at: 0 2043 3573
Size: 2043 1530
SexAI A'CCwGG_T
Cuts at: 0 975 2396 3573
Size: 975 1421 1177
Fragments arranged by size:
1421 1177 975
SfcI C'TryA_G
Cuts at: 0 405 1145 1674 2039 2612 2938 3127 3421
Size: 405 740 529 365 573 326 189 294
Cuts at: 3421 3451 3537 3573
Size: 30 86 36
Fragments arranged by size:
740 573 529 405 365 326 294 189
86 36 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1694 2954 3573
Size: 1694 1260 619
Fragments arranged by size:
1694 1260 619
SfoI GGC'GCC
Cuts at: 0 1793 3573
Size: 1793 1780
SgrAI Cr'CCGG_yG
Cuts at: 0 1809 3573
Size: 1809 1764
SmaI CCC'GGG
Cuts at: 0 70 1940 1980 2741 2794 3573
Size: 70 1870 40 761 53 779
Fragments arranged by size:
1870 779 761 70 53 40
SmlI C'TyrA_G
Cuts at: 0 1739 1784 2839 3573
Size: 1739 45 1055 734
Fragments arranged by size:
1739 1055 734 45
SphI G_CATG'C
Cuts at: 0 3490 3573
Size: 3490 83
StuI AGG'CCT
Cuts at: 0 2114 3573
Size: 2114 1459
StyI C'CwwG_G
Cuts at: 0 361 590 704 1001 2255 3573
Size: 361 229 114 297 1254 1318
Fragments arranged by size:
1318 1254 361 297 229 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 494 585 662 780 829 1006 1433 1568
Size: 494 91 77 118 49 177 427 135
Cuts at: 1568 3573
Size: 2005
Fragments arranged by size:
2005 494 427 177 135 118 91 77
49
TatI w'GTAC_w
Cuts at: 0 2348 2771 3573
Size: 2348 423 802
Fragments arranged by size:
2348 802 423
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3148 3180 3573
Size: 3148 32 393
Fragments arranged by size:
3148 393 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 625 973 3573
Size: 625 348 2600
Fragments arranged by size:
2600 625 348
XmaI C'CCGG_G
Cuts at: 0 68 1938 1978 2739 2792 3573
Size: 68 1870 40 761 53 781
Fragments arranged by size:
1870 781 761 68 53 40
XmnI GAAnn'nnTTC
Cuts at: 0 3341 3573
Size: 3341 232
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApaLI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BciVI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsiWI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI FspI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SanDI SbfI SexAI SfcI SfiI SfoI SgrAI SmaI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AlfI ApoI AscI AseI
AsiSI BaeI BcgI BclI BsaBI BsiEI BsmBI BspEI
BspHI BstBI ClaI DraI DrdI EagI EcoICRI EcoRI
FseI FspAI HindIII HpaI KpnI MfeI MluI NdeI
NotI NruI PacI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI ScaI
SgfI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI ZraI