Restriction map for
pFN21AE4584
(Linear) (Six-base) MAPSORT of: KIEE4584.seq Check: 6895 from: 1 to: 2559
KIEE4584.orf
With 182 enzymes: SgfI *
March 22, 2012 18:26 ..
AatII G_ACGT'C
Cuts at: 0 557 2559
Size: 557 2002
Acc65I G'GTAC_C
Cuts at: 0 1649 2559
Size: 1649 910
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 679 1486 1753 2559
Size: 462 217 807 267 806
Fragments arranged by size:
807 806 462 267 217
AflIII A'CryG_T
Cuts at: 0 863 2559
Size: 863 1696
AhdI GACnn_n'nnGTC
Cuts at: 0 867 2559
Size: 867 1692
AleI CACnn'nnGTG
Cuts at: 0 2341 2559
Size: 2341 218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 274 2559
Size: 190 84 2285
Fragments arranged by size:
2285 190 84
AlwNI CAG_nnn'CTG
Cuts at: 0 714 1272 1941 2559
Size: 714 558 669 618
Fragments arranged by size:
714 669 618 558
ApaI G_GGCC'C
Cuts at: 0 907 1017 1116 1487 2559
Size: 907 110 99 371 1072
Fragments arranged by size:
1072 907 371 110 99
ApaLI G'TGCA_C
Cuts at: 0 1530 2334 2559
Size: 1530 804 225
Fragments arranged by size:
1530 804 225
ApoI r'AATT_y
Cuts at: 0 639 853 1099 1507 2510 2559
Size: 639 214 246 408 1003 49
Fragments arranged by size:
1003 639 408 246 214 49
AvaI C'yCGr_G
Cuts at: 0 900 2559
Size: 900 1659
BanI G'GyrC_C
Cuts at: 0 116 242 369 1649 1676 1807 1880 2559
Size: 116 126 127 1280 27 131 73 679
Fragments arranged by size:
1280 679 131 127 126 116 73 27
BanII G_rGCy'C
Cuts at: 0 85 500 907 1017 1116 1487 2300 2559
Size: 85 415 407 110 99 371 813 259
Fragments arranged by size:
813 415 407 371 259 110 99 85
BbeI G_GCGC'C
Cuts at: 0 246 1680 2559
Size: 246 1434 879
Fragments arranged by size:
1434 879 246
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 384 617 927 2531 2559
Size: 219 165 233 310 1604 28
Fragments arranged by size:
1604 310 233 219 165 28
BbvCI CC'TCA_GC
Cuts at: 0 999 2559
Size: 999 1560
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 537 571 1320 1354 2140 2174 2559
Size: 537 34 749 34 786 34 385
Fragments arranged by size:
786 749 537 385 34 34 34
BclI T'GATC_A
Cuts at: 0 566 2559
Size: 566 1993
BglI GCCn_nnn'nGGC
Cuts at: 0 316 2559
Size: 316 2243
BglII A'GATC_T
Cuts at: 0 662 2559
Size: 662 1897
BlpI GC'TnA_GC
Cuts at: 0 1850 2559
Size: 1850 709
Bme1580I G_kGCm'C
Cuts at: 0 121 374 472 907 1017 1116 1487 1534
Size: 121 253 98 435 110 99 371 47
Cuts at: 1534 2338 2559
Size: 804 221
Fragments arranged by size:
804 435 371 253 221 121 110 99
98 47
BmgBI CAC'GTC
Cuts at: 0 459 864 2559
Size: 459 405 1695
Fragments arranged by size:
1695 459 405
BmrI ACTGGGnnnn_n'
Cuts at: 0 1020 2559
Size: 1020 1539
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 834 2559
Size: 834 1725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 376 1342 1546 1774 1909 2122 2482
Size: 199 177 966 204 228 135 213 360
Cuts at: 2482 2559
Size: 77
Fragments arranged by size:
966 360 228 213 204 199 177 135
77
Bpu10I CC'TnA_GC
Cuts at: 0 673 999 2559
Size: 673 326 1560
Fragments arranged by size:
1560 673 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 1912 2559
Size: 1912 647
BsaAI yAC'GTr
Cuts at: 0 423 1041 1365 1965 2559
Size: 423 618 324 600 594
Fragments arranged by size:
618 600 594 423 324
BsaBI GATnn'nnATC
Cuts at: 0 1981 2559
Size: 1981 578
BsaHI Gr'CG_yC
Cuts at: 0 243 554 1677 2559
Size: 243 311 1123 882
Fragments arranged by size:
1123 882 311 243
BsaWI w'CCGG_w
Cuts at: 0 1652 2279 2559
Size: 1652 627 280
Fragments arranged by size:
1652 627 280
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2080 2110 2559
Size: 2080 30 449
Fragments arranged by size:
2080 449 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 175 757 988 1192 1486 1854 1930
Size: 28 147 582 231 204 294 368 76
Cuts at: 1930 2479 2488 2491 2494 2559
Size: 549 9 3 3 65
Fragments arranged by size:
582 549 368 294 231 204 147 76
65 28 9 3 3
BseYI C'CCAG_C
Cuts at: 0 890 1452 1578 2081 2526 2559
Size: 890 562 126 503 445 33
Fragments arranged by size:
890 562 503 445 126 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 234 358 2559
Size: 208 26 124 2201
Fragments arranged by size:
2201 208 124 26
BsiEI CG_ry'CG
Cuts at: 0 270 2294 2394 2559
Size: 270 2024 100 165
Fragments arranged by size:
2024 270 165 100
BsiHKAI G_wGCw'C
Cuts at: 0 169 500 1534 2300 2338 2559
Size: 169 331 1034 766 38 221
Fragments arranged by size:
1034 766 331 221 169 38
BsmI GAATG_Cn'
Cuts at: 0 1080 2559
Size: 1080 1479
BsmBI CGTCTCn'nnnn_
Cuts at: 0 688 2098 2559
Size: 688 1410 461
Fragments arranged by size:
1410 688 461
Bsp1286I G_dGCh'C
Cuts at: 0 85 121 169 374 472 500 907 1017
Size: 85 36 48 205 98 28 407 110
Cuts at: 1017 1116 1487 1534 2300 2338 2559
Size: 99 371 47 766 38 221
Fragments arranged by size:
766 407 371 221 205 110 99 98
85 48 47 38 36 28
BspHI T'CATG_A
Cuts at: 0 539 1070 1718 2559
Size: 539 531 648 841
Fragments arranged by size:
841 648 539 531
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2554 2559
Size: 2554 5
BsrBI CCG'CTC
Cuts at: 0 813 1878 2559
Size: 813 1065 681
Fragments arranged by size:
1065 813 681
BsrDI GCAATG_nn'
Cuts at: 0 2523 2559
Size: 2523 36
BsrFI r'CCGG_y
Cuts at: 0 1723 1777 1946 2559
Size: 1723 54 169 613
Fragments arranged by size:
1723 613 169 54
BsrGI T'GTAC_A
Cuts at: 0 2345 2559
Size: 2345 214
BssHII G'CGCG_C
Cuts at: 0 1411 2559
Size: 1411 1148
BssSI C'ACGA_G
Cuts at: 0 2203 2559
Size: 2203 356
BstAPI GCAn_nnn'nTGC
Cuts at: 0 195 2559
Size: 195 2364
BstXI CCAn_nnnn'nTGG
Cuts at: 0 67 441 2004 2559
Size: 67 374 1563 555
Fragments arranged by size:
1563 555 374 67
BstYI r'GATC_y
Cuts at: 0 662 1385 1601 2375 2559
Size: 662 723 216 774 184
Fragments arranged by size:
774 723 662 216 184
BtgI C'CryG_G
Cuts at: 0 98 702 821 963 1680 1766 1803 1857
Size: 98 604 119 142 717 86 37 54
Cuts at: 1857 1997 2559
Size: 140 562
Fragments arranged by size:
717 604 562 142 140 119 98 86
54 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1323 1454 1968 1977 2044 2559
Size: 1323 131 514 9 67 515
Fragments arranged by size:
1323 515 514 131 67 9
BtsI GCAGTG_nn'
Cuts at: 0 581 2559
Size: 581 1978
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1022 1057 1476 1511 2559
Size: 1022 35 419 35 1048
Fragments arranged by size:
1048 1022 419 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 409 2559
Size: 409 2150
EaeI y'GGCC_r
Cuts at: 0 95 308 317 461 527 705 725 921
Size: 95 213 9 144 66 178 20 196
Cuts at: 921 1019 2129 2291 2559
Size: 98 1110 162 268
Fragments arranged by size:
1110 268 213 196 178 162 144 98
95 66 20 9
EagI C'GGCC_G
Cuts at: 0 2291 2559
Size: 2291 268
EarI CTCTTCn'nnn_
Cuts at: 0 286 1729 2017 2071 2366 2559
Size: 286 1443 288 54 295 193
Fragments arranged by size:
1443 295 288 286 193 54
EcoICRI GAG'CTC
Cuts at: 0 498 2298 2559
Size: 498 1800 261
Fragments arranged by size:
1800 498 261
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 376 462 679 1342 1486 1546 1753
Size: 199 177 86 217 663 144 60 207
Cuts at: 1753 1774 1909 2122 2482 2559
Size: 21 135 213 360 77
Fragments arranged by size:
663 360 217 213 207 199 177 144
135 86 77 60 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 151 259 1420 1468 2559
Size: 10 141 108 1161 48 1091
Fragments arranged by size:
1161 1091 141 108 48 10
EcoO109I rG'GnC_Cy
Cuts at: 0 144 234 1014 1112 1113 1483 1614 2559
Size: 144 90 780 98 1 370 131 945
Fragments arranged by size:
945 780 370 144 131 98 90 1
EcoRI G'AATT_C
Cuts at: 0 1507 2559
Size: 1507 1052
HaeII r_GCGC'y
Cuts at: 0 175 246 1680 2428 2559
Size: 175 71 1434 748 131
Fragments arranged by size:
1434 748 175 131 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 853 885 2080 2112 2559
Size: 853 32 1195 32 447
Fragments arranged by size:
1195 853 447 32 32
HincII GTy'rAC
Cuts at: 0 1167 2559
Size: 1167 1392
KasI G'GCGC_C
Cuts at: 0 242 1676 2559
Size: 242 1434 883
Fragments arranged by size:
1434 883 242
KpnI G_GTAC'C
Cuts at: 0 1653 2559
Size: 1653 906
MscI TGG'CCA
Cuts at: 0 319 529 1021 2131 2559
Size: 319 210 492 1110 428
Fragments arranged by size:
1110 492 428 319 210
MslI CAynn'nnrTG
Cuts at: 0 211 574 1564 2131 2137 2269 2341 2559
Size: 211 363 990 567 6 132 72 218
Fragments arranged by size:
990 567 363 218 211 132 72 6
MspA1I CmG'CkG
Cuts at: 0 100 272 704 1025 1084 1805 2559
Size: 100 172 432 321 59 721 754
Fragments arranged by size:
754 721 432 321 172 100 59
NaeI GCC'GGC
Cuts at: 0 1779 2559
Size: 1779 780
NarI GG'CG_CC
Cuts at: 0 243 1677 2559
Size: 243 1434 882
Fragments arranged by size:
1434 882 243
NcoI C'CATG_G
Cuts at: 0 1766 2559
Size: 1766 793
NgoMIV G'CCGG_C
Cuts at: 0 1777 2559
Size: 1777 782
PasI CC'CwG_GG
Cuts at: 0 2174 2559
Size: 2174 385
PflMI CCAn_nnn'nTGG
Cuts at: 0 1977 2174 2559
Size: 1977 197 385
Fragments arranged by size:
1977 385 197
PfoI T'CCnGG_A
Cuts at: 0 140 353 830 2559
Size: 140 213 477 1729
Fragments arranged by size:
1729 477 213 140
PmlI CAC'GTG
Cuts at: 0 423 1365 1965 2559
Size: 423 942 600 594
Fragments arranged by size:
942 600 594 423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 751 783 2559
Size: 751 32 1776
Fragments arranged by size:
1776 751 32
PpuMI rG'GwC_Cy
Cuts at: 0 144 234 1614 2559
Size: 144 90 1380 945
Fragments arranged by size:
1380 945 144 90
PspOMI G'GGCC_C
Cuts at: 0 903 1013 1112 1483 2559
Size: 903 110 99 371 1076
Fragments arranged by size:
1076 903 371 110 99
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2338 2370 2559
Size: 2338 32 189
Fragments arranged by size:
2338 189 32
PstI C_TGCA'G
Cuts at: 0 263 277 2402 2559
Size: 263 14 2125 157
Fragments arranged by size:
2125 263 157 14
SacI G_AGCT'C
Cuts at: 0 500 2300 2559
Size: 500 1800 259
Fragments arranged by size:
1800 500 259
SacII CC_GC'GG
Cuts at: 0 101 705 1806 2559
Size: 101 604 1101 753
Fragments arranged by size:
1101 753 604 101
SapI GCTCTTCn'nnn_
Cuts at: 0 1729 2071 2559
Size: 1729 342 488
Fragments arranged by size:
1729 488 342
SbfI CC_TGCA'GG
Cuts at: 0 263 2559
Size: 263 2296
ScaI AGT'ACT
Cuts at: 0 64 2559
Size: 64 2495
SexAI A'CCwGG_T
Cuts at: 0 1544 2559
Size: 1544 1015
SfcI C'TryA_G
Cuts at: 0 259 273 2398 2559
Size: 259 14 2125 161
Fragments arranged by size:
2125 259 161 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 316 2559
Size: 316 2243
SfoI GGC'GCC
Cuts at: 0 244 1678 2559
Size: 244 1434 881
Fragments arranged by size:
1434 881 244
StuI AGG'CCT
Cuts at: 0 778 1759 2559
Size: 778 981 800
Fragments arranged by size:
981 800 778
StyI C'CwwG_G
Cuts at: 0 842 1766 1844 2559
Size: 842 924 78 715
Fragments arranged by size:
924 842 715 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 549 2073 2408 2547 2559
Size: 549 1524 335 139 12
Fragments arranged by size:
1524 549 335 139 12
TatI w'GTAC_w
Cuts at: 0 62 1568 2012 2345 2559
Size: 62 1506 444 333 214
Fragments arranged by size:
1506 444 333 214 62
Tth111I GACn'n_nGTC
Cuts at: 0 349 1351 1546 2559
Size: 349 1002 195 1013
Fragments arranged by size:
1013 1002 349 195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1413 1891 2271 2310 2559
Size: 1413 478 380 39 249
Fragments arranged by size:
1413 478 380 249 39
ZraI GAC'GTC
Cuts at: 0 555 2559
Size: 555 2004
Enzymes that do cut:
AatII Acc65I AcuI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII KasI KpnI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI
PfoI PmlI PpiI PpuMI PspOMI PsrI PstI SacI
SacII SapI SbfI ScaI SexAI SfcI SfiI SfoI
StuI StyI TaqII TatI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AloI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BmtI
BpuEI BsiWI BspEI BstBI BstEII BstZ17I Bsu36I ClaI
DraI DraIII EciI EcoRV FalI FseI FspI FspAI
HindIII HpaI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PshAI PsiI
PspXI PvuI PvuII RsrII SalI SanDI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI XhoI XmaI XmnI