Restriction map for
pFN21AE4582
(Linear) (Six-base) MAPSORT of: KIEE4582.seq Check: 7966 from: 1 to: 2451
KIEE4582.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1356 1734 2022 2451
Size: 1356 378 288 429
Fragments arranged by size:
1356 429 378 288
AccI GT'mk_AC
Cuts at: 0 1454 2451
Size: 1454 997
Acc65I G'GTAC_C
Cuts at: 0 1876 2451
Size: 1876 575
AclI AA'CG_TT
Cuts at: 0 2219 2451
Size: 2219 232
AleI CACnn'nnGTG
Cuts at: 0 538 1156 2451
Size: 538 618 1295
Fragments arranged by size:
1295 618 538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 64 2451
Size: 64 2387
AlwNI CAG_nnn'CTG
Cuts at: 0 612 688 1779 2037 2368 2395 2451
Size: 612 76 1091 258 331 27 56
Fragments arranged by size:
1091 612 331 258 76 56 27
ApaLI G'TGCA_C
Cuts at: 0 2332 2451
Size: 2332 119
ApoI r'AATT_y
Cuts at: 0 821 2451
Size: 821 1630
AvaI C'yCGr_G
Cuts at: 0 245 275 517 965 1040 1347 2438 2451
Size: 245 30 242 448 75 307 1091 13
Fragments arranged by size:
1091 448 307 245 242 75 30 13
BanI G'GyrC_C
Cuts at: 0 307 749 1045 1876 1914 2451
Size: 307 442 296 831 38 537
Fragments arranged by size:
831 537 442 307 296 38
BanII G_rGCy'C
Cuts at: 0 135 253 358 1723 2451
Size: 135 118 105 1365 728
Fragments arranged by size:
1365 728 135 118 105
BbsI GAAGACnn'nnnn_
Cuts at: 0 240 2127 2166 2451
Size: 240 1887 39 285
Fragments arranged by size:
1887 285 240 39
BbvCI CC'TCA_GC
Cuts at: 0 1487 2451
Size: 1487 964
BciVI GTATCCnnnnn_n'
Cuts at: 0 282 2451
Size: 282 2169
BclI T'GATC_A
Cuts at: 0 542 1799 2451
Size: 542 1257 652
Fragments arranged by size:
1257 652 542
BglII A'GATC_T
Cuts at: 0 725 2451
Size: 725 1726
BlpI GC'TnA_GC
Cuts at: 0 352 1087 1722 2451
Size: 352 735 635 729
Fragments arranged by size:
735 729 635 352
Bme1580I G_kGCm'C
Cuts at: 0 535 754 2336 2451
Size: 535 219 1582 115
Fragments arranged by size:
1582 535 219 115
BmgBI CAC'GTC
Cuts at: 0 2016 2451
Size: 2016 435
BmrI ACTGGGnnnn_n'
Cuts at: 0 189 544 946 2101 2451
Size: 189 355 402 1155 350
Fragments arranged by size:
1155 402 355 350 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 186 320 1291 2417 2451
Size: 56 130 134 971 1126 34
Fragments arranged by size:
1126 971 134 130 56 34
Bpu10I CC'TnA_GC
Cuts at: 0 1487 2451
Size: 1487 964
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 2451
Size: 514 1937
BsaI GGTCTCn'nnnn_
Cuts at: 0 941 1791 1885 2413 2451
Size: 941 850 94 528 38
Fragments arranged by size:
941 850 528 94 38
BsaAI yAC'GTr
Cuts at: 0 540 771 2451
Size: 540 231 1680
Fragments arranged by size:
1680 540 231
BsaBI GATnn'nnATC
Cuts at: 0 730 1798 2451
Size: 730 1068 653
Fragments arranged by size:
1068 730 653
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 54 84 685 715 938 968 1189 1219
Size: 54 30 601 30 223 30 221 30
Cuts at: 1219 2087 2117 2451
Size: 868 30 334
Fragments arranged by size:
868 601 334 223 221 54 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 125 128 439 442 1570 2451
Size: 125 3 311 3 1128 881
Fragments arranged by size:
1128 881 311 125 3 3
BseYI C'CCAG_C
Cuts at: 0 688 1130 2361 2451
Size: 688 442 1231 90
Fragments arranged by size:
1231 688 442 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1819 2451
Size: 1819 632
BsiHKAI G_wGCw'C
Cuts at: 0 358 386 1277 2336 2451
Size: 358 28 891 1059 115
Fragments arranged by size:
1059 891 358 115 28
Bsp1286I G_dGCh'C
Cuts at: 0 135 253 358 386 535 754 1277 1723
Size: 135 118 105 28 149 219 523 446
Cuts at: 1723 2336 2451
Size: 613 115
Fragments arranged by size:
613 523 446 219 149 135 118 115
105 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 626 1356 1734 2022 2451
Size: 626 730 378 288 429
Fragments arranged by size:
730 626 429 378 288
BsrBI CCG'CTC
Cuts at: 0 855 1659 2027 2451
Size: 855 804 368 424
Fragments arranged by size:
855 804 424 368
BsrDI GCAATG_nn'
Cuts at: 0 726 2244 2297 2451
Size: 726 1518 53 154
Fragments arranged by size:
1518 726 154 53
BsrFI r'CCGG_y
Cuts at: 0 263 917 1964 2451
Size: 263 654 1047 487
Fragments arranged by size:
1047 654 487 263
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1409 2451
Size: 1409 1042
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1061 1746 1947 2430 2451
Size: 1061 685 201 483 21
Fragments arranged by size:
1061 685 483 201 21
BstYI r'GATC_y
Cuts at: 0 725 1079 1338 1776 2210 2451
Size: 725 354 259 438 434 241
Fragments arranged by size:
725 438 434 354 259 241
BtgI C'CryG_G
Cuts at: 0 426 882 942 980 1235 1686 2451
Size: 426 456 60 38 255 451 765
Fragments arranged by size:
765 456 451 426 255 60 38
BtsI GCAGTG_nn'
Cuts at: 0 573 1404 2108 2251 2451
Size: 573 831 704 143 200
Fragments arranged by size:
831 704 573 200 143
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 582 617 2451
Size: 582 35 1834
Fragments arranged by size:
1834 582 35
DraIII CAC_nnn'GTG
Cuts at: 0 888 2451
Size: 888 1563
DrdI GACnn_nn'nnGTC
Cuts at: 0 1006 2451
Size: 1006 1445
EaeI y'GGCC_r
Cuts at: 0 870 1122 1178 1736 2039 2109 2451
Size: 870 252 56 558 303 70 342
Fragments arranged by size:
870 558 342 303 252 70 56
EarI CTCTTCn'nnn_
Cuts at: 0 742 1324 2451
Size: 742 582 1127
Fragments arranged by size:
1127 742 582
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1197 2451
Size: 1197 1254
EcoICRI GAG'CTC
Cuts at: 0 356 2451
Size: 356 2095
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 186 320 1291 2417 2451
Size: 56 130 134 971 1126 34
Fragments arranged by size:
1126 971 134 130 56 34
EcoO109I rG'GnC_Cy
Cuts at: 0 184 1922 2284 2451
Size: 184 1738 362 167
Fragments arranged by size:
1738 362 184 167
FspI TGC'GCA
Cuts at: 0 2065 2451
Size: 2065 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 938 970 1097 1129 1323 1330
Size: 85 32 821 32 127 32 194 7
Cuts at: 1330 1355 1362 2408 2440 2451
Size: 25 7 1046 32 11
Fragments arranged by size:
1046 821 194 127 85 32 32 32
32 25 11 7 7
KpnI G_GTAC'C
Cuts at: 0 1880 2451
Size: 1880 571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1625 2451
Size: 1625 826
MscI TGG'CCA
Cuts at: 0 1124 1180 1738 2041 2111 2451
Size: 1124 56 558 303 70 340
Fragments arranged by size:
1124 558 340 303 70 56
MslI CAynn'nnrTG
Cuts at: 0 538 550 1059 1156 1744 1945 2347 2428
Size: 538 12 509 97 588 201 402 81
Cuts at: 2428 2451
Size: 23
Fragments arranged by size:
588 538 509 402 201 97 81 23
12
MspA1I CmG'CkG
Cuts at: 0 284 298 428 1518 1726 2451
Size: 284 14 130 1090 208 725
Fragments arranged by size:
1090 725 284 208 130 14
NaeI GCC'GGC
Cuts at: 0 265 2451
Size: 265 2186
NcoI C'CATG_G
Cuts at: 0 980 1235 2451
Size: 980 255 1216
Fragments arranged by size:
1216 980 255
NdeI CA'TA_TG
Cuts at: 0 443 933 2451
Size: 443 490 1518
Fragments arranged by size:
1518 490 443
NgoMIV G'CCGG_C
Cuts at: 0 263 2451
Size: 263 2188
NspI r_CATG'y
Cuts at: 0 327 1608 2451
Size: 327 1281 843
Fragments arranged by size:
1281 843 327
PasI CC'CwG_GG
Cuts at: 0 2368 2451
Size: 2368 83
PflMI CCAn_nnn'nTGG
Cuts at: 0 1977 2368 2451
Size: 1977 391 83
Fragments arranged by size:
1977 391 83
PfoI T'CCnGG_A
Cuts at: 0 460 911 2451
Size: 460 451 1540
Fragments arranged by size:
1540 460 451
PmlI CAC'GTG
Cuts at: 0 540 2451
Size: 540 1911
PpuMI rG'GwC_Cy
Cuts at: 0 184 2451
Size: 184 2267
PvuII CAG'CTG
Cuts at: 0 298 2451
Size: 298 2153
RsrII CG'GwC_CG
Cuts at: 0 1929 2451
Size: 1929 522
SacI G_AGCT'C
Cuts at: 0 358 2451
Size: 358 2093
SacII CC_GC'GG
Cuts at: 0 429 2451
Size: 429 2022
SmaI CCC'GGG
Cuts at: 0 247 277 967 2451
Size: 247 30 690 1484
Fragments arranged by size:
1484 690 247 30
SmlI C'TyrA_G
Cuts at: 0 493 2438 2451
Size: 493 1945 13
Fragments arranged by size:
1945 493 13
SphI G_CATG'C
Cuts at: 0 327 2451
Size: 327 2124
StyI C'CwwG_G
Cuts at: 0 980 1235 2451
Size: 980 255 1216
Fragments arranged by size:
1216 980 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 850 1053 1349 1652 2451
Size: 850 203 296 303 799
Fragments arranged by size:
850 799 303 296 203
TatI w'GTAC_w
Cuts at: 0 1508 2124 2451
Size: 1508 616 327
Fragments arranged by size:
1508 616 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 54 86 2087 2119 2451
Size: 54 32 2001 32 332
Fragments arranged by size:
2001 332 54 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 952 2451
Size: 952 1499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1902 2451
Size: 1902 549
XhoI C'TCGA_G
Cuts at: 0 2438 2451
Size: 2438 13
XmaI C'CCGG_G
Cuts at: 0 245 275 965 2451
Size: 245 30 690 1486
Fragments arranged by size:
1486 690 245 30
Enzymes that do cut:
AarI AccI Acc65I AclI AleI AlfI AlwNI ApaLI
ApoI AvaI BanI BanII BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI
BtgI BtsI CspCI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I FspI Hin4I KpnI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI
PflMI PfoI PmlI PpuMI PvuII RsrII SacI SacII
SmaI SmlI SphI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AcuI AfeI AflII AflIII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BfrBI BglI BmtI BplI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI EagI
EcoNI EcoRI EcoRV FalI FseI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI NarI NheI NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI XbaI XmnI
ZraI