Restriction map for
pFN21AE4581
(Linear) (Six-base) MAPSORT of: KIEE4581.seq Check: 2802 from: 1 to: 2415
KIEE4581.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1227 2415
Size: 1227 1188
AatII G_ACGT'C
Cuts at: 0 1787 2415
Size: 1787 628
AccI GT'mk_AC
Cuts at: 0 1487 2415
Size: 1487 928
Acc65I G'GTAC_C
Cuts at: 0 2366 2415
Size: 2366 49
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2143 2415
Size: 2143 272
AfeI AGC'GCT
Cuts at: 0 376 2415
Size: 376 2039
AflIII A'CryG_T
Cuts at: 0 1043 2174 2415
Size: 1043 1131 241
Fragments arranged by size:
1131 1043 241
AhdI GACnn_n'nnGTC
Cuts at: 0 1962 2415
Size: 1962 453
AleI CACnn'nnGTG
Cuts at: 0 1900 2415
Size: 1900 515
AlwNI CAG_nnn'CTG
Cuts at: 0 877 966 1320 2031 2415
Size: 877 89 354 711 384
Fragments arranged by size:
877 711 384 354 89
ApaI G_GGCC'C
Cuts at: 0 2289 2415
Size: 2289 126
ApaLI G'TGCA_C
Cuts at: 0 2196 2415
Size: 2196 219
ApoI r'AATT_y
Cuts at: 0 1014 2415
Size: 1014 1401
BamHI G'GATC_C
Cuts at: 0 401 2415
Size: 401 2014
BanI G'GyrC_C
Cuts at: 0 60 2111 2241 2300 2366 2415
Size: 60 2051 130 59 66 49
Fragments arranged by size:
2051 130 66 60 59 49
BanII G_rGCy'C
Cuts at: 0 571 909 1333 1520 2289 2399 2415
Size: 571 338 424 187 769 110 16
Fragments arranged by size:
769 571 424 338 187 110 16
BbeI G_GCGC'C
Cuts at: 0 64 2115 2415
Size: 64 2051 300
Fragments arranged by size:
2051 300 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 1122 1530 1779 2415
Size: 1122 408 249 636
Fragments arranged by size:
1122 636 408 249
BclI T'GATC_A
Cuts at: 0 683 2415
Size: 683 1732
BglI GCCn_nnn'nGGC
Cuts at: 0 1270 2019 2415
Size: 1270 749 396
Fragments arranged by size:
1270 749 396
BglII A'GATC_T
Cuts at: 0 821 2415
Size: 821 1594
BlpI GC'TnA_GC
Cuts at: 0 1951 2415
Size: 1951 464
Bme1580I G_kGCm'C
Cuts at: 0 1835 2200 2246 2289 2415
Size: 1835 365 46 43 126
Fragments arranged by size:
1835 365 126 46 43
BmgBI CAC'GTC
Cuts at: 0 1947 2415
Size: 1947 468
BmrI ACTGGGnnnn_n'
Cuts at: 0 1854 2415
Size: 1854 561
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1395 2415
Size: 1395 1020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 993 2008 2415
Size: 343 650 1015 407
Fragments arranged by size:
1015 650 407 343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 445 655 2415
Size: 259 186 210 1760
Fragments arranged by size:
1760 259 210 186
BsaAI yAC'GTr
Cuts at: 0 660 2175 2415
Size: 660 1515 240
Fragments arranged by size:
1515 660 240
BsaHI Gr'CG_yC
Cuts at: 0 61 100 1784 1934 1963 2112 2138 2254
Size: 61 39 1684 150 29 149 26 116
Cuts at: 2254 2415
Size: 161
Fragments arranged by size:
1684 161 150 149 116 61 39 29
26
BsaWI w'CCGG_w
Cuts at: 0 356 694 2415
Size: 356 338 1721
Fragments arranged by size:
1721 356 338
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 850 880 896 926 931 961 2415
Size: 850 30 16 30 5 30 1454
Fragments arranged by size:
1454 850 30 30 30 16 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 340 343 952 2407 2415
Size: 67 273 3 609 1455 8
Fragments arranged by size:
1455 609 273 67 8 3
BseYI C'CCAG_C
Cuts at: 0 118 377 1661 1665 2009 2045 2415
Size: 118 259 1284 4 344 36 370
Fragments arranged by size:
1284 370 344 259 118 36 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1382 1825 2415
Size: 1382 443 590
Fragments arranged by size:
1382 590 443
BsiEI CG_ry'CG
Cuts at: 0 36 884 2415
Size: 36 848 1531
Fragments arranged by size:
1531 848 36
BsiHKAI G_wGCw'C
Cuts at: 0 563 1760 1897 2200 2399 2415
Size: 563 1197 137 303 199 16
Fragments arranged by size:
1197 563 303 199 137 16
BsmBI CGTCTCn'nnnn_
Cuts at: 0 15 870 2129 2415
Size: 15 855 1259 286
Fragments arranged by size:
1259 855 286 15
Bsp1286I G_dGCh'C
Cuts at: 0 563 571 909 1333 1520 1760 1835 1897
Size: 563 8 338 424 187 240 75 62
Cuts at: 1897 2200 2246 2289 2399 2415
Size: 303 46 43 110 16
Fragments arranged by size:
563 424 338 303 240 187 110 75
62 46 43 16 8
BspEI T'CCGG_A
Cuts at: 0 356 2415
Size: 356 2059
BspHI T'CATG_A
Cuts at: 0 1706 1760 2219 2415
Size: 1706 54 459 196
Fragments arranged by size:
1706 459 196 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1227 1915 2415
Size: 1227 688 500
Fragments arranged by size:
1227 688 500
BsrBI CCG'CTC
Cuts at: 0 333 2085 2190 2415
Size: 333 1752 105 225
Fragments arranged by size:
1752 333 225 105
BsrFI r'CCGG_y
Cuts at: 0 594 1223 1574 2415
Size: 594 629 351 841
Fragments arranged by size:
841 629 594 351
BssHII G'CGCG_C
Cuts at: 0 133 2415
Size: 133 2282
BssSI C'ACGA_G
Cuts at: 0 415 562 1117 2415
Size: 415 147 555 1298
Fragments arranged by size:
1298 555 415 147
BstAPI GCAn_nnn'nTGC
Cuts at: 0 877 1830 2415
Size: 877 953 585
Fragments arranged by size:
953 877 585
BstBI TT'CG_AA
Cuts at: 0 1206 2415
Size: 1206 1209
BstEII G'GTnAC_C
Cuts at: 0 1857 2343 2415
Size: 1857 486 72
Fragments arranged by size:
1857 486 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1146 1752 1776 2415
Size: 1146 606 24 639
Fragments arranged by size:
1146 639 606 24
BstYI r'GATC_y
Cuts at: 0 245 401 650 779 821 977 1121 1586
Size: 245 156 249 129 42 156 144 465
Cuts at: 1586 2415
Size: 829
Fragments arranged by size:
829 465 249 245 156 156 144 129
42
Bsu36I CC'TnA_GG
Cuts at: 0 1912 2116 2415
Size: 1912 204 299
Fragments arranged by size:
1912 299 204
BtgI C'CryG_G
Cuts at: 0 710 728 911 1035 1139 1382 1985 2201
Size: 710 18 183 124 104 243 603 216
Cuts at: 2201 2263 2415
Size: 62 152
Fragments arranged by size:
710 603 243 216 183 152 124 104
62 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1119 2415
Size: 1119 1296
BtsI GCAGTG_nn'
Cuts at: 0 1175 1874 2415
Size: 1175 699 541
Fragments arranged by size:
1175 699 541
DraIII CAC_nnn'GTG
Cuts at: 0 663 2073 2307 2415
Size: 663 1410 234 108
Fragments arranged by size:
1410 663 234 108
EaeI y'GGCC_r
Cuts at: 0 2 33 725 881 1259 1271 2415
Size: 2 31 692 156 378 12 1144
Fragments arranged by size:
1144 692 378 156 31 12 2
EagI C'GGCC_G
Cuts at: 0 33 881 2415
Size: 33 848 1534
Fragments arranged by size:
1534 848 33
EarI CTCTTCn'nnn_
Cuts at: 0 1408 1435 2415
Size: 1408 27 980
Fragments arranged by size:
1408 980 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 351 524 1639 2415
Size: 351 173 1115 776
Fragments arranged by size:
1115 776 351 173
EcoICRI GAG'CTC
Cuts at: 0 2397 2415
Size: 2397 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 993 2008 2143 2415
Size: 343 650 1015 135 272
Fragments arranged by size:
1015 650 343 272 135
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 1911 2415
Size: 325 1586 504
Fragments arranged by size:
1586 504 325
EcoO109I rG'GnC_Cy
Cuts at: 0 720 921 1050 1797 1851 2028 2286 2355
Size: 720 201 129 747 54 177 258 69
Cuts at: 2355 2415
Size: 60
Fragments arranged by size:
747 720 258 201 177 129 69 60
54
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 876 2403 2415
Size: 876 1527 12
Fragments arranged by size:
1527 876 12
HaeII r_GCGC'y
Cuts at: 0 64 378 2115 2415
Size: 64 314 1737 300
Fragments arranged by size:
1737 314 300 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 850 882 931 963 1948 1980 2415
Size: 850 32 49 32 985 32 435
Fragments arranged by size:
985 850 435 49 32 32 32
HincII GTy'rAC
Cuts at: 0 797 2415
Size: 797 1618
KasI G'GCGC_C
Cuts at: 0 60 2111 2415
Size: 60 2051 304
Fragments arranged by size:
2051 304 60
KpnI G_GTAC'C
Cuts at: 0 2370 2415
Size: 2370 45
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 2114 2415
Size: 147 1967 301
Fragments arranged by size:
1967 301 147
MscI TGG'CCA
Cuts at: 0 4 2415
Size: 4 2411
MslI CAynn'nnrTG
Cuts at: 0 439 1138 1144 1708 1753 1900 2000 2173
Size: 439 699 6 564 45 147 100 173
Cuts at: 2173 2415
Size: 242
Fragments arranged by size:
699 564 439 242 173 147 100 45
6
MspA1I CmG'CkG
Cuts at: 0 66 105 315 611 895 1347 1665 1821
Size: 66 39 210 296 284 452 318 156
Cuts at: 1821 2109 2415
Size: 288 306
Fragments arranged by size:
452 318 306 296 288 284 210 156
66 39
NaeI GCC'GGC
Cuts at: 0 1576 2415
Size: 1576 839
NarI GG'CG_CC
Cuts at: 0 61 2112 2415
Size: 61 2051 303
Fragments arranged by size:
2051 303 61
NcoI C'CATG_G
Cuts at: 0 1139 1382 2201 2415
Size: 1139 243 819 214
Fragments arranged by size:
1139 819 243 214
NdeI CA'TA_TG
Cuts at: 0 173 2415
Size: 173 2242
NgoMIV G'CCGG_C
Cuts at: 0 1574 2415
Size: 1574 841
NspI r_CATG'y
Cuts at: 0 1047 1812 1999 2415
Size: 1047 765 187 416
Fragments arranged by size:
1047 765 416 187
PasI CC'CwG_GG
Cuts at: 0 2289 2415
Size: 2289 126
PciI A'CATG_T
Cuts at: 0 1043 2415
Size: 1043 1372
PfoI T'CCnGG_A
Cuts at: 0 320 2415
Size: 320 2095
PmlI CAC'GTG
Cuts at: 0 660 2175 2415
Size: 660 1515 240
Fragments arranged by size:
1515 660 240
PpuMI rG'GwC_Cy
Cuts at: 0 921 1050 2355 2415
Size: 921 129 1305 60
Fragments arranged by size:
1305 921 129 60
PspOMI G'GGCC_C
Cuts at: 0 2285 2415
Size: 2285 130
PstI C_TGCA'G
Cuts at: 0 1238 1325 1352 1826 2415
Size: 1238 87 27 474 589
Fragments arranged by size:
1238 589 474 87 27
PvuII CAG'CTG
Cuts at: 0 315 1665 1821 2415
Size: 315 1350 156 594
Fragments arranged by size:
1350 594 315 156
SacI G_AGCT'C
Cuts at: 0 2399 2415
Size: 2399 16
SapI GCTCTTCn'nnn_
Cuts at: 0 1408 2415
Size: 1408 1007
SbfI CC_TGCA'GG
Cuts at: 0 1238 1325 2415
Size: 1238 87 1090
Fragments arranged by size:
1238 1090 87
ScaI AGT'ACT
Cuts at: 0 832 2415
Size: 832 1583
SexAI A'CCwGG_T
Cuts at: 0 2303 2415
Size: 2303 112
SfcI C'TryA_G
Cuts at: 0 1234 1321 1348 1822 2415
Size: 1234 87 27 474 593
Fragments arranged by size:
1234 593 474 87 27
SfoI GGC'GCC
Cuts at: 0 62 2113 2415
Size: 62 2051 302
Fragments arranged by size:
2051 302 62
SmlI C'TyrA_G
Cuts at: 0 274 460 670 2415
Size: 274 186 210 1745
Fragments arranged by size:
1745 274 210 186
SphI G_CATG'C
Cuts at: 0 1999 2415
Size: 1999 416
StuI AGG'CCT
Cuts at: 0 1067 2415
Size: 1067 1348
StyI C'CwwG_G
Cuts at: 0 239 1139 1382 2201 2415
Size: 239 900 243 819 214
Fragments arranged by size:
900 819 243 239 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 904 2023 2172 2415
Size: 904 1119 149 243
Fragments arranged by size:
1119 904 243 149
TatI w'GTAC_w
Cuts at: 0 464 830 1373 2415
Size: 464 366 543 1042
Fragments arranged by size:
1042 543 464 366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 218 250 896 928 2415
Size: 218 32 646 32 1487
Fragments arranged by size:
1487 646 218 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 862 2415
Size: 862 1553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1146 2097 2370 2415
Size: 1146 951 273 45
Fragments arranged by size:
1146 951 273 45
XmnI GAAnn'nnTTC
Cuts at: 0 961 2128 2415
Size: 961 1167 287
Fragments arranged by size:
1167 961 287
ZraI GAC'GTC
Cuts at: 0 1785 2415
Size: 1785 630
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlwNI ApaI ApaLI ApoI BamHI BanI BanII
BbeI BbsI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SapI SbfI ScaI SexAI SfcI SfoI SmlI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AlfI AloI AscI AseI AsiSI
AvaI AvrII BaeI BbvCI BcgI BciVI BfrBI BmtI
Bpu10I BsaI BsaBI BsiWI BsmI BsrDI BsrGI BstZ17I
ClaI CspCI DraI DrdI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI MfeI MluI NheI NotI NruI
NsiI PacI PflMI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI XmaI