Restriction map for
pFN21AE4579
(Linear) (Six-base) MAPSORT of: KIEE4579.seq Check: 1588 from: 1 to: 2355
KIEE4579.orf
With 182 enzymes: SgfI *
March 22, 2012 18:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 64 336 1251 2355
Size: 64 272 915 1104
Fragments arranged by size:
1104 915 272 64
AatII G_ACGT'C
Cuts at: 0 1292 2355
Size: 1292 1063
AccI GT'mk_AC
Cuts at: 0 1859 2355
Size: 1859 496
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1107 1159 1350 1585 1651 2355
Size: 259 848 52 191 235 66 704
Fragments arranged by size:
848 704 259 235 191 66 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 239 2355
Size: 239 2116
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 563 595 2355
Size: 563 32 1760
Fragments arranged by size:
1760 563 32
AlwNI CAG_nnn'CTG
Cuts at: 0 402 731 2355
Size: 402 329 1624
Fragments arranged by size:
1624 402 329
ApaI G_GGCC'C
Cuts at: 0 2221 2355
Size: 2221 134
ApaLI G'TGCA_C
Cuts at: 0 166 2355
Size: 166 2189
ApoI r'AATT_y
Cuts at: 0 1952 2355
Size: 1952 403
AvaI C'yCGr_G
Cuts at: 0 483 1751 2355
Size: 483 1268 604
Fragments arranged by size:
1268 604 483
BamHI G'GATC_C
Cuts at: 0 1692 2187 2355
Size: 1692 495 168
Fragments arranged by size:
1692 495 168
BanI G'GyrC_C
Cuts at: 0 461 1669 2355
Size: 461 1208 686
Fragments arranged by size:
1208 686 461
BanII G_rGCy'C
Cuts at: 0 83 484 1752 2221 2355
Size: 83 401 1268 469 134
Fragments arranged by size:
1268 469 401 134 83
BbeI G_GCGC'C
Cuts at: 0 1673 2355
Size: 1673 682
BbsI GAAGACnn'nnnn_
Cuts at: 0 889 1299 1573 2019 2355
Size: 889 410 274 446 336
Fragments arranged by size:
889 446 410 336 274
BbvCI CC'TCA_GC
Cuts at: 0 1215 2355
Size: 1215 1140
BciVI GTATCCnnnnn_n'
Cuts at: 0 781 2355
Size: 781 1574
BglI GCCn_nnn'nGGC
Cuts at: 0 306 1649 2355
Size: 306 1343 706
Fragments arranged by size:
1343 706 306
BglII A'GATC_T
Cuts at: 0 1430 2348 2355
Size: 1430 918 7
Fragments arranged by size:
1430 918 7
Bme1580I G_kGCm'C
Cuts at: 0 145 170 284 311 719 1186 2221 2355
Size: 145 25 114 27 408 467 1035 134
Fragments arranged by size:
1035 467 408 145 134 114 27 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 539 1176 1640 2355
Size: 539 637 464 715
Fragments arranged by size:
715 637 539 464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 343 1428 1537 1570 1594 2278 2292
Size: 229 114 1085 109 33 24 684 14
Cuts at: 2292 2355
Size: 63
Fragments arranged by size:
1085 684 229 114 109 63 33 24
14
Bpu10I CC'TnA_GC
Cuts at: 0 406 1215 2355
Size: 406 809 1140
Fragments arranged by size:
1140 809 406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1978 2355
Size: 1978 377
BsaAI yAC'GTr
Cuts at: 0 1280 2355
Size: 1280 1075
BsaHI Gr'CG_yC
Cuts at: 0 1289 1670 2355
Size: 1289 381 685
Fragments arranged by size:
1289 685 381
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 1015 1045 1510 1540 2355
Size: 343 30 642 30 465 30 815
Fragments arranged by size:
815 642 465 343 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1081 1387 2355
Size: 1081 306 968
Fragments arranged by size:
1081 968 306
BseYI C'CCAG_C
Cuts at: 0 158 304 364 860 1736 2355
Size: 158 146 60 496 876 619
Fragments arranged by size:
876 619 496 158 146 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 1176 1358 1874 1897 2355
Size: 716 460 182 516 23 458
Fragments arranged by size:
716 516 460 458 182 23
BsiHKAI G_wGCw'C
Cuts at: 0 170 2355
Size: 170 2185
BsmI GAATG_Cn'
Cuts at: 0 258 1425 2355
Size: 258 1167 930
Fragments arranged by size:
1167 930 258
Bsp1286I G_dGCh'C
Cuts at: 0 83 145 170 284 311 484 719 1186
Size: 83 62 25 114 27 173 235 467
Cuts at: 1186 1752 2221 2355
Size: 566 469 134
Fragments arranged by size:
566 469 467 235 173 134 114 83
62 27 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 64 336 757 1251 1919 2261 2355
Size: 64 272 421 494 668 342 94
Fragments arranged by size:
668 494 421 342 272 94 64
BsrDI GCAATG_nn'
Cuts at: 0 463 528 587 1825 2355
Size: 463 65 59 1238 530
Fragments arranged by size:
1238 530 463 65 59
BsrFI r'CCGG_y
Cuts at: 0 315 2355
Size: 315 2040
BssSI C'ACGA_G
Cuts at: 0 1414 2355
Size: 1414 941
BstAPI GCAn_nnn'nTGC
Cuts at: 0 21 402 1190 2355
Size: 21 381 788 1165
Fragments arranged by size:
1165 788 381 21
BstEII G'GTnAC_C
Cuts at: 0 1347 2355
Size: 1347 1008
BstXI CCAn_nnnn'nTGG
Cuts at: 0 304 907 955 2355
Size: 304 603 48 1400
Fragments arranged by size:
1400 603 304 48
BstYI r'GATC_y
Cuts at: 0 1430 1692 2187 2348 2355
Size: 1430 262 495 161 7
Fragments arranged by size:
1430 495 262 161 7
Bsu36I CC'TnA_GG
Cuts at: 0 608 1958 2355
Size: 608 1350 397
Fragments arranged by size:
1350 608 397
BtgI C'CryG_G
Cuts at: 0 681 737 1609 2355
Size: 681 56 872 746
Fragments arranged by size:
872 746 681 56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 705 747 1746 2355
Size: 705 42 999 609
Fragments arranged by size:
999 705 609 42
BtsI GCAGTG_nn'
Cuts at: 0 818 1592 2355
Size: 818 774 763
Fragments arranged by size:
818 774 763
EaeI y'GGCC_r
Cuts at: 0 135 2355
Size: 135 2220
EarI CTCTTCn'nnn_
Cuts at: 0 88 194 2355
Size: 88 106 2161
Fragments arranged by size:
2161 106 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 235 2355
Size: 235 2120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 259 343 1107 1159 1350 1428 1537
Size: 229 30 84 764 52 191 78 109
Cuts at: 1537 1570 1585 1594 1651 2278 2292 2355
Size: 33 15 9 57 627 14 63
Fragments arranged by size:
764 627 229 191 109 84 78 63
57 52 33 30 15 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1177 2355
Size: 1177 1178
EcoO109I rG'GnC_Cy
Cuts at: 0 1170 1211 1521 1757 2217 2218 2355
Size: 1170 41 310 236 460 1 137
Fragments arranged by size:
1170 460 310 236 137 41 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 936 2355
Size: 936 1419
FseI GG_CCGG'CC
Cuts at: 0 319 2355
Size: 319 2036
HaeII r_GCGC'y
Cuts at: 0 1228 1673 2297 2355
Size: 1228 445 624 58
Fragments arranged by size:
1228 624 445 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 613 645 927 959 2146 2178 2355
Size: 613 32 282 32 1187 32 177
Fragments arranged by size:
1187 613 282 177 32 32 32
KasI G'GCGC_C
Cuts at: 0 1669 2355
Size: 1669 686
MscI TGG'CCA
Cuts at: 0 137 2355
Size: 137 2218
MslI CAynn'nnrTG
Cuts at: 0 32 499 659 967 2355
Size: 32 467 160 308 1388
Fragments arranged by size:
1388 467 308 160 32
MspA1I CmG'CkG
Cuts at: 0 854 860 1219 1237 1827 2355
Size: 854 6 359 18 590 528
Fragments arranged by size:
854 590 528 359 18 6
NaeI GCC'GGC
Cuts at: 0 317 2355
Size: 317 2038
NarI GG'CG_CC
Cuts at: 0 1670 2355
Size: 1670 685
NcoI C'CATG_G
Cuts at: 0 681 2355
Size: 681 1674
NgoMIV G'CCGG_C
Cuts at: 0 315 2355
Size: 315 2040
NspI r_CATG'y
Cuts at: 0 1080 1582 2049 2355
Size: 1080 502 467 306
Fragments arranged by size:
1080 502 467 306
PasI CC'CwG_GG
Cuts at: 0 42 98 2355
Size: 42 56 2257
Fragments arranged by size:
2257 56 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 1166 1433 2355
Size: 1166 267 922
Fragments arranged by size:
1166 922 267
PfoI T'CCnGG_A
Cuts at: 0 581 2355
Size: 581 1774
PmlI CAC'GTG
Cuts at: 0 1280 2355
Size: 1280 1075
PpuMI rG'GwC_Cy
Cuts at: 0 1170 1211 1521 1757 2355
Size: 1170 41 310 236 598
Fragments arranged by size:
1170 598 310 236 41
PshAI GACnn'nnGTC
Cuts at: 0 1441 2355
Size: 1441 914
PspOMI G'GGCC_C
Cuts at: 0 2217 2355
Size: 2217 138
PstI C_TGCA'G
Cuts at: 0 859 1224 1262 2355
Size: 859 365 38 1093
Fragments arranged by size:
1093 859 365 38
PvuII CAG'CTG
Cuts at: 0 854 860 1219 1237 1827 2355
Size: 854 6 359 18 590 528
Fragments arranged by size:
854 590 528 359 18 6
SapI GCTCTTCn'nnn_
Cuts at: 0 88 2355
Size: 88 2267
SfcI C'TryA_G
Cuts at: 0 600 849 855 1220 1258 1761 2355
Size: 600 249 6 365 38 503 594
Fragments arranged by size:
600 594 503 365 249 38 6
SfoI GGC'GCC
Cuts at: 0 1671 2355
Size: 1671 684
SmaI CCC'GGG
Cuts at: 0 485 2355
Size: 485 1870
SmlI C'TyrA_G
Cuts at: 0 1993 2355
Size: 1993 362
SphI G_CATG'C
Cuts at: 0 1080 2355
Size: 1080 1275
SrfI GCCC'GGGC
Cuts at: 0 485 2355
Size: 485 1870
StyI C'CwwG_G
Cuts at: 0 227 681 2138 2355
Size: 227 454 1457 217
Fragments arranged by size:
1457 454 227 217
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 609 1622 2355
Size: 609 1013 733
Fragments arranged by size:
1013 733 609
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1510 1542 2355
Size: 1510 32 813
Fragments arranged by size:
1510 813 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1056 2355
Size: 1056 1299
XmaI C'CCGG_G
Cuts at: 0 483 2355
Size: 483 1872
XmnI GAAnn'nnTTC
Cuts at: 0 1636 2355
Size: 1636 719
ZraI GAC'GTC
Cuts at: 0 1290 2355
Size: 1290 1065
Enzymes that do cut:
AarI AatII AccI AcuI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BglI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PstI PvuII SapI SfcI SfoI SmaI SmlI SphI
SrfI StyI TaqII TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI
AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI
BlpI BmgBI BmtI BplI BsaI BsaBI BsaWI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstBI
BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SspI StuI SwaI
TatI Tth111I XbaI XhoI