Restriction map for
pFN21AE4575
(Linear) (Six-base) MAPSORT of: KIEE4575.seq Check: 6751 from: 1 to: 2274
KIEE4575.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AccI GT'mk_AC
Cuts at: 0 353 1768 2274
Size: 353 1415 506
Fragments arranged by size:
1415 506 353
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 1351 2274
Size: 696 655 923
Fragments arranged by size:
923 696 655
AflIII A'CryG_T
Cuts at: 0 1796 2274
Size: 1796 478
AleI CACnn'nnGTG
Cuts at: 0 770 1582 2274
Size: 770 812 692
Fragments arranged by size:
812 770 692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1678 1760 2274
Size: 1678 82 514
Fragments arranged by size:
1678 514 82
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 463 495 2274
Size: 463 32 1779
Fragments arranged by size:
1779 463 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1665 1920 2274
Size: 1665 255 354
Fragments arranged by size:
1665 354 255
ApaI G_GGCC'C
Cuts at: 0 83 1948 2274
Size: 83 1865 326
Fragments arranged by size:
1865 326 83
ApaLI G'TGCA_C
Cuts at: 0 763 1207 2274
Size: 763 444 1067
Fragments arranged by size:
1067 763 444
ApoI r'AATT_y
Cuts at: 0 867 1623 2274
Size: 867 756 651
Fragments arranged by size:
867 756 651
BanII G_rGCy'C
Cuts at: 0 83 254 1388 1948 2274
Size: 83 171 1134 560 326
Fragments arranged by size:
1134 560 326 171 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 1581 1643 2274
Size: 1581 62 631
Fragments arranged by size:
1581 631 62
BbvCI CC'TCA_GC
Cuts at: 0 1010 2274
Size: 1010 1264
BglI GCCn_nnn'nGGC
Cuts at: 0 1259 2274
Size: 1259 1015
BglII A'GATC_T
Cuts at: 0 617 1391 2274
Size: 617 774 883
Fragments arranged by size:
883 774 617
BlpI GC'TnA_GC
Cuts at: 0 189 253 2274
Size: 189 64 2021
Fragments arranged by size:
2021 189 64
Bme1580I G_kGCm'C
Cuts at: 0 83 378 587 767 1211 1948 2274
Size: 83 295 209 180 444 737 326
Fragments arranged by size:
737 444 326 295 209 180 83
BmrI ACTGGGnnnn_n'
Cuts at: 0 388 675 777 1010 1276 2274
Size: 388 287 102 233 266 998
Fragments arranged by size:
998 388 287 266 233 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1135 1554 1852 1981 2158 2274
Size: 1135 419 298 129 177 116
Fragments arranged by size:
1135 419 298 177 129 116
Bpu10I CC'TnA_GC
Cuts at: 0 1010 1351 1949 2274
Size: 1010 341 598 325
Fragments arranged by size:
1010 598 341 325
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 982 2193 2274
Size: 807 175 1211 81
Fragments arranged by size:
1211 807 175 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 1416 2169 2274
Size: 1416 753 105
Fragments arranged by size:
1416 753 105
BsaAI yAC'GTr
Cuts at: 0 582 1230 2274
Size: 582 648 1044
Fragments arranged by size:
1044 648 582
BsaHI Gr'CG_yC
Cuts at: 0 1983 2274
Size: 1983 291
BsaWI w'CCGG_w
Cuts at: 0 266 404 2274
Size: 266 138 1870
Fragments arranged by size:
1870 266 138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 463 493 1201 1231 1979 2009 2274
Size: 463 30 708 30 748 30 265
Fragments arranged by size:
748 708 463 265 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 754 1053 2274
Size: 754 299 1221
Fragments arranged by size:
1221 754 299
BseYI C'CCAG_C
Cuts at: 0 34 390 872 1083 2274
Size: 34 356 482 211 1191
Fragments arranged by size:
1191 482 356 211 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1486 1597 2274
Size: 1486 111 677
Fragments arranged by size:
1486 677 111
BsiHKAI G_wGCw'C
Cuts at: 0 608 767 1211 2274
Size: 608 159 444 1063
Fragments arranged by size:
1063 608 444 159
BsmI GAATG_Cn'
Cuts at: 0 564 579 2274
Size: 564 15 1695
Fragments arranged by size:
1695 564 15
Bsp1286I G_dGCh'C
Cuts at: 0 83 254 378 587 608 767 1211 1388
Size: 83 171 124 209 21 159 444 177
Cuts at: 1388 1948 2274
Size: 560 326
Fragments arranged by size:
560 444 326 209 177 171 159 124
83 21
BsrBI CCG'CTC
Cuts at: 0 246 1217 2274
Size: 246 971 1057
Fragments arranged by size:
1057 971 246
BsrDI GCAATG_nn'
Cuts at: 0 635 1592 2274
Size: 635 957 682
Fragments arranged by size:
957 682 635
BsrFI r'CCGG_y
Cuts at: 0 2012 2274
Size: 2012 262
BssSI C'ACGA_G
Cuts at: 0 1451 2274
Size: 1451 823
BstBI TT'CG_AA
Cuts at: 0 2109 2274
Size: 2109 165
BstEII G'GTnAC_C
Cuts at: 0 153 1478 2274
Size: 153 1325 796
Fragments arranged by size:
1325 796 153
BstXI CCAn_nnnn'nTGG
Cuts at: 0 338 2274
Size: 338 1936
BstYI r'GATC_y
Cuts at: 0 617 826 1391 2274
Size: 617 209 565 883
Fragments arranged by size:
883 617 565 209
BstZ17I GTA'TAC
Cuts at: 0 1769 2274
Size: 1769 505
BtgI C'CryG_G
Cuts at: 0 609 683 2274
Size: 609 74 1591
Fragments arranged by size:
1591 609 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1320 2274
Size: 1320 954
BtsI GCAGTG_nn'
Cuts at: 0 325 608 858 2274
Size: 325 283 250 1416
Fragments arranged by size:
1416 325 283 250
DrdI GACnn_nn'nnGTC
Cuts at: 0 132 2274
Size: 132 2142
EaeI y'GGCC_r
Cuts at: 0 612 911 1056 1251 2274
Size: 612 299 145 195 1023
Fragments arranged by size:
1023 612 299 195 145
EarI CTCTTCn'nnn_
Cuts at: 0 715 931 1721 2274
Size: 715 216 790 553
Fragments arranged by size:
790 715 553 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 159 2274
Size: 159 2115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 1135 1351 1554 1852 1981 2158 2274
Size: 696 439 216 203 298 129 177 116
Fragments arranged by size:
696 439 298 216 203 177 129 116
EcoO109I rG'GnC_Cy
Cuts at: 0 79 1894 1944 1945 2274
Size: 79 1815 50 1 329
Fragments arranged by size:
1815 329 79 50 1
EcoRI G'AATT_C
Cuts at: 0 867 2274
Size: 867 1407
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 2274
Size: 448 32 1794
Fragments arranged by size:
1794 448 32
HincII GTy'rAC
Cuts at: 0 1812 2274
Size: 1812 462
HindIII A'AGCT_T
Cuts at: 0 186 2274
Size: 186 2088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1904 2172 2274
Size: 1904 268 102
Fragments arranged by size:
1904 268 102
MscI TGG'CCA
Cuts at: 0 614 913 1058 2274
Size: 614 299 145 1216
Fragments arranged by size:
1216 614 299 145
MslI CAynn'nnrTG
Cuts at: 0 361 770 889 1204 1582 2274
Size: 361 409 119 315 378 692
Fragments arranged by size:
692 409 378 361 315 119
MspA1I CmG'CkG
Cuts at: 0 796 1083 1662 2145 2274
Size: 796 287 579 483 129
Fragments arranged by size:
796 579 483 287 129
NaeI GCC'GGC
Cuts at: 0 2014 2274
Size: 2014 260
NcoI C'CATG_G
Cuts at: 0 683 2274
Size: 683 1591
NgoMIV G'CCGG_C
Cuts at: 0 2012 2274
Size: 2012 262
NspI r_CATG'y
Cuts at: 0 1329 1800 2274
Size: 1329 471 474
Fragments arranged by size:
1329 474 471
PasI CC'CwG_GG
Cuts at: 0 1152 1282 2274
Size: 1152 130 992
Fragments arranged by size:
1152 992 130
PciI A'CATG_T
Cuts at: 0 1796 2274
Size: 1796 478
PflMI CCAn_nnn'nTGG
Cuts at: 0 1083 2274
Size: 1083 1191
PfoI T'CCnGG_A
Cuts at: 0 452 1112 1997 2274
Size: 452 660 885 277
Fragments arranged by size:
885 660 452 277
PmlI CAC'GTG
Cuts at: 0 582 1230 2274
Size: 582 648 1044
Fragments arranged by size:
1044 648 582
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 463 495 2274
Size: 463 32 1779
Fragments arranged by size:
1779 463 32
PspOMI G'GGCC_C
Cuts at: 0 79 1944 2274
Size: 79 1865 330
Fragments arranged by size:
1865 330 79
PstI C_TGCA'G
Cuts at: 0 1670 1916 2274
Size: 1670 246 358
Fragments arranged by size:
1670 358 246
PvuII CAG'CTG
Cuts at: 0 796 1083 1662 2145 2274
Size: 796 287 579 483 129
Fragments arranged by size:
796 579 483 287 129
SapI GCTCTTCn'nnn_
Cuts at: 0 715 2274
Size: 715 1559
SfcI C'TryA_G
Cuts at: 0 1666 1817 1912 2274
Size: 1666 151 95 362
Fragments arranged by size:
1666 362 151 95
SgrAI Cr'CCGG_yG
Cuts at: 0 2012 2274
Size: 2012 262
SmlI C'TyrA_G
Cuts at: 0 822 997 2172 2274
Size: 822 175 1175 102
Fragments arranged by size:
1175 822 175 102
SspI AAT'ATT
Cuts at: 0 691 2274
Size: 691 1583
StyI C'CwwG_G
Cuts at: 0 683 2274
Size: 683 1591
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1165 1491 2274
Size: 1165 326 783
Fragments arranged by size:
1165 783 326
TatI w'GTAC_w
Cuts at: 0 48 629 2274
Size: 48 581 1645
Fragments arranged by size:
1645 581 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 399 431 1201 1233 2274
Size: 399 32 770 32 1041
Fragments arranged by size:
1041 770 399 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 2274
Size: 366 1908
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI ApoI BanII BbsI BbvCI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BsrBI BsrDI BsrFI BssSI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI DrdI EaeI EarI EciI
Eco57MI EcoO109I EcoRI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmlI PpiI PspOMI PstI PvuII SapI
SfcI SgrAI SmlI SspI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BcgI BciVI BclI BfrBI BmgBI BmtI BplI
BsaBI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrGI
BssHII BstAPI Bsu36I ClaI CspCI DraI DraIII EagI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PmeI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI