Restriction map for
pFN21AE4572
(Linear) (Six-base) MAPSORT of: KIEE4572.seq Check: 3923 from: 1 to: 2223
KIEE4572.orf
With 182 enzymes: SgfI *
March 22, 2012 18:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 553 1941 2223
Size: 553 1388 282
Fragments arranged by size:
1388 553 282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 1721 2044 2115 2223
Size: 265 1456 323 71 108
Fragments arranged by size:
1456 323 265 108 71
AflIII A'CryG_T
Cuts at: 0 494 1722 2223
Size: 494 1228 501
Fragments arranged by size:
1228 501 494
AleI CACnn'nnGTG
Cuts at: 0 1725 2223
Size: 1725 498
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 148 2223
Size: 148 2075
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1492 1524 2223
Size: 1492 32 699
Fragments arranged by size:
1492 699 32
AlwNI CAG_nnn'CTG
Cuts at: 0 39 150 1293 2223
Size: 39 111 1143 930
Fragments arranged by size:
1143 930 111 39
ApaI G_GGCC'C
Cuts at: 0 1564 2223
Size: 1564 659
ApoI r'AATT_y
Cuts at: 0 650 700 1153 1816 2223
Size: 650 50 453 663 407
Fragments arranged by size:
663 650 453 407 50
AseI AT'TA_AT
Cuts at: 0 395 1319 2223
Size: 395 924 904
Fragments arranged by size:
924 904 395
AvaI C'yCGr_G
Cuts at: 0 1933 2223
Size: 1933 290
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1467 1500 2223
Size: 1467 33 723
Fragments arranged by size:
1467 723 33
BamHI G'GATC_C
Cuts at: 0 538 722 746 2223
Size: 538 184 24 1477
Fragments arranged by size:
1477 538 184 24
BanI G'GyrC_C
Cuts at: 0 331 2073 2143 2156 2223
Size: 331 1742 70 13 67
Fragments arranged by size:
1742 331 70 67 13
BanII G_rGCy'C
Cuts at: 0 423 971 1043 1564 1934 2223
Size: 423 548 72 521 370 289
Fragments arranged by size:
548 521 423 370 289 72
BbeI G_GCGC'C
Cuts at: 0 2147 2223
Size: 2147 76
BbvCI CC'TCA_GC
Cuts at: 0 157 1223 1903 2223
Size: 157 1066 680 320
Fragments arranged by size:
1066 680 320 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 992 1026 2223
Size: 992 34 1197
Fragments arranged by size:
1197 992 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1495 2223
Size: 1495 728
BclI T'GATC_A
Cuts at: 0 630 2223
Size: 630 1593
BglII A'GATC_T
Cuts at: 0 1388 2223
Size: 1388 835
BlpI GC'TnA_GC
Cuts at: 0 965 1928 2223
Size: 965 963 295
Fragments arranged by size:
965 963 295
Bme1580I G_kGCm'C
Cuts at: 0 1564 2078 2223
Size: 1564 514 145
Fragments arranged by size:
1564 514 145
BmrI ACTGGGnnnn_n'
Cuts at: 0 940 1457 2223
Size: 940 517 766
Fragments arranged by size:
940 766 517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 1284 1428 2223
Size: 242 1042 144 795
Fragments arranged by size:
1042 795 242 144
Bpu10I CC'TnA_GC
Cuts at: 0 157 1223 1903 2223
Size: 157 1066 680 320
Fragments arranged by size:
1066 680 320 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 407 955 2223
Size: 406 1 548 1268
Fragments arranged by size:
1268 548 406 1
BsaAI yAC'GTr
Cuts at: 0 1470 1823 2223
Size: 1470 353 400
Fragments arranged by size:
1470 400 353
BsaHI Gr'CG_yC
Cuts at: 0 2144 2223
Size: 2144 79
BsaWI w'CCGG_w
Cuts at: 0 334 704 2223
Size: 334 370 1519
Fragments arranged by size:
1519 370 334
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1282 1312 2223
Size: 1282 30 911
Fragments arranged by size:
1282 911 30
BseYI C'CCAG_C
Cuts at: 0 39 370 1563 1685 2223
Size: 39 331 1193 122 538
Fragments arranged by size:
1193 538 331 122 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 2159 2223
Size: 73 2086 64
Fragments arranged by size:
2086 73 64
BsiHKAI G_wGCw'C
Cuts at: 0 423 971 1043 2223
Size: 423 548 72 1180
Fragments arranged by size:
1180 548 423 72
Bsp1286I G_dGCh'C
Cuts at: 0 423 971 1043 1564 1934 2078 2223
Size: 423 548 72 521 370 144 145
Fragments arranged by size:
548 521 423 370 145 144 72
BspHI T'CATG_A
Cuts at: 0 227 980 2223
Size: 227 753 1243
Fragments arranged by size:
1243 753 227
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 553 1511 1941 2047 2223
Size: 553 958 430 106 176
Fragments arranged by size:
958 553 430 176 106
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1171 2216 2223
Size: 1171 1045 7
Fragments arranged by size:
1171 1045 7
BstEII G'GTnAC_C
Cuts at: 0 2034 2116 2223
Size: 2034 82 107
Fragments arranged by size:
2034 107 82
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1727 2223
Size: 1727 496
BstYI r'GATC_y
Cuts at: 0 146 507 538 722 746 1388 2223
Size: 146 361 31 184 24 642 835
Fragments arranged by size:
835 642 361 184 146 31 24
BtgI C'CryG_G
Cuts at: 0 16 1479 1878 2223
Size: 16 1463 399 345
Fragments arranged by size:
1463 399 345 16
BtsI GCAGTG_nn'
Cuts at: 0 1775 2223
Size: 1775 448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1873 1908 2223
Size: 1873 35 315
Fragments arranged by size:
1873 315 35
DraI TTT'AAA
Cuts at: 0 1784 2223
Size: 1784 439
DraIII CAC_nnn'GTG
Cuts at: 0 17 2106 2223
Size: 17 2089 117
Fragments arranged by size:
2089 117 17
EaeI y'GGCC_r
Cuts at: 0 1717 1894 2057 2223
Size: 1717 177 163 166
Fragments arranged by size:
1717 177 166 163
EarI CTCTTCn'nnn_
Cuts at: 0 13 2134 2223
Size: 13 2121 89
Fragments arranged by size:
2121 89 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 661 2223
Size: 661 1562
EcoICRI GAG'CTC
Cuts at: 0 421 969 1041 2223
Size: 421 548 72 1182
Fragments arranged by size:
1182 548 421 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 265 1284 1428 1721 2044 2115 2223
Size: 242 23 1019 144 293 323 71 108
Fragments arranged by size:
1019 323 293 242 144 108 71 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 568 832 2005 2223
Size: 568 264 1173 218
Fragments arranged by size:
1173 568 264 218
EcoO109I rG'GnC_Cy
Cuts at: 0 1500 1560 2223
Size: 1500 60 663
Fragments arranged by size:
1500 663 60
EcoRI G'AATT_C
Cuts at: 0 700 2223
Size: 700 1523
EcoRV GAT'ATC
Cuts at: 0 408 1308 2223
Size: 408 900 915
Fragments arranged by size:
915 900 408
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 60 1410 2223
Size: 60 1350 813
Fragments arranged by size:
1350 813 60
HaeII r_GCGC'y
Cuts at: 0 1910 2147 2223
Size: 1910 237 76
Fragments arranged by size:
1910 237 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 707 739 1666 1698 2223
Size: 707 32 927 32 525
Fragments arranged by size:
927 707 525 32 32
HincII GTy'rAC
Cuts at: 0 1737 2223
Size: 1737 486
HindIII A'AGCT_T
Cuts at: 0 1921 2223
Size: 1921 302
KasI G'GCGC_C
Cuts at: 0 2143 2223
Size: 2143 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 1518 2223
Size: 278 1240 705
Fragments arranged by size:
1240 705 278
MscI TGG'CCA
Cuts at: 0 1896 2059 2223
Size: 1896 163 164
Fragments arranged by size:
1896 164 163
MslI CAynn'nnrTG
Cuts at: 0 1319 1414 1420 1725 2071 2223
Size: 1319 95 6 305 346 152
Fragments arranged by size:
1319 346 305 152 95 6
MspA1I CmG'CkG
Cuts at: 0 364 2135 2223
Size: 364 1771 88
Fragments arranged by size:
1771 364 88
NarI GG'CG_CC
Cuts at: 0 2144 2223
Size: 2144 79
NcoI C'CATG_G
Cuts at: 0 1479 1878 2223
Size: 1479 399 345
Fragments arranged by size:
1479 399 345
NdeI CA'TA_TG
Cuts at: 0 1812 2223
Size: 1812 411
NspI r_CATG'y
Cuts at: 0 498 1119 1429 1726 1809 2022 2223
Size: 498 621 310 297 83 213 201
Fragments arranged by size:
621 498 310 297 213 201 83
PciI A'CATG_T
Cuts at: 0 494 1722 2223
Size: 494 1228 501
Fragments arranged by size:
1228 501 494
PflMI CCAn_nnn'nTGG
Cuts at: 0 220 2223
Size: 220 2003
PfoI T'CCnGG_A
Cuts at: 0 149 2223
Size: 149 2074
PpuMI rG'GwC_Cy
Cuts at: 0 1500 2223
Size: 1500 723
PspOMI G'GGCC_C
Cuts at: 0 1560 2223
Size: 1560 663
PstI C_TGCA'G
Cuts at: 0 1508 2223
Size: 1508 715
PvuII CAG'CTG
Cuts at: 0 364 2135 2223
Size: 364 1771 88
Fragments arranged by size:
1771 364 88
SacI G_AGCT'C
Cuts at: 0 423 971 1043 2223
Size: 423 548 72 1180
Fragments arranged by size:
1180 548 423 72
SapI GCTCTTCn'nnn_
Cuts at: 0 2134 2223
Size: 2134 89
SfcI C'TryA_G
Cuts at: 0 359 675 1019 1504 1837 1912 2223
Size: 359 316 344 485 333 75 311
Fragments arranged by size:
485 359 344 333 316 311 75
SfoI GGC'GCC
Cuts at: 0 2145 2223
Size: 2145 78
SmaI CCC'GGG
Cuts at: 0 1935 2223
Size: 1935 288
SmlI C'TyrA_G
Cuts at: 0 385 422 970 2223
Size: 385 37 548 1253
Fragments arranged by size:
1253 548 385 37
SphI G_CATG'C
Cuts at: 0 1119 1429 2223
Size: 1119 310 794
Fragments arranged by size:
1119 794 310
SrfI GCCC'GGGC
Cuts at: 0 1935 2223
Size: 1935 288
SspI AAT'ATT
Cuts at: 0 669 1458 2223
Size: 669 789 765
Fragments arranged by size:
789 765 669
StyI C'CwwG_G
Cuts at: 0 598 833 1198 1479 1878 2060 2223
Size: 598 235 365 281 399 182 163
Fragments arranged by size:
598 399 365 281 235 182 163
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1638 2223
Size: 1638 585
TatI w'GTAC_w
Cuts at: 0 923 2027 2223
Size: 923 1104 196
Fragments arranged by size:
1104 923 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1282 1314 2223
Size: 1282 32 909
Fragments arranged by size:
1282 909 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2067 2223
Size: 2067 156
XmaI C'CCGG_G
Cuts at: 0 1933 2223
Size: 1933 290
XmnI GAAnn'nnTTC
Cuts at: 0 893 2223
Size: 893 1330
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AloI AlwNI ApaI
ApoI AseI AvaI BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BstAPI BstEII BstXI
BstYI BtgI BtsI CspCI DraI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NarI NcoI NdeI NspI PciI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SapI SfcI SfoI
SmaI SmlI SphI SrfI SspI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
ApaLI AscI AsiSI AvrII BbsI BfrBI BglI BmgBI
BmtI BplI BsaI BsaBI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I BtgZI ClaI DrdI EagI FseI
FspI FspAI HpaI KpnI MfeI MluI NaeI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI StuI SwaI Tth111I XbaI XhoI ZraI