Restriction map for
pFN21AE4571
(Linear) (Six-base) MAPSORT of: KIEE4571.seq Check: 4081 from: 1 to: 2211
KIEE4571.orf
With 182 enzymes: SgfI *
March 22, 2012 18:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1326 2211
Size: 1326 885
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 263 721 1081 1858 1903 2211
Size: 133 130 458 360 777 45 308
Fragments arranged by size:
777 458 360 308 133 130 45
AfeI AGC'GCT
Cuts at: 0 1858 2211
Size: 1858 353
AflIII A'CryG_T
Cuts at: 0 1217 2211
Size: 1217 994
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 248 2211
Size: 248 1963
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 362 394 436 468 1477 1509 2211
Size: 362 32 42 32 1009 32 702
Fragments arranged by size:
1009 702 362 42 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 831 1059 1881 2058 2080 2211
Size: 831 228 822 177 22 131
Fragments arranged by size:
831 822 228 177 131 22
ApaI G_GGCC'C
Cuts at: 0 1322 1917 2185 2211
Size: 1322 595 268 26
Fragments arranged by size:
1322 595 268 26
ApoI r'AATT_y
Cuts at: 0 712 979 2211
Size: 712 267 1232
Fragments arranged by size:
1232 712 267
AvaI C'yCGr_G
Cuts at: 0 740 971 1802 1954 2125 2211
Size: 740 231 831 152 171 86
Fragments arranged by size:
831 740 231 171 152 86
AvrII C'CTAG_G
Cuts at: 0 381 2211
Size: 381 1830
BamHI G'GATC_C
Cuts at: 0 899 2211
Size: 899 1312
BanI G'GyrC_C
Cuts at: 0 1790 2211
Size: 1790 421
BanII G_rGCy'C
Cuts at: 0 865 889 1322 1917 2185 2211
Size: 865 24 433 595 268 26
Fragments arranged by size:
865 595 433 268 26 24
BbeI G_GCGC'C
Cuts at: 0 1794 2211
Size: 1794 417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1362 1396 1912 1946 2211
Size: 1362 34 516 34 265
Fragments arranged by size:
1362 516 265 34 34
BclI T'GATC_A
Cuts at: 0 1427 2211
Size: 1427 784
BglI GCCn_nnn'nGGC
Cuts at: 0 547 2039 2211
Size: 547 1492 172
Fragments arranged by size:
1492 547 172
BglII A'GATC_T
Cuts at: 0 1577 2211
Size: 1577 634
BlpI GC'TnA_GC
Cuts at: 0 187 398 2211
Size: 187 211 1813
Fragments arranged by size:
1813 211 187
Bme1580I G_kGCm'C
Cuts at: 0 1322 1917 2185 2211
Size: 1322 595 268 26
Fragments arranged by size:
1322 595 268 26
BmgBI CAC'GTC
Cuts at: 0 1611 2049 2211
Size: 1611 438 162
Fragments arranged by size:
1611 438 162
BmrI ACTGGGnnnn_n'
Cuts at: 0 6 496 831 2180 2211
Size: 6 490 335 1349 31
Fragments arranged by size:
1349 490 335 31 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 748 805 1300 1494 1594 1681 1759
Size: 481 267 57 495 194 100 87 78
Cuts at: 1759 2211
Size: 452
Fragments arranged by size:
495 481 452 267 194 100 87 78
57
BsaI GGTCTCn'nnnn_
Cuts at: 0 1471 1549 2211
Size: 1471 78 662
Fragments arranged by size:
1471 662 78
BsaAI yAC'GTr
Cuts at: 0 1620 2211
Size: 1620 591
BsaHI Gr'CG_yC
Cuts at: 0 1791 2071 2211
Size: 1791 280 140
Fragments arranged by size:
1791 280 140
BsaWI w'CCGG_w
Cuts at: 0 1829 2211
Size: 1829 382
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 1105 1135 1752 1782 2211
Size: 436 30 639 30 617 30 429
Fragments arranged by size:
639 617 436 429 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 271 274 989 1297 2149 2211
Size: 271 3 715 308 852 62
Fragments arranged by size:
852 715 308 271 62 3
BseYI C'CCAG_C
Cuts at: 0 1224 1356 2211
Size: 1224 132 855
Fragments arranged by size:
1224 855 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1330 1354 2071 2211
Size: 208 1122 24 717 140
Fragments arranged by size:
1122 717 208 140 24
BsiEI CG_ry'CG
Cuts at: 0 1818 2211
Size: 1818 393
BsmI GAATG_Cn'
Cuts at: 0 1402 2211
Size: 1402 809
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1432 2211
Size: 1432 779
Bsp1286I G_dGCh'C
Cuts at: 0 865 889 1322 1917 2185 2211
Size: 865 24 433 595 268 26
Fragments arranged by size:
865 595 433 268 26 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 445 1326 2211
Size: 445 881 885
Fragments arranged by size:
885 881 445
BsrBI CCG'CTC
Cuts at: 0 1032 1472 2211
Size: 1032 440 739
Fragments arranged by size:
1032 739 440
BsrFI r'CCGG_y
Cuts at: 0 1162 2211
Size: 1162 1049
BsrGI T'GTAC_A
Cuts at: 0 1301 2211
Size: 1301 910
BssSI C'ACGA_G
Cuts at: 0 1348 2211
Size: 1348 863
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1353 2211
Size: 1353 858
BstEII G'GTnAC_C
Cuts at: 0 735 2211
Size: 735 1476
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1369 2211
Size: 1369 842
BstYI r'GATC_y
Cuts at: 0 899 1577 1826 2138 2211
Size: 899 678 249 312 73
Fragments arranged by size:
899 678 312 249 73
Bsu36I CC'TnA_GG
Cuts at: 0 161 1676 1972 2211
Size: 161 1515 296 239
Fragments arranged by size:
1515 296 239 161
BtgI C'CryG_G
Cuts at: 0 287 530 1362 2211
Size: 287 243 832 849
Fragments arranged by size:
849 832 287 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1488 2211
Size: 1488 723
BtsI GCAGTG_nn'
Cuts at: 0 1374 2211
Size: 1374 837
EaeI y'GGCC_r
Cuts at: 0 1901 2153 2202 2211
Size: 1901 252 49 9
Fragments arranged by size:
1901 252 49 9
EarI CTCTTCn'nnn_
Cuts at: 0 202 604 697 1384 1514 2211
Size: 202 402 93 687 130 697
Fragments arranged by size:
697 687 402 202 130 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 58 1798 2211
Size: 19 39 1740 413
Fragments arranged by size:
1740 413 39 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 263 481 721 748 805 1081 1300
Size: 133 130 218 240 27 57 276 219
Cuts at: 1300 1494 1594 1681 1759 1858 1903 2211
Size: 194 100 87 78 99 45 308
Fragments arranged by size:
308 276 240 219 218 194 133 130
100 99 87 78 57 45 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 253 766 1312 1921 2211
Size: 253 513 546 609 290
Fragments arranged by size:
609 546 513 290 253
EcoO109I rG'GnC_Cy
Cuts at: 0 165 210 423 913 1296 1318 1319 1914
Size: 165 45 213 490 383 22 1 595
Cuts at: 1914 2181 2182 2193 2211
Size: 267 1 11 18
Fragments arranged by size:
595 490 383 267 213 165 45 22
18 11 1 1
EcoRI G'AATT_C
Cuts at: 0 712 2211
Size: 712 1499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1290 2211
Size: 1290 921
HaeII r_GCGC'y
Cuts at: 0 93 1794 1860 2211
Size: 93 1701 66 351
Fragments arranged by size:
1701 351 93 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1449 1481 1527 1559 2182 2211
Size: 1105 32 312 32 46 32 623 29
Fragments arranged by size:
1105 623 312 46 32 32 32 29
HindIII A'AGCT_T
Cuts at: 0 1267 2211
Size: 1267 944
KasI G'GCGC_C
Cuts at: 0 1790 2211
Size: 1790 421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 2111 2211
Size: 326 1785 100
Fragments arranged by size:
1785 326 100
MscI TGG'CCA
Cuts at: 0 2155 2204 2211
Size: 2155 49 7
Fragments arranged by size:
2155 49 7
MslI CAynn'nnrTG
Cuts at: 0 37 230 1367 2211
Size: 37 193 1137 844
Fragments arranged by size:
1137 844 193 37
MspA1I CmG'CkG
Cuts at: 0 222 289 783 1056 1260 2077 2151 2211
Size: 222 67 494 273 204 817 74 60
Fragments arranged by size:
817 494 273 222 204 74 67 60
NaeI GCC'GGC
Cuts at: 0 1164 2211
Size: 1164 1047
NarI GG'CG_CC
Cuts at: 0 1791 2211
Size: 1791 420
NcoI C'CATG_G
Cuts at: 0 530 1362 2211
Size: 530 832 849
Fragments arranged by size:
849 832 530
NdeI CA'TA_TG
Cuts at: 0 1703 2211
Size: 1703 508
NgoMIV G'CCGG_C
Cuts at: 0 1162 2211
Size: 1162 1049
NspI r_CATG'y
Cuts at: 0 235 2211
Size: 235 1976
PasI CC'CwG_GG
Cuts at: 0 858 2211
Size: 858 1353
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 2119 2211
Size: 771 1348 92
Fragments arranged by size:
1348 771 92
PfoI T'CCnGG_A
Cuts at: 0 902 2211
Size: 902 1309
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 436 468 2211
Size: 436 32 1743
Fragments arranged by size:
1743 436 32
PpuMI rG'GwC_Cy
Cuts at: 0 210 1296 2211
Size: 210 1086 915
Fragments arranged by size:
1086 915 210
PspOMI G'GGCC_C
Cuts at: 0 1318 1913 2181 2211
Size: 1318 595 268 30
Fragments arranged by size:
1318 595 268 30
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 256 288 2211
Size: 256 32 1923
Fragments arranged by size:
1923 256 32
PvuII CAG'CTG
Cuts at: 0 222 783 1056 2077 2151 2211
Size: 222 561 273 1021 74 60
Fragments arranged by size:
1021 561 273 222 74 60
SacII CC_GC'GG
Cuts at: 0 290 2211
Size: 290 1921
SapI GCTCTTCn'nnn_
Cuts at: 0 604 697 1384 2211
Size: 604 93 687 827
Fragments arranged by size:
827 687 604 93
SfoI GGC'GCC
Cuts at: 0 1792 2211
Size: 1792 419
SmaI CCC'GGG
Cuts at: 0 742 1804 2211
Size: 742 1062 407
Fragments arranged by size:
1062 742 407
StuI AGG'CCT
Cuts at: 0 64 134 248 799 1681 2211
Size: 64 70 114 551 882 530
Fragments arranged by size:
882 551 530 114 70 64
StyI C'CwwG_G
Cuts at: 0 356 381 466 530 794 1362 2211
Size: 356 25 85 64 264 568 849
Fragments arranged by size:
849 568 356 264 85 64 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 96 422 871 2211
Size: 25 71 326 449 1340
Fragments arranged by size:
1340 449 326 71 25
TatI w'GTAC_w
Cuts at: 0 14 1301 2211
Size: 14 1287 910
Fragments arranged by size:
1287 910 14
XbaI T'CTAG_A
Cuts at: 0 1851 2211
Size: 1851 360
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 363 1896 2211
Size: 363 1533 315
Fragments arranged by size:
1533 363 315
XmaI C'CCGG_G
Cuts at: 0 740 1802 2211
Size: 740 1062 409
Fragments arranged by size:
1062 740 409
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AloI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbeI BcgI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PflMI PfoI PpiI PpuMI PspOMI PsrI PvuII SacII
SapI SfoI SmaI StuI StyI TaqII TatI XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
ApaLI AscI AseI AsiSI BaeI BbsI BbvCI BciVI
BfrBI BmtI BplI Bpu10I BpuEI BsaBI BsiHKAI BsiWI
BspEI BspHI BsrDI BssHII BstBI BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoRV FseI FspI
FspAI HincII HpaI KpnI MfeI MluI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PshAI PsiI
PspXI PstI PvuI RsrII SacI SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XhoI
XmnI ZraI