Restriction map for
pFN21AE4568
(Linear) (Six-base) MAPSORT of: KIEE4568.seq Check: 2506 from: 1 to: 2163
KIEE4568.orf
With 182 enzymes: SgfI *
March 22, 2012 18:24 ..
AatII G_ACGT'C
Cuts at: 0 938 2163
Size: 938 1225
AccI GT'mk_AC
Cuts at: 0 953 2163
Size: 953 1210
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 2163
Size: 340 1823
AflIII A'CryG_T
Cuts at: 0 911 2163
Size: 911 1252
AhdI GACnn_n'nnGTC
Cuts at: 0 656 2163
Size: 656 1507
AleI CACnn'nnGTG
Cuts at: 0 1154 2163
Size: 1154 1009
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 701 733 2163
Size: 701 32 1430
Fragments arranged by size:
1430 701 32
AlwNI CAG_nnn'CTG
Cuts at: 0 752 1233 1539 1785 2163
Size: 752 481 306 246 378
Fragments arranged by size:
752 481 378 306 246
ApaI G_GGCC'C
Cuts at: 0 1315 2163
Size: 1315 848
ApoI r'AATT_y
Cuts at: 0 241 2163
Size: 241 1922
AseI AT'TA_AT
Cuts at: 0 223 2163
Size: 223 1940
AvaI C'yCGr_G
Cuts at: 0 191 803 2163
Size: 191 612 1360
Fragments arranged by size:
1360 612 191
AvrII C'CTAG_G
Cuts at: 0 846 2163
Size: 846 1317
BanI G'GyrC_C
Cuts at: 0 18 332 889 2163
Size: 18 314 557 1274
Fragments arranged by size:
1274 557 314 18
BanII G_rGCy'C
Cuts at: 0 380 492 1090 1315 1339 1888 2163
Size: 380 112 598 225 24 549 275
Fragments arranged by size:
598 549 380 275 225 112 24
BbeI G_GCGC'C
Cuts at: 0 22 2163
Size: 22 2141
BbsI GAAGACnn'nnnn_
Cuts at: 0 599 655 2163
Size: 599 56 1508
Fragments arranged by size:
1508 599 56
BbvCI CC'TCA_GC
Cuts at: 0 1250 2030 2163
Size: 1250 780 133
Fragments arranged by size:
1250 780 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1180 1214 2163
Size: 1180 34 949
Fragments arranged by size:
1180 949 34
BglI GCCn_nnn'nGGC
Cuts at: 0 100 217 1625 2163
Size: 100 117 1408 538
Fragments arranged by size:
1408 538 117 100
Bme1580I G_kGCm'C
Cuts at: 0 894 1315 1857 2163
Size: 894 421 542 306
Fragments arranged by size:
894 542 421 306
BmrI ACTGGGnnnn_n'
Cuts at: 0 400 2163
Size: 400 1763
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 418 1022 2163
Size: 418 604 1141
Fragments arranged by size:
1141 604 418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1232 1343 1822 1902 2038 2163
Size: 1232 111 479 80 136 125
Fragments arranged by size:
1232 479 136 125 111 80
Bpu10I CC'TnA_GC
Cuts at: 0 878 1250 2030 2163
Size: 878 372 780 133
Fragments arranged by size:
878 780 372 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1534 1726 1824 2163
Size: 514 1020 192 98 339
Fragments arranged by size:
1020 514 339 192 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 664 829 2163
Size: 664 165 1334
Fragments arranged by size:
1334 664 165
BsaAI yAC'GTr
Cuts at: 0 870 1794 2163
Size: 870 924 369
Fragments arranged by size:
924 870 369
BsaHI Gr'CG_yC
Cuts at: 0 19 935 2163
Size: 19 916 1228
Fragments arranged by size:
1228 916 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 701 731 2163
Size: 701 30 1432
Fragments arranged by size:
1432 701 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 749 853 1534 1621 1711 2158 2163
Size: 121 628 104 681 87 90 447 5
Fragments arranged by size:
681 628 447 121 104 90 87 5
BseYI C'CCAG_C
Cuts at: 0 568 2163
Size: 568 1595
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 987 2163
Size: 987 1176
BsiEI CG_ry'CG
Cuts at: 0 159 2163
Size: 159 2004
BsiHKAI G_wGCw'C
Cuts at: 0 380 1082 1218 2163
Size: 380 702 136 945
Fragments arranged by size:
945 702 380 136
BsmBI CGTCTCn'nnnn_
Cuts at: 0 504 2163
Size: 504 1659
Bsp1286I G_dGCh'C
Cuts at: 0 380 492 894 1082 1090 1218 1315 1339
Size: 380 112 402 188 8 128 97 24
Cuts at: 1339 1857 1888 2163
Size: 518 31 275
Fragments arranged by size:
518 402 380 275 188 128 112 97
31 24 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1770 2163
Size: 1770 393
BsrGI T'GTAC_A
Cuts at: 0 261 2163
Size: 261 1902
BssHII G'CGCG_C
Cuts at: 0 1911 2163
Size: 1911 252
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1112 2163
Size: 1112 1051
BstBI TT'CG_AA
Cuts at: 0 360 2163
Size: 360 1803
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 2163
Size: 426 1737
BstYI r'GATC_y
Cuts at: 0 842 857 1220 2021 2163
Size: 842 15 363 801 142
Fragments arranged by size:
842 801 363 142 15
Bsu36I CC'TnA_GG
Cuts at: 0 761 1037 1331 2163
Size: 761 276 294 832
Fragments arranged by size:
832 761 294 276
BtgI C'CryG_G
Cuts at: 0 1053 1985 2163
Size: 1053 932 178
Fragments arranged by size:
1053 932 178
ClaI AT'CG_AT
Cuts at: 0 384 2163
Size: 384 1779
DrdI GACnn_nn'nnGTC
Cuts at: 0 1720 2163
Size: 1720 443
EaeI y'GGCC_r
Cuts at: 0 143 209 1988 2163
Size: 143 66 1779 175
Fragments arranged by size:
1779 175 143 66
EarI CTCTTCn'nnn_
Cuts at: 0 1511 1562 2135 2163
Size: 1511 51 573 28
Fragments arranged by size:
1511 573 51 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 118 931 2163
Size: 118 813 1232
Fragments arranged by size:
1232 813 118
EcoICRI GAG'CTC
Cuts at: 0 378 2163
Size: 378 1785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1232 1343 1822 1902 2038 2163
Size: 340 892 111 479 80 136 125
Fragments arranged by size:
892 479 340 136 125 111 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 691 1189 2029 2163
Size: 691 498 840 134
Fragments arranged by size:
840 691 498 134
EcoO109I rG'GnC_Cy
Cuts at: 0 195 1033 2163
Size: 195 838 1130
Fragments arranged by size:
1130 838 195
EcoRI G'AATT_C
Cuts at: 0 241 2163
Size: 241 1922
FspI TGC'GCA
Cuts at: 0 2092 2163
Size: 2092 71
HaeII r_GCGC'y
Cuts at: 0 22 2163
Size: 22 2141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 642 674 1205 1237 1538 1570
Size: 451 32 159 32 531 32 301 32
Cuts at: 1570 2134 2163
Size: 564 29
Fragments arranged by size:
564 531 451 301 159 32 32 32
32 29
KasI G'GCGC_C
Cuts at: 0 18 2163
Size: 18 2145
MfeI C'AATT_G
Cuts at: 0 1110 2163
Size: 1110 1053
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 2163
Size: 926 1237
MscI TGG'CCA
Cuts at: 0 211 1990 2163
Size: 211 1779 173
Fragments arranged by size:
1779 211 173
MslI CAynn'nnrTG
Cuts at: 0 1154 1507 1651 2163
Size: 1154 353 144 512
Fragments arranged by size:
1154 512 353 144
MspA1I CmG'CkG
Cuts at: 0 59 80 922 1686 2016 2106 2163
Size: 59 21 842 764 330 90 57
Fragments arranged by size:
842 764 330 90 59 57 21
NarI GG'CG_CC
Cuts at: 0 19 2163
Size: 19 2144
NcoI C'CATG_G
Cuts at: 0 1053 1985 2163
Size: 1053 932 178
Fragments arranged by size:
1053 932 178
NspI r_CATG'y
Cuts at: 0 867 915 2163
Size: 867 48 1248
Fragments arranged by size:
1248 867 48
PasI CC'CwG_GG
Cuts at: 0 654 2163
Size: 654 1509
PciI A'CATG_T
Cuts at: 0 911 2163
Size: 911 1252
PfoI T'CCnGG_A
Cuts at: 0 1724 1799 2163
Size: 1724 75 364
Fragments arranged by size:
1724 364 75
PmlI CAC'GTG
Cuts at: 0 1794 2163
Size: 1794 369
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 701 733 2163
Size: 701 32 1430
Fragments arranged by size:
1430 701 32
PpuMI rG'GwC_Cy
Cuts at: 0 195 2163
Size: 195 1968
PspOMI G'GGCC_C
Cuts at: 0 1311 2163
Size: 1311 852
PstI C_TGCA'G
Cuts at: 0 1500 1532 2111 2163
Size: 1500 32 579 52
Fragments arranged by size:
1500 579 52 32
PvuI CG_AT'CG
Cuts at: 0 159 2163
Size: 159 2004
PvuII CAG'CTG
Cuts at: 0 922 2016 2106 2163
Size: 922 1094 90 57
Fragments arranged by size:
1094 922 90 57
SacI G_AGCT'C
Cuts at: 0 380 2163
Size: 380 1783
SfcI C'TryA_G
Cuts at: 0 11 1496 1528 1681 2107 2163
Size: 11 1485 32 153 426 56
Fragments arranged by size:
1485 426 153 56 32 11
SfoI GGC'GCC
Cuts at: 0 20 2163
Size: 20 2143
SmlI C'TyrA_G
Cuts at: 0 493 803 1549 1741 1839 2163
Size: 493 310 746 192 98 324
Fragments arranged by size:
746 493 324 310 192 98
StuI AGG'CCT
Cuts at: 0 1879 2163
Size: 1879 284
StyI C'CwwG_G
Cuts at: 0 179 419 846 1053 1985 1991 2163
Size: 179 240 427 207 932 6 172
Fragments arranged by size:
932 427 240 207 179 172 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 590 1009 2163
Size: 590 419 1154
Fragments arranged by size:
1154 590 419
TatI w'GTAC_w
Cuts at: 0 261 914 2163
Size: 261 653 1249
Fragments arranged by size:
1249 653 261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1271 1303 2163
Size: 1271 32 860
Fragments arranged by size:
1271 860 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1998 2163
Size: 1998 165
XhoI C'TCGA_G
Cuts at: 0 803 2163
Size: 803 1360
XmnI GAAnn'nnTTC
Cuts at: 0 2039 2163
Size: 2039 124
ZraI GAC'GTC
Cuts at: 0 936 2163
Size: 936 1227
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AleI AloI AlwNI
ApaI ApoI AseI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BcgI BglI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrGI BssHII
BstAPI BstBI BstXI BstYI Bsu36I BtgI ClaI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I KasI MfeI MmeI MscI MslI
MspA1I NarI NcoI NspI PasI PciI PfoI PmlI
PpiI PpuMI PspOMI PstI PvuI PvuII SacI SfcI
SfoI SmlI StuI StyI TaqII TatI TstI XcmI
XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AlfI ApaLI
AscI AsiSI BaeI BamHI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BsrBI BsrDI BsrFI BssSI BstEII BstZ17I BtgZI
BtsI CspCI DraI DraIII EagI EcoRV FalI FseI
FspAI HincII HindIII HpaI KpnI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI
PshAI PsiI PspXI PsrI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XmaI