Restriction map for
pFN21AE4567
(Linear) (Six-base) MAPSORT of: KIEE4567.seq Check: 26 from: 1 to: 2157
KIEE4567.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AccI GT'mk_AC
Cuts at: 0 1609 2157
Size: 1609 548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 1577 2157
Size: 566 1011 580
Fragments arranged by size:
1011 580 566
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2147 2157
Size: 2147 10
AlwNI CAG_nnn'CTG
Cuts at: 0 856 2157
Size: 856 1301
ApoI r'AATT_y
Cuts at: 0 284 304 1061 1273 1311 1633 1911 2027
Size: 284 20 757 212 38 322 278 116
Cuts at: 2027 2157
Size: 130
Fragments arranged by size:
757 322 284 278 212 130 116 38
20
AseI AT'TA_AT
Cuts at: 0 392 1349 2157
Size: 392 957 808
Fragments arranged by size:
957 808 392
BbsI GAAGACnn'nnnn_
Cuts at: 0 554 856 1273 1873 2157
Size: 554 302 417 600 284
Fragments arranged by size:
600 554 417 302 284
BciVI GTATCCnnnnn_n'
Cuts at: 0 886 2157
Size: 886 1271
BglI GCCn_nnn'nGGC
Cuts at: 0 655 2157
Size: 655 1502
BlpI GC'TnA_GC
Cuts at: 0 933 2157
Size: 933 1224
Bme1580I G_kGCm'C
Cuts at: 0 977 2157
Size: 977 1180
BmrI ACTGGGnnnn_n'
Cuts at: 0 288 2157
Size: 288 1869
BmtI G_CTAG'C
Cuts at: 0 331 1762 2157
Size: 331 1431 395
Fragments arranged by size:
1431 395 331
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 694 817 982 2157
Size: 694 123 165 1175
Fragments arranged by size:
1175 694 165 123
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 767 975 1933 2157
Size: 428 339 208 958 224
Fragments arranged by size:
958 428 339 224 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 2157
Size: 482 1675
BsaI GGTCTCn'nnnn_
Cuts at: 0 788 1224 2157
Size: 788 436 933
Fragments arranged by size:
933 788 436
BsaBI GATnn'nnATC
Cuts at: 0 1033 2157
Size: 1033 1124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 740 770 896 926 948 978 1163 1193
Size: 740 30 126 30 22 30 185 30
Cuts at: 1193 1733 1763 2157
Size: 540 30 394
Fragments arranged by size:
740 540 394 185 126 30 30 30
30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 431 485 1103 1212 1321 1754 2157
Size: 91 340 54 618 109 109 433 403
Fragments arranged by size:
618 433 403 340 109 109 91 54
BseYI C'CCAG_C
Cuts at: 0 67 650 816 1259 2157
Size: 67 583 166 443 898
Fragments arranged by size:
898 583 443 166 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 2143 2157
Size: 809 1334 14
Fragments arranged by size:
1334 809 14
BsiHKAI G_wGCw'C
Cuts at: 0 1605 1778 2157
Size: 1605 173 379
Fragments arranged by size:
1605 379 173
BsmI GAATG_Cn'
Cuts at: 0 509 2157
Size: 509 1648
Bsp1286I G_dGCh'C
Cuts at: 0 977 1605 1778 2157
Size: 977 628 173 379
Fragments arranged by size:
977 628 379 173
BspHI T'CATG_A
Cuts at: 0 1185 2157
Size: 1185 972
BsrGI T'GTAC_A
Cuts at: 0 1396 1409 2157
Size: 1396 13 748
Fragments arranged by size:
1396 748 13
BssHII G'CGCG_C
Cuts at: 0 43 2157
Size: 43 2114
BstEII G'GTnAC_C
Cuts at: 0 811 1126 2157
Size: 811 315 1031
Fragments arranged by size:
1031 811 315
BstXI CCAn_nnnn'nTGG
Cuts at: 0 734 1582 2157
Size: 734 848 575
Fragments arranged by size:
848 734 575
BstYI r'GATC_y
Cuts at: 0 1546 2157
Size: 1546 611
BstZ17I GTA'TAC
Cuts at: 0 1610 2157
Size: 1610 547
BtsI GCAGTG_nn'
Cuts at: 0 1100 2157
Size: 1100 1057
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 277 312 2157
Size: 277 35 1845
Fragments arranged by size:
1845 277 35
DraI TTT'AAA
Cuts at: 0 558 1710 2157
Size: 558 1152 447
Fragments arranged by size:
1152 558 447
DrdI GACnn_nn'nnGTC
Cuts at: 0 1111 2157
Size: 1111 1046
EaeI y'GGCC_r
Cuts at: 0 35 2157
Size: 35 2122
EarI CTCTTCn'nnn_
Cuts at: 0 118 2086 2157
Size: 118 1968 71
Fragments arranged by size:
1968 118 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 566 767 975 1577 1933 2157
Size: 428 138 201 208 602 356 224
Fragments arranged by size:
602 428 356 224 208 201 138
EcoNI CCTnn'n_nnAGG
Cuts at: 0 443 1454 2157
Size: 443 1011 703
Fragments arranged by size:
1011 703 443
EcoRI G'AATT_C
Cuts at: 0 1311 2157
Size: 1311 846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 234 2157
Size: 234 1923
HaeII r_GCGC'y
Cuts at: 0 76 2157
Size: 76 2081
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 740 772 948 980 1065 1097 2157
Size: 740 32 176 32 85 32 1060
Fragments arranged by size:
1060 740 176 85 32 32 32
HincII GTy'rAC
Cuts at: 0 531 2157
Size: 531 1626
HpaI GTT'AAC
Cuts at: 0 531 2157
Size: 531 1626
MfeI C'AATT_G
Cuts at: 0 582 887 2157
Size: 582 305 1270
Fragments arranged by size:
1270 582 305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 715 966 1468 1599 2157
Size: 710 5 251 502 131 558
Fragments arranged by size:
710 558 502 251 131 5
MscI TGG'CCA
Cuts at: 0 37 2157
Size: 37 2120
MslI CAynn'nnrTG
Cuts at: 0 338 2157
Size: 338 1819
MspA1I CmG'CkG
Cuts at: 0 654 953 1177 1404 1640 2131 2157
Size: 654 299 224 227 236 491 26
Fragments arranged by size:
654 491 299 236 227 224 26
NdeI CA'TA_TG
Cuts at: 0 1674 2157
Size: 1674 483
NheI G'CTAG_C
Cuts at: 0 327 1758 2157
Size: 327 1431 399
Fragments arranged by size:
1431 399 327
NspI r_CATG'y
Cuts at: 0 1527 1975 2157
Size: 1527 448 182
Fragments arranged by size:
1527 448 182
PflMI CCAn_nnn'nTGG
Cuts at: 0 2078 2157
Size: 2078 79
PfoI T'CCnGG_A
Cuts at: 0 444 744 1949 2157
Size: 444 300 1205 208
Fragments arranged by size:
1205 444 300 208
PsiI TTA'TAA
Cuts at: 0 127 2157
Size: 127 2030
PstI C_TGCA'G
Cuts at: 0 1905 2136 2157
Size: 1905 231 21
Fragments arranged by size:
1905 231 21
PvuII CAG'CTG
Cuts at: 0 654 953 1177 2131 2157
Size: 654 299 224 954 26
Fragments arranged by size:
954 654 299 224 26
SexAI A'CCwGG_T
Cuts at: 0 807 2157
Size: 807 1350
SfcI C'TryA_G
Cuts at: 0 1364 1802 1833 1901 2132 2157
Size: 1364 438 31 68 231 25
Fragments arranged by size:
1364 438 231 68 31 25
SmlI C'TyrA_G
Cuts at: 0 497 2157
Size: 497 1660
SphI G_CATG'C
Cuts at: 0 1527 2157
Size: 1527 630
StuI AGG'CCT
Cuts at: 0 2077 2157
Size: 2077 80
StyI C'CwwG_G
Cuts at: 0 2072 2157
Size: 2072 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 916 1131 2157
Size: 916 215 1026
Fragments arranged by size:
1026 916 215
TatI w'GTAC_w
Cuts at: 0 1396 1409 2157
Size: 1396 13 748
Fragments arranged by size:
1396 748 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 896 928 1733 1765 2157
Size: 896 32 805 32 392
Fragments arranged by size:
896 805 392 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 950 2157
Size: 950 1207
XmnI GAAnn'nnTTC
Cuts at: 0 622 1008 1590 2157
Size: 622 386 582 567
Fragments arranged by size:
622 582 567 386
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApoI AseI BbsI BciVI
BglI BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrGI BssHII BstEII BstXI BstYI BstZ17I
BtsI CspCI DraI DrdI EaeI EarI Eco57MI EcoNI
EcoRI FalI HaeII Hin4I HincII HpaI MfeI MmeI
MscI MslI MspA1I NdeI NheI NspI PflMI PfoI
PsiI PstI PvuII SexAI SfcI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BclI BfrBI BglII BmgBI Bpu10I BsaAI BsaHI BsaWI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI Bsu36I BtgI BtgZI ClaI DraIII
EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI
HindIII KasI KpnI MluI NaeI NarI NcoI NgoMIV
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI ZraI