Restriction map for
pFN21AE4566
(Linear) (Six-base) MAPSORT of: KIEE4566.seq Check: 1617 from: 1 to: 2130
KIEE4566.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AatII G_ACGT'C
Cuts at: 0 1070 2130
Size: 1070 1060
AccI GT'mk_AC
Cuts at: 0 2117 2130
Size: 2117 13
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 936 983 1771 1951 1969 2130
Size: 850 86 47 788 180 18 161
Fragments arranged by size:
850 788 180 161 86 47 18
AflII C'TTAA_G
Cuts at: 0 1114 2130
Size: 1114 1016
AflIII A'CryG_T
Cuts at: 0 23 2084 2130
Size: 23 2061 46
Fragments arranged by size:
2061 46 23
AleI CACnn'nnGTG
Cuts at: 0 1484 2130
Size: 1484 646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2023 2130
Size: 2023 107
ApoI r'AATT_y
Cuts at: 0 68 2130
Size: 68 2062
AseI AT'TA_AT
Cuts at: 0 1649 2130
Size: 1649 481
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1143 1176 2130
Size: 1143 33 954
Fragments arranged by size:
1143 954 33
BamHI G'GATC_C
Cuts at: 0 957 2130
Size: 957 1173
BanI G'GyrC_C
Cuts at: 0 478 2105 2130
Size: 478 1627 25
Fragments arranged by size:
1627 478 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 101 2130
Size: 101 2029
BciVI GTATCCnnnnn_n'
Cuts at: 0 1501 2130
Size: 1501 629
BclI T'GATC_A
Cuts at: 0 938 1610 2130
Size: 938 672 520
Fragments arranged by size:
938 672 520
BmgBI CAC'GTC
Cuts at: 0 24 1538 2130
Size: 24 1514 592
Fragments arranged by size:
1514 592 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 456 2130
Size: 456 1674
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2094 2130
Size: 2094 36
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1193 1270 2130
Size: 1193 77 860
Fragments arranged by size:
1193 860 77
Bpu10I CC'TnA_GC
Cuts at: 0 992 2130
Size: 992 1138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 2130
Size: 181 1949
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 1692 2130
Size: 31 1661 438
Fragments arranged by size:
1661 438 31
BsaAI yAC'GTr
Cuts at: 0 876 2019 2130
Size: 876 1143 111
Fragments arranged by size:
1143 876 111
BsaHI Gr'CG_yC
Cuts at: 0 1067 2130
Size: 1067 1063
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1258 1529 1894 2130
Size: 1258 271 365 236
Fragments arranged by size:
1258 365 271 236
BsiHKAI G_wGCw'C
Cuts at: 0 1110 1804 2130
Size: 1110 694 326
Fragments arranged by size:
1110 694 326
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1528 2130
Size: 1528 602
Bsp1286I G_dGCh'C
Cuts at: 0 1110 1804 2130
Size: 1110 694 326
Fragments arranged by size:
1110 694 326
BspHI T'CATG_A
Cuts at: 0 464 2130
Size: 464 1666
BsrBI CCG'CTC
Cuts at: 0 1128 2130
Size: 1128 1002
BsrDI GCAATG_nn'
Cuts at: 0 1846 1955 2130
Size: 1846 109 175
Fragments arranged by size:
1846 175 109
BsrGI T'GTAC_A
Cuts at: 0 303 2130
Size: 303 1827
BstEII G'GTnAC_C
Cuts at: 0 1272 2130
Size: 1272 858
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1044 2130
Size: 1044 1086
BstYI r'GATC_y
Cuts at: 0 957 2130
Size: 957 1173
BtgI C'CryG_G
Cuts at: 0 2037 2130
Size: 2037 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 801 2082 2130
Size: 801 1281 48
Fragments arranged by size:
1281 801 48
BtsI GCAGTG_nn'
Cuts at: 0 172 1505 1907 2130
Size: 172 1333 402 223
Fragments arranged by size:
1333 402 223 172
DrdI GACnn_nn'nnGTC
Cuts at: 0 1930 2130
Size: 1930 200
EaeI y'GGCC_r
Cuts at: 0 729 1502 1559 1773 2130
Size: 729 773 57 214 357
Fragments arranged by size:
773 729 357 214 57
EarI CTCTTCn'nnn_
Cuts at: 0 959 2130
Size: 959 1171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 850 936 983 1193 1270 1771 1951 1969
Size: 850 86 47 210 77 501 180 18
Cuts at: 1969 2130
Size: 161
Fragments arranged by size:
850 501 210 180 161 86 77 47
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 799 2130
Size: 799 1331
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1691 1723 2130
Size: 1691 32 407
Fragments arranged by size:
1691 407 32
HincII GTy'rAC
Cuts at: 0 2118 2130
Size: 2118 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1061 1713 2130
Size: 1061 652 417
Fragments arranged by size:
1061 652 417
MscI TGG'CCA
Cuts at: 0 1504 1561 2130
Size: 1504 57 569
Fragments arranged by size:
1504 569 57
MslI CAynn'nnrTG
Cuts at: 0 1484 1547 2130
Size: 1484 63 583
Fragments arranged by size:
1484 583 63
MspA1I CmG'CkG
Cuts at: 0 521 1157 2130
Size: 521 636 973
Fragments arranged by size:
973 636 521
NspI r_CATG'y
Cuts at: 0 1749 2088 2130
Size: 1749 339 42
Fragments arranged by size:
1749 339 42
PciI A'CATG_T
Cuts at: 0 2084 2130
Size: 2084 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 2130
Size: 642 1488
PfoI T'CCnGG_A
Cuts at: 0 338 2130
Size: 338 1792
PmlI CAC'GTG
Cuts at: 0 2019 2130
Size: 2019 111
PstI C_TGCA'G
Cuts at: 0 1085 2130
Size: 1085 1045
PvuII CAG'CTG
Cuts at: 0 521 2130
Size: 521 1609
SalI G'TCGA_C
Cuts at: 0 2116 2130
Size: 2116 14
ScaI AGT'ACT
Cuts at: 0 983 2130
Size: 983 1147
SfcI C'TryA_G
Cuts at: 0 796 1081 1449 2130
Size: 796 285 368 681
Fragments arranged by size:
796 681 368 285
SmlI C'TyrA_G
Cuts at: 0 160 1114 2130
Size: 160 954 1016
Fragments arranged by size:
1016 954 160
SspI AAT'ATT
Cuts at: 0 412 2130
Size: 412 1718
StuI AGG'CCT
Cuts at: 0 121 1670 2130
Size: 121 1549 460
Fragments arranged by size:
1549 460 121
StyI C'CwwG_G
Cuts at: 0 2027 2130
Size: 2027 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 438 694 1458 1474 1787 2130
Size: 438 256 764 16 313 343
Fragments arranged by size:
764 438 343 313 256 16
TatI w'GTAC_w
Cuts at: 0 303 981 1970 2130
Size: 303 678 989 160
Fragments arranged by size:
989 678 303 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1011 1043 2130
Size: 1011 32 1087
Fragments arranged by size:
1087 1011 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 336 1245 2130
Size: 336 909 885
Fragments arranged by size:
909 885 336
ZraI GAC'GTC
Cuts at: 0 1068 2130
Size: 1068 1062
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AleI AlfI ApoI
AseI BaeI BamHI BanI BbsI BciVI BclI BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BseRI BsiHKAI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrGI
BstEII BstXI BstYI BtgI BtgZI BtsI DrdI EaeI
EarI Eco57MI EcoNI Hin4I HincII MmeI MscI MslI
MspA1I NspI PciI PflMI PfoI PmlI PstI PvuII
SalI ScaI SfcI SmlI SspI StuI StyI TaqII
TatI TstI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AloI AlwNI
ApaI ApaLI AscI AsiSI AvaI AvrII BanII BbeI
BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmtI
BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI
BspEI BspMI BsrFI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I ClaI CspCI DraI DraIII EagI EciI EcoICRI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI