Restriction map for
pFN21AE4565
(Linear) (Six-base) MAPSORT of: KIEE4565.seq Check: 6248 from: 1 to: 2106
KIEE4565.orf
With 182 enzymes: SgfI *
March 22, 2012 18:23 ..
AccI GT'mk_AC
Cuts at: 0 818 1382 1498 2106
Size: 818 564 116 608
Fragments arranged by size:
818 608 564 116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 2068 2106
Size: 818 1250 38
Fragments arranged by size:
1250 818 38
AleI CACnn'nnGTG
Cuts at: 0 239 2106
Size: 239 1867
AlwNI CAG_nnn'CTG
Cuts at: 0 1986 2106
Size: 1986 120
ApaI G_GGCC'C
Cuts at: 0 276 2106
Size: 276 1830
ApoI r'AATT_y
Cuts at: 0 161 593 614 882 1624 1933 2106
Size: 161 432 21 268 742 309 173
Fragments arranged by size:
742 432 309 268 173 161 21
AvaI C'yCGr_G
Cuts at: 0 31 276 2106
Size: 31 245 1830
Fragments arranged by size:
1830 245 31
BamHI G'GATC_C
Cuts at: 0 415 534 2106
Size: 415 119 1572
Fragments arranged by size:
1572 415 119
BanII G_rGCy'C
Cuts at: 0 276 2106
Size: 276 1830
BbsI GAAGACnn'nnnn_
Cuts at: 0 1364 1852 2106
Size: 1364 488 254
Fragments arranged by size:
1364 488 254
BbvCI CC'TCA_GC
Cuts at: 0 1232 2106
Size: 1232 874
BciVI GTATCCnnnnn_n'
Cuts at: 0 518 685 2106
Size: 518 167 1421
Fragments arranged by size:
1421 518 167
BclI T'GATC_A
Cuts at: 0 1440 2106
Size: 1440 666
BglI GCCn_nnn'nGGC
Cuts at: 0 64 2106
Size: 64 2042
BglII A'GATC_T
Cuts at: 0 455 2106
Size: 455 1651
Bme1580I G_kGCm'C
Cuts at: 0 77 276 2106
Size: 77 199 1830
Fragments arranged by size:
1830 199 77
BmgBI CAC'GTC
Cuts at: 0 72 2106
Size: 72 2034
BmrI ACTGGGnnnn_n'
Cuts at: 0 540 2106
Size: 540 1566
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2092 2106
Size: 2092 14
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 323 972 1514 2106
Size: 115 208 649 542 592
Fragments arranged by size:
649 592 542 208 115
Bpu10I CC'TnA_GC
Cuts at: 0 1232 2106
Size: 1232 874
BsaAI yAC'GTr
Cuts at: 0 1809 2106
Size: 1809 297
BsaBI GATnn'nnATC
Cuts at: 0 715 2106
Size: 715 1391
BsaHI Gr'CG_yC
Cuts at: 0 230 2034 2106
Size: 230 1804 72
Fragments arranged by size:
1804 230 72
BseYI C'CCAG_C
Cuts at: 0 992 2106
Size: 992 1114
BsiHKAI G_wGCw'C
Cuts at: 0 1264 2106
Size: 1264 842
BsmI GAATG_Cn'
Cuts at: 0 561 701 1253 2106
Size: 561 140 552 853
Fragments arranged by size:
853 561 552 140
Bsp1286I G_dGCh'C
Cuts at: 0 77 276 1264 2106
Size: 77 199 988 842
Fragments arranged by size:
988 842 199 77
BspHI T'CATG_A
Cuts at: 0 954 2106
Size: 954 1152
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 1846 2106
Size: 131 1715 260
Fragments arranged by size:
1715 260 131
BsrDI GCAATG_nn'
Cuts at: 0 669 1418 1838 1921 2106
Size: 669 749 420 83 185
Fragments arranged by size:
749 669 420 185 83
BsrGI T'GTAC_A
Cuts at: 0 2054 2106
Size: 2054 52
BssSI C'ACGA_G
Cuts at: 0 403 2106
Size: 403 1703
BstEII G'GTnAC_C
Cuts at: 0 748 2106
Size: 748 1358
BstXI CCAn_nnnn'nTGG
Cuts at: 0 93 1325 2106
Size: 93 1232 781
Fragments arranged by size:
1232 781 93
BstYI r'GATC_y
Cuts at: 0 415 455 534 2094 2106
Size: 415 40 79 1560 12
Fragments arranged by size:
1560 415 79 40 12
BstZ17I GTA'TAC
Cuts at: 0 1383 1499 2106
Size: 1383 116 607
Fragments arranged by size:
1383 607 116
BtgI C'CryG_G
Cuts at: 0 20 125 475 580 833 1098 1307 2106
Size: 20 105 350 105 253 265 209 799
Fragments arranged by size:
799 350 265 253 209 105 105 20
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 470 505 2051 2086 2106
Size: 470 35 1546 35 20
Fragments arranged by size:
1546 470 35 35 20
DraI TTT'AAA
Cuts at: 0 1350 2106
Size: 1350 756
DraIII CAC_nnn'GTG
Cuts at: 0 1208 2106
Size: 1208 898
EaeI y'GGCC_r
Cuts at: 0 83 2106
Size: 83 2023
EarI CTCTTCn'nnn_
Cuts at: 0 794 2106
Size: 794 1312
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 323 818 972 1514 2068 2106
Size: 115 208 495 154 542 554 38
Fragments arranged by size:
554 542 495 208 154 115 38
EcoO109I rG'GnC_Cy
Cuts at: 0 273 2106
Size: 273 1833
HaeII r_GCGC'y
Cuts at: 0 52 1404 2106
Size: 52 1352 702
Fragments arranged by size:
1352 702 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 447 479 942 974 1225 1257 1425 1457
Size: 447 32 463 32 251 32 168 32
Cuts at: 1457 2106
Size: 649
Fragments arranged by size:
649 463 447 251 168 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 762 2106
Size: 762 1344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 1016 1893 2106
Size: 298 718 877 213
Fragments arranged by size:
877 718 298 213
MscI TGG'CCA
Cuts at: 0 85 2106
Size: 85 2021
MslI CAynn'nnrTG
Cuts at: 0 239 548 869 1097 2106
Size: 239 309 321 228 1009
Fragments arranged by size:
1009 321 309 239 228
MspA1I CmG'CkG
Cuts at: 0 22 992 1963 2106
Size: 22 970 971 143
Fragments arranged by size:
971 970 143 22
NcoI C'CATG_G
Cuts at: 0 833 1098 1307 2106
Size: 833 265 209 799
Fragments arranged by size:
833 799 265 209
NdeI CA'TA_TG
Cuts at: 0 549 2106
Size: 549 1557
NspI r_CATG'y
Cuts at: 0 662 730 2106
Size: 662 68 1376
Fragments arranged by size:
1376 662 68
PasI CC'CwG_GG
Cuts at: 0 1275 2106
Size: 1275 831
PflMI CCAn_nnn'nTGG
Cuts at: 0 1104 2106
Size: 1104 1002
PfoI T'CCnGG_A
Cuts at: 0 92 2106
Size: 92 2014
PsiI TTA'TAA
Cuts at: 0 647 2106
Size: 647 1459
PspOMI G'GGCC_C
Cuts at: 0 272 2106
Size: 272 1834
PvuII CAG'CTG
Cuts at: 0 992 2106
Size: 992 1114
SacII CC_GC'GG
Cuts at: 0 23 2106
Size: 23 2083
SapI GCTCTTCn'nnn_
Cuts at: 0 794 2106
Size: 794 1312
SmaI CCC'GGG
Cuts at: 0 278 2106
Size: 278 1828
SpeI A'CTAG_T
Cuts at: 0 439 2106
Size: 439 1667
SphI G_CATG'C
Cuts at: 0 730 2106
Size: 730 1376
SspI AAT'ATT
Cuts at: 0 1138 2106
Size: 1138 968
StuI AGG'CCT
Cuts at: 0 686 1804 2106
Size: 686 1118 302
Fragments arranged by size:
1118 686 302
StyI C'CwwG_G
Cuts at: 0 687 833 1098 1307 2106
Size: 687 146 265 209 799
Fragments arranged by size:
799 687 265 209 146
TatI w'GTAC_w
Cuts at: 0 399 666 2054 2106
Size: 399 267 1388 52
Fragments arranged by size:
1388 399 267 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1194 1226 2106
Size: 1194 32 880
Fragments arranged by size:
1194 880 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1282 2106
Size: 1282 824
XmaI C'CCGG_G
Cuts at: 0 276 2106
Size: 276 1830
XmnI GAAnn'nnTTC
Cuts at: 0 618 2106
Size: 618 1488
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaI ApoI AvaI BamHI
BanII BbsI BbvCI BciVI BclI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaBI BsaHI
BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BssSI BstEII BstXI BstYI BstZ17I BtgI CspCI DraI
DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NcoI NdeI NspI PasI
PflMI PfoI PsiI PspOMI PvuII SacII SapI SmaI
SpeI SphI SspI StuI StyI TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BcgI BfrBI BlpI BmtI BpuEI
BsaI BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BstAPI BstBI Bsu36I BtgZI
BtsI ClaI DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PspXI PsrI PstI PvuI RsrII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmlI SnaBI SrfI SwaI TaqII Tth111I XbaI
XhoI ZraI