Restriction map for
pFN21AE4561
(Linear) (Six-base) MAPSORT of: KIEE4561.seq Check: 6540 from: 1 to: 2055
KIEE4561.orf
With 182 enzymes: SgfI *
March 22, 2012 18:23 ..
AccI GT'mk_AC
Cuts at: 0 386 2055
Size: 386 1669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 595 766 919 1024 2055
Size: 556 39 171 153 105 1031
Fragments arranged by size:
1031 556 171 153 105 39
AflII C'TTAA_G
Cuts at: 0 2047 2055
Size: 2047 8
AleI CACnn'nnGTG
Cuts at: 0 430 1580 2055
Size: 430 1150 475
Fragments arranged by size:
1150 475 430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 159 2055
Size: 159 1896
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 691 723 2055
Size: 691 32 1332
Fragments arranged by size:
1332 691 32
AlwNI CAG_nnn'CTG
Cuts at: 0 420 529 567 771 897 1119 1506 2055
Size: 420 109 38 204 126 222 387 549
Fragments arranged by size:
549 420 387 222 204 126 109 38
ApoI r'AATT_y
Cuts at: 0 1223 2055
Size: 1223 832
AvaI C'yCGr_G
Cuts at: 0 105 2055
Size: 105 1950
BanI G'GyrC_C
Cuts at: 0 1174 2055
Size: 1174 881
BanII G_rGCy'C
Cuts at: 0 1801 1905 2055
Size: 1801 104 150
Fragments arranged by size:
1801 150 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 287 2055
Size: 287 1768
BbvCI CC'TCA_GC
Cuts at: 0 67 950 1803 1961 2055
Size: 67 883 853 158 94
Fragments arranged by size:
883 853 158 94 67
BciVI GTATCCnnnnn_n'
Cuts at: 0 579 2055
Size: 579 1476
BclI T'GATC_A
Cuts at: 0 422 2055
Size: 422 1633
BglI GCCn_nnn'nGGC
Cuts at: 0 1741 2055
Size: 1741 314
BlpI GC'TnA_GC
Cuts at: 0 761 878 1235 1795 2055
Size: 761 117 357 560 260
Fragments arranged by size:
761 560 357 260 117
BmgBI CAC'GTC
Cuts at: 0 1428 2055
Size: 1428 627
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1013 1868 1983 2055
Size: 1013 855 115 72
Fragments arranged by size:
1013 855 115 72
Bpu10I CC'TnA_GC
Cuts at: 0 67 950 1803 1961 2055
Size: 67 883 853 158 94
Fragments arranged by size:
883 853 158 94 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1002 2055
Size: 616 386 1053
Fragments arranged by size:
1053 616 386
BsaI GGTCTCn'nnnn_
Cuts at: 0 448 1703 2055
Size: 448 1255 352
Fragments arranged by size:
1255 448 352
BsaAI yAC'GTr
Cuts at: 0 1185 2055
Size: 1185 870
BsaBI GATnn'nnATC
Cuts at: 0 1214 1300 2055
Size: 1214 86 755
Fragments arranged by size:
1214 755 86
BsaWI w'CCGG_w
Cuts at: 0 461 548 2055
Size: 461 87 1507
Fragments arranged by size:
1507 461 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 691 721 1438 1468 1866 1896
Size: 343 30 318 30 717 30 398 30
Cuts at: 1896 2055
Size: 159
Fragments arranged by size:
717 398 343 318 159 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 34 136 466 596 823 967 970
Size: 22 12 102 330 130 227 144 3
Cuts at: 970 1459 1601 1978 2055
Size: 489 142 377 77
Fragments arranged by size:
489 377 330 227 144 142 130 102
77 22 12 3
BseYI C'CCAG_C
Cuts at: 0 890 1372 1855 2055
Size: 890 482 483 200
Fragments arranged by size:
890 483 482 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 778 1264 2055
Size: 34 744 486 791
Fragments arranged by size:
791 744 486 34
BsiHKAI G_wGCw'C
Cuts at: 0 596 719 815 2055
Size: 596 123 96 1240
Fragments arranged by size:
1240 596 123 96
BsmI GAATG_Cn'
Cuts at: 0 1928 2055
Size: 1928 127
Bsp1286I G_dGCh'C
Cuts at: 0 596 719 815 1801 1905 2055
Size: 596 123 96 986 104 150
Fragments arranged by size:
986 596 150 123 104 96
BspEI T'CCGG_A
Cuts at: 0 461 2055
Size: 461 1594
BspHI T'CATG_A
Cuts at: 0 1415 2055
Size: 1415 640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 957 2055
Size: 957 1098
BsrBI CCG'CTC
Cuts at: 0 930 2055
Size: 930 1125
BsrDI GCAATG_nn'
Cuts at: 0 1264 2055
Size: 1264 791
BsrFI r'CCGG_y
Cuts at: 0 1670 2055
Size: 1670 385
BstAPI GCAn_nnn'nTGC
Cuts at: 0 567 771 2055
Size: 567 204 1284
Fragments arranged by size:
1284 567 204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1380 1942 2055
Size: 1380 562 113
Fragments arranged by size:
1380 562 113
BstYI r'GATC_y
Cuts at: 0 1301 1531 2055
Size: 1301 230 524
Fragments arranged by size:
1301 524 230
BstZ17I GTA'TAC
Cuts at: 0 387 2055
Size: 387 1668
Bsu36I CC'TnA_GG
Cuts at: 0 274 2055
Size: 274 1781
BtgI C'CryG_G
Cuts at: 0 324 398 1761 2055
Size: 324 74 1363 294
Fragments arranged by size:
1363 324 294 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 875 2055
Size: 875 1180
BtsI GCAGTG_nn'
Cuts at: 0 128 1497 2055
Size: 128 1369 558
Fragments arranged by size:
1369 558 128
DraIII CAC_nnn'GTG
Cuts at: 0 1816 2055
Size: 1816 239
EaeI y'GGCC_r
Cuts at: 0 480 1124 1733 2055
Size: 480 644 609 322
Fragments arranged by size:
644 609 480 322
EarI CTCTTCn'nnn_
Cuts at: 0 1150 1203 2055
Size: 1150 53 852
Fragments arranged by size:
1150 852 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 595 766 919 1013 1024 1868 1983
Size: 556 39 171 153 94 11 844 115
Cuts at: 1983 2055
Size: 72
Fragments arranged by size:
844 556 171 153 115 94 72 39
11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 361 2055
Size: 52 309 1694
Fragments arranged by size:
1694 309 52
EcoO109I rG'GnC_Cy
Cuts at: 0 354 1035 2055
Size: 354 681 1020
Fragments arranged by size:
1020 681 354
HaeII r_GCGC'y
Cuts at: 0 1278 1506 1756 2055
Size: 1278 228 250 299
Fragments arranged by size:
1278 299 250 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 991 1023 1438 1440 1470 1472 1549 1581
Size: 991 32 415 2 30 2 77 32
Cuts at: 1581 1864 1896 1960 1992 2055
Size: 283 32 64 32 63
Fragments arranged by size:
991 415 283 77 64 63 32 32
32 32 30 2 2
HincII GTy'rAC
Cuts at: 0 1326 2055
Size: 1326 729
MfeI C'AATT_G
Cuts at: 0 390 2055
Size: 390 1665
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 2055
Size: 941 1114
MscI TGG'CCA
Cuts at: 0 482 1126 1735 2055
Size: 482 644 609 320
Fragments arranged by size:
644 609 482 320
MslI CAynn'nnrTG
Cuts at: 0 346 430 517 646 1580 2055
Size: 346 84 87 129 934 475
Fragments arranged by size:
934 475 346 129 87 84
MspA1I CmG'CkG
Cuts at: 0 573 894 1056 1116 1286 1376 2055
Size: 573 321 162 60 170 90 679
Fragments arranged by size:
679 573 321 170 162 90 60
NcoI C'CATG_G
Cuts at: 0 324 1761 2055
Size: 324 1437 294
Fragments arranged by size:
1437 324 294
NdeI CA'TA_TG
Cuts at: 0 429 2055
Size: 429 1626
PasI CC'CwG_GG
Cuts at: 0 1693 2055
Size: 1693 362
PflMI CCAn_nnn'nTGG
Cuts at: 0 1347 1522 1596 1767 1816 2055
Size: 1347 175 74 171 49 239
Fragments arranged by size:
1347 239 175 171 74 49
PmlI CAC'GTG
Cuts at: 0 1185 2055
Size: 1185 870
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 2055
Size: 691 32 1332
Fragments arranged by size:
1332 691 32
PpuMI rG'GwC_Cy
Cuts at: 0 354 2055
Size: 354 1701
PshAI GACnn'nnGTC
Cuts at: 0 551 2055
Size: 551 1504
PstI C_TGCA'G
Cuts at: 0 135 473 572 1055 1487 1632 2055
Size: 135 338 99 483 432 145 423
Fragments arranged by size:
483 432 423 338 145 135 99
PvuII CAG'CTG
Cuts at: 0 573 1056 1116 1376 2055
Size: 573 483 60 260 679
Fragments arranged by size:
679 573 483 260 60
SapI GCTCTTCn'nnn_
Cuts at: 0 1203 2055
Size: 1203 852
SexAI A'CCwGG_T
Cuts at: 0 1812 2055
Size: 1812 243
SfcI C'TryA_G
Cuts at: 0 131 469 568 1051 1483 1628 2055
Size: 131 338 99 483 432 145 427
Fragments arranged by size:
483 432 427 338 145 131 99
SmlI C'TyrA_G
Cuts at: 0 631 981 2047 2055
Size: 631 350 1066 8
Fragments arranged by size:
1066 631 350 8
SspI AAT'ATT
Cuts at: 0 1081 2055
Size: 1081 974
StuI AGG'CCT
Cuts at: 0 979 2055
Size: 979 1076
StyI C'CwwG_G
Cuts at: 0 324 493 710 1127 1761 2055
Size: 324 169 217 417 634 294
Fragments arranged by size:
634 417 324 294 217 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1706 2055
Size: 1706 349
TatI w'GTAC_w
Cuts at: 0 1387 2055
Size: 1387 668
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 343 375 1183 1215 1866 1898 1914 1946
Size: 343 32 808 32 651 32 16 32
Cuts at: 1946 2055
Size: 109
Fragments arranged by size:
808 651 343 109 32 32 32 32
16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 243 495 2055
Size: 243 252 1560
Fragments arranged by size:
1560 252 243
Enzymes that do cut:
AccI AcuI AflII AleI AlfI AloI AlwNI ApoI
AvaI BanI BanII BbsI BbvCI BciVI BclI BglI
BlpI BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MfeI
MmeI MscI MslI MspA1I NcoI NdeI PasI PflMI
PmlI PpiI PpuMI PshAI PstI PvuII SapI SexAI
SfcI SmlI SspI StuI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BfrBI BglII Bme1580I BmrI BmtI BplI
BsaHI BsiEI BsiWI BsmBI BsrGI BssHII BssSI BstBI
BstEII ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MluI NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI