Restriction map for
pFN21AE4559
(Linear) (Six-base) MAPSORT of: KIEE4559.seq Check: 8266 from: 1 to: 2052
KIEE4559.orf
With 182 enzymes: SgfI *
March 22, 2012 18:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 518 1862 2052
Size: 499 19 1344 190
Fragments arranged by size:
1344 499 190 19
ApaI G_GGCC'C
Cuts at: 0 179 198 554 653 688 725 814 994
Size: 179 19 356 99 35 37 89 180
Cuts at: 994 1167 1426 1481 1691 1781 1859 1965 2052
Size: 173 259 55 210 90 78 106 87
Fragments arranged by size:
356 259 210 180 179 173 106 99
90 89 87 78 55 37 35 19
AvaI C'yCGr_G
Cuts at: 0 46 118 180 324 334 420 663 689
Size: 46 72 62 144 10 86 243 26
Cuts at: 689 718 1474 1509 1648 1727 1774 2052
Size: 29 756 35 139 79 47 278
Fragments arranged by size:
756 278 243 144 139 86 79 72
62 47 46 35 29 26 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1734 1767 2052
Size: 1734 33 285
Fragments arranged by size:
1734 285 33
BamHI G'GATC_C
Cuts at: 0 767 2052
Size: 767 1285
BanI G'GyrC_C
Cuts at: 0 292 1297 1432 1450 1644 1882 1951 2052
Size: 292 1005 135 18 194 238 69 101
Fragments arranged by size:
1005 292 238 194 135 101 69 18
BanII G_rGCy'C
Cuts at: 0 179 198 554 653 688 725 814 994
Size: 179 19 356 99 35 37 89 180
Cuts at: 994 1156 1167 1194 1426 1481 1691 1781 1859
Size: 162 11 27 232 55 210 90 78
Cuts at: 1859 1965 2052
Size: 106 87
Fragments arranged by size:
356 232 210 180 179 162 106 99
90 89 87 78 55 37 35 27
19 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 1336 2052
Size: 1336 716
BbvCI CC'TCA_GC
Cuts at: 0 1992 2052
Size: 1992 60
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1591 1625 2052
Size: 1591 34 427
Fragments arranged by size:
1591 427 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1735 2052
Size: 1735 317
BclI T'GATC_A
Cuts at: 0 1595 2052
Size: 1595 457
BglI GCCn_nnn'nGGC
Cuts at: 0 70 101 137 336 413 665 720 926
Size: 70 31 36 199 77 252 55 206
Cuts at: 926 1151 1178 1223 1250 1259 1430 1502 1960
Size: 225 27 45 27 9 171 72 458
Cuts at: 1960 2052
Size: 92
Fragments arranged by size:
458 252 225 206 199 171 92 77
72 70 55 45 36 31 27 27
9
BlpI GC'TnA_GC
Cuts at: 0 1155 2052
Size: 1155 897
Bme1580I G_kGCm'C
Cuts at: 0 179 198 297 523 554 653 688 725
Size: 179 19 99 226 31 99 35 37
Cuts at: 725 814 994 1167 1426 1481 1691 1781 1859
Size: 89 180 173 259 55 210 90 78
Cuts at: 1859 1880 1965 2052
Size: 21 85 87
Fragments arranged by size:
259 226 210 180 179 173 99 99
90 89 87 85 78 55 37 35
31 21 19
BmgBI CAC'GTC
Cuts at: 0 1275 2052
Size: 1275 777
BmrI ACTGGGnnnn_n'
Cuts at: 0 1375 1766 2052
Size: 1375 391 286
Fragments arranged by size:
1375 391 286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 2052
Size: 103 1949
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1109 1127 2052
Size: 146 963 18 925
Fragments arranged by size:
963 925 146 18
Bpu10I CC'TnA_GC
Cuts at: 0 1992 2052
Size: 1992 60
BsaI GGTCTCn'nnnn_
Cuts at: 0 1229 1310 1763 1841 2052
Size: 1229 81 453 78 211
Fragments arranged by size:
1229 453 211 81 78
BsaHI Gr'CG_yC
Cuts at: 0 1100 1505 2052
Size: 1100 405 547
Fragments arranged by size:
1100 547 405
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1817 2052
Size: 1817 235
BseYI C'CCAG_C
Cuts at: 0 226 339 1060 1068 1372 1419 1764 2052
Size: 226 113 721 8 304 47 345 288
Fragments arranged by size:
721 345 304 288 226 113 47 8
BsiEI CG_ry'CG
Cuts at: 0 65 1284 2052
Size: 65 1219 768
Fragments arranged by size:
1219 768 65
BsiHKAI G_wGCw'C
Cuts at: 0 978 1911 2052
Size: 978 933 141
Fragments arranged by size:
978 933 141
BsmI GAATG_Cn'
Cuts at: 0 566 2052
Size: 566 1486
Bsp1286I G_dGCh'C
Cuts at: 0 179 198 297 523 554 653 688 725
Size: 179 19 99 226 31 99 35 37
Cuts at: 725 814 978 994 1156 1167 1194 1426 1481
Size: 89 164 16 162 11 27 232 55
Cuts at: 1481 1691 1781 1859 1880 1911 1965 2052
Size: 210 90 78 21 31 54 87
Fragments arranged by size:
232 226 210 179 164 162 99 99
90 89 87 78 55 54 37 35
31 31 27 21 19 16 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1321 2052
Size: 1321 731
BsrBI CCG'CTC
Cuts at: 0 27 386 1024 2052
Size: 27 359 638 1028
Fragments arranged by size:
1028 638 359 27
BsrFI r'CCGG_y
Cuts at: 0 655 1078 2052
Size: 655 423 974
Fragments arranged by size:
974 655 423
BssHII G'CGCG_C
Cuts at: 0 12 2052
Size: 12 2040
BstEII G'GTnAC_C
Cuts at: 0 631 793 838 1714 2052
Size: 631 162 45 876 338
Fragments arranged by size:
876 631 338 162 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1380 2052
Size: 1380 672
BstYI r'GATC_y
Cuts at: 0 767 1935 1979 2052
Size: 767 1168 44 73
Fragments arranged by size:
1168 767 73 44
Bsu36I CC'TnA_GG
Cuts at: 0 1310 1630 2052
Size: 1310 320 422
Fragments arranged by size:
1310 422 320
BtgI C'CryG_G
Cuts at: 0 411 825 1746 2052
Size: 411 414 921 306
Fragments arranged by size:
921 414 411 306
EaeI y'GGCC_r
Cuts at: 0 2 62 129 878 918 945 1143 1896
Size: 2 60 67 749 40 27 198 753
Cuts at: 1896 2052
Size: 156
Fragments arranged by size:
753 749 198 156 67 60 40 27
2
EagI C'GGCC_G
Cuts at: 0 62 2052
Size: 62 1990
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 499 518 1109 1127 1862 2052
Size: 146 353 19 591 18 735 190
Fragments arranged by size:
735 591 353 190 146 19 18
EcoO109I rG'GnC_Cy
Cuts at: 0 176 194 195 319 424 460 541 550
Size: 176 18 1 124 105 36 81 9
Cuts at: 550 551 577 684 685 722 739 810 990
Size: 1 26 107 1 37 17 71 180
Cuts at: 990 991 1163 1164 1180 1252 1260 1306 1369
Size: 1 172 1 16 72 8 46 63
Cuts at: 1369 1423 1441 1478 1688 1696 1732 1855 1856
Size: 54 18 37 210 8 36 123 1
Cuts at: 1856 1864 1961 2052
Size: 8 97 91
Fragments arranged by size:
210 180 176 172 124 123 107 105
97 91 81 72 71 63 54 46
37 37 36 36 26 18 18 17
16 9 8 8 8 1 1 1
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1655 2052
Size: 1655 397
FseI GG_CCGG'CC
Cuts at: 0 1082 2052
Size: 1082 970
HaeII r_GCGC'y
Cuts at: 0 506 781 1727 2052
Size: 506 275 946 325
Fragments arranged by size:
946 506 325 275
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1361 1365 2052
Size: 1361 4 687
Fragments arranged by size:
1361 687 4
MscI TGG'CCA
Cuts at: 0 947 1145 2052
Size: 947 198 907
Fragments arranged by size:
947 907 198
MspA1I CmG'CkG
Cuts at: 0 413 827 1159 1376 1457 1675 1748 2052
Size: 413 414 332 217 81 218 73 304
Fragments arranged by size:
414 413 332 304 218 217 81 73
NaeI GCC'GGC
Cuts at: 0 657 1080 2052
Size: 657 423 972
Fragments arranged by size:
972 657 423
NgoMIV G'CCGG_C
Cuts at: 0 655 1078 2052
Size: 655 423 974
Fragments arranged by size:
974 655 423
NotI GC'GGCC_GC
Cuts at: 0 62 2052
Size: 62 1990
NspI r_CATG'y
Cuts at: 0 906 1122 2052
Size: 906 216 930
Fragments arranged by size:
930 906 216
PasI CC'CwG_GG
Cuts at: 0 109 298 547 646 727 1257 1437 1684
Size: 109 189 249 99 81 530 180 247
Cuts at: 1684 1965 2052
Size: 281 87
Fragments arranged by size:
530 281 249 247 189 180 109 99
87 81
PfoI T'CCnGG_A
Cuts at: 0 465 978 1185 1290 1929 2052
Size: 465 513 207 105 639 123
Fragments arranged by size:
639 513 465 207 123 105
PpuMI rG'GwC_Cy
Cuts at: 0 319 424 460 541 577 739 1369 1441
Size: 319 105 36 81 36 162 630 72
Cuts at: 1441 1696 1732 1864 2052
Size: 255 36 132 188
Fragments arranged by size:
630 319 255 188 162 132 105 81
72 36 36 36
PshAI GACnn'nnGTC
Cuts at: 0 737 1286 2052
Size: 737 549 766
Fragments arranged by size:
766 737 549
PspOMI G'GGCC_C
Cuts at: 0 175 194 550 649 684 721 810 990
Size: 175 19 356 99 35 37 89 180
Cuts at: 990 1163 1422 1477 1687 1777 1855 1961 2052
Size: 173 259 55 210 90 78 106 91
Fragments arranged by size:
356 259 210 180 175 173 106 99
91 90 89 78 55 37 35 19
PspXI vC'TCGA_Gb
Cuts at: 0 1727 2052
Size: 1727 325
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1317 1349 2052
Size: 1317 32 703
Fragments arranged by size:
1317 703 32
PstI C_TGCA'G
Cuts at: 0 683 1502 2052
Size: 683 819 550
Fragments arranged by size:
819 683 550
PvuII CAG'CTG
Cuts at: 0 1675 2052
Size: 1675 377
SacII CC_GC'GG
Cuts at: 0 414 828 1749 2052
Size: 414 414 921 303
Fragments arranged by size:
921 414 414 303
SanDI GG'GwC_CC
Cuts at: 0 319 577 739 1369 1441 2052
Size: 319 258 162 630 72 611
Fragments arranged by size:
630 611 319 258 162 72
SbfI CC_TGCA'GG
Cuts at: 0 1502 2052
Size: 1502 550
SexAI A'CCwGG_T
Cuts at: 0 852 2052
Size: 852 1200
SfcI C'TryA_G
Cuts at: 0 679 1498 2052
Size: 679 819 554
Fragments arranged by size:
819 679 554
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 101 413 720 1178 1250 1259 2052
Size: 101 312 307 458 72 9 793
Fragments arranged by size:
793 458 312 307 101 72 9
SmaI CCC'GGG
Cuts at: 0 120 182 326 336 422 665 691 1476
Size: 120 62 144 10 86 243 26 785
Cuts at: 1476 1511 1650 1776 2052
Size: 35 139 126 276
Fragments arranged by size:
785 276 243 144 139 126 120 86
62 35 26 10
SmlI C'TyrA_G
Cuts at: 0 1727 2052
Size: 1727 325
SphI G_CATG'C
Cuts at: 0 906 1122 2052
Size: 906 216 930
Fragments arranged by size:
930 906 216
SrfI GCCC'GGGC
Cuts at: 0 336 2052
Size: 336 1716
StuI AGG'CCT
Cuts at: 0 353 2052
Size: 353 1699
StyI C'CwwG_G
Cuts at: 0 287 833 860 1860 1869 2001 2052
Size: 287 546 27 1000 9 132 51
Fragments arranged by size:
1000 546 287 132 51 27 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 497 812 1060 1450 2052
Size: 497 315 248 390 602
Fragments arranged by size:
602 497 390 315 248
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 777 809 1425 1457 2052
Size: 777 32 616 32 595
Fragments arranged by size:
777 616 595 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 854 2052
Size: 854 1198
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1761 2052
Size: 1761 291
XhoI C'TCGA_G
Cuts at: 0 1727 2052
Size: 1727 325
XmaI C'CCGG_G
Cuts at: 0 118 180 324 334 420 663 689 1474
Size: 118 62 144 10 86 243 26 785
Cuts at: 1474 1509 1648 1774 2052
Size: 35 139 126 278
Fragments arranged by size:
785 278 243 144 139 126 118 86
62 35 26 10
Enzymes that do cut:
AcuI ApaI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaHI BseRI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII
BstEII BstXI BstYI Bsu36I BtgI EaeI EagI Eco57MI
EcoO109I FalI FseI HaeII MmeI MscI MspA1I NaeI
NgoMIV NotI NspI PasI PfoI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuII SacII SanDI SbfI SexAI
SfcI SfiI SmaI SmlI SphI SrfI StuI StyI
TaqII TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvrII BbeI BfrBI BglII BmtI
BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiWI BsmBI
BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
NarI NcoI NdeI NheI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PsiI PvuI RsrII SacI
SalI SapI ScaI SfoI SgfI SgrAI SnaBI SpeI
SspI SwaI TatI XbaI XmnI ZraI