Restriction map for
pFN21AE4557
(Linear) (Six-base) MAPSORT of: KIEE4557.seq Check: 7259 from: 1 to: 2037
KIEE4557.orf
With 182 enzymes: SgfI *
March 22, 2012 18:22 ..
AccI GT'mk_AC
Cuts at: 0 643 2037
Size: 643 1394
Acc65I G'GTAC_C
Cuts at: 0 688 2037
Size: 688 1349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1547 1782 1998 2037
Size: 196 1351 235 216 39
Fragments arranged by size:
1351 235 216 196 39
AflII C'TTAA_G
Cuts at: 0 1233 2037
Size: 1233 804
AleI CACnn'nnGTG
Cuts at: 0 1828 2037
Size: 1828 209
AlwNI CAG_nnn'CTG
Cuts at: 0 1400 1432 1551 2037
Size: 1400 32 119 486
Fragments arranged by size:
1400 486 119 32
ApoI r'AATT_y
Cuts at: 0 135 521 1373 2037
Size: 135 386 852 664
Fragments arranged by size:
852 664 386 135
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 137 170 2037
Size: 137 33 1867
Fragments arranged by size:
1867 137 33
BanI G'GyrC_C
Cuts at: 0 688 2037
Size: 688 1349
BanII G_rGCy'C
Cuts at: 0 160 220 1885 2037
Size: 160 60 1665 152
Fragments arranged by size:
1665 160 152 60
BbsI GAAGACnn'nnnn_
Cuts at: 0 845 2037
Size: 845 1192
BglI GCCn_nnn'nGGC
Cuts at: 0 2005 2037
Size: 2005 32
Bme1580I G_kGCm'C
Cuts at: 0 729 920 2037
Size: 729 191 1117
Fragments arranged by size:
1117 729 191
BmgBI CAC'GTC
Cuts at: 0 273 2037
Size: 273 1764
BmrI ACTGGGnnnn_n'
Cuts at: 0 238 2037
Size: 238 1799
BmtI G_CTAG'C
Cuts at: 0 200 2037
Size: 200 1837
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 907 965 1098 2003 2037
Size: 668 239 58 133 905 34
Fragments arranged by size:
905 668 239 133 58 34
Bpu10I CC'TnA_GC
Cuts at: 0 159 2037
Size: 159 1878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 2037
Size: 278 1759
BsaBI GATnn'nnATC
Cuts at: 0 73 2037
Size: 73 1964
BsaHI Gr'CG_yC
Cuts at: 0 1932 2037
Size: 1932 105
BsaWI w'CCGG_w
Cuts at: 0 254 1219 1907 2037
Size: 254 965 688 130
Fragments arranged by size:
965 688 254 130
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 952 1528 2037
Size: 952 576 509
Fragments arranged by size:
952 576 509
BseYI C'CCAG_C
Cuts at: 0 660 1000 2037
Size: 660 340 1037
Fragments arranged by size:
1037 660 340
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 734 1070 1075 2037
Size: 594 140 336 5 962
Fragments arranged by size:
962 594 336 140 5
BsiHKAI G_wGCw'C
Cuts at: 0 654 2037
Size: 654 1383
BsmI GAATG_Cn'
Cuts at: 0 106 544 2037
Size: 106 438 1493
Fragments arranged by size:
1493 438 106
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1939 2037
Size: 1939 98
Bsp1286I G_dGCh'C
Cuts at: 0 160 220 654 729 920 1885 2037
Size: 160 60 434 75 191 965 152
Fragments arranged by size:
965 434 191 160 152 75 60
BspEI T'CCGG_A
Cuts at: 0 254 2037
Size: 254 1783
BsrDI GCAATG_nn'
Cuts at: 0 1410 1602 1614 2037
Size: 1410 192 12 423
Fragments arranged by size:
1410 423 192 12
BsrFI r'CCGG_y
Cuts at: 0 310 2037
Size: 310 1727
BsrGI T'GTAC_A
Cuts at: 0 281 1160 2037
Size: 281 879 877
Fragments arranged by size:
879 877 281
BssSI C'ACGA_G
Cuts at: 0 352 424 2037
Size: 352 72 1613
Fragments arranged by size:
1613 352 72
BstBI TT'CG_AA
Cuts at: 0 1377 2037
Size: 1377 660
BstYI r'GATC_y
Cuts at: 0 564 1556 1767 2037
Size: 564 992 211 270
Fragments arranged by size:
992 564 270 211
BstZ17I GTA'TAC
Cuts at: 0 644 2037
Size: 644 1393
BtgI C'CryG_G
Cuts at: 0 113 1265 1587 1757 2037
Size: 113 1152 322 170 280
Fragments arranged by size:
1152 322 280 170 113
BtsI GCAGTG_nn'
Cuts at: 0 496 1058 2037
Size: 496 562 979
Fragments arranged by size:
979 562 496
DraIII CAC_nnn'GTG
Cuts at: 0 1140 2037
Size: 1140 897
DrdI GACnn_nn'nnGTC
Cuts at: 0 1447 2037
Size: 1447 590
EaeI y'GGCC_r
Cuts at: 0 308 1119 2037
Size: 308 811 918
Fragments arranged by size:
918 811 308
EarI CTCTTCn'nnn_
Cuts at: 0 757 1546 1737 2037
Size: 757 789 191 300
Fragments arranged by size:
789 757 300 191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 668 907 965 1098 1547 1782 1998
Size: 196 472 239 58 133 449 235 216
Cuts at: 1998 2003 2037
Size: 5 34
Fragments arranged by size:
472 449 239 235 216 196 133 58
34 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 556 2037
Size: 556 1481
EcoO109I rG'GnC_Cy
Cuts at: 0 904 2037
Size: 904 1133
EcoRI G'AATT_C
Cuts at: 0 1373 2037
Size: 1373 664
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 846 2037
Size: 846 1191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1408 1440 2009 2037
Size: 1408 32 569 28
Fragments arranged by size:
1408 569 32 28
HincII GTy'rAC
Cuts at: 0 1926 2037
Size: 1926 111
KpnI G_GTAC'C
Cuts at: 0 692 2037
Size: 692 1345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 597 2037
Size: 597 1440
MscI TGG'CCA
Cuts at: 0 1121 2037
Size: 1121 916
MslI CAynn'nnrTG
Cuts at: 0 703 1683 1828 1951 2037
Size: 703 980 145 123 86
Fragments arranged by size:
980 703 145 123 86
MspA1I CmG'CkG
Cuts at: 0 28 151 2037
Size: 28 123 1886
Fragments arranged by size:
1886 123 28
NaeI GCC'GGC
Cuts at: 0 312 2037
Size: 312 1725
NcoI C'CATG_G
Cuts at: 0 1265 1587 1757 2037
Size: 1265 322 170 280
Fragments arranged by size:
1265 322 280 170
NgoMIV G'CCGG_C
Cuts at: 0 310 2037
Size: 310 1727
NheI G'CTAG_C
Cuts at: 0 196 2037
Size: 196 1841
NspI r_CATG'y
Cuts at: 0 1284 2037
Size: 1284 753
PflMI CCAn_nnn'nTGG
Cuts at: 0 930 1758 2037
Size: 930 828 279
Fragments arranged by size:
930 828 279
PshAI GACnn'nnGTC
Cuts at: 0 1931 2037
Size: 1931 106
PstI C_TGCA'G
Cuts at: 0 1556 2037
Size: 1556 481
PvuII CAG'CTG
Cuts at: 0 28 151 2037
Size: 28 123 1886
Fragments arranged by size:
1886 123 28
ScaI AGT'ACT
Cuts at: 0 21 2037
Size: 21 2016
SfcI C'TryA_G
Cuts at: 0 23 1552 2037
Size: 23 1529 485
Fragments arranged by size:
1529 485 23
SmlI C'TyrA_G
Cuts at: 0 293 1233 2037
Size: 293 940 804
Fragments arranged by size:
940 804 293
SphI G_CATG'C
Cuts at: 0 1284 2037
Size: 1284 753
SspI AAT'ATT
Cuts at: 0 1845 2037
Size: 1845 192
StyI C'CwwG_G
Cuts at: 0 1193 1265 1587 1757 2037
Size: 1193 72 322 170 280
Fragments arranged by size:
1193 322 280 170 72
TatI w'GTAC_w
Cuts at: 0 19 281 1160 1861 2037
Size: 19 262 879 701 176
Fragments arranged by size:
879 701 262 176 19
XbaI T'CTAG_A
Cuts at: 0 1497 2037
Size: 1497 540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 397 1620 2037
Size: 397 1223 417
Fragments arranged by size:
1223 417 397
Enzymes that do cut:
AccI Acc65I AcuI AflII AleI AlwNI ApoI BaeI
BanI BanII BbsI BglI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrDI BsrFI
BsrGI BssSI BstBI BstYI BstZ17I BtgI BtsI DraIII
DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NheI NspI PflMI PshAI PstI PvuII
ScaI SfcI SmlI SphI SspI StyI TatI XbaI
XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BplI BsaI BsaAI BsaXI BsiEI BsiWI BspHI
BspMI BsrBI BssHII BstAPI BstEII BstXI Bsu36I BtgZI
ClaI CspCI DraI EagI EciI EcoICRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
NarI NdeI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TaqII TstI Tth111I XhoI
XmaI XmnI ZraI