Restriction map for
pFN21AE4551
(Linear) (Six-base) MAPSORT of: KIEE4551.seq Check: 5008 from: 1 to: 1983
KIEE4551.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1044 1212 1983
Size: 1044 168 771
Fragments arranged by size:
1044 771 168
AccI GT'mk_AC
Cuts at: 0 563 1336 1917 1983
Size: 563 773 581 66
Fragments arranged by size:
773 581 563 66
Acc65I G'GTAC_C
Cuts at: 0 1156 1983
Size: 1156 827
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1088 1328 1693 1983
Size: 776 312 240 365 290
Fragments arranged by size:
776 365 312 290 240
AflII C'TTAA_G
Cuts at: 0 1389 1983
Size: 1389 594
AleI CACnn'nnGTG
Cuts at: 0 1048 1983
Size: 1048 935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 547 1983
Size: 547 1436
AlwNI CAG_nnn'CTG
Cuts at: 0 502 711 859 1617 1671 1907 1983
Size: 502 209 148 758 54 236 76
Fragments arranged by size:
758 502 236 209 148 76 54
ApaI G_GGCC'C
Cuts at: 0 496 1366 1983
Size: 496 870 617
Fragments arranged by size:
870 617 496
AvaI C'yCGr_G
Cuts at: 0 812 1683 1983
Size: 812 871 300
Fragments arranged by size:
871 812 300
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 59 92 1983
Size: 59 33 1891
Fragments arranged by size:
1891 59 33
BamHI G'GATC_C
Cuts at: 0 577 1983
Size: 577 1406
BanI G'GyrC_C
Cuts at: 0 636 922 1017 1056 1134 1156 1595 1983
Size: 636 286 95 39 78 22 439 388
Fragments arranged by size:
636 439 388 286 95 78 39 22
BanII G_rGCy'C
Cuts at: 0 496 1034 1366 1983
Size: 496 538 332 617
Fragments arranged by size:
617 538 496 332
BbeI G_GCGC'C
Cuts at: 0 1021 1983
Size: 1021 962
BbsI GAAGACnn'nnnn_
Cuts at: 0 466 1983
Size: 466 1517
BciVI GTATCCnnnnn_n'
Cuts at: 0 155 374 1983
Size: 155 219 1609
Fragments arranged by size:
1609 219 155
BfrBI ATG'CAT
Cuts at: 0 1525 1983
Size: 1525 458
BglI GCCn_nnn'nGGC
Cuts at: 0 297 419 1983
Size: 297 122 1564
Fragments arranged by size:
1564 297 122
BglII A'GATC_T
Cuts at: 0 1580 1983
Size: 1580 403
BlpI GC'TnA_GC
Cuts at: 0 508 612 1983
Size: 508 104 1371
Fragments arranged by size:
1371 508 104
Bme1580I G_kGCm'C
Cuts at: 0 496 541 819 938 1366 1983
Size: 496 45 278 119 428 617
Fragments arranged by size:
617 496 428 278 119 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 490 654 847 907 1878 1983
Size: 490 164 193 60 971 105
Fragments arranged by size:
971 490 193 164 105 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 709 862 1166 1983
Size: 201 508 153 304 817
Fragments arranged by size:
817 508 304 201 153
Bpu10I CC'TnA_GC
Cuts at: 0 1301 1983
Size: 1301 682
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 187 1983
Size: 90 97 1796
Fragments arranged by size:
1796 97 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 434 1267 1983
Size: 434 833 716
Fragments arranged by size:
833 716 434
BsaHI Gr'CG_yC
Cuts at: 0 1018 1983
Size: 1018 965
BsaWI w'CCGG_w
Cuts at: 0 1510 1983
Size: 1510 473
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 174 204 1983
Size: 174 30 1779
Fragments arranged by size:
1779 174 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1954 1983
Size: 1954 29
BseYI C'CCAG_C
Cuts at: 0 534 1610 1636 1641 1745 1983
Size: 534 1076 26 5 104 238
Fragments arranged by size:
1076 534 238 104 26 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1067 1072 1111 1229 1645 1983
Size: 1067 5 39 118 416 338
Fragments arranged by size:
1067 416 338 118 39 5
BsiHKAI G_wGCw'C
Cuts at: 0 1114 1983
Size: 1114 869
Bsp1286I G_dGCh'C
Cuts at: 0 496 541 819 938 1034 1114 1366 1983
Size: 496 45 278 119 96 80 252 617
Fragments arranged by size:
617 496 278 252 119 96 80 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 291 1044 1212 1773 1968 1983
Size: 291 753 168 561 195 15
Fragments arranged by size:
753 561 291 195 168 15
BsrDI GCAATG_nn'
Cuts at: 0 536 1983
Size: 536 1447
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1503 1931 1983
Size: 1503 428 52
Fragments arranged by size:
1503 428 52
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1728 1983
Size: 1728 255
BstYI r'GATC_y
Cuts at: 0 3 577 1490 1580 1983
Size: 3 574 913 90 403
Fragments arranged by size:
913 574 403 90 3
BtgI C'CryG_G
Cuts at: 0 548 1983
Size: 548 1435
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 553 1571 1983
Size: 553 1018 412
Fragments arranged by size:
1018 553 412
BtsI GCAGTG_nn'
Cuts at: 0 857 1079 1739 1983
Size: 857 222 660 244
Fragments arranged by size:
857 660 244 222
ClaI AT'CG_AT
Cuts at: 0 1434 1983
Size: 1434 549
EaeI y'GGCC_r
Cuts at: 0 972 1983
Size: 972 1011
EarI CTCTTCn'nnn_
Cuts at: 0 430 1396 1983
Size: 430 966 587
Fragments arranged by size:
966 587 430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 709 776 862 1088 1166 1328 1693
Size: 201 508 67 86 226 78 162 365
Cuts at: 1693 1983
Size: 290
Fragments arranged by size:
508 365 290 226 201 162 86 78
67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 132 1780 1983
Size: 132 1648 203
Fragments arranged by size:
1648 203 132
EcoO109I rG'GnC_Cy
Cuts at: 0 492 954 1363 1983
Size: 492 462 409 620
Fragments arranged by size:
620 492 462 409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 459 1792 1887 1983
Size: 459 1333 95 96
Fragments arranged by size:
1333 459 96 95
HaeII r_GCGC'y
Cuts at: 0 1021 1983
Size: 1021 962
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 174 206 1164 1196 1309 1341 1570 1602
Size: 174 32 958 32 113 32 229 32
Cuts at: 1602 1983
Size: 381
Fragments arranged by size:
958 381 229 174 113 32 32 32
32
HincII GTy'rAC
Cuts at: 0 315 1178 1983
Size: 315 863 805
Fragments arranged by size:
863 805 315
HindIII A'AGCT_T
Cuts at: 0 441 1983
Size: 441 1542
KasI G'GCGC_C
Cuts at: 0 1017 1983
Size: 1017 966
KpnI G_GTAC'C
Cuts at: 0 1160 1983
Size: 1160 823
MfeI C'AATT_G
Cuts at: 0 1217 1983
Size: 1217 766
MscI TGG'CCA
Cuts at: 0 974 1983
Size: 974 1009
MslI CAynn'nnrTG
Cuts at: 0 553 1048 1117 1983
Size: 553 495 69 866
Fragments arranged by size:
866 553 495 69
MspA1I CmG'CkG
Cuts at: 0 141 970 1243 1614 1653 1791 1983
Size: 141 829 273 371 39 138 192
Fragments arranged by size:
829 371 273 192 141 138 39
NarI GG'CG_CC
Cuts at: 0 1018 1983
Size: 1018 965
NcoI C'CATG_G
Cuts at: 0 548 1983
Size: 548 1435
NsiI A_TGCA'T
Cuts at: 0 1527 1983
Size: 1527 456
PasI CC'CwG_GG
Cuts at: 0 1359 1983
Size: 1359 624
PflMI CCAn_nnn'nTGG
Cuts at: 0 128 502 1983
Size: 128 374 1481
Fragments arranged by size:
1481 374 128
PfoI T'CCnGG_A
Cuts at: 0 580 1983
Size: 580 1403
PspOMI G'GGCC_C
Cuts at: 0 492 1362 1983
Size: 492 870 621
Fragments arranged by size:
870 621 492
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1338 1370 1983
Size: 1338 32 613
Fragments arranged by size:
1338 613 32
PstI C_TGCA'G
Cuts at: 0 878 951 1029 1107 1209 1499 1983
Size: 878 73 78 78 102 290 484
Fragments arranged by size:
878 484 290 102 78 78 73
PvuII CAG'CTG
Cuts at: 0 141 970 1243 1653 1983
Size: 141 829 273 410 330
Fragments arranged by size:
829 410 330 273 141
SbfI CC_TGCA'GG
Cuts at: 0 878 1499 1983
Size: 878 621 484
Fragments arranged by size:
878 621 484
SexAI A'CCwGG_T
Cuts at: 0 632 1008 1983
Size: 632 376 975
Fragments arranged by size:
975 632 376
SfcI C'TryA_G
Cuts at: 0 874 947 986 1025 1103 1190 1205 1259
Size: 874 73 39 39 78 87 15 54
Cuts at: 1259 1495 1983
Size: 236 488
Fragments arranged by size:
874 488 236 87 78 73 54 39
39 15
SfoI GGC'GCC
Cuts at: 0 1019 1983
Size: 1019 964
SmlI C'TyrA_G
Cuts at: 0 105 202 1389 1983
Size: 105 97 1187 594
Fragments arranged by size:
1187 594 105 97
StuI AGG'CCT
Cuts at: 0 1775 1983
Size: 1775 208
StyI C'CwwG_G
Cuts at: 0 548 671 1548 1562 1776 1983
Size: 548 123 877 14 214 207
Fragments arranged by size:
877 548 214 207 123 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1257 1759 1983
Size: 1257 502 224
Fragments arranged by size:
1257 502 224
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 344 376 1983
Size: 344 32 1607
Fragments arranged by size:
1607 344 32
Tth111I GACn'n_nGTC
Cuts at: 0 1465 1539 1983
Size: 1465 74 444
Fragments arranged by size:
1465 444 74
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 94 422 739 1392 1983
Size: 94 328 317 653 591
Fragments arranged by size:
653 591 328 317 94
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AleI AlfI AlwNI
ApaI AvaI BaeI BamHI BanI BanII BbeI BbsI
BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BstAPI BstXI BstYI
BtgI BtgZI BtsI ClaI EaeI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MscI MslI MspA1I NarI NcoI NsiI PasI
PflMI PfoI PspOMI PsrI PstI PvuII SbfI SexAI
SfcI SfoI SmlI StuI StyI TaqII TstI Tth111I
XcmI
Enzymes that do not cut:
AatII AclI AfeI AflIII AgeI AhdI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BbvCI BcgI BclI
BmgBI BmtI BplI BsaAI BsaBI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HpaI MluI MmeI NaeI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI XbaI XhoI
XmaI XmnI ZraI