Restriction map for
pFN21AE4548
(Linear) (Six-base) MAPSORT of: KIEE4548.seq Check: 2463 from: 1 to: 1941
KIEE4548.orf
With 182 enzymes: SgfI *
March 22, 2012 18:20 ..
Acc65I G'GTAC_C
Cuts at: 0 81 457 1941
Size: 81 376 1484
Fragments arranged by size:
1484 376 81
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 854 1004 1433 1760 1941
Size: 473 381 150 429 327 181
Fragments arranged by size:
473 429 381 327 181 150
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 174 206 1941
Size: 174 32 1735
Fragments arranged by size:
1735 174 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1228 1941
Size: 1228 713
ApoI r'AATT_y
Cuts at: 0 1418 1482 1669 1722 1941
Size: 1418 64 187 53 219
Fragments arranged by size:
1418 219 187 64 53
AseI AT'TA_AT
Cuts at: 0 1306 1941
Size: 1306 635
AvaI C'yCGr_G
Cuts at: 0 52 1941
Size: 52 1889
BanI G'GyrC_C
Cuts at: 0 81 316 457 1236 1941
Size: 81 235 141 779 705
Fragments arranged by size:
779 705 235 141 81
BanII G_rGCy'C
Cuts at: 0 27 1887 1941
Size: 27 1860 54
Fragments arranged by size:
1860 54 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 1248 1941
Size: 253 995 693
Fragments arranged by size:
995 693 253
BbvCI CC'TCA_GC
Cuts at: 0 549 1941
Size: 549 1392
BclI T'GATC_A
Cuts at: 0 1854 1941
Size: 1854 87
BglI GCCn_nnn'nGGC
Cuts at: 0 43 1941
Size: 43 1898
Bme1580I G_kGCm'C
Cuts at: 0 171 700 1941
Size: 171 529 1241
Fragments arranged by size:
1241 529 171
BmrI ACTGGGnnnn_n'
Cuts at: 0 182 1881 1941
Size: 182 1699 60
Fragments arranged by size:
1699 182 60
BmtI G_CTAG'C
Cuts at: 0 1829 1941
Size: 1829 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1941
Size: 439 1502
Bpu10I CC'TnA_GC
Cuts at: 0 549 623 1941
Size: 549 74 1318
Fragments arranged by size:
1318 549 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1733 1941
Size: 1733 208
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 1941
Size: 436 1505
BseYI C'CCAG_C
Cuts at: 0 576 998 1032 1941
Size: 576 422 34 909
Fragments arranged by size:
909 576 422 34
BsiEI CG_ry'CG
Cuts at: 0 246 1941
Size: 246 1695
Bsp1286I G_dGCh'C
Cuts at: 0 27 171 700 1887 1941
Size: 27 144 529 1187 54
Fragments arranged by size:
1187 529 144 54 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 648 1941
Size: 648 1293
BsrBI CCG'CTC
Cuts at: 0 127 1941
Size: 127 1814
BsrDI GCAATG_nn'
Cuts at: 0 283 1607 1941
Size: 283 1324 334
Fragments arranged by size:
1324 334 283
BssSI C'ACGA_G
Cuts at: 0 95 1941
Size: 95 1846
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1771 1941
Size: 1771 170
BstEII G'GTnAC_C
Cuts at: 0 364 1941
Size: 364 1577
BstXI CCAn_nnnn'nTGG
Cuts at: 0 693 1941
Size: 693 1248
Bsu36I CC'TnA_GG
Cuts at: 0 509 1941
Size: 509 1432
BtgI C'CryG_G
Cuts at: 0 40 205 692 1941
Size: 40 165 487 1249
Fragments arranged by size:
1249 487 165 40
BtsI GCAGTG_nn'
Cuts at: 0 596 1941
Size: 596 1345
ClaI AT'CG_AT
Cuts at: 0 1349 1941
Size: 1349 592
DraI TTT'AAA
Cuts at: 0 528 941 1466 1941
Size: 528 413 525 475
Fragments arranged by size:
528 525 475 413
DraIII CAC_nnn'GTG
Cuts at: 0 1863 1941
Size: 1863 78
EaeI y'GGCC_r
Cuts at: 0 243 977 1941
Size: 243 734 964
Fragments arranged by size:
964 734 243
EagI C'GGCC_G
Cuts at: 0 243 1941
Size: 243 1698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 473 854 1004 1433 1760 1941
Size: 439 34 381 150 429 327 181
Fragments arranged by size:
439 429 381 327 181 150 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1220 1941
Size: 1220 721
EcoO109I rG'GnC_Cy
Cuts at: 0 1225 1941
Size: 1225 716
EcoRI G'AATT_C
Cuts at: 0 1669 1941
Size: 1669 272
EcoRV GAT'ATC
Cuts at: 0 1164 1266 1941
Size: 1164 102 675
Fragments arranged by size:
1164 675 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 470 502 1153 1185 1941
Size: 470 32 651 32 756
Fragments arranged by size:
756 651 470 32 32
HindIII A'AGCT_T
Cuts at: 0 1445 1941
Size: 1445 496
KpnI G_GTAC'C
Cuts at: 0 85 461 1941
Size: 85 376 1480
Fragments arranged by size:
1480 376 85
MfeI C'AATT_G
Cuts at: 0 1766 1941
Size: 1766 175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1015 1860 1941
Size: 384 631 845 81
Fragments arranged by size:
845 631 384 81
MslI CAynn'nnrTG
Cuts at: 0 691 1941
Size: 691 1250
MspA1I CmG'CkG
Cuts at: 0 207 982 1207 1941
Size: 207 775 225 734
Fragments arranged by size:
775 734 225 207
NcoI C'CATG_G
Cuts at: 0 40 692 1941
Size: 40 652 1249
Fragments arranged by size:
1249 652 40
NheI G'CTAG_C
Cuts at: 0 1825 1941
Size: 1825 116
NruI TCG'CGA
Cuts at: 0 108 1941
Size: 108 1833
NspI r_CATG'y
Cuts at: 0 657 1463 1757 1941
Size: 657 806 294 184
Fragments arranged by size:
806 657 294 184
PfoI T'CCnGG_A
Cuts at: 0 1227 1941
Size: 1227 714
PpuMI rG'GwC_Cy
Cuts at: 0 1225 1941
Size: 1225 716
PsiI TTA'TAA
Cuts at: 0 1507 1941
Size: 1507 434
PspXI vC'TCGA_Gb
Cuts at: 0 52 1941
Size: 52 1889
PstI C_TGCA'G
Cuts at: 0 227 1557 1800 1941
Size: 227 1330 243 141
Fragments arranged by size:
1330 243 227 141
PvuII CAG'CTG
Cuts at: 0 1207 1941
Size: 1207 734
SacII CC_GC'GG
Cuts at: 0 208 1941
Size: 208 1733
SbfI CC_TGCA'GG
Cuts at: 0 1557 1941
Size: 1557 384
ScaI AGT'ACT
Cuts at: 0 1548 1941
Size: 1548 393
SfcI C'TryA_G
Cuts at: 0 146 223 782 915 1247 1553 1657 1796
Size: 146 77 559 133 332 306 104 139
Cuts at: 1796 1941
Size: 145
Fragments arranged by size:
559 332 306 146 145 139 133 104
77
SmlI C'TyrA_G
Cuts at: 0 52 1748 1941
Size: 52 1696 193
Fragments arranged by size:
1696 193 52
StuI AGG'CCT
Cuts at: 0 523 1941
Size: 523 1418
StyI C'CwwG_G
Cuts at: 0 40 692 1941
Size: 40 652 1249
Fragments arranged by size:
1249 652 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 715 1941
Size: 715 1226
TatI w'GTAC_w
Cuts at: 0 795 1546 1941
Size: 795 751 395
Fragments arranged by size:
795 751 395
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1655 1687 1941
Size: 1655 32 254
Fragments arranged by size:
1655 254 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 690 1941
Size: 690 1251
XhoI C'TCGA_G
Cuts at: 0 52 1941
Size: 52 1889
Enzymes that do cut:
Acc65I AcuI AloI AlwNI ApoI AseI AvaI BanI
BanII BbsI BbvCI BclI BglI Bme1580I BmrI BmtI
BpmI Bpu10I BpuEI BseRI BseYI BsiEI Bsp1286I BspMI
BsrBI BsrDI BssSI BstAPI BstEII BstXI Bsu36I BtgI
BtsI ClaI DraI DraIII EaeI EagI Eco57MI EcoNI
EcoO109I EcoRI EcoRV Hin4I HindIII KpnI MfeI MmeI
MslI MspA1I NcoI NheI NruI NspI PfoI PpuMI
PsiI PspXI PstI PvuII SacII SbfI ScaI SfcI
SmlI StuI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BfrBI BglII BlpI
BmgBI BplI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrFI
BsrGI BssHII BstBI BstYI BstZ17I BtgZI CspCI DrdI
EarI EciI EcoICRI FalI FseI FspI FspAI HaeII
HincII HpaI KasI MluI MscI NaeI NarI NdeI
NgoMIV NotI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PspOMI PsrI PvuI RsrII SacI
SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XmaI XmnI ZraI