Restriction map for
pFN21AE4547
(Linear) (Six-base) MAPSORT of: KIEE4547.seq Check: 1775 from: 1 to: 1929
KIEE4547.orf
With 182 enzymes: SgfI *
March 22, 2012 18:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1213 1929
Size: 1213 716
AatII G_ACGT'C
Cuts at: 0 1007 1929
Size: 1007 922
AflIII A'CryG_T
Cuts at: 0 509 887 1929
Size: 509 378 1042
Fragments arranged by size:
1042 509 378
AleI CACnn'nnGTG
Cuts at: 0 250 1929
Size: 250 1679
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 328 360 1929
Size: 328 32 1569
Fragments arranged by size:
1569 328 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1477 1929
Size: 1477 452
ApoI r'AATT_y
Cuts at: 0 736 1929
Size: 736 1193
AvaI C'yCGr_G
Cuts at: 0 352 1929
Size: 352 1577
BanI G'GyrC_C
Cuts at: 0 40 613 1929
Size: 40 573 1316
Fragments arranged by size:
1316 573 40
BanII G_rGCy'C
Cuts at: 0 20 506 533 1024 1929
Size: 20 486 27 491 905
Fragments arranged by size:
905 491 486 27 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 1311 1635 1929
Size: 1311 324 294
Fragments arranged by size:
1311 324 294
BbvCI CC'TCA_GC
Cuts at: 0 1586 1929
Size: 1586 343
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1795 1829 1929
Size: 1795 34 100
Fragments arranged by size:
1795 100 34
BclI T'GATC_A
Cuts at: 0 434 1929
Size: 434 1495
BglI GCCn_nnn'nGGC
Cuts at: 0 1120 1929
Size: 1120 809
BglII A'GATC_T
Cuts at: 0 359 1929
Size: 359 1570
BlpI GC'TnA_GC
Cuts at: 0 376 1534 1929
Size: 376 1158 395
Fragments arranged by size:
1158 395 376
Bme1580I G_kGCm'C
Cuts at: 0 43 446 616 783 906 1929
Size: 43 403 170 167 123 1023
Fragments arranged by size:
1023 403 170 167 123 43
BmgBI CAC'GTC
Cuts at: 0 890 1929
Size: 890 1039
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 378 876 1929
Size: 378 498 1053
Fragments arranged by size:
1053 498 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 816 871 967 1234 1651 1768 1812
Size: 695 121 55 96 267 417 117 44
Cuts at: 1812 1822 1929
Size: 10 107
Fragments arranged by size:
695 417 267 121 117 107 96 55
44 10
Bpu10I CC'TnA_GC
Cuts at: 0 763 1462 1586 1929
Size: 763 699 124 343
Fragments arranged by size:
763 699 343 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1862 1929
Size: 1862 67
BsaAI yAC'GTr
Cuts at: 0 129 1411 1929
Size: 129 1282 518
Fragments arranged by size:
1282 518 129
BsaBI GATnn'nnATC
Cuts at: 0 1510 1929
Size: 1510 419
BsaHI Gr'CG_yC
Cuts at: 0 1004 1929
Size: 1004 925
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 349 379 1476 1506 1929
Size: 349 30 1097 30 423
Fragments arranged by size:
1097 423 349 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 707 1558 1789 1929
Size: 370 337 851 231 140
Fragments arranged by size:
851 370 337 231 140
BseYI C'CCAG_C
Cuts at: 0 121 1371 1897 1929
Size: 121 1250 526 32
Fragments arranged by size:
1250 526 121 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 1063 1150 1189 1929
Size: 274 789 87 39 740
Fragments arranged by size:
789 740 274 87 39
BsiEI CG_ry'CG
Cuts at: 0 398 1139 1929
Size: 398 741 790
Fragments arranged by size:
790 741 398
BsiHKAI G_wGCw'C
Cuts at: 0 20 593 925 1489 1754 1929
Size: 20 573 332 564 265 175
Fragments arranged by size:
573 564 332 265 175 20
BsmBI CGTCTCn'nnnn_
Cuts at: 0 385 1929
Size: 385 1544
Bsp1286I G_dGCh'C
Cuts at: 0 20 43 446 506 533 593 616 783
Size: 20 23 403 60 27 60 23 167
Cuts at: 783 906 925 1024 1489 1754 1929
Size: 123 19 99 465 265 175
Fragments arranged by size:
465 403 265 175 167 123 99 60
60 27 23 23 20 19
BspHI T'CATG_A
Cuts at: 0 999 1563 1929
Size: 999 564 366
Fragments arranged by size:
999 564 366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1213 1376 1929
Size: 1213 163 553
Fragments arranged by size:
1213 553 163
BsrBI CCG'CTC
Cuts at: 0 150 767 1929
Size: 150 617 1162
Fragments arranged by size:
1162 617 150
BsrFI r'CCGG_y
Cuts at: 0 563 710 951 1529 1903 1929
Size: 563 147 241 578 374 26
Fragments arranged by size:
578 563 374 241 147 26
BssHII G'CGCG_C
Cuts at: 0 810 1929
Size: 810 1119
BssSI C'ACGA_G
Cuts at: 0 51 715 899 1929
Size: 51 664 184 1030
Fragments arranged by size:
1030 664 184 51
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1050 1929
Size: 1050 879
BstEII G'GTnAC_C
Cuts at: 0 1431 1929
Size: 1431 498
BstXI CCAn_nnnn'nTGG
Cuts at: 0 129 1929
Size: 129 1800
BstYI r'GATC_y
Cuts at: 0 86 359 1173 1929
Size: 86 273 814 756
Fragments arranged by size:
814 756 273 86
Bsu36I CC'TnA_GG
Cuts at: 0 1678 1929
Size: 1678 251
BtgI C'CryG_G
Cuts at: 0 10 248 327 368 533 1929
Size: 10 238 79 41 165 1396
Fragments arranged by size:
1396 238 165 79 41 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 480 531 1929
Size: 480 51 1398
Fragments arranged by size:
1398 480 51
BtsI GCAGTG_nn'
Cuts at: 0 437 668 1929
Size: 437 231 1261
Fragments arranged by size:
1261 437 231
DraIII CAC_nnn'GTG
Cuts at: 0 432 1929
Size: 432 1497
DrdI GACnn_nn'nnGTC
Cuts at: 0 649 1929
Size: 649 1280
EaeI y'GGCC_r
Cuts at: 0 92 167 395 1136 1929
Size: 92 75 228 741 793
Fragments arranged by size:
793 741 228 92 75
EagI C'GGCC_G
Cuts at: 0 395 1136 1929
Size: 395 741 793
Fragments arranged by size:
793 741 395
EarI CTCTTCn'nnn_
Cuts at: 0 937 1691 1786 1929
Size: 937 754 95 143
Fragments arranged by size:
937 754 143 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 759 1929
Size: 759 1170
EcoICRI GAG'CTC
Cuts at: 0 18 1929
Size: 18 1911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 816 871 967 1234 1651 1768 1812
Size: 695 121 55 96 267 417 117 44
Cuts at: 1812 1822 1929
Size: 10 107
Fragments arranged by size:
695 417 267 121 117 107 96 55
44 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 868 1929
Size: 868 1061
EcoO109I rG'GnC_Cy
Cuts at: 0 6 759 963 1843 1893 1929
Size: 6 753 204 880 50 36
Fragments arranged by size:
880 753 204 50 36 6
EcoRI G'AATT_C
Cuts at: 0 736 1929
Size: 736 1193
FspI TGC'GCA
Cuts at: 0 787 796 1929
Size: 787 9 1133
Fragments arranged by size:
1133 787 9
HaeII r_GCGC'y
Cuts at: 0 471 1929
Size: 471 1458
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 381 1929
Size: 349 32 1548
Fragments arranged by size:
1548 349 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1929
Size: 239 1690
MscI TGG'CCA
Cuts at: 0 94 1929
Size: 94 1835
MslI CAynn'nnrTG
Cuts at: 0 250 1327 1929
Size: 250 1077 602
Fragments arranged by size:
1077 602 250
MspA1I CmG'CkG
Cuts at: 0 329 541 1761 1929
Size: 329 212 1220 168
Fragments arranged by size:
1220 329 212 168
NaeI GCC'GGC
Cuts at: 0 1531 1929
Size: 1531 398
NcoI C'CATG_G
Cuts at: 0 368 1929
Size: 368 1561
NgoMIV G'CCGG_C
Cuts at: 0 1529 1929
Size: 1529 400
PasI CC'CwG_GG
Cuts at: 0 1855 1929
Size: 1855 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 729 1929
Size: 729 1200
PmlI CAC'GTG
Cuts at: 0 1411 1929
Size: 1411 518
PpuMI rG'GwC_Cy
Cuts at: 0 759 1893 1929
Size: 759 1134 36
Fragments arranged by size:
1134 759 36
PshAI GACnn'nnGTC
Cuts at: 0 1724 1929
Size: 1724 205
PstI C_TGCA'G
Cuts at: 0 1058 1929
Size: 1058 871
PvuII CAG'CTG
Cuts at: 0 541 1761 1929
Size: 541 1220 168
Fragments arranged by size:
1220 541 168
SacI G_AGCT'C
Cuts at: 0 20 1929
Size: 20 1909
SacII CC_GC'GG
Cuts at: 0 330 1929
Size: 330 1599
SanDI GG'GwC_CC
Cuts at: 0 1893 1929
Size: 1893 36
SfcI C'TryA_G
Cuts at: 0 1054 1929
Size: 1054 875
SmlI C'TyrA_G
Cuts at: 0 1877 1929
Size: 1877 52
StyI C'CwwG_G
Cuts at: 0 308 368 479 1037 1355 1929
Size: 308 60 111 558 318 574
Fragments arranged by size:
574 558 318 308 111 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 853 1929
Size: 595 258 1076
Fragments arranged by size:
1076 595 258
TatI w'GTAC_w
Cuts at: 0 1577 1929
Size: 1577 352
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 762 794 1400 1432 1929
Size: 762 32 606 32 497
Fragments arranged by size:
762 606 497 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1006 1929
Size: 1006 923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1626 1929
Size: 1626 303
XmnI GAAnn'nnTTC
Cuts at: 0 724 1929
Size: 724 1205
ZraI GAC'GTC
Cuts at: 0 1005 1929
Size: 1005 924
Enzymes that do cut:
AarI AatII AflIII AleI AloI AlwNI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PmlI
PpuMI PshAI PstI PvuII SacI SacII SanDI SfcI
SmlI StyI TaqII TatI TstI Tth111I XcmI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BfrBI BmrI BmtI BsaI BsaWI
BsiWI BsmI BspEI BsrDI BsrGI BstBI BstZ17I ClaI
CspCI DraI EcoRV FalI FseI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI XmaI