Restriction map for
pFN21AE4546
(Linear) (Six-base) MAPSORT of: KIEE4546.seq Check: 3242 from: 1 to: 1920
KIEE4546.orf
With 182 enzymes: SgfI *
March 22, 2012 18:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 252 1920
Size: 252 1668
AatII G_ACGT'C
Cuts at: 0 126 1920
Size: 126 1794
AccI GT'mk_AC
Cuts at: 0 970 1448 1920
Size: 970 478 472
Fragments arranged by size:
970 478 472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1754 1920
Size: 1754 166
AflIII A'CryG_T
Cuts at: 0 1790 1920
Size: 1790 130
AgeI A'CCGG_T
Cuts at: 0 1502 1920
Size: 1502 418
AhdI GACnn_n'nnGTC
Cuts at: 0 390 1920
Size: 390 1530
AlwNI CAG_nnn'CTG
Cuts at: 0 90 1920
Size: 90 1830
ApoI r'AATT_y
Cuts at: 0 292 417 821 1751 1920
Size: 292 125 404 930 169
Fragments arranged by size:
930 404 292 169 125
AseI AT'TA_AT
Cuts at: 0 1684 1920
Size: 1684 236
BamHI G'GATC_C
Cuts at: 0 347 1656 1920
Size: 347 1309 264
Fragments arranged by size:
1309 347 264
BanI G'GyrC_C
Cuts at: 0 204 1920
Size: 204 1716
BbsI GAAGACnn'nnnn_
Cuts at: 0 1301 1308 1920
Size: 1301 7 612
Fragments arranged by size:
1301 612 7
BclI T'GATC_A
Cuts at: 0 687 1343 1920
Size: 687 656 577
Fragments arranged by size:
687 656 577
BglI GCCn_nnn'nGGC
Cuts at: 0 202 1920
Size: 202 1718
BlpI GC'TnA_GC
Cuts at: 0 91 179 1920
Size: 91 88 1741
Fragments arranged by size:
1741 91 88
Bme1580I G_kGCm'C
Cuts at: 0 43 893 1920
Size: 43 850 1027
Fragments arranged by size:
1027 850 43
BmgBI CAC'GTC
Cuts at: 0 44 1920
Size: 44 1876
BmrI ACTGGGnnnn_n'
Cuts at: 0 1454 1557 1819 1920
Size: 1454 103 262 101
Fragments arranged by size:
1454 262 103 101
BmtI G_CTAG'C
Cuts at: 0 463 1920
Size: 463 1457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 169 1920
Size: 169 1751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1109 1610 1920
Size: 139 970 501 310
Fragments arranged by size:
970 501 310 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1920
Size: 679 1241
BsaI GGTCTCn'nnnn_
Cuts at: 0 140 1920
Size: 140 1780
BsaAI yAC'GTr
Cuts at: 0 1096 1920
Size: 1096 824
BsaBI GATnn'nnATC
Cuts at: 0 1040 1348 1920
Size: 1040 308 572
Fragments arranged by size:
1040 572 308
BsaHI Gr'CG_yC
Cuts at: 0 123 1920
Size: 123 1797
BsaWI w'CCGG_w
Cuts at: 0 1502 1920
Size: 1502 418
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1687 1920
Size: 1687 233
BsiHKAI G_wGCw'C
Cuts at: 0 1867 1920
Size: 1867 53
Bsp1286I G_dGCh'C
Cuts at: 0 43 893 1867 1920
Size: 43 850 974 53
Fragments arranged by size:
974 850 53 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 252 1920
Size: 252 1668
BsrFI r'CCGG_y
Cuts at: 0 149 1502 1920
Size: 149 1353 418
Fragments arranged by size:
1353 418 149
BstAPI GCAn_nnn'nTGC
Cuts at: 0 24 1920
Size: 24 1896
BstEII G'GTnAC_C
Cuts at: 0 478 1498 1920
Size: 478 1020 422
Fragments arranged by size:
1020 478 422
BstXI CCAn_nnnn'nTGG
Cuts at: 0 900 1920
Size: 900 1020
BstYI r'GATC_y
Cuts at: 0 347 855 1656 1920
Size: 347 508 801 264
Fragments arranged by size:
801 508 347 264
BstZ17I GTA'TAC
Cuts at: 0 971 1920
Size: 971 949
DraI TTT'AAA
Cuts at: 0 1800 1920
Size: 1800 120
DrdI GACnn_nn'nnGTC
Cuts at: 0 721 1444 1920
Size: 721 723 476
Fragments arranged by size:
723 721 476
EaeI y'GGCC_r
Cuts at: 0 194 222 1920
Size: 194 28 1698
Fragments arranged by size:
1698 194 28
EarI CTCTTCn'nnn_
Cuts at: 0 662 1920
Size: 662 1258
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 158 1920
Size: 158 1762
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1109 1610 1754 1920
Size: 139 970 501 144 166
Fragments arranged by size:
970 501 166 144 139
EcoO109I rG'GnC_Cy
Cuts at: 0 723 1920
Size: 723 1197
FspI TGC'GCA
Cuts at: 0 263 1920
Size: 263 1657
HaeII r_GCGC'y
Cuts at: 0 1621 1920
Size: 1621 299
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 1851 1883 1920
Size: 376 32 1443 32 37
Fragments arranged by size:
1443 376 37 32 32
HincII GTy'rAC
Cuts at: 0 329 552 1920
Size: 329 223 1368
Fragments arranged by size:
1368 329 223
HindIII A'AGCT_T
Cuts at: 0 1766 1920
Size: 1766 154
MfeI C'AATT_G
Cuts at: 0 113 1920
Size: 113 1807
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 757 941 1920
Size: 151 606 184 979
Fragments arranged by size:
979 606 184 151
MscI TGG'CCA
Cuts at: 0 196 224 1920
Size: 196 28 1696
Fragments arranged by size:
1696 196 28
MspA1I CmG'CkG
Cuts at: 0 168 456 1920
Size: 168 288 1464
Fragments arranged by size:
1464 288 168
NheI G'CTAG_C
Cuts at: 0 459 1920
Size: 459 1461
NspI r_CATG'y
Cuts at: 0 268 393 765 1794 1920
Size: 268 125 372 1029 126
Fragments arranged by size:
1029 372 268 126 125
PciI A'CATG_T
Cuts at: 0 1790 1920
Size: 1790 130
PflMI CCAn_nnn'nTGG
Cuts at: 0 133 1920
Size: 133 1787
PpuMI rG'GwC_Cy
Cuts at: 0 723 1920
Size: 723 1197
PsiI TTA'TAA
Cuts at: 0 1151 1920
Size: 1151 769
PvuII CAG'CTG
Cuts at: 0 168 1920
Size: 168 1752
SfcI C'TryA_G
Cuts at: 0 96 1430 1920
Size: 96 1334 490
Fragments arranged by size:
1334 490 96
SmlI C'TyrA_G
Cuts at: 0 694 1920
Size: 694 1226
SpeI A'CTAG_T
Cuts at: 0 1164 1920
Size: 1164 756
StyI C'CwwG_G
Cuts at: 0 588 1011 1398 1838 1920
Size: 588 423 387 440 82
Fragments arranged by size:
588 440 423 387 82
TatI w'GTAC_w
Cuts at: 0 1272 1920
Size: 1272 648
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 992 1024 1920
Size: 992 32 896
Fragments arranged by size:
992 896 32
XbaI T'CTAG_A
Cuts at: 0 375 1920
Size: 375 1545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 900 1920
Size: 900 1020
XmnI GAAnn'nnTTC
Cuts at: 0 317 1920
Size: 317 1603
ZraI GAC'GTC
Cuts at: 0 124 1920
Size: 124 1796
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AhdI AlwNI
ApoI AseI BamHI BanI BbsI BclI BglI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsgI BsiHKAI Bsp1286I BspMI
BsrFI BstAPI BstEII BstXI BstYI BstZ17I DraI DrdI
EaeI EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I
HincII HindIII MfeI MmeI MscI MspA1I NheI NspI
PciI PflMI PpuMI PsiI PvuII SfcI SmlI SpeI
StyI TatI TstI XbaI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AleI AlfI AloI ApaI
ApaLI AscI AsiSI AvaI AvrII BaeI BanII BbeI
BbvCI BcgI BciVI BfrBI BglII Bpu10I BsaXI BseRI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstBI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspAI HpaI KasI KpnI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI
SwaI TaqII Tth111I XhoI XmaI